AIDS research and human retroviruses最新文献

Indicators for Increased Likelihood of Epidemic Kaposi Sarcoma Progression after Antiretroviral Therapy Initiation.

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-03 DOI: 10.1089/aid.2025.0007

David J Nolan, Gary B Fogel, Jonathan DaRoza, Rebecca Rose, Paige M Bracci, Susanna L Lamers, Michael S McGrath

{"title":"Indicators for Increased Likelihood of Epidemic Kaposi Sarcoma Progression after Antiretroviral Therapy Initiation.","authors":"David J Nolan, Gary B Fogel, Jonathan DaRoza, Rebecca Rose, Paige M Bracci, Susanna L Lamers, Michael S McGrath","doi":"10.1089/aid.2025.0007","DOIUrl":"https://doi.org/10.1089/aid.2025.0007","url":null,"abstract":"Kaposi sarcoma (KS) is a common malignancy for people living with HIV (PLWH), despite antiretroviral therapy (ART). Curiously, even with improved CD4+ T-cell counts and low viral loads following ART, some PLWH with KS may still experience KS progression or even death and require adjuvant chemotherapy to manage their KS. The factors associated with persistent or unresponsive KS after ART initiation remain poorly characterized, and biomarkers to identify patients at risk of KS progression are needed, particularly in resource-limited areas where access to chemotherapy is limited. Here we analyzed baseline KS tumor biopsies from PLWH with KS who required chemotherapy due to unresolved KS after ART initiation and those who did not require chemotherapy after ART initiation. By examining participant metadata and viral copy number for Kaposi sarcoma-associated herpesvirus (KSHV), HIV, cytomegalovirus, and Epstein-Barr virus and KSHV gene expression in the tumor biopsies prior to ART initiation, we identified a model of factors associated with KS progression after ART initiation, including biological sex, age, and the log ratio of KSHV/HIV copy number in the tumor. We believe that the ratio of KSHV/HIV may be linked to the cell types that each virus infects, and future work exploring the relationship between tumor and immune cells in the baseline tumors is planned. Innovation would be necessary to reduce costs and simplify the viral quantification assays, enabling the translation of these findings into routine clinical care, particularly in resource-limited settings.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of HIV-1 RNA and HIV-1 DNA Genotypic Drug Resistance Testing in Women of Childbearing Age Infected with HIV-1 in Liangshan Prefecture. 凉山州育龄妇女HIV-1感染HIV-1 RNA与HIV-1 DNA基因型耐药检测比较

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2024-12-24 DOI: 10.1089/aid.2024.0001

Bianchuan Cao, Mei Liu, Shaofang Song, Ping Ding, Fuli Huang, Yongmao Huang, Yongsheng Zou, Li Zhong

{"title":"Comparison of HIV-1 RNA and HIV-1 DNA Genotypic Drug Resistance Testing in Women of Childbearing Age Infected with HIV-1 in Liangshan Prefecture.","authors":"Bianchuan Cao, Mei Liu, Shaofang Song, Ping Ding, Fuli Huang, Yongmao Huang, Yongsheng Zou, Li Zhong","doi":"10.1089/aid.2024.0001","DOIUrl":"10.1089/aid.2024.0001","url":null,"abstract":"This study focuses on women of childbearing age infected with HIV-1 in Liangshan Prefecture and analyses their HIV-1 RNA and HIV-1 DNA genotypic drug resistance to provide a theoretical basis and technical support for monitoring the spread of resistant strains and formulating and optimizing antiretroviral therapy regimens. The study subjects were women of childbearing age infected with HIV-1 who were followed up in the county of Liangshan Prefecture from January to September 2023. Peripheral venous blood samples were collected from each subject. The samples were centrifuged to separate the plasma and blood cells for HIV-1 RNA quantitative testing and HIV-1 genotypic drug resistance testing. A total of 47 participants were included in this study. When HIV-1 RNA were <50 copies/mL and between 50 and 1,000 copies/mL, the success rate of HIV-1 DNA pol gene amplification was significantly higher than that of HIV-1 RNA pol gene amplification. Among the 47 subjects, 17 (17/47, 36.17%) indicated successfully amplified HIV-1 RNA and HIV-1 DNA genotypic drug resistance in each region simultaneously, and 9 (9/17, 52.94%) developed any degree of resistance. Among these nine cases, five had consistent resistance, while four indicated inconsistent resistance. Among the five cases with identical drug resistance, there were three cases with inconsistent drug resistance mutations (DRMs). Among the four cases with inconsistent drug resistance results, one had DRMs at the HIV-1 DNA level but no DRMs at the HIV-1 RNA level, while the other three had more DRMs at the HIV-1 RNA level than at the HIV-1 DNA level. The combination of HIV-1 RNA and HIV-1 DNA genotypic drug resistance testing can improve the drawbacks of current single HIV-1 RNA genotypic drug resistance testing, especially when HIV-1 RNA is ≤1,000 copies/mL, and significantly improve the efficiency of HIV-1 genotypic drug resistance testing.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"203-210"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV-1 DNA Genotypic Drug Resistance Testing Guides Antiretroviral Therapy in Patients with Low-Level Viremia. HIV-1 DNA基因型耐药检测指导低水平病毒血症患者的抗逆转录病毒治疗。

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-15 DOI: 10.1089/aid.2024.0088

Bianchuan Cao, Mei Liu, Shaofang Song, Mingxian Guo, Lingyu Tang, Ping Ding, Tianru Yuan, Tong Wang, Li Zhong

{"title":"HIV-1 DNA Genotypic Drug Resistance Testing Guides Antiretroviral Therapy in Patients with Low-Level Viremia.","authors":"Bianchuan Cao, Mei Liu, Shaofang Song, Mingxian Guo, Lingyu Tang, Ping Ding, Tianru Yuan, Tong Wang, Li Zhong","doi":"10.1089/aid.2024.0088","DOIUrl":"10.1089/aid.2024.0088","url":null,"abstract":"In 2023, we published a case study involving a 10-year-old child infected with HIV-1 with low-level viremia (LLV). We showed that this child patient achieved successful viral suppression by modifying the antiretroviral therapy (ART) regimen according to the HIV-1 DNA genotypic drug resistance testing. In this study, we aimed to address whether HIV-1 DNA genotypic drug resistance testing could direct successfully virological suppression in patients infected with HIV-1 experiencing persistent LLV based on evidence from a cohort study. The subjects of this study were all people living with HIV-1 who received ART and followed in the Yuexi County (Liangshan, China) from December 2010 to February 2024. From June 2021 to February 2024, a total of 10 mL of peripheral blood was collected from each subject at each follow-up and separated. HIV-1 RNA and HIV-1 DNA were quantified, followed by HIV-1 genotypic drug resistance testing. ART regimens were accordingly adjusted, while follow-up tests were performed in terms of HIV-1 RNA and DNA measurements. The prevalent HIV-1 DNA drug resistance mutations (DRMs) included M184V, K103N, K101E/P, and V108I. The primary resistance mutations observed for nucleoside reverse transcriptase inhibitor (NRTI) were against abacavir, lamivudine, and emtricitabine. For non-NRTI, the primary DRMs were associated with efavirenz and nevirapine. Five out of the six patients were subjected to regimen adjustments according to HIV-1 DNA DRMs, while one patient was continuously treated with unchanged regimen. Viral suppression was achieved in all five ART-changed cases, with observation of remarkable of HIV-1 DNA decline. The ART-unchanged case showed progressive treatment failure with drastic increase of plasma HIV-1 RNA and whole blood HIV-1 DNA. For patients with LLV, HIV-1 DNA genotypic drug resistance testing directed ART regimen considerations are highly recommended for achieving viral suppression.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"197-202"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Network Characteristics and Drug Resistance Analysis Among Newly Diagnosed Persons Living with HIV-1 in Hefei, China (2017-2022).

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-02-18 DOI: 10.1089/aid.2024.0093

Yu Shi, Tingting Zhang, Hui Yao, Hai Wang, Yanhua Lei, Qin Fang, Chenxi Shuai, Yizu Qin, Lifeng Miao, Lin Jin, Jin Zhang, Seying Dai, Yuelan Shen, Hui Xing, Yi Feng, Jianjun Wu

引用次数: 0

Molecular HIV Surveillance: Beyond Cluster Detection and Response.

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-24 DOI: 10.1089/aid.2024.0084

Sanjay R Mehta, Antoine Chaillon, Alan B Wells, Susan J Little

引用次数: 0

Sequence Notes: Identification of Three Novel HIV-1 Recombinant Strains with Different Recombination Patterns in Hebei Province, China.

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-24 DOI: 10.1089/aid.2024.0115

Sisi Chen, Haoxi Shi, Zhen Zhang, Lijuan Yin, Jianru Jia, Weiguang Fan

{"title":"Sequence Notes: Identification of Three Novel HIV-1 Recombinant Strains with Different Recombination Patterns in Hebei Province, China.","authors":"Sisi Chen, Haoxi Shi, Zhen Zhang, Lijuan Yin, Jianru Jia, Weiguang Fan","doi":"10.1089/aid.2024.0115","DOIUrl":"10.1089/aid.2024.0115","url":null,"abstract":"The global human immunodeficiency virus 1 (HIV-1) pandemic is driven by the extraordinary genetic diversity of the virus, largely resulting from frequent recombination events. These events generate circulating recombinant forms (CRFs) and unique recombinant forms, which significantly contribute to the complexity of HIV-1 epidemiology, especially within key populations, such as men who have sex with men (MSM). Here, we identified three novel HIV-1 recombinant strains consisting of the CRF01_AE and CRF07_BC subtypes from HIV-positive MSM in Baoding City, Hebei Province, China. Using near-full-length genome analysis and phylogenetic reconstruction, the strains-designated BDL017, BDL036, and BDSB006-were shown to exhibit distinct mosaic structures. Each strain contained multiple inserted fragments from CRF07_BC and CRF01_AE within various genomic regions, highlighting their complex recombination patterns. Our study emphasizes the need for continuous molecular surveillance among MSM in Hebei Province to monitor these recombinant forms and prevent their spread to the broader population.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"225-231"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of an Invisible HIV-1 Circulating Recombinant Form (CRF149_01B) in China. 中国HIV-1隐形循环重组形式（CRF149_01B）的鉴定

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-08 DOI: 10.1089/aid.2024.0099

Min Chen, Huichao Chen, Jie Dai, Lijuan Dong, Yanling Ma, Manhong Jia

引用次数: 0

Confirmation of Two Novel HIV-1 CRF01_AE/CRF07_BC Recombinant Forms Among Men Who Have Sex with Men in Hebei, China. 河北省男男性行为人群中两种新型HIV-1 CRF01_AE/CRF07_BC重组形式的确认

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-08 DOI: 10.1089/aid.2024.0104

Yapeng Guan, Jun Wang, Meng Liu, Xinli Lu

{"title":"Confirmation of Two Novel HIV-1 CRF01_AE/CRF07_BC Recombinant Forms Among Men Who Have Sex with Men in Hebei, China.","authors":"Yapeng Guan, Jun Wang, Meng Liu, Xinli Lu","doi":"10.1089/aid.2024.0104","DOIUrl":"10.1089/aid.2024.0104","url":null,"abstract":"Acquired immune deficiency syndrome caused by human immunodeficiency virus (HIV) is a serious infectious disease because of its' high genetic variability. Nowadays, homosexual contact has become the most predominant transmission route in Hebei province, China, leading to the emergence of novel HIV-1 recombinant forms. The neighbor-joining (N-J) phylogenetic trees were constructed using MEGA 6.0 in order to identify the subtypes of H22063 and H22144. Recombination breakpoints were identified using online resources jpHMM, RIP 3.0, and Simplot 3.5.1. In this study, we identified two novel HIV-1 unique recombinant forms (URFs) _0107 from three men who have sex with men in Hebei province, including H22063 and H22144. The near full-length genome analysis showed H22063 has seven gene recombination sub-regions, including three subtype CRF07_BC gene fragments inserted into the CRF01_AE backbone. H22144 has nine gene recombination sub-regions, including four subtype CRF07_BC gene fragments inserted into the CRF01_AE backbone. This study confirms the emergence of novel recombinant forms and suggests we should strengthen the monitoring of novel HIV recombinant forms in order to deal with the complex HIV-1 epidemiological trend in Hebei province, China.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"220-224"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a Novel HIV-1 Second-Generation Circulating Recombinant Form (CRF117_0107) in China. 一种新型HIV-1第二代循环重组病毒（CRF117_0107）在中国的鉴定

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-01-17 DOI: 10.1089/aid.2024.0106

Xiaorui Wang, Bo Zhu, Hanping Li, Jingwan Han, Xiaolin Wang, Lei Jia, Bohan Zhang, Jingyun Li, Yongjian Liu, Hongling Wen, Lin Li

{"title":"Identification of a Novel HIV-1 Second-Generation Circulating Recombinant Form (CRF117_0107) in China.","authors":"Xiaorui Wang, Bo Zhu, Hanping Li, Jingwan Han, Xiaolin Wang, Lei Jia, Bohan Zhang, Jingyun Li, Yongjian Liu, Hongling Wen, Lin Li","doi":"10.1089/aid.2024.0106","DOIUrl":"10.1089/aid.2024.0106","url":null,"abstract":"Under the background of the main epidemic HIV strains (CRF01_AE and CRF07_BC) co-circulation in China, more HIV second-generation recombinant (SGR) strains with CRF01_AE and CRF07_BC as the backbone were also emerging. In this study, we characterize a novel HIV-1 second-generation circulating recombinant form (CRF117_0107) consisting of CRF01_AE and CRF07_BC fragments from three epidemiologically unrelated individuals infected with HIV-1. One near full-length genome (NFLG) sequence was amplified, sequenced, and spliced in two halves using RNA extracted from the plasma of a homosexual in Shenzhen, Guangdong Province. Two other NFLG sequences were obtained from the Los Alamos HIV Sequence Database under accession numbers KY201177 and MK397789, which were isolated from men who have sex with men (MSM) in Guangdong Province and Zhejiang Province, respectively. Phylogenetic analysis revealed that these NFLG sequences formed a monophyletic cluster with a high bootstrap value of 1.0. Recombination analysis demonstrated that the genome of CRF117_0107 was separated into three segments by two breakpoints. Further subregional phylogenetic analysis was performed that showed segment I+III (790-5990nt, 8295-9412nt) of CRF117_0107 originated from the CRF07_BC cluster, and Segment I+III (5991-8294nt) originated from the CRF01_AE cluster. The appearance of CRF117_0107 further highlights that HIV-1 SGR strains containing CRF01_AE and CRF07_BC will be generated more frequently and will most likely be more conducive to accelerating the spread of HIV in China. This study suggested it's essential to monitor HIV-1 second-generation CRFs among high-risk populations such as MSM for the epidemic and evolution dynamics of HIV-1 in China.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"211-215"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142998541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient Viremia Among People with HIV Receiving Injectable Cabotegravir Plus Rilpivirine.

IF 1.5 4区医学

AIDS research and human retroviruses Pub Date : 2025-04-01 Epub Date: 2025-02-03 DOI: 10.1089/aid.2024.0083

Lauren F O'Connor, Wei Li Adeline Koay, Justin Unternaher, Morgan Byrne, Anne K Monroe, Alan Greenberg, Amanda D Castel, Natella Rakhmanina

{"title":"Transient Viremia Among People with HIV Receiving Injectable Cabotegravir Plus Rilpivirine.","authors":"Lauren F O'Connor, Wei Li Adeline Koay, Justin Unternaher, Morgan Byrne, Anne K Monroe, Alan Greenberg, Amanda D Castel, Natella Rakhmanina","doi":"10.1089/aid.2024.0083","DOIUrl":"10.1089/aid.2024.0083","url":null,"abstract":"Long-acting injectable (LAI) cabotegravir/rilpivirine (CAB/RPV) provides an effective treatment option for people with HIV (PWH). Studies suggest that PWH on LAI CAB/RPV may experience isolated episodes of transient viremia (HIV RNA > 20 copies/mL) defined as virologic blips (VB). The risk factors for VB in PWH receiving LAI CAB/RPV are limited. We aimed to describe a cohort of PWH on LAI CAB/RPV and evaluate risk factors and time to VB following LAI CAB/RPV initiation. We obtained DC Cohort data from PWH who initiated LAI CAB/RPV prior to July 2023 and used Kaplan-Meier curves and Cox proportional hazards models to evaluate the association between participant demographics, HIV clinical factors, and time to VB. Among 98 PWH who initiated LAI CAB/RPV, 9 (9.2%) experienced at least one VB (median HIV RNA = 50 copies/mL; ranges 30-12,000 copies/mL) during a median follow-up period of five months (IQR: 2-10). The median CD4 count among PWH was 754 cells/µL (IQR: 598, 980) at the time of LAI CAB/RPV initiation. Having a high CD4 (≥ 500 cells/μL) at LAI CAB/RPV initiation was significantly associated with a lower hazard for VB when compared to baseline CD4 < 200 cells/µL [hazard ratios (HR): 0.15 [95% confidence intervals (CI): 0.03, 0.77]; aHR: 0.07 (95% CI: 0.01, 0.50); log-rank p = .026]. No other characteristics were significantly associated with time to VB, and no participants experienced virologic failure. Considerations for baseline CD4 may be important when initiating a patient on LAI CAB/RPV, and future studies will help evaluate the VB occurrence and associated factors among PWH.","PeriodicalId":7544,"journal":{"name":"AIDS research and human retroviruses","volume":" ","pages":"181-188"},"PeriodicalIF":1.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0