Blood cellsPub Date : 2026-06-16DOI: 10.3390/cells15121095
Yi Yang, Shengxiang Guo, Wu Luo, D Liu, Xincong Kang
{"title":"Honokiol Ameliorates Hepatic Lipid Accumulation by Deacetylating PPARG via SIRT3","authors":"Yi Yang, Shengxiang Guo, Wu Luo, D Liu, Xincong Kang","doi":"10.3390/cells15121095","DOIUrl":"https://doi.org/10.3390/cells15121095","url":null,"abstract":"Dysregulated lipid metabolism is a core pathogenic driver of type 2 diabetes. Honokiol (HKL), the major bioactive constituent of Magnolia officinalis, possesses anti-diabetic and lipid-regulatory properties. However, the underlying molecular mechanism remains elusive. This study investigates how HKL ameliorates high-glucose/high-fat (HGHF)-induced hepatic lipid accumulation, with a focus on the role of SIRT3-mediated deacetylation of peroxisome proliferator-activated receptor γ (PPARG). The core targets of HKL were identified through network pharmacology and molecular docking. Human hepatic MIHA cells were treated with glucose (Glu, 40 mM) and palmitic acid (0.2~0.3 mM PA) to establish a lipid accumulation model, followed by treatment with HKL (5–10 μM) with or without a confirmed selective SIRT3 inhibitor 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP). Lipid accumulation was assessed by Oil Red O staining and by measuring triglyceride (TG) and total cholesterol (TC) levels. Protein expression and the SIRT3-PPARG interaction were analyzed by Western blot and co-immunoprecipitation (Co-IP). SIRT3 and PPARG were identified as core targets of HKL, exhibiting strong binding with calculated energies of −6.834 and −6.579 kcal/mol, respectively. In MIHA cells, HGHF (40 mM Glu + 0.2–0.3 mM PA) induced lipid accumulation, including increased lipid droplets, and elevated TG (2.5–3.2-fold) and TC (2.2–2.8-fold) contents in a dose-dependent manner, accompanied by downregulated SIRT3/PPARG expression and heightened global protein acetylation. The non-cytotoxic HGHF-M condition (40 mM Glu + 0.2 mM PA) was selected for further experiments. HKL (5–10 μM) dose-dependently reduced lipid accumulation by ~38–60%, decreased TG and TC levels by up to ~13% and ~30%, and restored SIRT3/PPARG expression. The protective effects of HKL were reversed by inhibition of SIRT3 with 3-TYP. Co-IP confirmed the interaction between SIRT3 and PPARG, and SIRT3 overexpression significantly decreased the acetylation level of PPARG. This study suggests that HKL ameliorates hepatic lipid accumulation via SIRT3-mediated deacetylation of PPARG, providing an experimental basis for considering HKL as a potential therapeutic agent against metabolic disorders.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"15 12","pages":"1095-1095"},"PeriodicalIF":0.0,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2073-4409/15/12/1095/pdf?version=1781602201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blood cellsPub Date : 2026-06-11DOI: 10.3390/cells15121070
Zhongwei Zhang, Huiran Li, 史忠岚, Xuan Bai, Peipei Yin, Lingguang Yang
{"title":"Structure-Based Virtual Screening and Mechanistic Characterization of Methotrexate and Selinexor as Potent Anti-Melanogenic Agents via Multi-Pathway Suppression of MITF","authors":"Zhongwei Zhang, Huiran Li, 史忠岚, Xuan Bai, Peipei Yin, Lingguang Yang","doi":"10.3390/cells15121070","DOIUrl":"https://doi.org/10.3390/cells15121070","url":null,"abstract":"Tyrosinase is a pivotal therapeutic target for hyperpigmentation disorders, yet current inhibitors frequently exhibit limited potency and suboptimal safety. Here, we employed structure-based virtual screening of an FDA-approved drug library against a refined human tyrosinase homology model, identifying methotrexate and selinexor as potent anti-melanogenic candidates. Both compounds markedly suppressed cellular tyrosinase activity and melanin synthesis (IC50 < 1 µM) in MNT-1 melanoma cells. Mechanistically, they orchestrate a multi-pronged downregulation of microphthalmia-associated transcription factor (MITF) by attenuating cAMP/PKA/CREB signaling, promoting β-catenin degradation, and accelerating MITF proteolysis via AKT/ERK activation. Additionally, they bolster the intracellular antioxidant defense system. These findings unveil a sophisticated regulatory network and suggest that with strict control of systemic exposure through optimized topical formulations, these FDA-approved agents could be further investigated as potential localized treatments for pigmentary disorders.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"15 12","pages":"1070-1070"},"PeriodicalIF":0.0,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blood cellsPub Date : 2014-11-14DOI: 10.1002/9781118817322.CH9
B. Bain
{"title":"Disorders of white cells","authors":"B. Bain","doi":"10.1002/9781118817322.CH9","DOIUrl":"https://doi.org/10.1002/9781118817322.CH9","url":null,"abstract":"","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"207 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9781118817322.CH9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50730325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Blood cellsPub Date : 2014-11-14DOI: 10.1002/9781118817322.CH1
B. Bain
{"title":"Blood sampling and blood film preparation and examination","authors":"B. Bain","doi":"10.1002/9781118817322.CH1","DOIUrl":"https://doi.org/10.1002/9781118817322.CH1","url":null,"abstract":"Performing an accurate blood count and correctly interpreting a blood film require that an appropriate sample from the patient, mixed with the correct amount of a suitable anticoagulant, is delivered to the laboratory without undue delay. No artefacts should be introduced during these procedures. The identity of the patient requiring blood sampling should be carefully checked before performing a venepuncture. This is usually done by requesting the patient to state surname, given name and date of birth and, for hospital inpatients, by checking a wristband to verify these details and, in addition, the hospital number. To reduce the chance of human error, bottles should not be labelled in advance. The person performing the phlebotomy must conform to local guidelines, including those for patient identification. Although traditionally more attention has been given to patient identification in relation to blood transfusion, it should be noted that wrong treatment has also followed the misidentification of patients from whom samples are taken for a blood count and identification must also be taken seriously in this field. More secure identification of inpatients can be achieved by the use of electronic devices in which the patient’s identity is scanned in from a bar‐ coded wristband by means of a hand‐held device. Patients should either sit or lie comfortably and should be reassured that the procedure causes only minimal discomfort; they should not be told that venepuncture is painless, since this is not so. It is preferable for apprehensive patients to lie down. Chairs used for venepuncture should preferably have adjustable armrests so that the arm can be carefully positioned. Armrests also help to ensure patient safety, since they make it harder for a fainting patient to fall from the chair. I have personally observed one patient who sustained a skull fracture when he fainted at the end of a venepuncture and fell forward onto a hard floor, and two other patients, neither previously known to be epileptic, who suffered epileptiform convulsions during venepuncture. Such seizures may not be true epilepsy, but consequent on hypoxia following brief vagal‐induced cessation of heart beat [1]. If venepunctures are being performed on children or on patients unable to cooperate fully, then the arm for venepuncture should be gently but firmly immobilized by an assistant. Gloves should be worn during venepuncture, for the protection of the person carrying out the procedure. Non‐latex gloves must be available if either the phlebotomist or the patient is allergic to latex. The needle to enter the patient must not be touched, so that it remains sterile. In some circumstances, the patient should rest prior to venesection. In endurance athletes being tested for a ‘biological passport’, 10 minutes’ rest in a seated position has been found to be sufficient for the haemoglobin concentration (Hb) and haematocrit (Hct) to fall to a stable level [2].","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"322 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9781118817322.CH1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50729550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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