Acta pathologica et microbiologica Scandinavica. Section B, Microbiology最新文献

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Survival of anaerobic bacteria during transportation. 1. Experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen. 厌氧细菌在运输过程中的存活。1. 用二氧化碳和氮气冲洗排气效果的实验研究。
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1976.TB01900.X
T. Justesen, M. L. Neilsen
{"title":"Survival of anaerobic bacteria during transportation. 1. Experimental investigations on the effect of evacuation of atmospheric air by flushing with carbon dioxide and nitrogen.","authors":"T. Justesen, M. L. Neilsen","doi":"10.1111/J.1699-0463.1976.TB01900.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01900.X","url":null,"abstract":"The effect of evacuation of atmospheric air during transportation on recovery of anaerobic bacteria was investigated. Evacuation of atmospheric air from glass tubes by flushing with pure carbon dioxide lowered the content of oxygen to about 0.4 per cent. Three B. fragilis strains and one strain of Fusobacterium mortiferum and of Peptostreptococcus anaerobius were investigated. Bacterial recovery was determined one hour and 24 hours after evacuation of atmospheric air by pure carbon dioxide and pure nitrogen, was compared to bacterial recovery from samples transported with free access to atmospheric air. Evacuation by pure carbon dioxide significantly improved the recovery of one B. fragilis strain after 24 hours of transportation and significantly impaired the recovery of Peptostreptococcus anaerobius after one hour of transportation, while evacuation by pure nitrogen significantly improved the recovery of Peptostreptococcus anaerobius after 24 hours of transportation. In all other cases, however, no statistically significant effect on bacterial recovery was found.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"17 1","pages":"51-6"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74035664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
HUMAN INTERFERON AND CELL GROWTH INHIBITION 人干扰素与细胞生长抑制
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1977.TB01674.X
H. Dahl
{"title":"HUMAN INTERFERON AND CELL GROWTH INHIBITION","authors":"H. Dahl","doi":"10.1111/J.1699-0463.1977.TB01674.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1977.TB01674.X","url":null,"abstract":"Human leukocyte interferon (IF) inhibits the growth rate of homologous cells in culture. The growth inhibitory component, formerly separated from the antiviral component of human leukocyte IF by adsorption chromatography on albumin-agarose, is investigated. The properties of the component are compared with the accepted characteristics of IF. The growth inhibition is caused by a small molecule, unstable to pH 2 treatment with HCl, but stable to trichloro-acetic acid (TCA) and to proteolytic enzymes. The isolated growth inhibitor seems to be a dimer of molecular weight (mol wt.)˜2300. It is activated by heat treatment and lacks species specificity. The discrepancies in the properties of the growth inhibitor before before and after separation from IF are discussed.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"26 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85283140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic mapping of the K1 and K4 antigens (L) of Escherichia coli. Non-allelism of K(L) antigens with K antigens of O8:K27(A), O8:K8(L) and O9:K57(B).
Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1976.TB01914.X
I. Orskov, V. Sharma, F. Orskov
{"title":"Genetic mapping of the K1 and K4 antigens (L) of Escherichia coli. Non-allelism of K(L) antigens with K antigens of O8:K27(A), O8:K8(L) and O9:K57(B).","authors":"I. Orskov, V. Sharma, F. Orskov","doi":"10.1111/J.1699-0463.1976.TB01914.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01914.X","url":null,"abstract":"Escherichia donor strains having antigen K1(L) or K4(L) transfer these K antigens to recipient cells at a genetic locus (kps A) similar to that of K10(L) and K54(L) linked to ser A. In crosses between the K10 donor strain and recipient strains O8:K8(L), O8:K27(A) and O9:K57(B) all recombinants which inherit donor K antigen also inherit K antigen of recipient. This result is interpreted as non-allelism between donor and recipient K antigens, and it is assumed that the structure of all polysaccharide K antigens of strains having O antigens O8 or O9, whether termed L, A, or B, are controlled by genes which differ in their location on the chromosome from genes controlling polysaccharide K antigens associated with most other O antigens.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"27 1","pages":"125-31"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82943900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 26
Effect of chlorpromazine on the ultrastructure of Staphylococcus aureus. 氯丙嗪对金黄色葡萄球菌超微结构的影响。
J E Kristiansen, J Blom
{"title":"Effect of chlorpromazine on the ultrastructure of Staphylococcus aureus.","authors":"J E Kristiansen,&nbsp;J Blom","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructure of Staphylococcus aureus grown on plates containing chlorpromazine (CPZ) in concentrations up to a bacteriostatic level (50 micrograms/ml) was studied. High concentrations of CPZ induced large mesosome-like structures and asymmetrical cell divisions in a considerable number of cells.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"399-405"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18346476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A sandwich ELISA method for detection of Staphylococcus aureus enterotoxins. 夹心ELISA法检测金黄色葡萄球菌肠毒素。
B P Berdal, O Olsvik, T Omland
{"title":"A sandwich ELISA method for detection of Staphylococcus aureus enterotoxins.","authors":"B P Berdal,&nbsp;O Olsvik,&nbsp;T Omland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Staphylococcus aureus enterotoxins A, B, and C, were detected by means of a four-layer sandwich ELISA method. With toxin produced in broth supernatants, this ELISA method had a detection limit of 0.5 ng enterotoxin per ml. Conjoint with enterotoxin, S. aureus most often produces protein A. The protein A will interfere and produce false positive reactions in a sandwich ELISA, by binding nonspecifically the IgG in different layers, simulating the immunospecific toxin binding. With rabbit IgG coupled to Sepharose CL-4B gel, 99% of protein A could be removed from solutions, bringing the level under the interfering limit in the ELISA.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"411-5"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18346477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enterotoxin production by Staphylococcus aureus related to the origin of the strains. 产肠毒素的金黄色葡萄球菌与菌株的来源有关。
O Olsvik, B P Berdal, K Fossum, T Omland
{"title":"Enterotoxin production by Staphylococcus aureus related to the origin of the strains.","authors":"O Olsvik,&nbsp;B P Berdal,&nbsp;K Fossum,&nbsp;T Omland","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The production of enterotoxins by strains of Staphylococcus aureus of human and animal origin seems to be common. 104 out of 170 strains (61%) produced one or more of the A, B, and C enterotoxins. Strains from cow and milk often produced enterotoxin C, and enterotoxin A producing strains were mainly isolated from dogs. Human food poisoning seemed in our material to be induced by enterotoxin A producing strains.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"423-6"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18346480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IgG-Fc-receptors in T-type 12 group A streptococci from clinical specimens: absence from M-type 12 and presence in M-type 22. 临床标本中t - 12型A组链球菌igg - fc受体:m - 12型缺失,m - 22型存在。
L A Burova, P Christensen, R Grubb, I A Krasilnikov, G Samuelsson, C Schalén, M L Svensson, U Zätterström
{"title":"IgG-Fc-receptors in T-type 12 group A streptococci from clinical specimens: absence from M-type 12 and presence in M-type 22.","authors":"L A Burova,&nbsp;P Christensen,&nbsp;R Grubb,&nbsp;I A Krasilnikov,&nbsp;G Samuelsson,&nbsp;C Schalén,&nbsp;M L Svensson,&nbsp;U Zätterström","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eighty-eight T-type 12 group A streptococcal strains were randomly selected from routine specimens in two epidemiologically unrelated districts. All of 26 M-type 12 strains lacked IgA and IgG Fc-receptors, whereas all 32 M-type 22 strains had IgG Fc-receptors and 20 of them also receptors for IgA Fc. The remaining strains were not further M-typed but 18 of these 30 strains exhibited receptors for IgG and 13 for IgA.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"433-5"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18085736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The use of Staphylococcus aureus rich in protein A in the detection of herpes simplex virus antigens. 利用富含蛋白A的金黄色葡萄球菌检测单纯疱疹病毒抗原。
S C Mogensen, T Dishon
{"title":"The use of Staphylococcus aureus rich in protein A in the detection of herpes simplex virus antigens.","authors":"S C Mogensen,&nbsp;T Dishon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Herpes simplex virus (HSV) type 1 antigens were detected in infected human embryonic lung cells with the aid of specific antiserum and Staphylococcus aureus rich in protein A. When such staphylococci carrying specific anti-HSV IgG on their surface were interacted with various suspension of virus, a reduction in the initial virus titre of about 65% was obtained. However, no direct coagglutination was observed between cell-free supernatants of HSV or HSV-infected cells and sensitized staphylococci. When monolayers or suspended cells infected with the virus were treated with dilutions of specific anti-HSV antiserum followed by non-sensitized staphylococci (indirect method), an \"aureola\" of the bacteria was detected around the cells expressing the viral antigens. A similar picture was observed when infected cells were interacted directly with sensitized staphylococci. Viral antigens were detected already 12 hours post infection, well before the appearance of cytopathic effect. The sensitivity of the indirect method was found to be higher than that of the direct one and dependent on the multiplicity of infection and the serum dilution used. The method is proposed as a rapid means of identifying viral antigens in diagnostic and experimental virology.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"427-32"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17341189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Stereo-isomeric dissociation of the antibacterial and the neuroleptic effect of clopenthixol. 氯戊硫醇的立体异构体解离抑菌及抗精神病作用。
J E Kristiansen, I Mortensen
{"title":"Stereo-isomeric dissociation of the antibacterial and the neuroleptic effect of clopenthixol.","authors":"J E Kristiansen,&nbsp;I Mortensen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Measurement of the IC50 of cis(Z)-clopenthixol and trans(E)-clopenthixol on 188 bacterial strains from human clinical specimens shows that the antibacterial activity of clopenthixol is exerted by both isomerical components and trans(E)-clopenthixol is the most active antibiotic of the two drugs against the sensitive strains. It is known that the trans(E)-clopenthixol isomere is without neuroleptic effect. The possibility of creating new antibiotics by e.g. steric alteration of neuroleptical agents is stressed. These drugs have different antibiotic patterns from those of classical antibiotics. It seems particularly promising that Pseudomonas aeruginosa is sensitive to these drugs.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"437-8"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17187689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toxinogenicity of clinical isolates of coagulase-negative staphylococci towards various animal cells. 凝固酶阴性葡萄球菌临床分离株对多种动物细胞的毒性研究。
C G Gemmell, M Thelestam
{"title":"Toxinogenicity of clinical isolates of coagulase-negative staphylococci towards various animal cells.","authors":"C G Gemmell,&nbsp;M Thelestam","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The toxinogenicity of fifty coagulase-negative staphylococci isolated from various clinical syndromes was compared, using lysis of human erythrocytes, leakage of a radio-active marker from human embryonic lung fibroblasts by culture filtrates, and direct cytotoxicity of growing bacteria towards mouse skin fibroblasts in an agar overlay assay. Good correlation was obtained between those strains which elaborated greater than or equal to 16 HU/ml delta-toxin in culture, measurable also in terms of radioactive nucleotide leakage from tissue culture cells and those strains which caused a direct cytotoxicity effect in the colony overlay test. delta-toxin was implicated in the genesis of such cellular damage. Speciation of the coagulase-negative staphylococci revealed that isolates identified as S. epidermidis, S. saprophyticus and S. haemolyticus were most active in each of these tests. The colony overlay technique is suggested as being a potential screening assay for toxinogenic coagulase-negative staphylococci associated with clinical infections.</p>","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 6","pages":"417-21"},"PeriodicalIF":0.0,"publicationDate":"1981-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18346479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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