Blood cells最新文献_第10页

The cellular and molecular environment in leukemia. 白血病的细胞和分子环境。

Blood cells Pub Date : 1993-01-01

L Sachs

引用次数: 0

Human leukemia: re-examination of dogmas. 人类白血病:对教条的重新审视。

Blood cells Pub Date : 1993-01-01

A Epstein

引用次数: 0

The history of leukemia: a personal perspective. 白血病的历史:个人视角。

Blood cells Pub Date : 1993-01-01

G Piller

引用次数: 0

Problems existing in differentiation therapy of acute promyelocytic leukemia (APL) with all-trans retinoic acid (ATRA). 全反式维甲酸(ATRA)分化治疗急性早幼粒细胞白血病(APL)存在的问题。

Blood cells Pub Date : 1993-01-01

Z Y Wang, Z Chen, W Huang, X S Li, J X Lu, L A Huang, F Q Zhang, L J Gu, R R Ouyang, S J Chen

{"title":"Problems existing in differentiation therapy of acute promyelocytic leukemia (APL) with all-trans retinoic acid (ATRA).","authors":"Z Y Wang, Z Chen, W Huang, X S Li, J X Lu, L A Huang, F Q Zhang, L J Gu, R R Ouyang, S J Chen","doi":"","DOIUrl":"","url":null,"abstract":"A large number of acute promyelocytic leukemia (APL) patients, treated with all-trans retinoic acid (ATRA) and chemotherapy, were studied. The results of the studies are as follows: (1) Among 65 patients investigated for the postremissional therapy, the 5-year survival probabilities were 0.20 +/- 0.13 (mean +/- SE) in the group treated with ATRA alone, 0.47 + 0.10 (mean +/- SE) in the group using chemotherapy alone and 0.42 +/- 0.09 (mean +/- SE) in the group treated with chemotherapy and ATRA. (2) The main severe adverse effects in the ATRA treatment include retinoic acid syndrome, renal failure, and thrombosis. These sequelae were observed more frequently in cases with persistent, marked elevation of white blood cell count without significant maturation of leukemic promyelocytes. (3) APL is not a homogeneous disease in that among 50 patients studied at the molecular level, although a PML-RARA fusion gene was detected in 45 cases, one had a variant translocation t(11;17) bearing fusion gene PLZF-RARA, one presented no obvious structural alteration of the PML gene while the RARA gene was rearranged, and three patients had no rearrangement of either PML or RARA genes. (4) Using RT/PCR to detect minimal residual disease, we found positive rates of 22%, 18.4%, and 11.5%, respectively, 12, 24, and 36 months after CR. This observation justifies the use of chemotherapy for at least 3 years after CR induced by ATRA. (5) It seems likely that the fusion gene PML-RARA plays an important role in APL leukemogenesis and in its response to the ATRA treatment.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 3","pages":"633-41; discussion 642-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19014753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of multiple kinases in neutrophil activation. 中性粒细胞活化中多种激酶的参与。

Blood cells Pub Date : 1993-01-01

D J Lu, W Furuya, S Grinstein

引用次数: 0

Inhibition of sickling after reduction of intracellular hemoglobin concentration with an osmotic pulse: characterization of the density and hemoglobin concentration distributions. 渗透脉冲降低细胞内血红蛋白浓度后对镰状细胞的抑制作用:密度和血红蛋白浓度分布的表征。

Blood cells Pub Date : 1993-01-01

R S Franco, R Barker-Gear, R Green

{"title":"Inhibition of sickling after reduction of intracellular hemoglobin concentration with an osmotic pulse: characterization of the density and hemoglobin concentration distributions.","authors":"R S Franco, R Barker-Gear, R Green","doi":"","DOIUrl":"","url":null,"abstract":"Hemoglobin S polymerization is markedly dependent on intracellular hemoglobin concentration. In the studies presented here, sickle RBC were subjected to a transient osmotic stress, which induced a short period of increased membrane permeability and allowed partial efflux of Hb S. Morphological sickling of the resulting hypochromic RBC was inhibited. The response of RBC to this osmotic pulse is influenced by the presence of a polyanion, which in these experiments was either inositol hexaphosphate (IHP, 27 mM or 46 mM) or pyrophosphate (69 mM or 95 mM). The decrease in MCHC, measured manually, ranged from 3.1 +/- 1.7 (1 SD) to 6.3 +/- 2.8 g/dl, depending on the conditions used during modification. Parallel electronic analysis of RBC indices demonstrated a comparable decrease in MCHC which was due to both an increased MCV and a decreased MCH. Since the modified cell population is quite heterogeneous, cells were analyzed using discontinuous stractan gradients and/or a laser-based instrument which measures the hemoglobin concentration (HC) of individual cells. For most treatment conditions, the modified cells have a bimodal HC distribution with one peak centered at about 20 g/dL and the other peak corresponding to the unmodified cells. With the higher concentration of IHP, however, many cells had an intermediate HC. For modified RBC with a bimodal HC distribution (27 mM IHP, 69 mM PP, 95 mM PP), inhibition of morphological sickling was proportional to the change in HC and there were no subpopulations with an increased tendency to undergo sickling. However, the intermediate density cells present when RBCs were treated with the higher concentration of IHP underwent sickling at a higher oxygen partial pressure than control cells.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 2","pages":"475-88; discussion 489-91"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19299106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure and function of human and mouse Fc gamma RII. 人和小鼠Fc γ RII的结构和功能。

Blood cells Pub Date : 1993-01-01

R J Looney

引用次数: 0

Dynamic aspects of cytoskeletal protein distribution in T lymphocytes: involvement of calcium in spectrin reorganization. T淋巴细胞中细胞骨架蛋白分布的动态方面:钙参与谱蛋白重组。

Blood cells Pub Date : 1993-01-01

C C Gregorio, J D Black, E A Repasky

{"title":"Dynamic aspects of cytoskeletal protein distribution in T lymphocytes: involvement of calcium in spectrin reorganization.","authors":"C C Gregorio, J D Black, E A Repasky","doi":"","DOIUrl":"","url":null,"abstract":"Our studies on the lymphocyte cytoskeleton have revealed a significant heterogeneity in the subcellular distribution of lymphocyte spectrin in vivo. Two model systems have been characterized in which this protein exhibits dynamic properties in response to activation signals. In this study, we have investigated the role of calcium in the activation-induced reorganization of spectrin in one of these systems, the DO-11.10 T cell hybridoma. DO-11.10 cells, as well as several other in vitro T cell models, can homogeneously and constitutively express a distinct cytoplasmic aggregate of spectrin that is rapidly fragmented upon activation. The reversible dissipation of the aggregate of spectrin is accompanied by an increase in the levels of spectrin diffusely distributed throughout the cytoplasm and at the plasma membrane. Pretreatment of cells with calcium-free medium, or with medium containing ethyleneglycol-bis-(beta-aminoethyl ether)N,N'-tetraacetic acid (EGTA) or verapamil, significantly blocked the reorganization of spectrin induced by Concanavalin A or the calcium ionophore A23187, and also prevented the release of IL-2 from these cells. Further, immunofluorescent and ultrastructural analyses revealed abnormalities in the organization of spectrin induced by these treatments. These findings are discussed in light of our other studies, indicating a role for spectrin in early events associated with activation of T lymphocytes in vivo and in vitro.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 2","pages":"361-71; discussion 371-3"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19300501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil adhesion to endothelial cells. 中性粒细胞粘附内皮细胞。

Blood cells Pub Date : 1993-01-01

O Abbassi, T K Kishimoto, L V McIntire, C W Smith

{"title":"Neutrophil adhesion to endothelial cells.","authors":"O Abbassi, T K Kishimoto, L V McIntire, C W Smith","doi":"","DOIUrl":"","url":null,"abstract":"The emigration of neutrophils at sites of inflammation apparently requires intercellular adhesion. Initially, leukocyte adherence is observed in postcapillary venules where neutrophils roll along the luminal surface of the endothelial cells before stopping, changing shape, and migrating into the perivascular tissue. Recent evidence indicates that the adhesion molecules supporting the rolling phenomenon are distinct from those required for stopping and transmigration. The contribution of E-selectin (ELAM-1) to neutrophil adhesion and rolling was investigated using anti-E-selectin monoclonal antibody in an in vitro adhesion assay of isolated human neutrophils to murine L-cell monolayers stably transfected with human E-selectin cDNA. Isolated human neutrophils adhered to E-selectin expressing L-cell monolayers under physiological wall shear stress of 1.85 dynes/cm2 but not to untransfected L-cells or ICAM-1 expressing L-cells. 47.8 +/- 6.0% of these adherent cells were rolling at an average rolling velocity of 10.6 +/- 1.7 microns/second. This adhesion and rolling was almost completely blocked by anti-E-selectin monoclonal antibody. Monoclonal antibody to L-selectin also reduced adhesion to E-selectin expressing cells by 70%. Chemotactic stimulation of neutrophils reduced both the number of adherent and rolling cells and the average velocity of the rolling cells without influencing the percentage of attached cells that were rolling. Pretreatment with anti-CD18 monoclonal antibody did not reduce the adhesion of the activated neutrophils but reversed the reduction in velocity caused by activation of these cells. The inhibitory potential of the anti-E-selectin monoclonal antibody was much less pronounced in adhesion of isolated neutrophils to human umbilical vein endothelial cell monolayers under conditions of flow and was limited to one third of the total adhesion. The proportion of the adherent neutrophils which transmigrated to the subluminal space of the endothelial cell monolayers was independent of pretreatment with anti-E-selectin monoclonal antibody.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"19 2","pages":"245-59; discussion 259-60"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18518692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ion channels and receptor-mediated Ca2+ influx in neutrophil granulocytes. 中性粒细胞中离子通道和受体介导的Ca2+内流。

Blood cells Pub Date : 1993-01-01

K H Krause, N Demaurex, M Jaconi, D P Lew

引用次数: 0