Acta pathologica et microbiologica Scandinavica. Section B, Microbiology最新文献_第4页

Recency of Toxoplasma gondii infections correlated with results obtained in dye test and enzyme-linked immunosorbent assay. 刚地弓形虫感染的近发性与染色试验和酶联免疫吸附试验结果相关。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-12-01

E K Pettersen

引用次数: 0

Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens. 呼吸道合胞病毒感染的免疫荧光快速诊断:鼻咽吸吸标本制备的简化程序。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-08-01

G Anestad, O R Maehle

引用次数: 0

Susceptibility of continuous lines of monkey kidney cells to influenza and parainfluenza viruses in the presence of trypsin. 在胰蛋白酶存在下，猴子肾细胞连续系对流感和副流感病毒的敏感性。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-06-01

I Orstavik

{"title":"Susceptibility of continuous lines of monkey kidney cells to influenza and parainfluenza viruses in the presence of trypsin.","authors":"I Orstavik","doi":"","DOIUrl":"","url":null,"abstract":"LLC-MK2, GMK AH-1, BSC-1, and Vero cells were compared in titrations of recent isolates and laboratory strains of influenza A and B and parainfluenza types 1, 2, and 3 viruses. About the same titres, as determined by haemadsorption in cell cultures, were obtained in LLC-MK2, GMK AH-1, and BSC-1 cells when trypsin had been added to the medium, whereas the Vero cells were less sensitive to the influenza virus strains tested. Virus titres were usually low in the absence of trypsin. A laboratory strain of parainfluenza 2 virus reached about the same titres in medium without as in medium with trypsin, possibly owing to prior adaptation by passages in Vero cells. Comparative titrations of influenza A, and parainfluenza 1 and 3 viruses suggested the same susceptibility of LLC-MK2 cells with trypsin as of primary monkey kidney cells. Re-isolation experiments from 38 clinical specimens showed LLC-MK2 cells to be as efficient as primary monkey kidney cells for isolation of influenza and parainfluenza viruses, whereas the susceptibility of the other cell lines to clinical material has not yet been tested on a larger scale. It is concluded that a continuous line of monkey kidney cell culture may be acceptable as an alternative to primary monkey kidney cells for the isolation of influenza and parainfluenza viruses from patients.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 3","pages":"179-83"},"PeriodicalIF":0.0,"publicationDate":"1981-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17336268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracted protective antigen of Bordetella pertussis. I. Preparation and properties of the solubilized surface of components. 提取百日咳杆菌保护性抗原。一、组分增溶表面的制备及性能。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-04-01

T B Helting, F Blackkolb

{"title":"Extracted protective antigen of Bordetella pertussis. I. Preparation and properties of the solubilized surface of components.","authors":"T B Helting, F Blackkolb","doi":"","DOIUrl":"","url":null,"abstract":"Bordetella pertussis microorganisms were treated with several extracting agents followed by ultracentrifugation to remove particulate matter. Analysis of the resulting supernatants by SDS gel electrophoresis showed one major component after simple salt extraction, and much more complex, although consistent pattern following detergent treatment. The yield of the solubilized protein in detergent extracts exceeded by far the values recorded for salt extracts. In order to prevent irreversible precipitation of the solubilized proteins upon removal of the denaturing agent, a novel procedure was developed. After extraction with urea-salt, the solubilized material was absorbed on a mineral carrier prior to the separation of the denaturing agent. The resulting absorbed vaccine was highly potent in the mouse-protection test, whereas the toxic reactions, elicited upon injection into experimental animals, were reduced in the comparison to the starting material. This diminished reactogenic potential was accompanied by the partial loss of the leukocytosis-promiting factor, whose activity was greatly diminished by urea-salt at alkaline pH-values. The procedure described may be applied to large-scale processing of Bordetella persussis microorganisms. Clinical trials now in progress should confirm or rebut the thesis that increased tolerability of the product, inferred from animal experiments, is reflected by fewer adverse reactions in humans. In the former case, the detergent extract vaccine may constitute a realistic alternative to conventional whole-cell vaccines against whooping-cough.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 2","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"1981-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17328621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic transformation of Streptococcus sanguis. Further studies on the production and isolation of the competence factor. 血链球菌的遗传转化。能力因子的产生与分离的进一步研究。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-04-01

P Gaustad, J Eriksen

引用次数: 0

Extracted protective antigen of Bordetella pertussis. I. Preparation and properties of the solubilized surface of components. 提取百日咳杆菌保护性抗原。一、组分增溶表面的制备及性能。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-03-01 DOI: 10.1111/J.1699-0463.1981.TB00159_89B.X

T. Helting, F. Blackkolb

{"title":"Extracted protective antigen of Bordetella pertussis. I. Preparation and properties of the solubilized surface of components.","authors":"T. Helting, F. Blackkolb","doi":"10.1111/J.1699-0463.1981.TB00159_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00159_89B.X","url":null,"abstract":"Bordetella pertussis microorganisms were treated with several extracting agents followed by ultracentrifugation to remove particulate matter. Analysis of the resulting supernatants by SDS gel electrophoresis showed one major component after simple salt extraction, and much more complex, although consistent pattern following detergent treatment. The yield of the solubilized protein in detergent extracts exceeded by far the values recorded for salt extracts. In order to prevent irreversible precipitation of the solubilized proteins upon removal of the denaturing agent, a novel procedure was developed. After extraction with urea-salt, the solubilized material was absorbed on a mineral carrier prior to the separation of the denaturing agent. The resulting absorbed vaccine was highly potent in the mouse-protection test, whereas the toxic reactions, elicited upon injection into experimental animals, were reduced in the comparison to the starting material. This diminished reactogenic potential was accompanied by the partial loss of the leukocytosis-promiting factor, whose activity was greatly diminished by urea-salt at alkaline pH-values. The procedure described may be applied to large-scale processing of Bordetella persussis microorganisms. Clinical trials now in progress should confirm or rebut the thesis that increased tolerability of the product, inferred from animal experiments, is reflected by fewer adverse reactions in humans. In the former case, the detergent extract vaccine may constitute a realistic alternative to conventional whole-cell vaccines against whooping-cough.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"69 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"1981-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90633222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparison of serological tests for detection of Mycoplasma hominis antibodies in female Grivet monkeys with experimentally induced salpingitis. 实验性输卵管炎雌性灰尾猴人支原体抗体血清学检测的比较。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-02-01

B R Møller

{"title":"Comparison of serological tests for detection of Mycoplasma hominis antibodies in female Grivet monkeys with experimentally induced salpingitis.","authors":"B R Møller","doi":"","DOIUrl":"","url":null,"abstract":"Four serological tests, viz. indirect hemagglutination (IHA), metabolism inhibition (MI), immunofluorescence (IMF), and a modification of the growth-inhibition (GI) test have been compared for the detection of antibodies against Mycoplasma hominis in female grivet monkeys with experimentally induced pelvic inflammatory disease. Moreover, cold hemagglutinins (CHA), and immunoglobulins, M, G, and A have been determined. The IHA test was found to be superior to the other methods used. The antibodies were present in all inoculated monkeys two weeks after the inoculation and a maximum in titre occurred one week later. Antibodies detected by the modified GI test occurred in all monkeys inoculated with the organism together with the IHA antibodies, but the maximum in titre was less marked. The IMF test was less sensitive than the GI test; the antibodies generally occurred later and disappeared faster. Using the MI test, no antibodies could be detected in any of the monkeys during the experimental period and no CHA antibodies were found in any of the sera. IgM rose earlier and declined more rapidly than IgG. It is concluded that the IHA test is most suitable for the measurement of serum antibodies caused by infection with M. hominis in grivet monkeys. The modified GI test, although less sensitive, may also be useful because of its simplicity in performance.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"1981-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18066602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A modified isolation technique for Chlamydia psittaci in L-cells treated with cycloheximide and glucocorticoid. 环己亚胺和糖皮质激素处理l细胞分离鹦鹉热衣原体的改进技术。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1981-02-01

L Rønsholt

{"title":"A modified isolation technique for Chlamydia psittaci in L-cells treated with cycloheximide and glucocorticoid.","authors":"L Rønsholt","doi":"","DOIUrl":"","url":null,"abstract":"Various auxiliary treatments of L-cells employed for the isolation and cultivation of C. psittaci were investigated in order to develop an improved method for the detection of the agent, in addition to the aid obtained by centrifugation and cycloheximide treatment. Glucocorticoid treatment increased the observed number of inclusions considerably through a preservative effect on host cells and enhanced an spontaneous re-infection. Besides, the hormone made the scanning of cell culture for inclusions more convenient through an altered cell morphology. This method was tested with two extreme species types that differed as regards cytopathogenicity and growth rate. The length of the cultivation period was of great importance for the diagnostic result. Especially the cytopathogenic agent-type influenced the optimal time of cell culture fixation, which was situated around 48 or 88 hours post infection (h p.i.). Owing to the cytotoxicity of field samples (milk secretion), the cell culture technique (48 h p.i.) was less sensitive compared to the conventional isolation method in embryonated eggs. However, a different sampling technique improved the result, and simultaneous use of the secondary multiplication cycle of chlamydia (88 h p.i.) makes the less cumbersome cell culture technic recommendable.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 1","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"1981-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18068468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibodies to the strain-specific and cross-reactive determinants of the haemagglutinin of influenza H3N2 virus. 3. Selection of antigenic variations in vitro and in vivo. H3N2流感病毒血凝素毒株特异性和交叉反应性决定因子抗体。3.体外和体内抗原变异的选择。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1980-12-01

L R Haaheim

引用次数: 0

Studies on the pathogenicity of Yersinia enterocolitica and Y. enterocolitica-like bacteria. 1. Enterotoxin production at 22 degrees C and 37 degrees C by environmental and human isolates from Scandinavia. 小肠结肠炎耶尔森氏菌和小肠结肠炎样耶尔森氏菌致病性的研究。1. 来自斯堪的纳维亚半岛的环境分离株和人类分离株在22℃和37℃条件下产生的肠毒素。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 1980-10-01

G Kapperud

引用次数: 0