Effects of adrenaline-tablets on rat heart cells in tissue culture at normal and raised temperature

Abstract

Primary cultures of rat heart cells were incubated at 37 °C or 39.5 °C. For permanent treatment with adrenaline recently developed tablets were added to the cultures. After 10 hours the cell counts, glucose, lactate, LDH, α-HBDH and GOT were determined. Permanent adrenaline-application led to a decrease of cell counts, an increase of lactate, LDH, α-HBDH and GOT. The results indicate an injured membrane function of rat heart cells. Raised temperature sensitized the cells for adrenaline-treatment.