支原体DNA构象转变的热力学参数。

Mikrobiologicheskii zhurnal Pub Date : 1991-03-01
L P Panchenko, V A Turchin, I G Skripal'
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引用次数: 0

摘要

从10株植物致病性支原体中分离得到了DNA制剂,并收集培养了Achole plasma lailawii PG-8。已经确定了这些支原体DNA二级结构(转化构象)变性变化的热力学参数。结果表明,变性温度为82.3 ~ 83.1℃,每g干物质计算的构象DNA转变焓为56.2 ~ 61.9 J/g。每mol核苷酸对的焓(H)和熵(S)的变化范围在35.6 ~ 39.0 J/m.p之间。和995 ~ 109.6 J°m.p.。研究结果显示,支原体的热吸附与DNA变性温度之间没有线性关系,这可能与支原体DNA的结构特性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The thermodynamic parameters of the conformational transitions in Mycoplasma DNA].

DNA preparations have been isolated from 10 strains of phytopathogenic mycoplasms and collection culture Achole plasma laidlawii PG-8. Thermodynamic parameters of denaturation changes in the secondary structure (transconformation) of DNA of these mycoplasms have been determined. It is shown that denaturation temperature is 82.3-83.1 degree C and enthalpy of conformational DNA transitions calculated per 1 g of dry substance is 56.2-61.9 J/g. Changes in the enthalpy (delta H) and entropy (delta S) calculated per 1 mol of nucleotide pairs varied in the range of 35.6-39.0 J/m.p. and 995-109.6 J degree m.p. respectively. No linear dependence of transconformational thermal adsorption on the temperature of DNA denaturation of the studied strains of mycoplasms are revealed, which is probably connected with structural peculiarities of DNA of these microorganisms.

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