Blood cells最新文献_第5页

Allogeneic responses of human umbilical cord blood. 人类脐带血的异体反应。

Blood cells Pub Date : 1994-01-01

G Risdon, J Gaddy, H E Broxmeyer

引用次数: 0

Use of granulocyte-macrophage colony-stimulating factor in two children treated with cord blood transplantation. 粒细胞-巨噬细胞集落刺激因子在儿童脐带血移植中的应用。

Blood cells Pub Date : 1994-01-01

M R Vowels, K Tiedemann, R Lam-Po-Tang, D P Tucker

{"title":"Use of granulocyte-macrophage colony-stimulating factor in two children treated with cord blood transplantation.","authors":"M R Vowels, K Tiedemann, R Lam-Po-Tang, D P Tucker","doi":"","DOIUrl":"","url":null,"abstract":"Cord blood contains stem cells in amounts similar to or slightly less than those present in a bone marrow collection to be used for bone marrow transplantation (BMT). Too few cord blood transplants (CBT) have yet been performed to define the ability to achieve engraftment and the rate of engraftment. Two cord blood transplants have been performed using granulocyte-macrophage colony stimulating factor (GM-CSF) to hasten engraftment. Two children, aged 5 and 6 years received a CBT using HLA-identical stem cells collected at the birth of a sibling. One child had X-linked lymphoproliferative disease (XLP), and the other, acute lymphoblastic leukemia in second complete remission. One had an ABO and one an Rh blood group mismatch. Conditioning therapy consisted of cyclophosphamide, melphalan, and antithymocyte globulin or busulphan and cyclophosphamide. Graft-versus-host disease prophylaxis was methotrexate and cyclosporine or cyclosporine. Both children were given GM-CSF at 5 micrograms/kg/day from day 1 until the absolute neutrophil count (ANC) reached 1.0 x 10(9)/L for 3 consecutive days. If this level was not reached by day 14, the dose of GM-CSF was doubled. Both children engrafted rapidly, with ANCs reaching 0.5 x 10(9)/L in 12 and 16 days. Engraftment was confirmed by blood group in both and sex chromosome typing in one. Both children developed mild GVHD localized to skin, which resolved with steroid therapy. The child with XLP was cured and has survived for 34 months; the second child has survived 27 months with normal marrow function but has had a relapse of leukemia.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"249-54; discussion 254-5"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18750393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary: hematologic rescue with umbilical cord blood progenitor cells. 评论:脐带血祖细胞的血液学抢救。

Blood cells Pub Date : 1994-01-01

J E Wagner

引用次数: 0

Enrichment for primitive hemopoietic progenitors of marrow cells from 5-fluorouracil-treated mice and normal mice. 5-氟尿嘧啶处理小鼠和正常小鼠骨髓细胞原始造血祖细胞的富集。

Blood cells Pub Date : 1994-01-01

M Ogawa, J P Shih, N Katayama

{"title":"Enrichment for primitive hemopoietic progenitors of marrow cells from 5-fluorouracil-treated mice and normal mice.","authors":"M Ogawa, J P Shih, N Katayama","doi":"","DOIUrl":"","url":null,"abstract":"Study of the mechanisms regulating stem cells would be significantly facilitated if a purified population of stem cells were available. During the last 4 years, our laboratory has been engaged in enrichment of murine marrow cells for primitive hemopoietic progenitors. We primarily used marrow cells from mice treated with 150 mg/kg of 5-fluorouracil (5-FU), and our assay for the primitive progenitors was formation of multilineage colonies supported by a combination of interleukin-3 (IL-3) and IL-6. First, we found that post-5-FU marrow cells with a density of 1.0631-1.0770 g/cm3, negative for lineage-specific markers and positive for Ly-6A/E are routinely enriched for multipotential progenitors by approximately 800-fold. We then observed that J11d.2 and c-kit are additional useful markers for further enrichment of the primitive hemopoietic progenitors. Cell cycle-dormant primitive progenitors are primarily in the J11d.2+ fraction, whereas more mature progenitors are J11d.2-. The primitive progenitors express relatively low levels of c-kit, while more mature, actively cycling progenitors express high levels of c-kit. Combinations of these markers may be useful in enrichment of marrow cells of normal mice for primitive hemopoietic progenitors.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 1","pages":"7-11; discussion 11-3"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18990677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adhesion molecules in hematopoietic cells. 造血细胞中的粘附分子。

Blood cells Pub Date : 1994-01-01

T Kinashi, T A Springer

引用次数: 0

Interaction of primitive human myeloid and lymphoid progenitors with the marrow microenvironment. 原始人类髓系和淋巴系祖细胞与骨髓微环境的相互作用。

Blood cells Pub Date : 1994-01-01

P McGlave, C Verfaillie, J Miller

{"title":"Interaction of primitive human myeloid and lymphoid progenitors with the marrow microenvironment.","authors":"P McGlave, C Verfaillie, J Miller","doi":"","DOIUrl":"","url":null,"abstract":"Primitive human hematopoietic progenitors containing a high proportion of long-term culture-initiating cells (LTCICs) are found in the 34+DR- fraction of bone marrow mononuclear cells (BMMNCs). These progenitors adhere selectively to the 33/66-kD binding domain of fibronectin and to the FN-CHII binding site, unlike more committed progenitors, which adhere less selectively to fibronectin components. These differences in adhesion to stromal components may explain selective homing and release of progenitors at varying levels of differentiation from the marrow compartment. In additional studies, we demonstrate that cultivation of primitive progenitors in a stroma noncontact long-term culture allows both differentiation of primitive progenitors and conservation of LTCICs. These observations (1) demonstrate that expansion of primitive progenitors does not require stromal contact, (2) shed light on the regulatory role of stroma in myeloid differentiation, and (3) suggest strategies for both ex vivo myeloid progenitor expansion and retrovirus gene insertion. Finally, we demonstrate that a natural killer cell population can be derived from primitive hematopoietic progenitors in a modified long-term culture model. Our findings suggest an important role for marrow stroma in lymphoid differentiation from primitive progenitors and in expression of CD2, a lymphoid marker ordinarily associated with passage of T-lymphocyte progenitors through the thymus.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 1","pages":"121-6; discussion 126-8"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18988868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene transfer into hematopoietic stem cells. 基因转移到造血干细胞。

Blood cells Pub Date : 1994-01-01

A W Nienhuis

{"title":"Gene transfer into hematopoietic stem cells.","authors":"A W Nienhuis","doi":"","DOIUrl":"","url":null,"abstract":"The ability to insert a gene into hematopoietic stem cells and achieve lineage specific expression of the transferred gene within hematopoietic organs following bone marrow transplantation would create the potential to effectively treat many genetic and acquired diseases. The use of retroviral vectors to achieve this purpose has been investigated extensively in animal models and most recently, in humans. In the murine model, about 20-30% of repopulating stem cells can be genetically modified with a retroviral vector. Peripheral blood stem cells, mobilized by cytokine administration in splenectomized animals, are readily transduced and are capable of long-term reconstitution of transplant recipients with genetically modified cells. Similar protocols have been utilized to transduce highly purified stem cells from rhesus monkeys. Although long-term repopulation with cells that persistently express the transferred gene has been achieved, the frequency of cells containing the vector genome is only about 1-2%. Genetic marking of human bone marrow and peripheral blood cells has been utilized to investigate their potential for contributing to long-term reconstitution following autologous transplantation. Future work will focus on improving gene transfer efficiencies for specific therapeutic applications.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 1","pages":"141-7; discussion 147-8"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18988869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo polymerization of sickle-cell hemoglobin: a theoretical study. 镰状细胞血红蛋白的体内聚合:理论研究。

Blood cells Pub Date : 1994-01-01

V B Makhijani, G R Cokelet

{"title":"In vivo polymerization of sickle-cell hemoglobin: a theoretical study.","authors":"V B Makhijani, G R Cokelet","doi":"","DOIUrl":"","url":null,"abstract":"Several studies on the gelation and oxygenation state of sickle red blood cells have been done under conditions of equilibrium. The kinetics of sickle hemoglobin (HbS) polymerization have also been studied extensively in fully deoxygenated HbS solutions. The issue of the relevance of these investigations to the physiological in vivo situation has not been addressed. Here, we use a theoretical model to compare theoretical equilibrium predictions of HbS polymer concentration and cellular oxygen content, previously validated against equilibrium data, with the corresponding values under physiologic oxygen unloading conditions. We also use the model to simulate polymerization in almost completely deoxygenated sickle erythrocytes, validate the theoretical polymerization curves against published data, and compare them with the corresponding curves from the dynamic oxygen unloading analyses. Our model shows that equilibrium predictions severely overestimate intracellular polymer concentrations and underestimate cellular oxygen content, during the unloading of oxygen. Also, the delay times to significant polymerization in the physiologic situation are substantially longer than the corresponding values measured in completely deoxygenated HbS solutions. These results indicate that in vivo HbS polymerization is strongly influenced by the rate of oxygen desaturation. Equilibrium estimates of intracellular polymer content, or polymerization kinetic data from fully deoxygenated solutions, could be misleading and should be used in the proper perspective.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 1","pages":"169-83; discussion 184-90"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18988871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blood cells Pub Date : 1994-01-01

P M Lansdorp, W Dragowska, T E Thomas, M T Little, H Mayani

引用次数: 0

Retention and multilineage expression of human hematopoietic stem cells in human-sheep chimeras. 人羊嵌合体中人造血干细胞的保留和多系表达。

Blood cells Pub Date : 1994-01-01

E D Zanjani, M R Silva, A W Flake

引用次数: 0