Archivos de investigacion medica最新文献_第2页

Response of serum zinc and copper to inflammatory stimulation in the rat with chronic liver damage. 慢性肝损伤大鼠血清锌、铜对炎症刺激的反应。

Archivos de investigacion medica Pub Date : 1991-07-01

J Garduño Espinosa, R Paniagua Sierra, S Díaz Bensussen, A Lifshitz

{"title":"Response of serum zinc and copper to inflammatory stimulation in the rat with chronic liver damage.","authors":"J Garduño Espinosa, R Paniagua Sierra, S Díaz Bensussen, A Lifshitz","doi":"","DOIUrl":"","url":null,"abstract":"Objective of the study: To measure the seric levels of zinc and copper in healthy and in hepatic damaged rats when an inflammatory stimulus is applied.Research design: 2 x 2 factorial design.Subjects: Fisher *44 strain rats, healthy and with chronic hepatic damage.Measurements: Serum levels of Zn and Cu were determined in the different experimental conditions.Results: Basal Zn levels were found diminished in rats with hepatic damage as compared with healthy ones (p < 0.05), whereas Cu levels were found increased in hepatic damaged rats as compared with healthy ones (p < 0.05). Application of an inflammatory stimulus decreased Zn and increased Cu levels in healthy rats as compared to their basal conditions (p < 0.05), whereas no statistically significant variations were found in the group of rats with hepatic damage when comparing their serum levels before and after inflammatory stimulation.Conclusions: Rats with liver damage present alterations in their Zn and Cu levels under basal conditions. Rats with liver damage, submitted to an inflammatory stimulus, do not modify their serum Zn and Cu levels.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 3-4","pages":"289-94"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13005385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of chronic toluene inhalation on avoidance conditioned behavior in rats. 慢性甲苯吸入对大鼠回避条件行为的影响。

Archivos de investigacion medica Pub Date : 1991-07-01

L Castilla-Serna, R Rodríguez Pérez, F Osorio-Cruces, M E Sánchez-Hernández, A Gálvez Gutiérrez, J Cravioto

{"title":"Effects of chronic toluene inhalation on avoidance conditioned behavior in rats.","authors":"L Castilla-Serna, R Rodríguez Pérez, F Osorio-Cruces, M E Sánchez-Hernández, A Gálvez Gutiérrez, J Cravioto","doi":"","DOIUrl":"","url":null,"abstract":"Thirty-two Wistar albino rats were distributed at random in a factorial design 2 x 2, with two age groups (20 and 90 days old), and two treatments (chronically exposed to toluene vapors 30,000-40,000 ppm in air/15 minutes per day for 30 days, and controls with only air). Twenty-four hours after the last exposure, the rats were tested for conditioned behavior in a \"T\" maze of avoidance electric shock. Latency of initial response to escape (LIRE) and latency of escape (LE) were measured in seconds. Adult rats both exposed to chronic toluene inhalation and non-exposed showed higher values of LIRE and LE with respect young rats. Animals exposed to chronic toluene inhalation also presented higher values of latency in both LIRE and LE when compared to non-exposed to toluene (controls) of the same age. The differences were evaluated by Friedman's test. The findings are suggestive of brain dysfunction associated with chronic toluene inhalation, and may represent a delay to respond to a displeasure stimulus probably due to defect of internal mechanisms of facilitation-inhibition of neural impulses, mediated by neurotransmitters.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 3-4","pages":"295-301"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12853095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of age upon the increase in HDL2-cholesterol in the alcoholic. 年龄对酗酒者hdl2 -胆固醇升高的重要性。

Archivos de investigacion medica Pub Date : 1991-07-01

M Zentella de Piña, A Díaz Belmont, L Rodríguez-Lizarraga, E Piña

{"title":"Importance of age upon the increase in HDL2-cholesterol in the alcoholic.","authors":"M Zentella de Piña, A Díaz Belmont, L Rodríguez-Lizarraga, E Piña","doi":"","DOIUrl":"","url":null,"abstract":"An inverse relationship has been found between high density lipoprotein cholesterol (HDL-C) and the incidence of coronary disease. A controversy exists in the international literature as to which sub-fraction, HDL2-C or DHL-C, rises after ethanol ingestion. This paper studies a comparison of the levels of circulating cholesterol, HDL-C, HDL2-C and HDL3-C in two groups: a control group of 44 healthy subjects who had no ethanol in over a year, and a second one made up off 40 chronic alcoholics, who consumed between 80 and 160 gr. of ethanol per day. The alcoholic population showed lower levels of cholesterol and higher levels of HDL-C, HDL2-C and HDL3-C. When compared with the control group, the increase was in alcoholics 58% for HDL2-C and 29% for HDL3-C. An analysis of the different age groups shows an increase of 110% in HDL2-C, in alcoholics between ages 31 and 40, as compared with their control group. An increase of 81% occurred between ages 51 and 60, but rarely rose 20% between ages 21 and 30, as well as between 51 and 60. The maximum rise of HDL3-C in drinkers, related to their control group, was 38% during the fourth decade of life. The conclusion is that the HDL2-C subfraction rises in chronic alcoholics, and the changes in other HDL-C subfractions are more useful when they are placed at the different individual's decades of age, than when taken from complete population samples.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 3-4","pages":"323-7"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13005351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The (Ca2+, Mg2+)-ATPase activity in erythrocyte membranes from essential hypertensive patients. 原发性高血压患者红细胞膜(Ca2+， Mg2+)- atp酶活性的研究。

Archivos de investigacion medica Pub Date : 1991-07-01

A Gutiérrez, R Ariza, A C Frati-Munari, G Oliva, J Mas-Oliva

引用次数: 0

Captopril effect on non-diabetic glomerular albuminuria. 卡托普利对非糖尿病性肾小球蛋白尿的影响。

Archivos de investigacion medica Pub Date : 1991-07-01

L Nieto Cisneros, A Viniegra, G Castro-Martínez, J López-Barcena, N Wacher Rodarte

引用次数: 0

Partial characterization of Serratia marcescens nosocomial strains. 粘质沙雷菌医院菌株的部分特征。

Archivos de investigacion medica Pub Date : 1991-07-01

V R Coria-Jiménez, L Villa-Tanaka, C Ortíz-Torres

{"title":"Partial characterization of Serratia marcescens nosocomial strains.","authors":"V R Coria-Jiménez, L Villa-Tanaka, C Ortíz-Torres","doi":"","DOIUrl":"","url":null,"abstract":"A nosocomial infection outbreak occurred in the Intensive Care Unit (ICU) of the Instituto Nacional de Pediatría (INP) in México City, during the months of March, April and May in 1988 Serratia marcescens was isolated as the etiological agent for this epidemic. Up to date, the source of contamination, the spreading and the pathogenic mechanisms which were involved in this outbreak remain unknown. In order to study the dynamics of the bacterial population involved in this outbreak, all strains of nosocomial S. marcescens isolated during 1988 were collected and studied. Eighty nosocomial strains were analysed. For this purpose we used four different markers: antibiotic susceptibility, presence of plasmids, exoenzyme production and pigment synthesis from a precursor. Using these markers, we were able to establish that five subpopulations of bacteria were present during the ICU outbreak, and that one of these subpopulations, VIII-A, was the most frequently isolated. A short time after this outbreak, we obtained S. marcescens isolates with similar properties which proceeded from other hospital units, suggesting intrahospital dissemination of the strain in question. We believe that, eventually, this study will allow us to establish bacterial spreading models within our institution.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 3-4","pages":"273-8"},"PeriodicalIF":0.0,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13005386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toxigenicity and adherence in Clostridium difficile strains isolated from patients with and without diarrhoea. 从有腹泻和无腹泻患者分离的艰难梭菌菌株的毒力和粘附性。

Archivos de investigacion medica Pub Date : 1991-04-01

G González-Valencia, O Muñoz, J F Torres

{"title":"Toxigenicity and adherence in Clostridium difficile strains isolated from patients with and without diarrhoea.","authors":"G González-Valencia, O Muñoz, J F Torres","doi":"","DOIUrl":"","url":null,"abstract":"The mechanisms by which Clostridium difficile causes diarrhoea are unknown. The expression of putative virulence factors by 44 Clostridium difficile strains isolated from patients with and without diarrhoea was studied. Toxins A and B were tested in CHO and MRC-5 cells, respectively; adherence was measured in two substrates: HEp-2 cells and polystyrene plates. The in vitro expression of toxins A and B by strains isolated from patients with diarrhoea was not significantly different from that by strains isolated from patients without diarrhoea. The ability of adherence to both HEp-2 cells and polystyrene by strains isolated from patients with diarrhoea was not significantly different when compared with strains isolated from patients without diarrhoea; however, strains isolated from adults with diarrhoea seem to adhere to a greater extent to both substrates than strains isolated from adults without diarrhoea. Twenty three strains which did not produce toxins A and B were tested for enterotoxicity in rat small intestine. Eight such strains induced fluid accumulation and seven of them were isolated from children. Adherence to cells and to polystyrene might be an important virulence factor in strains causing diarrhoea in adults; whereas the production of toxins other than A and B might be an important pathogenic mechanism in strains causing diarrhoea in children.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"189-96"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12982182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Percutaneous transluminal ultrasonic angioplasty: preliminary clinical report of ultrasound plaque ablation in totally occluded peripheral arteries. 经皮腔内超声血管成形术:超声消融完全闭塞外周动脉斑块的初步临床报告。

Archivos de investigacion medica Pub Date : 1991-04-01

C Monteverde-Grether, M Valez y Tello de Meneses, G Nava-López, R Jauregui, J Borges, A Abundes, M López-Cuéllar, M Armilla

{"title":"Percutaneous transluminal ultrasonic angioplasty: preliminary clinical report of ultrasound plaque ablation in totally occluded peripheral arteries.","authors":"C Monteverde-Grether, M Valez y Tello de Meneses, G Nava-López, R Jauregui, J Borges, A Abundes, M López-Cuéllar, M Armilla","doi":"","DOIUrl":"","url":null,"abstract":"Peripheral arterial obstructive disease has been treated with ultrasound ablation. Thirty two patients (pts), mean age 67 +/- 10 years underwent angiosonoplasty, with a prototype ultrasonic system, due to severe claudication (31%), rest pain (44%), rest pain and ulceration (25%). Angiosonoplasty was successful in 26/32 (81%) stenoses, (1/2 iliac, 9/2 femorals, 11/12 popliteal, 5/6 tibial). Angiography before and after angiosonoplasty confirmed that the lesions were successfully opened, (81%, p less than 0.05). A mean of 3 +/- 2 passes were required to adequately remove sufficient plaque. Balloon angioplasty was used in combination with angiosonoplasty in 11/32 (34%) occluded vessels. Complications included groin hematoma in 4 pts (12%) and vascular spasm in two patients (6%). Within 24 hours, 27/32 (94%) pts were discharged clinically improved. Reocclusion occurred in 4 pts (12%) immediately after the procedure. Ankle brachial indices significantly increased from 0.52 +/- 0.10 before to 0.70 +/- 0.12 after (p less than 0.05). In conclusion, the use of ultrasound energy to ablate atherosclerotic plaque would appear safe, and has shown no restenosis and a low incidence of complications over a six month period.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"171-9"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13001579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3T3 cells in adipocytic conversion. 脂肪细胞转化中的3T3细胞。

Archivos de investigacion medica Pub Date : 1991-04-01

M S O'Shea Alvarez

{"title":"3T3 cells in adipocytic conversion.","authors":"M S O'Shea Alvarez","doi":"","DOIUrl":"","url":null,"abstract":"3T3 are murine cells of an established heteroploid cellular line. Some clones of this cellular line, when cultured under adequate conditions differentiate into adipocytes. During the process of differentiation, the cells undergo a change from the elongated fibroblastic shape to a round or oval form and accumulate small drops of lipids within their cytoplasma. These lipid drops fuse into one large drop which displaces the nucleus towards the periphery, giving the cell the aspect of a mature adipocyte of white adipose tissue. The cells not only change their morphology, but they also present important biochemical changes. They show a simultaneous increase in triglyceride synthesis and activity of lipogenic enzymes. There is also an increase in the response of the activity of various hormones and the de novo synthesis of the receptors to such hormones, as insulin and ACTH. During the process of differentiation important changes occur in the synthesis of various proteins, such as actin, tubulin, and other proteins which also make up the cellular cytoskeleton, forming part of the lipid transportation within the adipose cell. The adipocytic differentiation of 3T3 cells depends on adipogenic serum factors used in the supplementary culture medium. These adipogenic factors seem to play an important role in the development of adipose tissue. There are hormones, chemical agents and serum factors which modulate adipocytic differentiation. The clone must be susceptible to adipocytic differentiation, it must reach a quiescent state and find itself in adipogenic conditions for the 3T3 cells to differentiate into adipocytes. It must also carry out an DNA synthesis which is an expression of the new phenotype. The differentiation of 3T3 cells in terminal. The fact that these cells present an adipocytic conversion under physiologic conditions and with adipogenic hormones which exist in the whole animal has been demonstrated. All of these characteristics show that the 3T3 cells may be used as an adequate experimental system to analyze the events which occur during the differentiation and development of adipose tissue.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"235-44"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12889809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Mimosa tenuiflora bark extracts on WI38 and KB human cells in culture. 含羞草树皮提取物对WI38和KB人细胞的影响。

Archivos de investigacion medica Pub Date : 1991-04-01

M L Villarreal, P Nicasio, D Alonso-Cortés

引用次数: 0