Endocrine research communications最新文献_第9页

Agonist (ovulation induction) and post-coital contraceptive properties of [D-Ala6] and [D-Trp6]-LHRH series. [D-Ala6]和[D-Trp6]-LHRH系列的激动剂(促排卵)和性交后避孕性能。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909070880

A Corbin, F J Bex, J P Yardley, R W Rees, T J Foell, D Sarantakis

引用次数: 11

Diurnal rhythm of pituitary-thyroid axis in male rats and the effect of adrenalectomy. 雄性大鼠垂体-甲状腺轴的昼夜节律及肾上腺切除术的影响。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909070883

S Ooka-Souda, D J Draves, P S Timiras

{"title":"Diurnal rhythm of pituitary-thyroid axis in male rats and the effect of adrenalectomy.","authors":"S Ooka-Souda, D J Draves, P S Timiras","doi":"10.3109/07435807909070883","DOIUrl":"https://doi.org/10.3109/07435807909070883","url":null,"abstract":"Levels of TSH, thyroxine (T4) and triiodothyronine (T3) were measured by radioimmunoassay in plasma (TSH, T4, T3) and pituitary (TSH) of 60-day old male Long-Evans rats. Definite diurnal rhythms were demonstrated in pituitary TSH, plasma TSH and T3 in intact rats, evidenced by the statistically significant differences between zenith and nadir for pituitary TSH, plasma TSH and T3. The zenith value of pituitary TSH and the nadir values of plasma TSH, T4 and T3 were observed at the same time (2400 h) as were the nadir value of pituitary TSH and the zenith values of plasma TSH, T4, and T3 (at 1200 h). Our results indicate that the rhythmicity of pituitary TSH content is a mirror image of that of plasma TSH. Adrenalectomy not only reduced plasma corticosterone levels to almost zero, but also decreased plasma T3 and T4 levels. In adrenalectomized rats, the absolute concentration of pituitary and plasma TSH increased at 1200 h and at 2400 h and, in both cases, the difference between values at 1200 h and at 2400 h persisted. The differences in plasma TSH, T3 and T4 between 1200 h and 2400 h were also observed in sham-adrenalectomized rats. These results suggest glucocorticoids do influence the pituitary-thyroid axis, but that the rhythmicity of the pituitary-thyroid axis does not seem to depend on the rhythmicity of glucocorticoid secretion.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 1","pages":"43-56"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435807909070883","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11777247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Radioimmunoassay of ovine and bovine serum progesterone without extraction and chromatography. 羊和牛血清黄体酮的放射免疫分析。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061106

B D Schanbacher

{"title":"Radioimmunoassay of ovine and bovine serum progesterone without extraction and chromatography.","authors":"B D Schanbacher","doi":"10.1080/07435807909061106","DOIUrl":"https://doi.org/10.1080/07435807909061106","url":null,"abstract":"A procedure for the radioimmunoassay of ovine and bovine serum progesterone is described which does not require extraction and chromatography. Serum samples are assayed directly, and a highly specific antiserum that was prepared in rabbits against 11 alpha-hydroxyprogesterone conjugated to bovine serum albumin is used. Interference from serum binding proteins is alleviated by use of a phosphate buffer containing 5% BSA and separation of bound and free tritiated progesterone by a double antibody procedure. Serum samples are assayed in a mini-vial, the bound fraction (double antibody precipitate) is mixed with scintillation solution and the radioactivity is counted in the same vial. The assay procedure is sensitive (10 pg, 100 pg/ml) and has acceptable accuracy and precision. Because there is no extraction or chromatography, serum progesterone is not lost. Most important, the procedure is specific for progesterone and measures serum progesterone concentrations in the ewe and cow which are comparable with those obtained with conventional assay techniques. The progesterone assay described herein provides a rapid, economical procedure that can facilitate the study of ovarian cyclicity and aid in the early diagnosis of pregnancy.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 4","pages":"265-77"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/07435807909061106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11748161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

A sensitive, convenient radioimmunoassay procedure which demonstrates that serum hTSH is suppressed below the normal range in thyrotoxic patients. 一个敏感的，方便的放射免疫测定程序，证明血清hTSH被抑制在正常范围以下甲状腺中毒患者。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909061799

R E Wehmann, H A Rubenstein, B C Nisula

引用次数: 33

The follicular iodide pool as a two-compartment system: evidence from the unstimulated thyroid gland. 卵泡碘池是一个双室系统:来自未受刺激的甲状腺的证据。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061102

C Simon, P Bastiani

引用次数: 0

Reconstitution of the solubilized insulin receptor in phospholipid vesicles. 磷脂囊泡中溶解胰岛素受体的重构。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061107

R J Gould, B H Ginsberg, A A Spector

引用次数: 14

Evaluation of in vitro incubation systems for the study of gonadotropin release. 促性腺激素释放研究体外培养系统的评价。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909070881

J L O'Conner, M B Allen, V B Mahesh

{"title":"Evaluation of in vitro incubation systems for the study of gonadotropin release.","authors":"J L O'Conner, M B Allen, V B Mahesh","doi":"10.3109/07435807909070881","DOIUrl":"https://doi.org/10.3109/07435807909070881","url":null,"abstract":"A detailed study was undertaken in order to determine if a pituitary-half incubation system were a suitable model for the study of anterior pituitary response to estradiol and LHRH. Considerable variation in the gonadotropin content of randomized pituitary halves was observed. Much less variation was found in matched halves. During the initial thirty minutes incubation of pituitary halves, a large spontaneous release of gonadotropins was observed. Time course secretion studies indicated that by four hours incubation, in the presence of 50 ng/ml LHRH, cumulative secretion of LH and FSH had far exceeded that of controls. Elevations in both cumulative secretion and rate of secretion were evident within 15-30 minutes of incubation. Regardless of LHRH dose, only 2-4% of either gonadotropin was secreted. Estradiol in the range of 10, 100, 500, 1,000 and 50,000 pg/ml had no significant effect on pituitary response to LHRH or on basal release, tissue levels or total gonadotropin. Based on these results, it was concluded that while the pituitary-half incubation system may be suitable for studying LHRH induced gonadotropin secretion, it is apparently of insufficient sensitivity to allow the collection of meaningful data concerning the effects of estradiol alone on gonadotropin secretion or estradiol modulation of LHRH induced gonadotropin secretion.","PeriodicalId":75821,"journal":{"name":"Endocrine research communications","volume":"6 1","pages":"15-36"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/07435807909070881","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11591944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

In vivo patterns of circulating steroids in adult male rats III. Effect of total parenteral nutrition on the diurnal variation of testosterone. 成年雄性大鼠体内类固醇循环模式III。全肠外营养对睾酮日变化的影响。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061098

R J Keating, R K Tcholakian

引用次数: 2

Molecular conformation of thyroid hormones: structure and binding interactions of thyroxine. 甲状腺激素的分子构象:甲状腺素的结构和结合相互作用。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.1080/07435807909061100

V Cody

引用次数: 4

Diurnal variation in uterine estrogen receptors in immature female rats--inhibition by arginine vasotocin. 未成熟雌性大鼠子宫雌激素受体的日变化——精氨酸缩宫素的抑制作用。

Endocrine research communications Pub Date : 1979-01-01 DOI: 10.3109/07435807909061794

M K Vaughan, J Buchanan, D E Blask, R J Reiter, P J Sheridan

引用次数: 8