A sensitive method for detection of circulating forms of human growth hormone by immunoautoradiography.

D A Cohen, K O'Connell, S L Blethen, W H Daughaday
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Abstract

Several different forms of hGH have been isolated from the pituitary, but little is known about modifications of synthesized forms that may exist in the circulation. We used a combination of serum immunoextraction with gel electrophoresis and electrophoretic blotting to detect circulating hGH isohormones. Three different components were identified, with the predominant form being 22,000 MW isomer. The method is sensitive to nanogram quantities of hGH and is applicable to the screening of human serum for the presence of variants of hGH.

免疫放射自显影法检测人生长激素循环形式的灵敏方法。
已经从垂体中分离出几种不同形式的生长激素,但对可能存在于循环中的合成形式的修饰知之甚少。我们采用血清免疫提取、凝胶电泳和电泳印迹相结合的方法检测循环hGH等激素。确定了三种不同的组分,主要形式为22,000 MW异构体。该方法对hGH的纳克量敏感,适用于筛选hGH变异的人血清。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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