[The effect of physicochemical factors on the growth of Pseudomonas putida BS-2 on a medium with diethylene glycol].

Mikrobiologicheskii zhurnal Pub Date : 1992-07-01
S A Sedina
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Abstract

The physicochemical factors of medium have been studied for their effect on the physiological indices of growth of Pseudomonas putida BS-2 culture utilizing diethylenglycol as the only source of carbon. Action of the supraoptimal temperature on the growth process of P. putida BS-2 is accompanied by a decrease (more than twice) in economic coefficient of substrate and specific growth rate as compared with their maximal values. Dependences of specific growth rate of P. putida BS-2 in the medium with diethylenglycol on the presence of NaCl in it within the range of its concentrations from 0 to 4% and methanol in the concentration range of 0-20 g/l follow the noncompetitive inhibition equation. When NaCl concentration in the medium is more than 4%, complete separation of constructive and energy metabolism processes is observed.

[物化因素对恶臭假单胞菌BS-2在二甘醇培养基上生长的影响]。
以二乙二醇为唯一碳源,研究了培养基理化因素对恶臭假单胞菌BS-2生长生理指标的影响。超优温度对恶臭p.p . putida BS-2生长过程的影响伴随着基质经济系数和比生长率较其最大值降低2倍以上。P. putida BS-2在含二乙基乙二醇的培养基中生长速率取决于NaCl在0 ~ 4%的浓度范围内和甲醇在0 ~ 20 g/l的浓度范围内的存在,符合非竞争性抑制方程。当培养基中NaCl浓度大于4%时,构建过程和能量代谢过程完全分离。
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