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Purification and release from quiescence of umbilical cord blood early progenitors reveal their potential to engraft adults. 脐带血早期祖细胞的纯化和休眠释放揭示了其移植成体的潜力。
Blood cells Pub Date : 1994-01-01
J Hatzfeld, P Batard, A A Cardoso, M L Li, B Panterne, P Sansilvestri, M Ginsbourg, J P Levesque, A Hatzfeld
{"title":"Purification and release from quiescence of umbilical cord blood early progenitors reveal their potential to engraft adults.","authors":"J Hatzfeld,&nbsp;P Batard,&nbsp;A A Cardoso,&nbsp;M L Li,&nbsp;B Panterne,&nbsp;P Sansilvestri,&nbsp;M Ginsbourg,&nbsp;J P Levesque,&nbsp;A Hatzfeld","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Steel factor (SF) increases the frequency of colony formation by CD34+ CD38- cycling cells, but it does not reverse the effect of an autocrine production of transforming growth factor (TGF)-beta 1 by early progenitors of the stem cell compartment. We have used optimal culture conditions supplemented with SF and anti-TGF-beta serum to estimate the proliferative capacity and ability to generate early progenitors in long-term cultures of bone marrow and umbilical cord blood cells. We estimate that the CD34+ CD38- cells from a typical umbilical cord blood sample produce equivalent numbers of granulocyte erythrocyte macrophage megakaryocyte colony-forming units (CFU), twice as many granulocyte-macrophage (GM) CFU, and three times as many erythroid burst-forming units as the same population from an average bone marrow sample used in adult transplantation. These results suggest that umbilical cord blood is a suitable source of cells for adult transplantation.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"430-4; discussion 434-5"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18544573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytokine regulation of early B-lymphopoiesis assessed in culture. 细胞因子对早期b淋巴生成的调节作用。
Blood cells Pub Date : 1994-01-01
F Hirayama, M Ogawa
{"title":"Cytokine regulation of early B-lymphopoiesis assessed in culture.","authors":"F Hirayama,&nbsp;M Ogawa","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lymphohemopoietic progenitors that are capable of expressing B-cell and myeloid lineages may be cultured from bone marrow cells of adult mice by using a two-step methylcellulose culture system. In this system, the primary colonies expressing myeloid lineages are plated in secondary culture for B-lymphoid colony formation. We have observed that combinations of two factors based on steel factor (SLF), such as SLF plus interleukin (IL)-6, SLF plus granulocyte colony-stimulating factor (G-CSF), and SLF plus IL-11 support the differentiation and proliferation of B-cell progenitors from the lymphohemopoietic progenitors. Surprisingly, IL-3 failed to support B lymphopoiesis either alone or in combination with other factors. In addition, when added to permissive culture conditions, IL-3 and IL-1 independently inhibited the B-cell potential of the primary colonies. The inhibitory effects of IL-3 and IL-1 observed in this in vitro system may be significant in the selection of cytokine combinations for in vitro expansion of hemopoietic stem cells.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"341-6; discussion 346-7"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18546348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone marrow culture and transduction of stem cells in a miniature swine model. 微型猪模型的骨髓培养和干细胞转导。
Blood cells Pub Date : 1994-01-01
D W Emery, T Sablinski, J S Arn, C LeGuern, D H Sachs
{"title":"Bone marrow culture and transduction of stem cells in a miniature swine model.","authors":"D W Emery,&nbsp;T Sablinski,&nbsp;J S Arn,&nbsp;C LeGuern,&nbsp;D H Sachs","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recombinant retroviral vectors, engineered to express the beta-chain gene of swine major histocompatibility complex class II DR, were developed for the genetic modification of swine hematopoietic stem cells (HSC). The expression of these vectors in swine bone marrow has been studied both in culture and after bone marrow transplantation. In addition, myeloid progenitor colony assays were performed on swine umbilical cord blood as part of a study to identify alternative sources of HSC for somatic gene transfer, revealing the presence of both granulocyte macrophage colony forming-units (CFU-GM) and CFU-Mix at frequencies comparable to those found in juvenile swine bone marrow.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"498-502; discussion 502-3"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18749581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Commentary: results of umbilical cord transplantation. 评论:脐带移植的结果。
Blood cells Pub Date : 1994-01-01
P G Beatty
{"title":"Commentary: results of umbilical cord transplantation.","authors":"P G Beatty","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"242-4"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18750391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ex vivo expansion of cord blood-derived stem cells and progenitors. 脐带血来源的干细胞和祖细胞的体外扩增。
Blood cells Pub Date : 1994-01-01
M A Moore, I Hoskins
{"title":"Ex vivo expansion of cord blood-derived stem cells and progenitors.","authors":"M A Moore,&nbsp;I Hoskins","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cord blood-derived CD34+ cells have proved superior to adult marrow or elicited peripheral blood in ex vivo cell and progenitor expansion. In addition, cord blood-derived cells with a phenotype and function of early stem cells (e.g., long-term culture-initiating cells) can be expanded in vitro in the presence of a combination of cytokines by 15- to 20-fold over 7-14 days. These observations suggest that ex vivo expansion of cord blood CD34+ cells will permit improved engraftment of adults and will prove effective in retroviral-mediated gene transfection of early stem cell populations.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"468-79; discussion 479-81"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18544576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cord blood transplantation in thalassemia. 脐带血移植治疗地中海贫血。
Blood cells Pub Date : 1994-01-01
S Issaragrisil
{"title":"Cord blood transplantation in thalassemia.","authors":"S Issaragrisil","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thalassemias and hemoglobinopathies are prevalent in Thailand. Bone marrow transplantation can cure thalassemia, but less than 30% of the patients have an HLA-identical sibling. Cord blood is an alternative source of stem cells for transplantation. By prenatal diagnosis, the fetus can be diagnosed as having thalassemic disease. DNA typing for HLA will be performed. Cord blood can be collected for transplantation if the fetus is not affected and is HLA-identical to the patient. We report a successful cord blood transplantation in a patient with beta-thalassemia/hemoglobin E disease.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"259-62; discussion 263"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18751660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Commentary: cytokine support after cord blood transplantation. 评论:脐带血移植后的细胞因子支持。
Blood cells Pub Date : 1994-01-01
J J Nemunaitis
{"title":"Commentary: cytokine support after cord blood transplantation.","authors":"J J Nemunaitis","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"264-6"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18751661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Directed cell movement: a biophysical analysis. 定向细胞运动:生物物理分析。
Blood cells Pub Date : 1993-01-01
H Gruler
{"title":"Directed cell movement: a biophysical analysis.","authors":"H Gruler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The directed movement or directed growth of a cell in a polar guiding field (such as an electric field, a concentration gradient of chemotactic active molecules, a necrotactic gradient induced by a lysed cell, etc.) can be characterized by two independent variables: the speed, nu, of the cell and its migration angle, phi. Here it is shown that the direction of migration is controlled by a cellular automatic controller. The automatic controller can be regarded as the framework for the directed movement or growth and it can be applied even when the physicochemical signals to which the cell is responding are unknown.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 1","pages":"91-110; discussion 110-3"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19384658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Long-term chemotaxis of neutrophils in stable gradients: preliminary evidence of periodic behavior. 中性粒细胞在稳定梯度下的长期趋化:周期性行为的初步证据。
Blood cells Pub Date : 1993-01-01
G A Dunn, D Zicha
{"title":"Long-term chemotaxis of neutrophils in stable gradients: preliminary evidence of periodic behavior.","authors":"G A Dunn,&nbsp;D Zicha","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We observed and recorded the behavior of human neutrophils in stable linear gradients of the chemotactic attractant f-Met-Leu-Phe in a recently developed, direct-viewing, chemotaxis chamber. The cells were kept cold for the first hour after setting up the chemotaxis chamber in order to establish a stable gradient before allowing the cells to spread and begin to move by warming them up to 37 degrees C. We found that the cells showed very significant chemotaxis at different times throughout the 2-hour period of observation but, unexpectedly, the activity occurred in periodic or quasi-periodic cycles during which the cells entered phases of undirected motility lasting for several minutes. We discuss whether the periodicity is a population or single cell-based phenomenon and briefly speculate on its possible analogy to the well-known periodic chemotaxis of slime mould amoebas.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 1","pages":"25-39; discussion 39-41"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19386042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CD23 and chronic lymphocytic leukemia. CD23与慢性淋巴细胞白血病。
Blood cells Pub Date : 1993-01-01
M Sarfati
{"title":"CD23 and chronic lymphocytic leukemia.","authors":"M Sarfati","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Chronic lymphocytic leukemia (CLL) is classically defined by the proliferation and accumulation of monoclonal CD5 positive B cells \"arrested\" at an intermediate stage of differentiation. The CD23 antigen (the low affinity receptor for IgE (Fc epsilon RII) is a 45 kD membrane glycoprotein primarily expressed on surface IgM and IgD positive B cells. The aim of this paper is to establish the link between CD23 and CLL disease and to propose that the CD23 molecule is not simply a marker of CLL disease but may also be involved in the proliferation of the leukemic B cells.</p>","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 3","pages":"591-6; discussion 597-9"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19014750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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