Experimentelle Pathologie最新文献

{"title":"The effect of exercise on myocardial interstitium. An ultrastructural morphometric study","authors":"H. Guski","doi":"10.1016/S0014-4908(80)80013-5","DOIUrl":"10.1016/S0014-4908(80)80013-5","url":null,"abstract":"<div><p>Ultrastructural morphometric examinations show that the normal rat myocardium of the left ventricle contains about 15% interstitial connective tissue, half of which (53%) is composed of extracellular space, ground-substance and collagen, 41% of blood capillaries and 6% of interstitial cells. These percentages vary in the course of swimming training for periods of 45, 180 and 360 hours in dependence on the duration of training and load. The volume ratio of the interstitium increases after 45 hours of exercise from 15 to 17 %, after 180 hours of training to 19% and after 360 hours of training to 22%. Relative changes in morphometric parameters (volume and surface density, number per unit area) of the interstitial components are observed in relation to untrained animals. Owing to a shift in the volume ratio of interstitium to muscle cells in favour of the interstitium, the real volume ratio changes only in the case of extracellular space and capillaries.</p><p>After 45 hours of training the volume ratio of extracellular space to interstitium decreases to 50%, that of the capillaries increases to 44%. After 180 hours of training the volume ratio of blood capillaries diminishes to 32%, while that of extracellular space increases (62.5%). This ratio remains constant in the course of further training even if the load is increased. Exercise has virtually no bearing on the volume ratio of connective tissue cells.</p><p>There is an increase in the capillary density of the myocardium and a reduction in the extracellular space after 45 hours of training. After 180 hours of maintenance training the ratio is inversed again and after that is no longer influenced by exercise.</p><p>Unlike the number per unit area, the surface density of the capillaries increases with increasing intensity of the swimming load throughout the training period. The volume ratio of capillary to muscle cells changes in favour of the capillaries in the course of training. Specific surface density of the capillaries increases continuously up to a training period of 180 hours, and there is no further increase even if training is continued after that. The morphometrical changes in the capillaries suggest that a short, but intensive period of training brings about an increase in the capillary surface, which can be maintained but not increased through further training.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 3","pages":"Pages 141-150"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80013-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17505949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of antidiuretic hormone to the connective tissues of rat renal papilla","authors":"C.N. Sun","doi":"10.1016/S0014-4908(80)80001-9","DOIUrl":"10.1016/S0014-4908(80)80001-9","url":null,"abstract":"<div><p>We studied a strain of rats with congenital diabetes insipidus homozygote (DI). The collecting tubules could not concentrate urine. Electron microscopy shows cytoplasmic lesions, vacuoles, swelling and degenerating mitochondria in the collecting tubule cells. Heterozygous animals are similar to the normal animals and rarely show the above described lesions. The connective tissue mucopolysaccharide (MPS) in the renal papilla of normal rats stains more intensely with Alcian Blue-PAS than comparable tissue from DI rats. After vasopressin was given daily to the DI rats for a period of more than three weeks, the animals present a normal water intake, an increase in urine osmolality and an increase in MPS in the connective tissue. The cytoplasmic lesions in the collecting tubule cells also disappear. Thus, it seems reasonable to relate the morphologic changes of DI rats to a lack of appropriate ADH activity. Apparently, ADH causes alteration in the polymeric state of the MPS in connective tissue of the papilla and thereby presumably induces changes in permeability.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 9","pages":"Pages 469-473"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80001-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"55384100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carcinogenic effect of N-bis-(2-hydroxypropyl)nitrosamine in newborn rats","authors":"Y. Konishi,&nbsp;T. Ikeda,&nbsp;A. Kawabata,&nbsp;H. Katada,&nbsp;K. Higashiguchi,&nbsp;H. Maruyama,&nbsp;H. Yoshimura","doi":"10.1016/S0014-4908(80)80063-9","DOIUrl":"10.1016/S0014-4908(80)80063-9","url":null,"abstract":"<div><p>The carcinogenic effect of N-bis(2-hydroxypropyl)nitrosamine (BHP) was studied in newborn Wistar rats. Tumors were induced in the lung (adenoma), pancreas (adenoma), kidney (nephroblastoma), and ovary (benign granulosa cell tumor) at 24 weeks after the treatment and in the lung (adenoma and adenocarcinoma), liver (hemangioma, hemangioendothelioma, and hepatocellular carcinoma), pancreas (adenoma), kidney (renal cell carcinoma and nephroblastoma), thyroid (adenocarcinoma) and ovary (benign and malignant granulosa cell tumors) at 52 weeks. The highest incidence of tumor was seen in the liver of rats applied BHP within 24 hours after birth.</p><p>These results indicate that newborn Wistar rats are more susceptible to BHP than are adult rats.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 2","pages":"Pages 136-140"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80063-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18392322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diminished prostacyclin formation in forearm venous tissue of patients with peripheral occlusive arteriopathy","authors":"H. Sinzinger,&nbsp;J. Kaliman,&nbsp;K. Silberbauer,&nbsp;R. Oppolzer","doi":"10.1016/S0014-4908(80)80056-1","DOIUrl":"10.1016/S0014-4908(80)80056-1","url":null,"abstract":"","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 4","pages":"Pages 254-255"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80056-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18042520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of adrenaline-tablets on rat heart cells in tissue culture at normal and raised temperature","authors":"K. Pfragner ,&nbsp;S. Porta ,&nbsp;V. Walser ,&nbsp;W. Korsatko","doi":"10.1016/S0014-4908(80)80024-X","DOIUrl":"10.1016/S0014-4908(80)80024-X","url":null,"abstract":"<div><p>Primary cultures of rat heart cells were incubated at 37 °C or 39.5 °C. For permanent treatment with adrenaline recently developed tablets were added to the cultures. After 10 hours the cell counts, glucose, lactate, LDH, α-HBDH and GOT were determined. Permanent adrenaline-application led to a decrease of cell counts, an increase of lactate, LDH, α-HBDH and GOT. The results indicate an injured membrane function of rat heart cells. Raised temperature sensitized the cells for adrenaline-treatment.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 6","pages":"Pages 341-345"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80024-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18428334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-microscopic and fluorescent histochemical investigations on the salivary glands of rats after isoproterenol treatment","authors":"K. Tuch,&nbsp;Th. Matthiesen","doi":"10.1016/S0014-4908(80)80014-7","DOIUrl":"10.1016/S0014-4908(80)80014-7","url":null,"abstract":"<div><p>The effect of isoproterenol (IPR) on the salivary glands of the rat were investigated after single and repeated administration of 80 mg/kg body weight subcutaneously. Adrenergic nerve fibres were demonstrated with the aid of formalin-induced fluorescence, and cholinergic fibres by means of the acetylcholinesterase reaction.</p><p>After a single dose of IPR the acini of the submandibular and parotid glands were depleted and then hypertrophic and hyperplastic. Large scattered cells with retained secretory granules transiently occurred after three days' treatment. They were identified as cells which degenerated mainly during mitosis. The distribution pattern of the autonomic nerve fibres in both glands adjusted to the changes preformed by the acini in the course of treatment.</p><p>After long-term treatment there was a distinct loss of fibre structures and intensity of fluorescence. Thus it can be concluded that the autonomic fibres degenerate. The effect of IPR on the proliferation kinetics of the salivary glands is discussed. The fluorescent histochemical results are compared with the electron-microscopic findings in autonomic nerves reported in the literature.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 3","pages":"Pages 151-162"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80014-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18399345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of lymphotropic baboon herpesvirus (HVP) from oral swabs of hamadryas baboons of the Sukhumi monkey colony","authors":"V.Z. Agrba,&nbsp;B.A. Lapin,&nbsp;V.V. Timanovskaya,&nbsp;M.Ch. Dzhachvliany,&nbsp;L.V. Kokosha,&nbsp;G.N. Chuvirov,&nbsp;A.G. Djatchenko","doi":"10.1016/S0014-4908(80)80031-7","DOIUrl":"10.1016/S0014-4908(80)80031-7","url":null,"abstract":"<div><p>Ways of lymphotropic baboon herpesvirus (HVP) secretion and its excretion into the environment were investigated. Oral swabs and feces from the Sukhumi main stock hamadryas baboons characterized by a high risk for malignant lymphoma and the baboon stock living in isolation in the forest were used as materials for the investigations. Macaque groups of the Sukhumi stock were used as controls. It could be shown that the HVP was resistent in the oral cavity of the main stock baboons and was isolated from oral swabs of these animals both from those with malignant lymphoma and clinically healthy individuals. No virus was isolated from feces of these animals. The virus could not be isolated from oral swabs of the isolated baboon stock and macaques.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 5","pages":"Pages 269-274"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80031-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18419726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural and biochemical studies of the brain and other organs in rats after chronic ethanol administration","authors":"M. Kujawa ,&nbsp;H. Karwacka","doi":"10.1016/S0014-4908(80)80022-6","DOIUrl":"10.1016/S0014-4908(80)80022-6","url":null,"abstract":"<div><p>Ultrastructural and stereological analysis of mitochondria of liver cells from rats was performed after intoxication with various doses of ethanol administered for various time periods in increasing concentrations.</p><p>The following changes were observed in the cells of the experimental animals as compared with the control ones:</p><ul><li><span>-</span><span><p>a decrease of the surface area of mitochondria per unit volume of the hepatocyte,</p></span></li><li><span>-</span><span><p>a decrease of the mean number of mitochondrial profiles per 100 μm² surface area of hepatocyte cross section,</p></span></li><li><span>-</span><span><p>a decrease in the value of the axial ratio of mitochondrial profiles</p></span></li></ul><p>A diminished volume fraction of the mitochondria was not observed.</p><p>The above presented results show that, after ethanol poisoning, the mitochondria in hepatic cells become enlarged and rounded and their number seems to diminish.</p><p>Qualitative studies demonstrated that the structure of mitochondria is frequently damaged, this consisting in injury to their membranes, formation of lamellar structures, and destruction of the system of cristae and matrix. This leads to the presence of degenerated, sometimes monstrously large mitochondria in the cells.</p><p>The above described changes occurred as early as after 4 weeks of ethanol administration in increasing doses from 2.5 to 10 per cent.</p><p>An increase of ethanol concentration to 42 per cent and prolongation of the period of treatment with ethanol to 12 weeks did not markedly enhance the changes.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 6","pages":"Pages 329-335"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80022-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18205770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phagocytosis of inert particles in the reticuloendothelial system in rats with experimental insulinopenic diabetes","authors":"E. Schneider,&nbsp;U. Fuchs,&nbsp;G. Schneider,&nbsp;W. Helbig","doi":"10.1016/S0014-4908(80)80073-1","DOIUrl":"10.1016/S0014-4908(80)80073-1","url":null,"abstract":"<div><p>Phagocytosis of inert, radioactive gold particles in the reticuloendothelial system of rats in the case of experimental insulinopenic diabetes was studied. In the spleen, phagocytosis was seen to have fallen to a relatively low level after four weeks from the induction of diabetes. In the liver, there was noted a reduction in phagocytosis per gram of organ, though it remained the same for the total organ which in the case of diabetes mellitus increases in weight relative to body weight. This difference in reaction of the reticuloendothelial system in the liver and spleen has also been observed in mice with tumors. The importance of the findings of the present study for increased sensitivity to infections of poorly controlled diabetic patients is discussed in greater detail.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 1","pages":"Pages 75-79"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80073-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17310052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light and electron microscopic evaluation of thymuses from feline leukemia virus-infected kittens","authors":"F.D. Pack,&nbsp;W.L. Chapman","doi":"10.1016/S0014-4908(80)80058-5","DOIUrl":"10.1016/S0014-4908(80)80058-5","url":null,"abstract":"<div><p>Light microscopic and electron microscopic findings in thymuses from 4-week old feline leukemia virus-infected and 4- and 9-week old noninfected kittens were evaluated and found to be morphologically similar to each other. Thymuses from 9-week old feline leukemia virusinfected kittens were markedly atrophied and individual lobules within each thymus varied in the severity of atrophy. Lobules having the least severe atrophy had a moderate thinning of the cortex and a heterogeneous thymocyte population arranged in disorganized cords. Additional morphologic changes in these thymuses included intense eosinopoiesis at the corticomedullary junction, increased prominence of vasculature, and enlarged Hassal's corpuscles. In addition to these changes lobules of thymus having the most severe atrophy had a marked cortical thymocyte depletion, lobule collapse, and increased numbers of mast cells. Degeneration of epithelial cells in most lobules was indicated by electronlucency of the cytoplasmic matrix and often greatly dilated rough endoplasmic reticulum.</p></div>","PeriodicalId":75841,"journal":{"name":"Experimentelle Pathologie","volume":"18 2","pages":"Pages 96-110"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0014-4908(80)80058-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17310053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}