Acta pathologica et microbiologica Scandinavica. Section B, Microbiology最新文献

A modified isolation technique for Chlamydia psittaci in L-cells treated with cycloheximide and glucocorticoid. 环己亚胺和糖皮质激素处理l细胞分离鹦鹉热衣原体的改进技术。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-19 DOI: 10.1111/J.1699-0463.1981.TB00146_89B.X

L. Rönsholt

{"title":"A modified isolation technique for Chlamydia psittaci in L-cells treated with cycloheximide and glucocorticoid.","authors":"L. Rönsholt","doi":"10.1111/J.1699-0463.1981.TB00146_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00146_89B.X","url":null,"abstract":"Various auxiliary treatments of L-cells employed for the isolation and cultivation of C. psittaci were investigated in order to develop an improved method for the detection of the agent, in addition to the aid obtained by centrifugation and cycloheximide treatment. Glucocorticoid treatment increased the observed number of inclusions considerably through a preservative effect on host cells and enhanced an spontaneous re-infection. Besides, the hormone made the scanning of cell culture for inclusions more convenient through an altered cell morphology. This method was tested with two extreme species types that differed as regards cytopathogenicity and growth rate. The length of the cultivation period was of great importance for the diagnostic result. Especially the cytopathogenic agent-type influenced the optimal time of cell culture fixation, which was situated around 48 or 88 hours post infection (h p.i.). Owing to the cytotoxicity of field samples (milk secretion), the cell culture technique (48 h p.i.) was less sensitive compared to the conventional isolation method in embryonated eggs. However, a different sampling technique improved the result, and simultaneous use of the secondary multiplication cycle of chlamydia (88 h p.i.) makes the less cumbersome cell culture technic recommendable.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"22 1","pages":"13-23"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77819645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic transformation of Streptococcus sanguis. Further studies on the production and isolation of the competence factor. 血链球菌的遗传转化。能力因子的产生与分离的进一步研究。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-19 DOI: 10.1111/J.1699-0463.1981.TB00156_89B.X

P. Gaustad, J. Eriksen

引用次数: 0

Comparison of serological tests for detection of Mycoplasma hominis antibodies in female Grivet monkeys with experimentally induced salpingitis. 实验性输卵管炎雌性灰尾猴人支原体抗体血清学检测的比较。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-19 DOI: 10.1111/J.1699-0463.1981.TB00145_89B.X

Birger R. Møller

{"title":"Comparison of serological tests for detection of Mycoplasma hominis antibodies in female Grivet monkeys with experimentally induced salpingitis.","authors":"Birger R. Møller","doi":"10.1111/J.1699-0463.1981.TB00145_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00145_89B.X","url":null,"abstract":"Four serological tests, viz. indirect hemagglutination (IHA), metabolism inhibition (MI), immunofluorescence (IMF), and a modification of the growth-inhibition (GI) test have been compared for the detection of antibodies against Mycoplasma hominis in female grivet monkeys with experimentally induced pelvic inflammatory disease. Moreover, cold hemagglutinins (CHA), and immunoglobulins, M, G, and A have been determined. The IHA test was found to be superior to the other methods used. The antibodies were present in all inoculated monkeys two weeks after the inoculation and a maximum in titre occurred one week later. Antibodies detected by the modified GI test occurred in all monkeys inoculated with the organism together with the IHA antibodies, but the maximum in titre was less marked. The IMF test was less sensitive than the GI test; the antibodies generally occurred later and disappeared faster. Using the MI test, no antibodies could be detected in any of the monkeys during the experimental period and no CHA antibodies were found in any of the sera. IgM rose earlier and declined more rapidly than IgG. It is concluded that the IHA test is most suitable for the measurement of serum antibodies caused by infection with M. hominis in grivet monkeys. The modified GI test, although less sensitive, may also be useful because of its simplicity in performance.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"64 1","pages":"7-11"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90377742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Susceptibility of continuous lines of monkey kidney cells to influenza and parainfluenza viruses in the presence of trypsin. 在胰蛋白酶存在下，猴子肾细胞连续系对流感和副流感病毒的敏感性。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-19 DOI: 10.1111/J.1699-0463.1981.TB00173_89B.X

I. Ørstavik

{"title":"Susceptibility of continuous lines of monkey kidney cells to influenza and parainfluenza viruses in the presence of trypsin.","authors":"I. Ørstavik","doi":"10.1111/J.1699-0463.1981.TB00173_89B.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1981.TB00173_89B.X","url":null,"abstract":"LLC-MK2, GMK AH-1, BSC-1, and Vero cells were compared in titrations of recent isolates and laboratory strains of influenza A and B and parainfluenza types 1, 2, and 3 viruses. About the same titres, as determined by haemadsorption in cell cultures, were obtained in LLC-MK2, GMK AH-1, and BSC-1 cells when trypsin had been added to the medium, whereas the Vero cells were less sensitive to the influenza virus strains tested. Virus titres were usually low in the absence of trypsin. A laboratory strain of parainfluenza 2 virus reached about the same titres in medium without as in medium with trypsin, possibly owing to prior adaptation by passages in Vero cells. Comparative titrations of influenza A, and parainfluenza 1 and 3 viruses suggested the same susceptibility of LLC-MK2 cells with trypsin as of primary monkey kidney cells. Re-isolation experiments from 38 clinical specimens showed LLC-MK2 cells to be as efficient as primary monkey kidney cells for isolation of influenza and parainfluenza viruses, whereas the susceptibility of the other cell lines to clinical material has not yet been tested on a larger scale. It is concluded that a continuous line of monkey kidney cell culture may be acceptable as an alternative to primary monkey kidney cells for the isolation of influenza and parainfluenza viruses from patients.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"79 1","pages":"179-183"},"PeriodicalIF":0.0,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78693580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens. 呼吸道合胞病毒感染的免疫荧光快速诊断:鼻咽吸吸标本制备的简化程序。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-19 DOI: 10.1111/J.1699-0463.1981.TB00190_89B.X

Gabriel Ånestad, Osvald Ragnvald Mœhle

引用次数: 0

Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 1. Sendai virus haemolysis-inhibiting antibodies. 副粘病毒特异性溶血抑制抗体与血凝抑制抗体和神经氨酸酶抑制抗体的分离鉴定。1. 仙台病毒溶血抑制抗体。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1976.TB01965.X

C. Örvell

{"title":"Identification of paramyxovirus-specific haemolysis-inhibiting antibodies separate from haemagglutinating-inhibiting and neuraminidase-inhibiting antibodies. 1. Sendai virus haemolysis-inhibiting antibodies.","authors":"C. Örvell","doi":"10.1111/J.1699-0463.1976.TB01965.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01965.X","url":null,"abstract":"Egg-grown Sendai virus was used for preparation of rabbit hyperimmune sera directed against purified whole virus and pronasetreated projectionless virus particles. These sera and convalescent sera after natural Sendai infection in guinea pigs were studied in haemolysis-inhibition (HLI), haemagglutination-inhibition (HI) and neuraminidase-inhibition (NI) tests both before and after absorption with Tween 80-ether (TE) treated virus preparations. In addition, neutralization tests using the different sera were carried out. HI and NI antibodies and the major population of neutralizing antibodies in convalescent sera were removed by absorption with TE treated virus material without changing the titre of non-HI HLI antibodies. Rabbit hyperimmune sera directed against projectionless virus particles exhibited HLI antibody titres in marked excess of HI and NI antibody titres, whereas this was not found in sera against purified whole virus. In contrast, absorption of sera against projectionless particles eliminated HI antibodies without changing the titre of non-HI HLI antibodies. The protein composition of antigenic preparations used in absorption experiments and for preparation of sera was investigated by SDS-polyacryladmie-gel electrophoresis. TH treatment had no significant effect on the polypeptide pattern of Sendai virus. Pronase-treatment predominantly affected the two glycosylated proteins of Sendai virus. The larger glycoprotein was not detectable in pronasetreated projectionless virus particles, whereas the smaller glycoprotein was present in reduced quantities.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"89 1","pages":"441-450"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80917513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Radiorespirometric studies in genus Neisseria. 3. The catabolism of pyruvate and acetate. 奈瑟菌属的放射肺量学研究。丙酮酸和醋酸酯的分解代谢。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/j.1699-0463.1976.tb01894.x

E. Holten

引用次数: 16

FIVE NEW ESCHERICHIA COLI K ANTIGENS, K95, K96, K97, K98 AND K100 5种新的大肠杆菌k抗原k95、k96、k97、k98和k100

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1976.TB01947.X

I. Ørskov, F. Ørskov

引用次数: 1

The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. II. Comparison of the antibody response in man against thirteen O groups of Ps. aeruginosa. 采用定量免疫电泳方法对铜绿假单胞菌进行血清学分析。2人对铜绿假单胞菌13组O型抗体反应的比较。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1975.TB00109.X

N. Høiby

{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. II. Comparison of the antibody response in man against thirteen O groups of Ps. aeruginosa.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1975.TB00109.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1975.TB00109.X","url":null,"abstract":"The occurrence of antibodies against antigens prepared from strains representing 13 O groups of Pseudomonas aeruginosa and against a polyvalent Ps. aeruginosa antigen (St-Ag) has been investigated in sera from 100 patients. By means of fused rocket immunoelectrophoresis with intermediate gel it was found that the humoral immune response against Ps. aeruginosa resulting in precipitating antibodies will be detected by St-Ag as well as by any other of the antigen samples investigated. Six of the sera contained group-specific antibodies which were revealed by only one of the antigen samples used and not by St-Ag. These six sera were further studied by means of various quantitative immunoelectrophoretic methods using St-Ag as well as antigens prepared from the infecting Ps. aeruginosa strain in the patient concerned. In all six sera, only one extra precipitin could be detected using antigens prepared from the homologous strain instead of St-Ag. This extra precipitin corresponded presumably to group-specific O-antigens not included in St-Ag. In sera from patients, these group-specific antibodies were always accompanied by antibodies against antigens common to all strains of Ps. aeruginosa.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"136 1","pages":"328-34"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77461343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sorption of Streptococci to glass: Effects of macromolecular solutes. 玻璃对链球菌的吸附:大分子溶质的影响。

Acta pathologica et microbiologica Scandinavica. Section B, Microbiology Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1977.TB01673.X

D. Ørstavik

引用次数: 0