{"title":"呼吸道合胞病毒感染的免疫荧光快速诊断:鼻咽吸吸标本制备的简化程序。","authors":"Gabriel Ånestad, Osvald Ragnvald Mœhle","doi":"10.1111/J.1699-0463.1981.TB00190_89B.X","DOIUrl":null,"url":null,"abstract":"A preliminary report on a simplified procedure for the preparation of nasopharyngeal suction specimens making direct smears of aspirated material is presented. The smears were examined by the indirect immunofluorescence (IF) technique for the presence of respiratory syncytial (RS) virus antigens. Specimens form 99 children with acute respiratory tract illnesses were collected during the winter season 1978-79, and RS virus was identified in samples from 45 of these patients. Serological investigations run in parallel showed fairly good correlation with the IF examinations of the smears. This simplified procedure for the preparation of nasopharyngeal suction specimens may be recommended when the conventional preparation cannot readily be performed.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"55 1","pages":"285-287"},"PeriodicalIF":0.0000,"publicationDate":"2009-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens.\",\"authors\":\"Gabriel Ånestad, Osvald Ragnvald Mœhle\",\"doi\":\"10.1111/J.1699-0463.1981.TB00190_89B.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A preliminary report on a simplified procedure for the preparation of nasopharyngeal suction specimens making direct smears of aspirated material is presented. The smears were examined by the indirect immunofluorescence (IF) technique for the presence of respiratory syncytial (RS) virus antigens. Specimens form 99 children with acute respiratory tract illnesses were collected during the winter season 1978-79, and RS virus was identified in samples from 45 of these patients. Serological investigations run in parallel showed fairly good correlation with the IF examinations of the smears. This simplified procedure for the preparation of nasopharyngeal suction specimens may be recommended when the conventional preparation cannot readily be performed.\",\"PeriodicalId\":75410,\"journal\":{\"name\":\"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology\",\"volume\":\"55 1\",\"pages\":\"285-287\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-08-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/J.1699-0463.1981.TB00190_89B.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1699-0463.1981.TB00190_89B.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Rapid diagnosis of respiratory syncytial (RS) virus infection by immunofluorescence: a simplified procedure for the preparation of nasopharyngeal suction specimens.
A preliminary report on a simplified procedure for the preparation of nasopharyngeal suction specimens making direct smears of aspirated material is presented. The smears were examined by the indirect immunofluorescence (IF) technique for the presence of respiratory syncytial (RS) virus antigens. Specimens form 99 children with acute respiratory tract illnesses were collected during the winter season 1978-79, and RS virus was identified in samples from 45 of these patients. Serological investigations run in parallel showed fairly good correlation with the IF examinations of the smears. This simplified procedure for the preparation of nasopharyngeal suction specimens may be recommended when the conventional preparation cannot readily be performed.
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