Endocrine research communications最新文献

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Effects of (-)-trans- delta 9-tetrahydrocannabinol on serum prolactin in the pseudopregnant rat. (-)-反式- δ 9-四氢大麻酚对假妊娠大鼠血清催乳素的影响。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045750
C L Hughes, L Tyrey
{"title":"Effects of (-)-trans- delta 9-tetrahydrocannabinol on serum prolactin in the pseudopregnant rat.","authors":"C L Hughes,&nbsp;L Tyrey","doi":"10.1080/07435808209045750","DOIUrl":"https://doi.org/10.1080/07435808209045750","url":null,"abstract":"<p><p>Groups of pseudopregnant rats were injected intravenously with (-)-trans- delta 9-tetrahydrocannabinol (THC) to determine its effects on serum prolactin (PRL) and the maintenance of pseudopregnancy. A single injection of 4 mg THC/kg BW at 2400 h on the first day of leukocytic vaginal smears of pseudopregnancy (D-1) delayed the ensuing nocturnal PRL surge for approximately one hour. When THC (1.0 mg/kg BW) was administered hourly from 2400 h on D-1 through 1700 h on D-2, the nocturnal surge was blocked and serum PRL levels were suppressed until 0600 h on D-2, but not thereafter. Neither treatment altered the duration of pseudopregnancy. These results indicate that the nocturnal surge secretion of PRL during early pseudopregnancy in the rat is sensitive to THC suppression, but that this suppression is not adequate to influence the duration of pseudopregnancy. The mechanism through which THC exerts this action remains unknown.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 1","pages":"25-36"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045750","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17346488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Genetic variation in testicular LH receptors in the mouse. 小鼠睾丸LH受体的遗传变异。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045755
A Amador, A Bartke, W Beamer
{"title":"Genetic variation in testicular LH receptors in the mouse.","authors":"A Amador,&nbsp;A Bartke,&nbsp;W Beamer","doi":"10.1080/07435808209045755","DOIUrl":"https://doi.org/10.1080/07435808209045755","url":null,"abstract":"<p><p>The concentration of testicular hCG (LH) binding sites was measured in five mutants with defective endocrine and/or reproductive functions, in a hypoandrogenic inbred strain and in six normal strains. A decrease in hCG binding was found in dw/dw mice, and an increase in Tfm/y and C57Bl10/J mice. Analysis of the results indicates the existence of a genetic factor(s) involved in the regulation of hCG (LH) binding.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 2","pages":"79-88"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045755","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17359794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Effect of neurotensin on specific activity of plasma cholesterol following intravenous administration of cholesterol-4-14C. 静脉注射胆固醇-4- 14c后神经紧张素对血浆胆固醇比活性的影响。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.3109/07435808209045763
L Peric-Golia, C F Gardner, M Peric-Golia
{"title":"Effect of neurotensin on specific activity of plasma cholesterol following intravenous administration of cholesterol-4-14C.","authors":"L Peric-Golia,&nbsp;C F Gardner,&nbsp;M Peric-Golia","doi":"10.3109/07435808209045763","DOIUrl":"https://doi.org/10.3109/07435808209045763","url":null,"abstract":"<p><p>In order to investigate the source of the additional circulating cholesterol in hypercholesterolemia induced by neurotensin (NT), 12.5 pmol/100 g body weight NT was injected into the tail veins of rats which received by the same route 1 X 10(6) dpm/100 g body weight cholesterol-4-14C 24 hours earlier. Fifteen minutes later the concentration of the labeled plasma cholesterol was the same in the treated animals and in the controls. However, specific activity of plasma cholesterol significantly decreased in rats that received NT, while it remained unchanged in the controls. These data suggest that the additional circulating cholesterol after the intravenous administration of NT was either newly formed or originated in some tissue where equilibrium between endogenous and exogenous cholesterol has not yet been established.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 3-4","pages":"179-84"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808209045763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18203769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A sensitive method for detection of circulating forms of human growth hormone by immunoautoradiography. 免疫放射自显影法检测人生长激素循环形式的灵敏方法。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.3109/07435808209045767
D A Cohen, K O'Connell, S L Blethen, W H Daughaday
{"title":"A sensitive method for detection of circulating forms of human growth hormone by immunoautoradiography.","authors":"D A Cohen,&nbsp;K O'Connell,&nbsp;S L Blethen,&nbsp;W H Daughaday","doi":"10.3109/07435808209045767","DOIUrl":"https://doi.org/10.3109/07435808209045767","url":null,"abstract":"<p><p>Several different forms of hGH have been isolated from the pituitary, but little is known about modifications of synthesized forms that may exist in the circulation. We used a combination of serum immunoextraction with gel electrophoresis and electrophoretic blotting to detect circulating hGH isohormones. Three different components were identified, with the predominant form being 22,000 MW isomer. The method is sensitive to nanogram quantities of hGH and is applicable to the screening of human serum for the presence of variants of hGH.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 3-4","pages":"233-47"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808209045767","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17817081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential effect of althesin versus chloralose-urethane anesthesia on in vivo LHRH and LH release in the proestrous rat. 醛糖素与氯醛脲麻醉对发情大鼠体内LHRH和LH释放的差异影响。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045751
M Ching
{"title":"Differential effect of althesin versus chloralose-urethane anesthesia on in vivo LHRH and LH release in the proestrous rat.","authors":"M Ching","doi":"10.1080/07435808209045751","DOIUrl":"https://doi.org/10.1080/07435808209045751","url":null,"abstract":"<p><p>Anesthetic doses of Althesin and chloralose-urethane were compared as to their effects on neuroendocrine reproductive processes in diestrous and proestrous rats. Proestrous rats treated with Althesin experienced a 5-fold increase in immuno-reactive luteinizing hormone releasing hormone (LHRH) concentration in methanolic extracts of pituitary stalk plasma. This LHRH increase was 67% greater than that of proestrous rats administered chloralose-urethane. Moreover, Althesin given before the onset of the critical period did not suppress the proestrous surge of LHRH in stalk plasma. The Duncan's multiple range test indicated stalk plasma LHRH activity was significantly elevated in proestrous rats anesthetized with Althesin (p less than 0.05) but not with chloralose-urethane. However, the luteinizing hormone (LH) concentration in peripheral plasma increased 14 to 18-fold in all proestrous rats, including those anesthetized with chloralose-urethane. Evidently, in chloralose-urethane-treated rats, the smaller LHRH surge during proestrus is sufficient to elicit maximum release of LH from the anterior pituitary gland. This suggest that under normal conditions the amount of LHRH secreted into portal blood during proestrus is approximately 2-fold greater than that necessary to elicit maximum secretion of LH. The results also show that picogram concentrations of LH in the stalk plasma will stimulate the release of nanogram quantities of LH into the peripheral plasma of the same rats. Moreover, the sensitivity of the pituitary gland to LHRH stimulation via the portal circulation increases nearly 3-fold between diestrus and proestrus.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 1","pages":"37-46"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045751","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18093797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Kinetics of the double-antibody, liquid-phase radioimmunoassay for rat gonadotropins. 大鼠促性腺激素双抗体液相放射免疫测定动力学。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045756
W E Findley, A Steinberger
{"title":"Kinetics of the double-antibody, liquid-phase radioimmunoassay for rat gonadotropins.","authors":"W E Findley,&nbsp;A Steinberger","doi":"10.1080/07435808209045756","DOIUrl":"https://doi.org/10.1080/07435808209045756","url":null,"abstract":"<p><p>The liquid phase, double antibody radioimmunoassays for rat LH and FSH were studied with respect to the minimum duration required for their completion using NIAMDD (National Institute of Arthritis, Metabolism and Digestive Diseases) reagents at their recommended dilutions. The minimum assay duration, which still allows maximum sensitivity, at temperatures not exceeding room temperature is 3 days for the LH assay and 4 days for the FSH assay. The extra day for the FSH assay is due to apparently nonreversible binding of rat FSH to anti-rat FSH. Such nonequilibrium binding was also observed under certain conditions with the LH assay. The greatest tracer binding was observed at a I-Ab (rabbit anti-LH or anti-FSH) incubation of 1 day at 27 degrees C plus 1 day at 4 degrees C followed by 1 additional day at 4 degrees C with the II-Ab (antirabbit gamma-globulin).</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 2","pages":"89-105"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045756","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17871029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Effects of liposome-adipocyte interaction on calcium binding and insulin action. 脂质体-脂肪细胞相互作用对钙结合和胰岛素作用的影响。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045757
A Sandra, D J Fyler
{"title":"Effects of liposome-adipocyte interaction on calcium binding and insulin action.","authors":"A Sandra,&nbsp;D J Fyler","doi":"10.1080/07435808209045757","DOIUrl":"https://doi.org/10.1080/07435808209045757","url":null,"abstract":"<p><p>The interaction of phosphatidylserine (PS)-containing liposomes with isolated rat adipocytes under conditions of liposome-cell fusion or endocytosis was accompanied by an increase in adipocyte membrane calcium binding in both the basal and insulin-stimulated state. In vesicle-treated adipocytes, insulin-stimulated 2-deoxyglucose uptake was inhibited and not as dependent on external calcium concentration as in control cells. These data suggest that the inhibition of insulin-stimulated 2-deoxyglucose uptake in vesicle treated adipocytes may result from the binding of calcium to the exogenously introduced phospholipid at the expense of membrane constituents normally involved in insulin-stimulated calcium binding. This is supported by the observations that 1) the calcium ionophore A23187 increased intracellular calcium levels, yet had no effect on insulin-stimulated hexose uptake; and 2) treatment of intact adipocytes with phospholipase A2 after the cells had been incubated with vesicles resulted in a decrease in membrane associated Ca++ and exogenous phosphatidylserine, which is consistent with a direct role of membrane phosphatidylserine in calcium binding.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 2","pages":"107-20"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045757","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17871028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
cAMP-dependent protein kinase II interactions with nuclei derived from rat mammary tumor. camp依赖性蛋白激酶II与大鼠乳腺肿瘤细胞核的相互作用。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.3109/07435808209045761
F Y Tang, L S Catapano
{"title":"cAMP-dependent protein kinase II interactions with nuclei derived from rat mammary tumor.","authors":"F Y Tang,&nbsp;L S Catapano","doi":"10.3109/07435808209045761","DOIUrl":"https://doi.org/10.3109/07435808209045761","url":null,"abstract":"<p><p>cAMP-dependent protein kinase from bovine heart exhibited significant interactions with nuclei from rat mammary tumors. These enzyme-nuclear interactions occurred when the intact holoenzyme was preincubated with 10 nM cAMP, 1 mM MgATP at 24 degrees C to produce high-affinity monophasic cAMP-dissociation kinetics. The enzyme-nuclear interactions are correlated with the loss of cAMP and PO4 from the purified enzyme. The data indicate that the high affinity cAMP-dependent protein kinase II exhibits significant nuclear interaction which may be related to cAMP function in rat mammary tumors.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 3-4","pages":"155-68"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808209045761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18032155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroendocrine control of ovarian autografts. 卵巢自体移植物的神经内分泌控制。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.3109/07435808209045764
D M Nance, W H Moger, M Wilkinson
{"title":"Neuroendocrine control of ovarian autografts.","authors":"D M Nance,&nbsp;W H Moger,&nbsp;M Wilkinson","doi":"10.3109/07435808209045764","DOIUrl":"https://doi.org/10.3109/07435808209045764","url":null,"abstract":"<p><p>At 24 days of age, either one or both ovaries of female rats were removed and transplanted subcutaneously on their respective side. Relative to sham operated rats, endocrine effects of the ovarian grafts were as follows: 1) Animals with ovarian grafts showed an advancement of puberty, 2) rats with bilateral grafts showed more 5 day estrous cycles and higher FSH levels, 3) hemi-ovariectomy of animals with bilateral grafts produced a greater increase in both LH and FSH 6-8 hr post-surgery in contrast to controls which only showed an increase in serum FSH levels, 4) weights of the bilateral grafts were comparable to intact ovaries, but the unilateral grafts were small and comparable to their original size at the time of transplantation (the intact ovary showing compensatory hypertrophy), 5) both control and bilateral ovarian graft groups showed ovarian compensatory hypertrophy, 6) removal of the small unilateral ovarian graft had no detectable endocrine effect on the remaining intact ovary which had already undergone compensatory hypertrophy, 7) removal of the intact ovary of rats with a unilateral graft produced a remarkable increase in the weight of the remaining small autograft such that these animals had the largest remaining gonad as well as higher uterine weights than controls. Thus, although ovarian autografts appear to function similar to in situ glands, the alterations in puberty, estrous cycles, FSH and LH levels and uterine weights suggest that endocrine control mechanisms are altered in rats with ovarian grafts.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 3-4","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808209045764","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17871814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Uterine hydrogen peroxide concentrations and peroxidase activities in the estrous cycle and perimaturation period of mice. 小鼠发情周期和催熟期子宫过氧化氢浓度和过氧化物酶活性。
Endocrine research communications Pub Date : 1982-01-01 DOI: 10.1080/07435808209045752
J Katz, S Kadner, M Levitz
{"title":"Uterine hydrogen peroxide concentrations and peroxidase activities in the estrous cycle and perimaturation period of mice.","authors":"J Katz,&nbsp;S Kadner,&nbsp;M Levitz","doi":"10.1080/07435808209045752","DOIUrl":"https://doi.org/10.1080/07435808209045752","url":null,"abstract":"<p><p>The concentrations of hydrogen peroxide (H2O2) and peroxidase activity (PA) were measured in mouse uterus during the perimaturation period (4-8 weeks of age) and the estrous cycle. The onset of maturation was accompanied by a spurt in PA but not in H2O2. Neither H2O2 nor PA varied significantly during the estrous cycle. These results differ from those reported in the rat where positive correlations for both with the estrogen state of the uterus were observed.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 1","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435808209045752","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18216735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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