{"title":"camp依赖性蛋白激酶II与大鼠乳腺肿瘤细胞核的相互作用。","authors":"F Y Tang, L S Catapano","doi":"10.3109/07435808209045761","DOIUrl":null,"url":null,"abstract":"<p><p>cAMP-dependent protein kinase from bovine heart exhibited significant interactions with nuclei from rat mammary tumors. These enzyme-nuclear interactions occurred when the intact holoenzyme was preincubated with 10 nM cAMP, 1 mM MgATP at 24 degrees C to produce high-affinity monophasic cAMP-dissociation kinetics. The enzyme-nuclear interactions are correlated with the loss of cAMP and PO4 from the purified enzyme. The data indicate that the high affinity cAMP-dependent protein kinase II exhibits significant nuclear interaction which may be related to cAMP function in rat mammary tumors.</p>","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"9 3-4","pages":"155-68"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435808209045761","citationCount":"0","resultStr":"{\"title\":\"cAMP-dependent protein kinase II interactions with nuclei derived from rat mammary tumor.\",\"authors\":\"F Y Tang, L S Catapano\",\"doi\":\"10.3109/07435808209045761\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>cAMP-dependent protein kinase from bovine heart exhibited significant interactions with nuclei from rat mammary tumors. These enzyme-nuclear interactions occurred when the intact holoenzyme was preincubated with 10 nM cAMP, 1 mM MgATP at 24 degrees C to produce high-affinity monophasic cAMP-dissociation kinetics. The enzyme-nuclear interactions are correlated with the loss of cAMP and PO4 from the purified enzyme. The data indicate that the high affinity cAMP-dependent protein kinase II exhibits significant nuclear interaction which may be related to cAMP function in rat mammary tumors.</p>\",\"PeriodicalId\":75821,\"journal\":{\"name\":\"Endocrine research communications\",\"volume\":\"9 3-4\",\"pages\":\"155-68\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3109/07435808209045761\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Endocrine research communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/07435808209045761\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine research communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/07435808209045761","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
牛心脏camp依赖性蛋白激酶与大鼠乳腺肿瘤细胞核表现出显著的相互作用。当完整的全酶与10 nM cAMP, 1 mM MgATP在24℃下预孵育时，这些酶核相互作用发生，产生高亲和力的单相cAMP解离动力学。酶核相互作用与纯化酶中cAMP和PO4的损失有关。这些数据表明，高亲和力的cAMP依赖性蛋白激酶II在大鼠乳腺肿瘤中表现出显著的核相互作用，这可能与cAMP功能有关。
cAMP-dependent protein kinase II interactions with nuclei derived from rat mammary tumor.
cAMP-dependent protein kinase from bovine heart exhibited significant interactions with nuclei from rat mammary tumors. These enzyme-nuclear interactions occurred when the intact holoenzyme was preincubated with 10 nM cAMP, 1 mM MgATP at 24 degrees C to produce high-affinity monophasic cAMP-dissociation kinetics. The enzyme-nuclear interactions are correlated with the loss of cAMP and PO4 from the purified enzyme. The data indicate that the high affinity cAMP-dependent protein kinase II exhibits significant nuclear interaction which may be related to cAMP function in rat mammary tumors.
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