Blood cells最新文献_第4页

Umbilical cord blood cell transduction by retroviral vectors: preclinical studies to optimize gene transfer. 逆转录病毒载体的脐带血细胞转导:优化基因转移的临床前研究。

Blood cells Pub Date : 1994-01-01

M E Hanley, J A Nolta, R Parkman, D B Kohn

{"title":"Umbilical cord blood cell transduction by retroviral vectors: preclinical studies to optimize gene transfer.","authors":"M E Hanley, J A Nolta, R Parkman, D B Kohn","doi":"","DOIUrl":"","url":null,"abstract":"Human umbilical cord blood (UCB) can be a source of hematopoietic stem cells for gene therapy, as an alternative to allogeneic bone marrow transplantation, for the treatment of a number of genetic diseases. To determine conditions that yield maximal gene transfer into UCB progenitor cells, we examined a number of variables. We used cell-free retroviral vector supernatants that convey neomycin (G418) resistance and measured the percentage of G418-resistant progenitor-derived colonies. Adding retroviral supernatant to the UCB cells in basal medium once a day for 3 days produced a threefold increase of G418-resistant colonies (9.8%) compared to a single exposure to supernatant (3.1%). To establish whether recombinant human growth factors are beneficial during transduction, the presence of interleukin-3 (IL-3), IL-6, and mast cell growth factor (MGF, a c-kit ligand) were compared in different combinations. Inclusion of the three factors together caused a threefold increase of gene transfer (30.4%) compared to transduction in basal medium. When the UCB cells were precultured in medium containing IL-3, IL-6, and MGF for 3 days before addition of the retroviral supernatant on days 4, 5, and 6, the average extent of gene transfer was 21.8%, compared with an average of 34.4% when UCB cells were transduced on days 1, 2, and 3. The presence of marrow stroma during the transduction of the UCB cells did not further increase gene transfer. We conclude that UCB progenitor cells can be efficiently transduced with the use of recombinant human growth factors IL-3, IL-6, and MGF and may be a suitable source for gene therapy.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"539-43; discussion 544-6"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18545694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary: hematopoietic progenitors in fetal and adult tissue. 评论:胎儿和成人组织中的造血祖细胞。

Blood cells Pub Date : 1994-01-01

L W Terstappen, F Lund-Johansen

引用次数: 0

Isolation and ex vivo expansion of CD34+ cells from cord blood using dextran sedimentation and avidin column selection. 右旋糖酐沉淀法和亲和素柱选择法在脐带血中分离和体外扩增CD34+细胞。

Blood cells Pub Date : 1994-01-01

S Heimfeld, D F Kalamasz, B L Fogarty, R Fei, Z N Tsui, H M Jones, R J Berenson

引用次数: 0

Proposed policies and procedures for the establishment of a cord blood bank. 建议建立脐带血银行的政策和程序。

Blood cells Pub Date : 1994-01-01

J McCullough, M E Clay, S Fautsch, H Noreen, M Segall, E Perry, D Stroncek

引用次数: 0

Commentary: cord blood banking for bone marrow reconstitution: a need for standardization and international cooperation. 评论:用于骨髓重建的脐带血库:需要标准化和国际合作。

Blood cells Pub Date : 1994-01-01

Van Rood

引用次数: 0

Engraftment of normal murine marrow into nonmyeloablated host mice. 将正常小鼠的骨髓移植到未清髓的宿主小鼠体内。

Blood cells Pub Date : 1994-01-01

P J Quesenberry, H Ramshaw, R B Crittenden, F M Stewart, S Rao, S Peters, P Becker, P Lowry, M Blomberg, J Reilly

引用次数: 0

Commentary: Immunohistochemistry represents a useful tool to study human cell engraftment in SCID mice transplantation models. 评论:免疫组织化学是研究人类细胞植入SCID小鼠移植模型的有用工具。

Blood cells Pub Date : 1994-01-01

A Orazi, S E Braun, H E Broxmeyer

引用次数: 0

Cord blood transplantation: implications for graft vs. host disease and graft vs. leukemia. 脐带血移植:移植物抗宿主病和移植物抗白血病的意义。

Blood cells Pub Date : 1994-01-01

D T Harris

{"title":"Cord blood transplantation: implications for graft vs. host disease and graft vs. leukemia.","authors":"D T Harris","doi":"","DOIUrl":"","url":null,"abstract":"Cord blood (CB) was analyzed for its alloreactive immune potential to evaluate its capacity to mediate graft vs. host disease (GVHD) and graft vs. leukemia (GVL) effects. Cord blood was observed to display minimal innate cytotoxic capacity but was capable of rapidly developing significant nonspecific natural killer cell-like effector mechanisms. Cord blood was unable to generate effective alloantigen-specific cytotoxic T lymphocytes (CTL), which was at least partially due to an altered lymphokine profile. The frequency of alloreactive T cells present in CB (whether assessed as total responding T-cell or CTL precursors) was greatly reduced as compared to adult peripheral blood lymphocytes (PBLs). However, the frequency of nonspecific effector cells was equivalent to PBLs. Significantly, CB T cells seemed to have undergone some type of developmental tolerance to maternal HLA antigens in utero, which could greatly increase the utility of CB in familial transplants. That is, CB T cells were unresponsive to noninherited maternal HLA antigens. Finally, CB demonstrated significant GVL capacity whether measured in vitro or in an animal model in vivo. Thus, the use of CB in most transplant settings should be free of the immunological problems associated with GVHD yet still be an effective mediator of GVL.","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"560-4; discussion 564-5"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18749585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allogeneic responses of human umbilical cord blood. 人类脐带血的异体反应。

Blood cells Pub Date : 1994-01-01

G Risdon, J Gaddy, H E Broxmeyer

引用次数: 0

Use of granulocyte-macrophage colony-stimulating factor in two children treated with cord blood transplantation. 粒细胞-巨噬细胞集落刺激因子在儿童脐带血移植中的应用。

Blood cells Pub Date : 1994-01-01

M R Vowels, K Tiedemann, R Lam-Po-Tang, D P Tucker

{"title":"Use of granulocyte-macrophage colony-stimulating factor in two children treated with cord blood transplantation.","authors":"M R Vowels, K Tiedemann, R Lam-Po-Tang, D P Tucker","doi":"","DOIUrl":"","url":null,"abstract":"Cord blood contains stem cells in amounts similar to or slightly less than those present in a bone marrow collection to be used for bone marrow transplantation (BMT). Too few cord blood transplants (CBT) have yet been performed to define the ability to achieve engraftment and the rate of engraftment. Two cord blood transplants have been performed using granulocyte-macrophage colony stimulating factor (GM-CSF) to hasten engraftment. Two children, aged 5 and 6 years received a CBT using HLA-identical stem cells collected at the birth of a sibling. One child had X-linked lymphoproliferative disease (XLP), and the other, acute lymphoblastic leukemia in second complete remission. One had an ABO and one an Rh blood group mismatch. Conditioning therapy consisted of cyclophosphamide, melphalan, and antithymocyte globulin or busulphan and cyclophosphamide. Graft-versus-host disease prophylaxis was methotrexate and cyclosporine or cyclosporine. Both children were given GM-CSF at 5 micrograms/kg/day from day 1 until the absolute neutrophil count (ANC) reached 1.0 x 10(9)/L for 3 consecutive days. If this level was not reached by day 14, the dose of GM-CSF was doubled. Both children engrafted rapidly, with ANCs reaching 0.5 x 10(9)/L in 12 and 16 days. Engraftment was confirmed by blood group in both and sex chromosome typing in one. Both children developed mild GVHD localized to skin, which resolved with steroid therapy. The child with XLP was cured and has survived for 34 months; the second child has survived 27 months with normal marrow function but has had a relapse of leukemia.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":75604,"journal":{"name":"Blood cells","volume":"20 2-3","pages":"249-54; discussion 254-5"},"PeriodicalIF":0.0,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18750393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0