{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. I. Comparison of thirteen O groups of Ps. aeruginosa, with a polyvalent Ps. aeruginosa antigen-antibody reference system.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1975.TB00108.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1975.TB00108.X","url":null,"abstract":"Serologic cross-reactions between 26 strains of Pseudomonas aeruginosa representing 13 O groups were studied by various quantitative immunoelectrophoretic techniques. As reference system was used a polyvalent Ps. aeruginosa antigen and a corresponding rabbit antiserum. Fifty-one (93 per cent) of the 55 Ps. aeruginosa antigens in the reference system were present in all the strains and corresponding antibodies in the reference system could be completely absorbed by all the strains. Complete cross-reactivity was also found between antigens of the reference system and 3 of the 4 antigens present only in some of the strains. The last of the 4 antigens not present in all the strains could only absorb part of the corresponding antibodies in the reference system. Absorption experiments with whole heat-killed bacteria indicate that this antigen is related to the O group antigens of Ps. aeruginosa. None of the antigens of the reference system were related to the mucoid substance produced by some strains of this bacterium.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"6 1","pages":"321-7"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81819290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EXPERIMENTAL TOXOPLASMOSIS IN MICE AND RABBITS","authors":"E. Pettersen","doi":"10.1111/J.1699-0463.1977.TB01679.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1977.TB01679.X","url":null,"abstract":"Avirulent Toxoplasma gondii were made virulent by passages in mice, and then attenuated by storage. In contrast to the avirulent parasites, attenuated organisms did not appear to form cysts in mice and rabbits, although parasites could be isolated from such animals as long as three months after their inoculation. Rabbits infected with parasites of attenuated - virulence had high antibody levels, and survived challenge with virulent Toxoplasma gondii. Cysts were not detected in the brains of such rabbits following this second infection. By following the generation of Toxoplasma gondii in mouse peritoneal cavities, it was found that attenuated parasites resembled avirulent more than virulent organisms. Although the effect of attenuation was mainly on the parental generation of parasites, some genetic effect seemed to be involved as well.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"466 1","pages":"95-102"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79883138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Radiorespirometric studies in genus Neisseria. 2. The catabolism of glutamate and fumarate.","authors":"E. Holten","doi":"10.1111/j.1699-0463.1976.tb01893.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1976.tb01893.x","url":null,"abstract":"The catabolism of glutamate and fumarate was studied by radiorespirometry in selected Neisseria species. The tricarboxylic acid cycle is functioning in all species tested, in spite of the known absence of in vitro malate dehydrogenase activity in N. meningitidis, N. gonorrhoeae and N. cinerea. The results imply a pyridine nucleotide independent oxidation of malate. The oxidation of glutamate is less complete in the presence of phosphate. In N. meningitidis, N. perflava, N. flava, N. subflava and N. lactamica the catabolism of fumarate was slow and incomplete in the absence of glutamate.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"15 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77545621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NDV‐O AGGLUTININS IN MYCOPLASMA PNEUMONIAE INFECTIONS: ASSOCIATION WITH PNEUMONIA","authors":"R. Pyhälä","doi":"10.1111/J.1699-0463.1976.TB01932.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01932.X","url":null,"abstract":"Agglutinins against human group O erythrocytes modified by the B1 strain of Newcastle disease virus were studied in paired sera of 148 patients with a 4-fold or greater rise in complement-fixing M. pneumoniae antibodies. The proportion of cases with a significant rise in NDV-O agglutinins was higher among the patients with pneumonia than among those with neurological or other clinical manifestations.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"37 1","pages":"240-244"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78945108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"AGAROSE ELECTROPHORESIS COMBINED WITH SECOND DIMENSIONAL CETAVLON PRECIPITATION. A NEW METHOD FOR DEMONSTRATION OF ACIDIC POLYSACCHARIDE K ANTIGENS","authors":"F. Ørskov","doi":"10.1111/J.1699-0463.1976.TB01946.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01946.X","url":null,"abstract":"A new method was developed for detection of acidic polysaccharides in bacterial extracts of E. coli using agarose electrophoresis of the extracts combined with Cetavlon precipitation in the second dimension.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"44 1","pages":"319-320"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76441238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biological conditions influencing the focal necrotic hepatitis test for differentiation between herpes simplex virus types 1 and 2.","authors":"S. C. Mogenseny","doi":"10.1111/J.1699-0463.1976.TB01918.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01918.X","url":null,"abstract":"Some biological conditions of the focal necrotic hepatitis test for the differentiation between herpes simplex virus (HSV) types 1 and 2 were investigated. Most of 13 different strains of mice tested were found usable in the test. An upper age limit (4 weeks) for the appearance of focal necrotic liver lesions was found in one strain of mice, while this was not seen in another strain. The minimum dose in 3- to 4-week-old mice was found to be as small as 10(2) to 10(3) p.f.u. in 0.1 ml of diluent. Suckling rats and hamsters, aged up to 7 and 14 days, respectively, were found to be convenient as alternative test animals. Finally, it was observed that focal necrotic hepatitis did not develop in the nude mouse with thymic aplasia on intraperitoneal inoculation of HSV type 2. The possible involvement of the thymus in the pathogenesis of the focal necrotic lesions is briefly discussed.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"33 1","pages":"154-158"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87230692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sorption of Streptococcus faecium to glass.","authors":"D. Ørstavik","doi":"10.1111/J.1699-0463.1977.TB01672.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1977.TB01672.X","url":null,"abstract":"A method has been developed by which to study the sorption of Streptococcus faecium to soda-lime cover glasses. Conditions were chosen to minimize the influence on sorption of bacterial polymer production, passive sorption being studied rather than attachment mediated by metabolic activities. Sorption of S. faecium increased with increasing temperature (to 50 degrees C), time, and cell concentration, but equilibrium apparently was not reached even after incubation for 8 hours or at a cell concentration of 3 X 10(10) per ml. Sorption increased with solute molarity up to 0.1 M concentration of NaCl and KCl, indicating an effect of the electrical double layers on the apposition of cells to the glass surface. Desorption of bacteria could be obtained after multiple washings of the glasses in buffer or by the action of Tween 80, but not if sorbed bacteria were left in distilled water, various salt solutions, urea, or in suspensions of unlabelled bacteria. It was concluded that sorption occurred as a result of chemical interactions between the glass and the cell surface. Tween 80 at a concentration of 1 per cent inhibited sorption to 26 per cent of buffer controls, 2 M urea was less effective, and 1 M NaCl was without effect. It is suggested that hydrophobic interactions may be of importance in the binding of S. faecium to glass.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"38 1","pages":"38-46"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88805488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"OCCURRENCE OF REO‐LIKE VIRUSES IN YOUNG CHILDREN WITH ACUTE GASTROENTERITIS","authors":"B. Tufvesson, T. Johnsson","doi":"10.1111/J.1699-0463.1976.TB01896.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1976.TB01896.X","url":null,"abstract":"In the course of a six-month-study of acute gastroenteritis in children of ages up to six years, a reo-like virus was found in 54 per cent of the faecal specimens obtained at an early stage of the disease, using electron microscopy as screening test. By means of a concentrated complement fixation antigen, composed of a related calf diarrhoea virus cultivated in tissue culture, the rise in titre was found to be significant in 96 per cent of the patients whose faeces contained the reo-like virus. Antibodies were present in the remaining 4 per cent without rise in titre. In 10 per cent of the cases with gastroenteritis infection was caused by adenovirus or Salmonella. A probable aetiological agent was found in 71 per cent of the patients. It applies to 33 per cent of all cases caused by the reo-like virus that they were nosocomial infections.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"55 1","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81901429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Purine metabolism in Neisseria meningitidis. 3. Utilization of exogenous hypoxanthine, guanine and xanthine.","authors":"S. Jyssum","doi":"10.1111/j.1699-0463.1975.tb00118.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1975.tb00118.x","url":null,"abstract":"Whole cells of Neisseria meningitidis have been found to utilize exogenous radioactive hypoxanthine, guanine and xanthine. When hypoxanthine was the precursor, the pools of both the adenine and the guanine 5'-ribonucleotides were labelled. Guanine and xanthine were utilized with labelling of the pool of the guanine 5'-ribonucleotides only. Crude extracts from N. meningitidis were found to have activities corresponding to hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and another phosphoribosyltransferase which seems to exhibit specificity for guanine and xanthine. Crude extracts phosphorylated guanosine 5'-monophosphate to guanosine 5'-triphosphate in the presence of adenosine 5'-triphosphate (ATP) and MgCl2.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"55 1 1","pages":"397-406"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82690349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The serology of Pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. III. Reproducibility of a polyvalent P. aeruginosa reference standard-antigen.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1975.TB00122.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1975.TB00122.X","url":null,"abstract":"The reproducibility of a polyvalent Pseudomonas aeruginosa antigen (St-Ag) composed of a mixture of antigens from 4 O groups of this bacterium has been studied. Ten batches of St-Ag were produced, and each of these and each of the 10 batches of antigens from the 4 strains of P. aeruginosa were compared with St-Ag batch 1 by means of quantitative immunoelectrophoretic methods and a polyvalent antiserum (St-Ab) raised against St-Ag. Fifty-three of the 55 antigens of St-Ag were stable and could be reproduced with reasonable precision in all 10 batches, and 3 of the 4 strains of P. aeruginosa were stable in antigen composition in all batches. One of the strains (0-5A) had lost 2 antigens in the last 5 batches, and the concentrations of 7 other antigens were simultaneously changed, reflecting a smooth-rough dissociation. The disappearance of the 2 antigens in the latest 5 batches of 0-5A was also reflected in similar changes in the antigen composition of the latest 5 batches of St-Ag.","PeriodicalId":75410,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B, Microbiology","volume":"1 1","pages":"433-42"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82713819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}