Archivos de investigacion medica最新文献_第3页

Influence of progesterone and 17 alpha hydroxyprogesterone upon the BANA-hydrolytic activity of human sperm. 孕酮和17 α羟孕酮对人精子bana水解活性的影响。

Archivos de investigacion medica Pub Date : 1991-04-01

A Valencia-Sánchez, E Mercado-Pichardo

引用次数: 0

Detection of Plasmodium falciparum: a comparison of six cloned DNA probes. 恶性疟原虫检测:六种克隆DNA探针的比较。

Archivos de investigacion medica Pub Date : 1991-04-01

L E Andrade, L E Cardona, C Vergara

{"title":"Detection of Plasmodium falciparum: a comparison of six cloned DNA probes.","authors":"L E Andrade, L E Cardona, C Vergara","doi":"","DOIUrl":"","url":null,"abstract":"Probes pRepHind, Rep20, p242B1-1, pPF-14, clone 26 and 34 were compared for their applicability to detect P. falciparum in field conditions. Ninety four clinical samples from patients living in the malaria endemic area of Tumaco (Pacific Coast) plus 88 from Villavicencio (Eastern Plains) were tested in \"dotblot\" hybridization experiments. Probes Rep20, p242B1-1, pRepHind and pPF-14 detected up to 17 pg of purified P. falciparum DNA, while clone 26 and clone 34 detected up to 425 pg DNA. Probes pPF-14, P242B1-1, pRepHind and Rep 20 exhibited comparable detection levels of parasites in infected blood samples. Sensitivity declined from 69-94% in subjects with parasitemias higher than 10.000 par./ul to 15-42% in subjects with parasitemias lower than 100 par./ul. pPF-14 and p24B1-1 showed the highest sensitivity, while clone 26 and 34 presented significantly lower sensitivities. All probes were shown to be highly specific. Detection levels are dependent on specimen treatment. Treatment consisting of serum removal, Triton X-100 lysis, Proteinase K digestion, Phenol and Chloroform extractions followed by Ethanol precipitation yielded 100% sensitivity for specimens with parasite density higher than 1,000 par./l.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"181-7"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12982184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of nitroglycerin on rat myocardium oxygen consumption, determined by the manometric Warburg procedure. 硝酸甘油对大鼠心肌耗氧量的影响，用测压法测定。

Archivos de investigacion medica Pub Date : 1991-04-01

A Rangel Abundis, G Marín Osuna

引用次数: 0

The intraaortic counterpulsation balloon in cardiac surgery. The importance of opportune indication. 心脏手术中的主动脉内反搏球囊。适时适应症的重要性。

Archivos de investigacion medica Pub Date : 1991-04-01

O Sánchez-Ramírez, E Portilla-de Buen, R Verdin-Vázquez, R Argüero-Sánchez

引用次数: 0

Delayed insulin absorption due to subcutaneous edema. 皮下水肿导致胰岛素吸收延迟。

Archivos de investigacion medica Pub Date : 1991-04-01

C R Ariza-Andraca, E Altamirano-Bustamante, A C Frati-Munari, P Altamirano-Bustamante, A Graef-Sánchez

{"title":"Delayed insulin absorption due to subcutaneous edema.","authors":"C R Ariza-Andraca, E Altamirano-Bustamante, A C Frati-Munari, P Altamirano-Bustamante, A Graef-Sánchez","doi":"","DOIUrl":"","url":null,"abstract":"A prospective study to determine if subcutaneous edema interferes with insulin absorption was performed. Forty-six patients entered the study. Three groups were formed. Twenty patients with generalized edema (Group 1), ten of them with non-insulin dependent diabetes mellitus (NIDDM). Twenty patients without edema (Group II). 10 of them with NIDDM; and six patients with mild edema (Group III). The disappearance of I125-insulin was measured throughout 360 minutes. The rate of absorption in group I was significantly lower and delayed than in group II. The amount of insulin absorbed at 360 minutes was 3 to 4 fold lower in group I than in group II (p 0.001). Group III had intermediate values. The peak of plasma I125-insulin level was 3 to 4 fold lower in group I than group II. The impairment of insulin absorption in subjects with edema was more evident in those with NIDDM. In conclusion, this study demonstrates that subcutaneous edema impairs insulin absorption. Insulin absorption from subcutaneous tissue varies due to several conditions, resulting in a difficult glycemic control. Previous studies have shown that insulin absorption is affected by several factors as the site of injection, room and skin temperature, physical exercise, the thickness of adipose tissue, local massage, and local degradation of insulin. Edema due to chronic complications such as nephropathy and cardiopathy often occurs in long-standing diabetic subjects. However, the effects of edema of the skin and subcutaneous tissue on insulin absorption has not been previously examined. The aim of this study was to assess if edema affects the absorption of insulin.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"229-33"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12981333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of stress in male fertility. 压力在男性生育能力中的作用。

Archivos de investigacion medica Pub Date : 1991-04-01

R Alonso-Uriarte, I Sojo-Aranda, V Cortés-Gallegos

引用次数: 0

Role of leukotriene C4 and edema in the acute allergic bronchoconstriction in the guinea pig. 白三烯C4和水肿在豚鼠急性变应性支气管收缩中的作用。

Archivos de investigacion medica Pub Date : 1991-04-01

M H Vargas, M Selman, G Campos, L M Montaño

{"title":"Role of leukotriene C4 and edema in the acute allergic bronchoconstriction in the guinea pig.","authors":"M H Vargas, M Selman, G Campos, L M Montaño","doi":"","DOIUrl":"","url":null,"abstract":"In vitro studies have suggested that leukotrienes are involved in acute allergic bronchoconstriction, though this has not been definitively corroborated yet in in vivo studies. On the other hand, edema production during antigenic challenge could be an additional factor favouring such bronchoconstriction. In the present work we quantified immunoreactive leukotriene C4 (iLTC4) concentrations in bronchoalveolar lavages during allergic bronchoconstriction induced by 1 mg/kg i.v. ovalbumin (OA) in immunized guinea pigs, as well as water content in guinea pig lung fragments obtained before and during this bronchoconstriction. We found that basal concentrations of iLTC4 (median 1.06 ng/ml) were not significantly modified at 2, 5 and 10 min (median 1.10, 0.29 and 1.37 ng/ml, respectively) of the bronchoconstrictor response. Water content in lung fragments did not change among non-immunized guinea pigs, immunized ones and at 15 min of bronchoconstriction (mean +/- SEM 79.32% +/- 0.18, 79.10% +/- 0.31 and 79.13% +/- 0.40%, respectively). In addition, isoproterenol (20 micrograms/kg, i.v.) rapidly reverted about 70% of the bronchoconstriction induced by a higher antigenic dose (OA, 3.1 mg/kg i.v.); residual obstruction was not associated with increased water content in lung fragments (78.13% +/- 0.43). These results suggest that in this model, acute allergic bronchoconstriction is not due to an increased iLTC4 release or to edema production, and that airway smooth muscle contraction is the main component of this response.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"143-9"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12982179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunoreactive insulin levels in ankylosing spondylitis. 强直性脊柱炎的免疫反应性胰岛素水平。

Archivos de investigacion medica Pub Date : 1991-04-01

F J Jiménez-Balderas, J L Solís, G Mintz

引用次数: 0

Percutaneous transluminal ultrasonic angioplasty: preliminary clinical report of ultrasound plaque ablation in totally occluded peripheral arteries. 经皮腔内超声血管成形术:超声消融完全闭塞外周动脉斑块的初步临床报告。

Archivos de investigacion medica Pub Date : 1991-04-01 DOI: 10.1007/978-1-4613-1243-7_10

C. Monteverde-Grether, M. Valez y Tello de Meneses, G. Nava-López, R. Jáuregui, J. Borges, A. Abundes, M. López-Cuéllar, M. Armilla

引用次数: 1

Morphogenesis of the gubernaculum testis in Sprague-Dawley rat. Preliminary report. Sprague-Dawley大鼠睾丸管骨的形态发生。初步报告。

Archivos de investigacion medica Pub Date : 1991-04-01

G Arredondo, M A Arreola-Salinas, W Lierse

{"title":"Morphogenesis of the gubernaculum testis in Sprague-Dawley rat. Preliminary report.","authors":"G Arredondo, M A Arreola-Salinas, W Lierse","doi":"","DOIUrl":"","url":null,"abstract":"The morphology of testicular descent in the Sprague-Dawley rat from day 14 of gestation to newborn was studied; the purpose was to analyse the anatomy and related observations made with light and scanning microscopy. Adult female rats were mated during estrus. The presence of spermatozoa in vaginal smears was taken to indicate day 0 of gestation. Animals were kept in a controlled 12-hour light-darkness cycle; food and water were provided ad libitum. At day 14 genital ducts are similar in both sexes. At day 15 and 16 the gubernaculum testis is seen as a mesenchymal cord that extends from mesonephros to the pelvic floor. At day 17 near the enlarged caudal end of gubernaculum, evagination of the abdominal cavity begins forming the peritoneo-vaginal process. The testis ligament as well as the gubernaculum testis attaching to the epididymis at different locations have no direct relation between themselves as such. During day 18, testis rotates on its own longitudinal axis in a ventral-lateral direction. This movement twists the testis ligament, the epididymis and deferent duct lengthen and these structures are lined up in the direction of the peritoneo-vaginal process. The testis is generally situated in the newborn, on or near the internal ring. The gubernaculum testis is attached to the epididymis but does not exercise traction on the testis. The epididymis is a factor in this descending process.","PeriodicalId":75554,"journal":{"name":"Archivos de investigacion medica","volume":"22 2","pages":"151-61"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12982180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0