Acta pathologica et microbiologica Scandinavica. Section C, Immunology最新文献_第6页

The binding of protein A of immunoglobulin G and of Fab and Fc fragments. 免疫球蛋白G的蛋白A与Fab和Fc片段的结合。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

C Endresen

引用次数: 0

Cytological events in allo-stimulated lymphocytes triggered by exposure to stimulatory alloantigens. II. Changes in the areal density of cytoplasmic vacuoles and in the subcellular localization of acid phosphatase. 暴露于刺激性同种异体抗原引发的同种异体刺激淋巴细胞的细胞学事件。2细胞质空泡面密度和酸性磷酸酶亚细胞定位的变化。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

P B Poulsen, L H Nielsen

{"title":"Cytological events in allo-stimulated lymphocytes triggered by exposure to stimulatory alloantigens. II. Changes in the areal density of cytoplasmic vacuoles and in the subcellular localization of acid phosphatase.","authors":"P B Poulsen, L H Nielsen","doi":"","DOIUrl":"","url":null,"abstract":"H-2b lymphocytes were sensitized against H-2d alloantigens by lymphocyte culture reaction and incubated with H-2d mastocytoma cells. The interaction between lymphoid cells and mastocytoma cells was stopped by fixation with glutaraldehyde. The areal density of the cytoplasmic vacuoles as well as the subcellular localization of acid phosphatase in lymphocytes were examined by electron microscopy. Two populations of lymphocytes were observed, small lymphocytes with heterochromatic nuclei and larger lymphocytes (lymphoblasts) with euchromatic nuclei. Only the lymphoblasts showed change following interaction with target cells. The vacuole area in percent of cytoplasmic area (vacuole areal density) of sensitized lymphoblasts increased during the first 30 minutes and from the third to fourth hour of interaction with target cells. Acid phosphatase staining was observed in the Golgi apparatus of the lymphoblasts after 30 minutes of interaction. Multivesicular bodies showed acid phosphatase staining within 20 minutes of interaction with target cells. After 20 minutes of interaction, phagosomes containing myelin figures were formed. These phagosomes also showed acid phosphatase staining and during the next hours of interaction their number increased over the number of multivesicular bodies.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87C 3","pages":"203-11"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11376386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of human monocytes by mediators from lymphocytes stimulated with Corynebacterium parvum. 细小棒状杆菌刺激淋巴细胞的介质活化人单核细胞。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

J Hammerström, G Unsgaard, J Lamvik

引用次数: 0

Examination of parotid saliva for antibodies reacting with Streptococcus mutans, lipoteichoic acid and peptidoglycan by the enzyme-linked immunosorbent assay. 用酶联免疫吸附法检测腮腺唾液与变形链球菌、脂磷胆酸和肽聚糖反应的抗体。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

D Bratthall, A Carlén, K W Knox, A J Wicken

引用次数: 0

The human mixed lymphocyte reaction responder and stimulator capacities of highly purified T- and non-T cells and the role of monocytes. 人混合淋巴细胞反应反应和刺激能力的高度纯化的T和非T细胞和单核细胞的作用。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

P Hokland, I Heron

引用次数: 0

Lymphocytes from adenoid vegetations: proliferative responses in vitro as compared to blood lymphocytes. 来自腺样体植物的淋巴细胞:与血液淋巴细胞相比，体外增殖反应。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

H H Mogensen, K I Meistrup-Larsen, V Andersen

引用次数: 0

Demonstration of the non-identity between the Fc receptor for human IgG from group A streptococci type 15 and M protein, peptidoglycan and the group specific carbohydrate. A组链球菌15型人IgG Fc受体与M蛋白、肽聚糖和组特异性碳水化合物的不同一性证明。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

P Christensen, A Grubb, R Grubb, G Samuelsson, C Schalén, M L Svensson

{"title":"Demonstration of the non-identity between the Fc receptor for human IgG from group A streptococci type 15 and M protein, peptidoglycan and the group specific carbohydrate.","authors":"P Christensen, A Grubb, R Grubb, G Samuelsson, C Schalén, M L Svensson","doi":"","DOIUrl":"","url":null,"abstract":"After electrophoresis of an alkaline extract of type 15 group A streptococci, three main precipitation lines were obtained in diffusion experiments against commercial human polyclonal IgG (lines 1, 2 and 3). Nineteen of 23 sera (83%) from apparently healthy human individuals gave line 3, while 6 of them (26%) gave line 1. The sera giving line 1 did also give line 3. Line 2 was obtained with 2 sera only, also giving lines 1 and 3. Line 3 was caused by a streptococcal Fc-receptor for human IgG, since the line could be displaced by addition of Fc-fragments, but not Fab-fragments of pooled human IgG. Line 1 was shown to be different from line 3, since (1) line 1 was suppressed in contrast to line 3 on absorption of a human serum or commercial polyclonal human IgG with S. aureus; and (2), line 1 was suppressed by Fab-fragments but not Fc-fragments of polyclonal human IgG. Line 2 could be inhibited by addition of peptidoglycan to commercial polyclonal human IgG or a human serum investigated. Another line, 4, obtained in diffusion experiments involving electrophoretically separated alkaline extract of type 15 group A streptococci was type-specific as shown by rabbit antisera to streptococci type M1, M8, M15, and T44, and disappeared on trypsinization of the extract. The component responsible for line 4 in the streptococcal extract, judged to be type-specific M protein, had a mobility different from the component responsible for line 3 in electrophoresis.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87C 3","pages":"257-61"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11591309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractionation of connective-tissue-activating factors from the culture medium of silica-treated macrophages. 二氧化硅处理巨噬细胞培养基中结缔组织活化因子的分离。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

M Aalto, E Kulonen

{"title":"Fractionation of connective-tissue-activating factors from the culture medium of silica-treated macrophages.","authors":"M Aalto, E Kulonen","doi":"","DOIUrl":"","url":null,"abstract":"The medium of cultured, SiO2-treated peritoneal macrophages contained a factor which enhances the incorporation of labelled proline to collagen and other proteins in granulation tissue slices, cells and polysomes. Simultaneously, the activity of alkaline RNase in the whole medium was decreased in comparison with the corresponding control. Polyvinylpyridine-N-oxide, PVNO, protected the macrophages against SiO2. Latex-particles and E. coli lipopolysaccharide decreased the RNase activity in the macrophage medium, but unlike SiO2 did not cause liberation of the collagen synthesis-stimulating factor. Fractionation of the medium by gel filtration chromatography showed the SiO2-pretreatment to have caused a very significant decrease in the aggregation state of RNase. The fraction from gel filtration chromatography that contained the SiO2-liberated factor stimulating collagen synthesis also contained the disaggregated RNase. There was no RNase-activity in the control sample. A homogenous protein (mol. wt. 14,300) was isolated with repeated gel filtrations from the medium of silica-treated macrophages. It increased the incorporation of 3H proline and 3H thymidine into cultured granuloma cells.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87C 3","pages":"241-50"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11440156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A. 猪IgG酶解片段的分离及其对葡萄球菌蛋白A的反应性研究。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

C Endresen

{"title":"Isolation of enzymatically derived fragments of porcine IgG and an examination of their reactivity against staphylococcal protein A.","authors":"C Endresen","doi":"","DOIUrl":"","url":null,"abstract":"Papain digestion of porcine IgG in the absence of cysteine resulted in a rather poor yield of fragments (less than 5 per cent). In the presence of cysteine, 70 to 80 per cent of the IgG was degradated in 4 h. Fragments with molecular weight of about 100,000 and 50,000 were separated by gel filtration. The minor fraction (mol. wt. 100,000) most probably consisted of F(c)2 fragments. Fab/c fragments with both Fc and Fab determinants, and also probably some F(ab)2-like fragments. The F(c)2 fragments appeared to be a dimer of Fc stabilized by disulphide bonds. The second main fraction (mol. wt. 50,000) contained Fc and Fab fragments. Mild reduction of the Fc fragments resulted in Fc subfragments of different sizes, thus indicating that papain cleavages had occurred on different spots in the Fc chain. Non-reduced Fc fragments therefore seem to consist of several Fc subfragments stabilized by disulphide bonds. The protein A reactivity of the isolated Fc fragments were rather low compared to the reactivity of intact IgG, respectively 5--15 and 90 per cent. In addition, protein A reactive Fab fragments were isolated from normal porcine IgG.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"87C 3","pages":"177-83"},"PeriodicalIF":0.0,"publicationDate":"1979-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11333730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron microscopy of treponemes subjected to the Treponema pallidum immobilization (TPI) test. I. Comparison of immunoimmobilized cells and control cells. 梅毒螺旋体固定(TPI)试验的螺旋体电子显微镜。1 .免疫固定细胞与对照细胞的比较。

Acta pathologica et microbiologica Scandinavica. Section C, Immunology Pub Date : 1979-06-01

K Hovind-Hougen, H A Nielsen, A Birch-Andersen

引用次数: 0