AIDS research and human retroviruses最新文献

{"title":"Experiences and Acceptability of Serial Lymph Node and Gut Biopsies Among Young Women Enrolled in an HIV Cure-Related Trial in Durban, South Africa.","authors":"Mzwakhe Wiseman Ngcobo, Ntombifuthi Langa, Ayanda Zulu, Whitney Tran, Vanessa Pillay, Maud Mthembu, Lihle Ngcongo, Ali Ahmed, Deborah Mindry, Nithin Lutchminarain, Zaza Ndhlovu, Megha L Mehrotra, Devi Sengupta, Elena Vendrame, Thumbi Ndung'u, Karine Dubé, Krista L Dong","doi":"10.1177/08892229261491477","DOIUrl":"10.1177/08892229261491477","url":null,"abstract":"<p><p>Human immunodeficiency virus (HIV) persists in diverse body tissues, including the gut, lymph nodes (LNs), and lungs, requiring direct sampling to characterize heterogeneous reservoirs. HIV cure-related trials may include tissue sampling to evaluate intervention efficacy and mechanisms to eliminate viral reservoirs. Repeated invasive sampling carries risks that must be balanced against safety, acceptability, and scientific value. We assessed the experiences of young women undergoing serial excisional LN and gut biopsies during an HIV cure-related trial in South Africa. We conducted a qualitative secondary analysis of in-depth interviews (IDIs) with women enrolled in a Phase 2A HIV cure-related trial (NCT05281510) that included an analytical treatment interruption (ATI). We conducted IDIs at enrollment and pre-ATI at the Females Rising through Education, Support and Health research site in Durban, South Africa, in <i>isiZulu</i>. We audio-recorded, transcribed, translated, and deidentified the interviews and analyzed them using inductive conventional content analysis. We interviewed 19 participants. Each underwent LN excision 1-3 times (mean 2.6) and gut biopsy 0-3 times (mean 2.5). Motivations included scientific curiosity and contributing to future generations. LN excision was generally painless, although some reported itching and slow healing. Gut biopsies caused abdominal pressure and transient bloating. One adverse event occurred among 48 LN excisions. Initial apprehension was common, but clear communication, supportive staff, and recognition of contributions fostered trust and willingness to undergo repeat procedures. Serial tissue sampling was acceptable among young South African women despite some discomfort. Altruism was a primary motivator, while clear communication and participant-centered care were critical to supporting informed participation and repeat sampling.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"8892229261491477"},"PeriodicalIF":1.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13659307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Two Novel HIV-1 Unique Recombinant Forms Involving CRF07_BC and CRF08_BC Among ART-Failing Patients in Hebei, China.","authors":"Haoxi Shi, Juan Meng, Yimin Cao, Yiteng Zhao, Ying Xing, Jian Du, Shangyu Li, Ziting Liu, Yuping Chen, Feng Zhao","doi":"10.1177/08892229261490627","DOIUrl":"https://doi.org/10.1177/08892229261490627","url":null,"abstract":"<p><p>The continuous HIV-1 recombination forms circulating in China poses a persistent challenge to epidemic control. And this study identifies two novel unique recombinant forms (URFs), BDNY017 and BDK025, from antiretroviral therapy (ART)-failing patients in Hebei Province. Near full-length genomic analysis revealed that BDNY017 is a triple mosaic of CRF08_BC, CRF07_BC, and subtype B, whereas BDK025 is a dual recombinant of CRF07_BC and CRF08_BC. Genotypic resistance testing further uncovered M184V, A98G, and E138K mutations in BDNY017, while BDK025 carried no major <i>pol-region</i> resistance mutations. Notably, the report of CRF08_BC-involved URFs in northern China, expanding the known geographic distribution of such recombinants beyond their traditional southwestern focus and underscoring the urgent need for enhanced molecular surveillance, particularly among ART-failing individuals, to track emerging recombinants and assess their potential impact on treatment outcomes and transmission dynamics.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"8892229261490627"},"PeriodicalIF":1.0,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of rs7975232 (ApaI) and rs731236 (TaqI) <i>VDR</i> Polymorphisms on HIV/AIDS Prevalence and Mortality: An International Population Genetics Analysis.","authors":"Nermin Akcali, Cüneyd Yavas, Sajjad Eslamkhah, Sonat Sina Canturk, Kevser Eryildiz, Nisa Aslan, Zeynep Nur Ozcan, Lutfiye Karcioglu Batur","doi":"10.1177/08892229261487810","DOIUrl":"https://doi.org/10.1177/08892229261487810","url":null,"abstract":"<p><p>In this study, the relationship between the allele and genotype frequencies of the rs7975232 (ApaI) and rs731236 (TaqI) polymorphisms of the vitamin D receptor (<i>VDR</i>) gene and country-based HIV/AIDS prevalence and mortality rates were examined using Spearman rank correlation analyses. These Spearman analyses were validated for significance using the False Discovery Rate (FDR) method, considering traditional criteria (Adjusted <i>p</i>-value <.05). The findings revealed that ApaI_CC and TaqI_TT genotype frequencies showed weak inverse correlations with HIV/AIDS prevalence and mortality rates (ρ ≈ -0.30 and -0.25); however, these correlations were not statistically significant after FDR correction (<i>p</i>_adj > 0.05). These results suggest that <i>VDR</i> polymorphisms showed no statistically significant association with HIV/AIDS epidemiology in this ecological analysis; at most, these findings generate a population-level hypothesis that vitamin D-mediated immune responses may warrant further study. These findings should be regarded as exploratory and hypothesis-generating, and may inform future individual-level genotype-phenotype and functional studies.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"8892229261487810"},"PeriodicalIF":1.0,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Factors Associated with Short-Term Decline in Estimated Glomerular Filtration Rate in People Living with HIV Receiving Antiretroviral Therapy: A Prospective Cohort Study.","authors":"Xenia Choc-Cano, Maricarmen Pelayo-Madrigal, Eduardo Gutiérrez-Maya, Ivan Zepeda-Quiroz, Sebastián Toledo-Ramírez, Barrera-Mota Brenda, Jimenez-Baez Valeria, Ciria Vázquez-Macias, Daniel Juárez-Villa","doi":"10.1177/08892229261487815","DOIUrl":"https://doi.org/10.1177/08892229261487815","url":null,"abstract":"<p><p>HIV infection remains a significant public health challenge. Although antiretroviral therapy has substantially improved survival, chronic complications such as renal impairment increase morbidity and mortality, making the estimated glomerular filtration rate (eGFR) crucial for early detection. This prospective, observational, and analytical cohort study aimed to identify the clinical, sociodemographic, and laboratory factors associated with short-term eGFR decline in people living with HIV (PLWH) over a 6-month follow-up at a second-level Mexican hospital. We included 166 PLWH aged > 18 years, collecting data on serum creatinine, eGFR, viral load, and CD4⁺ T cell counts. Patients were stratified based on the magnitude of eGFR decline into four groups (<5, 5-9, 10-19, and ≥20 mL/min/1.73 m²). The median age was 35 years (91% male). Median eGFR was 101.81 mL/min/1.73 m² at baseline and 100.54 mL/min/1.73 m² at 6 months. Most patients achieved virological suppression, and only 2.4% developed chronic kidney disease. Group analysis revealed a significant association between a greater short-term eGFR decline and advanced age, unemployment, and opportunistic infections. In conclusion, short-term renal decline was primarily associated with older age and unemployment. Although chronic kidney disease incidence was low, evaluating short-term eGFR decline allowed for the identification of clinically relevant alterations, highlighting the importance of periodic renal monitoring, particularly in clinically and socially vulnerable patients.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"8892229261487815"},"PeriodicalIF":1.0,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perceived Ethical Risks of HIV Phylogenetic Research: Insights from Professional Stakeholders in Uganda.","authors":"Farirai Mutenherwa, Tom Lutalo, Ronald M Galiwango, Jennifer Brophy Feltes, Jade Jackson, Oliver Laeyendecker, Nelson K Sewankambo, Jeremy Sugarman, Mary Kate Grabowski","doi":"10.1177/08892229261455943","DOIUrl":"10.1177/08892229261455943","url":null,"abstract":"<p><p>HIV phylogenetics is a powerful tool for elucidating HIV transmission dynamics, but ethical concerns have been raised about its use. We conducted a cross-sectional online survey among HIV professional stakeholders in Uganda to explore their knowledge of HIV phylogenetics and associated ethical considerations. Thirty-two participants completed the survey. 87% correctly defined HIV phylogenetics, but some were unable to recognize potential individual harms associated with HIV phylogenetic research (HPR). The most common individual and societal harms of HPR recognized were identification as an HIV transmission source 25 (78%; 95% CI, 61-89%) and marginalization of certain groups 27 (84%; 95% CI, 68-93%), respectively. Most strongly agreed that obtaining community assent 17 (53%; 95% CI, 36-69%) and specific informed consent for viral sequences used in HPR 20 (63%; 95% CI, 45-77%) were critical. Risks associated with HPR appear to be incompletely understood, underscoring the need to raise awareness of them among various professional stakeholders.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"260-263"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13643410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148142435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a Complex HIV-1 Circulating Recombinant Form (CRF203_cpx) Originating from Three Main Circulating Recombinant Forms in China.","authors":"Min Chen, Huichao Chen, Yanling Ma, Wenfei Ding, Manhong Jia","doi":"10.1177/08892229261463834","DOIUrl":"10.1177/08892229261463834","url":null,"abstract":"<p><p>Yunnan Province is recognized as a hotspot for the emergence of HIV-1 recombinant forms due to its complex epidemiological history. This study identified a novel circulating recombinant form (CRF) of HIV-1 in the region, designated as CRF203_cpx. Near-full-length genome sequences were obtained from three individuals infected through heterosexual contact in Wenshan Prefecture, an area located near the border with Vietnam. These sequences formed a distinct monophyletic clade with strong bootstrap support, separate from all known subtypes and CRFs. The genome of this strain can be divided into 14 subregions: S1, S4, S7, and S8 were derived from CRF55_01B; S2, S11, and S13 from CRF07_BC; and S3, S5, S6, and S12 from CRF01_AE. S9, S10 and S14 were likely derived from recombination between CRF01_AE and CRF55_01B. Bayesian Markov chain Monte Carlo analysis indicated that CRF203_cpx emerged between 2018 and 2021. To the best of our knowledge, this is the first time that a complex CRF comprising three CRFs (CRF01_AE, CRF07_BC, and CRF55_01B) has been definitively identified in China. This reveals the dynamic process by which circulating strains accumulate evolutionary changes through multilevel recombination, raising new considerations for current surveillance and control strategies.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"270-274"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluctuating Immune Reconstitution and Delayed HIV-1 DNA Decline in People Living with HIV with Low CD4 on Bictegravir-Based Therapy.","authors":"Jin Li, Minghua Zhou, Huizhuan Guo, Lishan Huang, Qingsong Qi, Qingyang Zhong, Huijun Zhong, Yanhao Wang, Meina Tian, Wenjie Xu, Wenrui Li, Wanxia Li","doi":"10.1177/08892229261468110","DOIUrl":"10.1177/08892229261468110","url":null,"abstract":"<p><p>This prospective cohort study compared the efficacy of bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) and tenofovir/lamivudine/efavirenz (TLE) regimens in 88 treatment-naïve persons living with HIV. The BIC regimen achieved faster HIV-1 RNA suppression at week 12, with comparable suppression thereafter. Regarding HIV-1 DNA, the TLE group exhibited a more rapid decline, largely attributable to its higher baseline levels, while both groups converged to comparable levels at week 48; this difference was no longer significant after adjusting for baseline HIV-1 DNA levels. Notably, the BIC group had significantly lower baseline CD4⁺ counts, reflecting the real-world inclusion of individuals with more advanced disease. Cluster analysis within the BIC group revealed three distinct DNA decay trajectories. A subset of individuals with the lowest baseline CD4⁺ counts (Cluster 1, <i>n</i> = 16) showed stable HIV-1 DNA through week 48 followed by significant decline only after 72 weeks, with CD4/CD8 ratio recovery preceding the decline. The early DNA plateau may reflect the interplay between immune reconstitution and viral reservoir dynamics. These findings suggest that baseline immune status influences early viral reservoir dynamics and that the timing of reservoir reduction may be linked to immune restoration, highlighting the heterogeneity of integrase inhibitor responses and the potential value of baseline immune assessment in guiding individualized management.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"253-259"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Conserved Natural Killer Cell Transcriptional Fingerprint in Rhesus Macaque Models of Acute Viral Infections.","authors":"Andrew Hudson, Kyle Kroll, Benjamin Bimber, Brady Hueber, Griffin Woolley, R Keith Reeves","doi":"10.1177/08892229261460539","DOIUrl":"10.1177/08892229261460539","url":null,"abstract":"<p><p>Natural killer (NK) cell activation and function are controlled by highly complex transcriptional programs. To leverage NK cells against human immunodeficiency virus and other diseases, a more complete understanding of signaling and metabolic dynamics is needed. In this study, we utilize a rhesus macaque (RM) model to define the transcriptomic profile of NK cells during acute lentiviral infection and to map the changes in this profile as infection progresses to the early chronic phase. Using a novel summary gene set for NK cell cytokine stimulation, we report synchronous metabolic and signaling transcriptional patterns that underlie established features of NK cell dysfunction. Assessing gene expression induced by the SARS-CoV-2 challenge allowed us to confirm a set of core conserved gene pathways central to RM NK cell activation across diverse acute viral contexts.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"249-252"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13283060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148215951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near Full-Length Genome Characterization of a Novel Second-Generation HIV-1 CRF01_AE/CRF07_BC Recombinant Identified in an MSM Individual in Guizhou, China.","authors":"Zhangwen Ge, Dan Liu, Hengzhi Zhong, Zhijie Niu, Haiyan Zhang, Qifeng Li, Jinding Zheng","doi":"10.1177/08892229261463837","DOIUrl":"10.1177/08892229261463837","url":null,"abstract":"<p><p>The cocirculation of CRF01_AE and CRF07_BC among men who have sex with men (MSM) in China may facilitate the emergence of genetically complex HIV-1 recombinants. Here, we identified and characterized a novel second-generation HIV-1 CRF01_AE/CRF07_BC recombinant, designated GY0192, from a 25-year-old MSM individual in Guiyang, Guizhou Province, southwest China, using near full-length genome (NFLG) analysis. Recombination analyses identified four breakpoints at HXB2 positions 3,125, 5,687, 6,375, and 9,176, generating a distinct five-segment mosaic genome. Subregion phylogenetic analyses showed that the two CRF01_AE-derived fragments clustered with the CRF01_AE cluster 5 lineage, while all three CRF07_BC-derived fragments grouped with CRF07_BC lineages frequently circulating among MSM in China, indicating that both parental components of GY0192 were phylogenetically related to lineages frequently reported among MSM populations in China. The virus was also predicted to be CCR5-tropic, adding epidemiological relevance because CCR5-tropic viruses are commonly involved in transmission and early infection. Together, these findings identify GY0192 as a distinct CRF01_AE/CRF07_BC mosaic and suggest that cocirculating MSM-associated HIV-1 lineages may provide opportunities for interlineage recombination. This case expands the known spectrum of CRF01_AE/CRF07_BC recombinants in Guizhou and underscores the value of NFLG-based surveillance for detecting underrecognized HIV-1 genetic complexity in southwest China.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"264-269"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolving Indications and Diagnostic Spectrum of Bone Marrow Examination in People with HIV During the Antiretroviral Therapy Era: A 6-Year Retrospective Study.","authors":"Jinglan Lai, Yahong Chen","doi":"10.1177/08892229261460535","DOIUrl":"10.1177/08892229261460535","url":null,"abstract":"<p><p>With the widespread use of antiretroviral therapy, the clinical spectrum among people with HIV has evolved significantly. This study aims to investigate the dynamic changes in the primary indications and final diagnostic spectrum of bone marrow examinations in this population. We conducted a retrospective analysis of 154 hospitalized individuals with HIV who underwent bone marrow examination at Mengchao Hepatobiliary Hospital of Fujian Medical University, the largest designated HIV/AIDS care hospital in Southeast China, from September 2019 to December 2025. Demographic, laboratory, and pathological data were collected, and participants were stratified into tumor and nontumor groups based on final diagnosis for comparative analysis. Among the indications for bone marrow examination, the proportion of procedures performed due to cytopenia decreased from 55.55% in 2019 to 17.86% in 2025. Conversely, the proportion performed for suspected hematological malignancy or to assess tumor marrow infiltration increased significantly from 11.11% to 60.71%. Final diagnoses revealed that the proportion of malignant tumors increased from 11.11% to 53.57%, whereas the proportion of opportunistic infections showed a declining trend. The Cochran-Armitage trend test confirmed that the proportion of opportunistic infections decreased significantly (<i>p</i> = .002), while the proportion of malignancies increased significantly (<i>p</i> = .002). Compared to the nontumor group, individuals in the tumor group were older, had significantly higher proportions receiving antiretroviral therapy, higher rates of HIV RNA suppression, as well as significantly higher CD4⁺ T-cell counts, CD8⁺ T-cell counts, lymphocyte counts, platelet counts, and lactate dehydrogenase levels. Our findings demonstrate that the clinical indications for bone marrow examination in people with HIV have shifted from the evaluation of traditional opportunistic infections and unexplained cytopenia toward the diagnosis and staging of malignant tumors, particularly hematological malignancies. These results suggest that in the contemporary management of HIV, greater emphasis should be placed on screening for malignancies.</p>","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"243-248"},"PeriodicalIF":1.0,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148222548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}