Endocrine research communications最新文献_第10页

Effects of cholera toxin on thyroid cyclic AMP-dependent protein kinase and ornithine decarboxylase activities. 霍乱毒素对甲状腺环amp依赖性蛋白激酶和鸟氨酸脱羧酶活性的影响。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909070885

Y Friedman, P Hladis, D Babiarz-Crowell, G Burke

{"title":"Effects of cholera toxin on thyroid cyclic AMP-dependent protein kinase and ornithine decarboxylase activities.","authors":"Y Friedman, P Hladis, D Babiarz-Crowell, G Burke","doi":"10.3109/07435807909070885","DOIUrl":"https://doi.org/10.3109/07435807909070885","url":null,"abstract":"Cholera toxin activated beef thyroid cyclic AMP-dependent protein kinase in a dose (0.2 to 8 microgram/ml)-related fashion. Thus, when beef thyroid slices were incubated with toxin (8 microgram/ml) for 90 minutes and then assayed for protein kinase, the activity ratio (i.e. -cyclic AMP/+cyclic AMP) increased from 0.32 +/- 0.02 to 0.77 +/- 0.06. The toxin (5 microgram/ml)-induced increase was abolished by inclusion of ganglioside GM1 in the incubation medium (I50, 0.7 microgram/ml), whereas, gangliosides GD1a and GT1 were without effect. In contrast, TSH-activated protein kinase was unaffected by ganglioside addition. Cholera toxin increased rat thyroid ornithine decarboxylase (ODC) activity in-vitro in a dose (0.1 to 10 microgram/ml)-related fashion [basal, 100 cf cholera toxin (10 microgram/ml), 1500 pmol 14CO2/g tissue/30 min]. The toxin (1 microgram/ml)- (but not TSH-) induced increase in ODC was abolished by inclusion of ganglioside Ga and GT1 were without effect. Cholera toxin stimulation of ODC was inhibited by indomethacin or iodide as are the stimulatory effects of TSH or dibutyryl cyclic AMP. These results demonstrate that although there are differences in the TSH and cholera toxin responses with respect to receptor (ganglioside) interaction, they nevertheless elicit similar intracellular responses in thyroid.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 1","pages":"71-92"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435807909070885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11441343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Molecular conformation of thyroid hormones: structure and binding interactions of thyroxine. 甲状腺激素的分子构象:甲状腺素的结构和结合相互作用。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061100

V Cody

引用次数: 4

Diurnal variation in uterine estrogen receptors in immature female rats--inhibition by arginine vasotocin. 未成熟雌性大鼠子宫雌激素受体的日变化——精氨酸缩宫素的抑制作用。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909061794

M K Vaughan, J Buchanan, D E Blask, R J Reiter, P J Sheridan

引用次数: 8

Plasma immunoreactive calcitonin in lung cancer. 血浆免疫反应性降钙素与肺癌。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061104

B A Roos, A W Lindall, S B Baylin, J O'Neil, A L Frelinger, R S Birnbaum, P W Lambert

{"title":"Plasma immunoreactive calcitonin in lung cancer.","authors":"B A Roos, A W Lindall, S B Baylin, J O'Neil, A L Frelinger, R S Birnbaum, P W Lambert","doi":"10.1080/07435807909061104","DOIUrl":"https://doi.org/10.1080/07435807909061104","url":null,"abstract":"We have measured plasma calcitonin in 135 untreated eucalemic men with lung cancer and a control/smoker population. Calcitonin levels were determined by radioimmunoassay and validated by immunoextraction. Plasma immunoreactive calcitonin moieties were purified by immunoadsorbent chromatography, treated with mercaptoethanol and urea, and characterized by gel filtration. Artifacts in human calcitonin radioimmunoassays of cancer-patient plasmas were detected by parallel plasma incubations in a salmon calcitonin radioimmunoassay system which does not detect human calcitonin and by immunoprecipitation of tracer at the end of radioimmunoassay incubations. Heating fresh plasmas to 65 degrees C for 1.5 hours reduced radioimmunoassay artifacts without loss of calcitonin moieties. Such characterization of hypercalcitoninemia in each of the histopathological types of lung cancer has raised some important questions about the interpretation of plasma calcitonin radioimmunoassay measurements in lung cancer. Based on inhibition of tracer-antibody binding, plasma calcitonin seemed to be elevated in 18% (14/80) of basal plasma samples obtained from patients with epidermoid or with anaplastic lung cancer. Unequivocal hypercalcitoninemia (heat stable, causing no inhibition of antibody-tracer binding in the salmon calcitonin radioimmunoassays, and immunoextractable with human calcitonin antibodies) was not found in any of the apparently hypercalcitoninemic plasmas from persons with epidermoid or anaplastic lung cancer. By contrast, unequivocal hypercalcitoninemia was found in 27% (15/55) of plasmas from patients with small cell carcinoma or adenocarcinoma. Most of the immunoreactive calcitonin recovered from small cell and adenocarcinoma lung cancer plasmas with unequivocally elevated calcitonin is much larger than calcitonin monomer.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 2","pages":"169-90"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435807909061104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11443910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Parathyroid hormone release is not associated with acute sympathetic arousal in goats. 山羊甲状旁腺激素释放与急性交感神经觉醒无关。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909070884

P R Marques, D D Williams, L Shen, D G Johnson, D J Baylink, C C Gale

{"title":"Parathyroid hormone release is not associated with acute sympathetic arousal in goats.","authors":"P R Marques, D D Williams, L Shen, D G Johnson, D J Baylink, C C Gale","doi":"10.3109/07435807909070884","DOIUrl":"https://doi.org/10.3109/07435807909070884","url":null,"abstract":"Physiological activation of the sympathetic adrenomedullary (SAM) axis following both thermal and non thermal stress was assessed by changes in serum norepinephrine, glucose and/or protein as well as indices of peripheral blood flow. The occurrence of elevated serum parathyroid hormone (PTH) did not reliably reflect SAM activation as might be predicted from pharmacological studies that document a beta adrenergic receptor mechanism in the parathyroid gland that mediates catecholamine stimulated release of PTH into the circulation. The beta agonist isoproterenol at 1 microgram/min for 60 min did produce a transient increase in serum PTH at 20 min. Overall, the data raise doubts about the physiological significance of the adrenergic receptor in the parathyroid gland. Significant increases in serum PTH of 67% and 109% above basal respectively were seen following ruminal loading with cold and thermoneutral water. Associated with the PTH change were increased serum phosphorus and elevated or constant serum protein and serum total calcium.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 1","pages":"57-70"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435807909070884","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11692624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Radioimmunoassay for partially degraded thyrotropin. 部分降解促甲状腺素的放射免疫测定。

Endocrine research communications Pub Date : 1978-01-01 DOI: 10.3109/07435807809089013

B C Nisula, P N Fredlund

引用次数: 0

Pineal serotonin N-acetyltransferase activity and melatonin concentrations in prepubertal and adult Syrian hamsters exposed to short daily photoperiods. 暴露于短日光照周期的青春期前和成年叙利亚仓鼠的松果体血清素n -乙酰转移酶活性和褪黑激素浓度。

Endocrine research communications Pub Date : 1978-01-01 DOI: 10.1080/07435807809061095

E S Panke, R J Reiter, M D Rollag, T W Panke

{"title":"Pineal serotonin N-acetyltransferase activity and melatonin concentrations in prepubertal and adult Syrian hamsters exposed to short daily photoperiods.","authors":"E S Panke, R J Reiter, M D Rollag, T W Panke","doi":"10.1080/07435807809061095","DOIUrl":"https://doi.org/10.1080/07435807809061095","url":null,"abstract":"Pineal serotonin N-acetyltransferase activity (NAT) and melatonin concentrations were determined at various intervals in prepubertal (35 days old) and adult male hamsters (74 day old) throughout a 24 hour period with the animals kept in a light:dark cycle of 6:18 (lights on at 0600 h and off at 1200 h). In prepubertal animals, daytime pineal NAT activity of 0.20-0.28 nmoles 14C-N-acetyltryptamine/pineal/hour was maintained for 8 hours after the initiation of darkness. Peak pineal NAT activity of 0.46 +/- 0.64 nmoles 14C-N-acetyltryptamine/pineal/hour occurred 13 hours after the onset of darkness and remained significantly elevated until 0400 h (p less than 0.001). Daytime pineal melatonin concentrations of 78-194 pg/pineal gland also were maintained for 8 hours after the initiation of darkness. At 13 hours into the dark period, pineal melatonin concentrations rose to 788 +/- 150 pg/pineal gland (p less than 0.01 vs all other time points except 0230 h and 0400 h). At one hour before the onset of light both the pineal NAT activity and pineal melatonin concentrations returned to daytime values. Adult male hamsters had diurnal pineal NAT and melatonin rhythms which are indistinguishable from those found in the prepubertal animals.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"5 4","pages":"311-24"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435807809061095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11953023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Corticosteroid response of rabbits and rats to exogenous ACTH. 兔和大鼠对外源性促肾上腺皮质激素的皮质类固醇反应。

Endocrine research communications Pub Date : 1978-01-01 DOI: 10.1080/07435807809073633

C Boiti, R S Yalow

引用次数: 9

The effect of quipazine, a serotonin receptor agonist, on serum corticosterone concentration in rats. 羟色胺受体激动剂喹帕嗪对大鼠血清皮质酮浓度的影响。

Endocrine research communications Pub Date : 1978-01-01 DOI: 10.3109/07435807809089015

R W Fuller, H D Snoddy, J A Clemens

引用次数: 40

Studies on pituitary follitropin. IV. A conformation specific radioimmunoassay for the ovine hormone. 垂体促卵泡素的研究。羊激素的构象特异性放射免疫测定。

Endocrine research communications Pub Date : 1978-01-01 DOI: 10.1080/07435807809061093

M R Sairam

引用次数: 12