Diana-Bianca Zalischi, Laura Cătană, Laura-Cristina Ştefănuţ, Andra Popescu, Ilinca Iozon, Andreea Muntean, Mihai Cernea
{"title":"Overdose Effects of Medetomidine and Fentanyl in Rats: Reflex, Vital and Analgesic Parameters as Predictive Markers.","authors":"Diana-Bianca Zalischi, Laura Cătană, Laura-Cristina Ştefănuţ, Andra Popescu, Ilinca Iozon, Andreea Muntean, Mihai Cernea","doi":"10.2147/VMRR.S572918","DOIUrl":"10.2147/VMRR.S572918","url":null,"abstract":"<p><strong>Objective: </strong>To determine the overdose (OD) of medetomidine (MT) in Wistar rats and to evaluate the potentiating effect of fentanyl (FT) when co-administered. The objective was to identify the dose inducing complete abolition of five standard reflexes and significant changes of: heart rate (HR), respiratory rate (RR), tissular oxygen saturation (SpO<sub>2</sub>), rectal temperature (RT).</p><p><strong>Methods: </strong>Eight groups of Wistar rats (2 males and 2 females each) were used; the group showing the strongest effect was supplemented with 2 additional males and females. In phase one, four groups received intramuscular MT at 0.01, 0.03, 0.05, or 0.1 mg/kg. In phase two, the effective MT dose (0.1 mg/kg) was combined with FT at 0.025, 0.05, 0.075, or 0.1 mg/kg to assess synergistic effects. Reflexes, antinociception (via cold ethanol tail-flick test), and vital signs were recorded at baseline, during OD induction, and up to 60 minutes post-administration. The animals were not euthanised and allowed to recover.</p><p><strong>Results: </strong>The 0.1 mg/kg MT dose produced the shortest time to OD, while for the combination of 0.1 mg/kg MT with 0.1 mg/kg FT, OD time was 48% faster. Fentanyl significantly attenuated the bradycardia induced by MT (p < 0.0001), while RR depression was less pronounced at OD, but became evident at Pt60. The males from MT combined with FT group showed a shorter induction time of OD compared to males from MT alone group. In females, the effect was statistically robust (p = 0.0064), showing a significantly shorter induction time.</p><p><strong>Conclusion: </strong>The MT (0.1 mg/kg) and FT (0.1 mg/kg) combination produced the most consistent and pronounced OD, characterized by rapid reflex loss, marked physiological suppression, and delayed recovery. This interaction carries significant translational relevance for both veterinary anesthesia and human medicine in the context of the opioid crisis.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"17 ","pages":"572918"},"PeriodicalIF":2.5,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12960050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147380043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lulu Xie, Yimin Zhou, Xinyu Zhang, Tian Lan, Wenchao Sun
{"title":"Global Trends in MPOX Research (2014-2025): A Bibliometric Analysis and Overview.","authors":"Lulu Xie, Yimin Zhou, Xinyu Zhang, Tian Lan, Wenchao Sun","doi":"10.2147/VMRR.S564307","DOIUrl":"10.2147/VMRR.S564307","url":null,"abstract":"<p><p>The 2022 global monkeypox outbreak fuelled a surge in related research. In this study, a bibliometric analysis of 2,076 monkeypox-related articles indexed in the Web of Science Core Collection (from January 2014 to June 2025) was performed, and VOSviewer was used to visualize keyword co-occurrence networks and collaboration patterns among countries and institutions. Unlike existing studies, the latest data for 2024-2025 are incorporated; postoutbreak research trends, collaborative patterns, and PHEIC-driven resource aggregation effects are systematically incorporated; and gaps in dynamic and multifaceted analyses of the field are addressed. The results revealed a three-stage global research output: low accumulation (2014-2021), a sharp surge after 2022, and sustained high levels after a peak in 2023. The core research clusters focused on viral transmission dynamics and clinical interventions. Country contributions showed a pyramidal hierarchy, with the US leading, followed by China, India, and Saudi Arabia; government health agencies and academic institutions codominated collaborations. The findings confirm the role of the PHEIC in concentrating scientific resources, emphasizing the importance of strengthening international collaboration to address future epidemic challenges.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"99-112"},"PeriodicalIF":2.5,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12719622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145822345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diversity of Multi-Drug Resistance Genes in <i>Escherichia coli</i> Isolated from Poultry in Southern Togo.","authors":"Malibida Dolou, Essokedi Tchedie, Essolakina Dolou, Akoele Siliadin, Amivi M Godonou, Abla Ahouefa Konou, Essodina Talaki, Simplice D Karou, Mounerou Salou, Anoumou Yaotse Dagnra","doi":"10.2147/VMRR.S544186","DOIUrl":"10.2147/VMRR.S544186","url":null,"abstract":"<p><strong>Background and purpose: </strong>The medical sector is facing therapeutic dead ends due to antimicrobial resistance (AMR), which leads to human death and economic losses for poultry farmers. This study aimed to investigate the diversity of resistance genes in Enterobacteriaceae on poultry farms.</p><p><strong>Methods: </strong>Illumina sequencing was used for the cultivation of 184 poultry fecal samples, identification using the API 10S Biomerieux gallery, antibiotic susceptibility testing by disk diffusion, and DNA extraction using the Biocentric Bruker automated system.</p><p><strong>Results: </strong>Among the 164 isolates, <i>Escherichia coli</i> was the most isolated species (84.78%, n = 156), followed by <i>Klebsiella pneumoniae</i> (3.26%, n = 06), and <i>Salmonella</i> spp. (0.01%, n = 02). Among 156 <i>Escherichia coli</i> strains, 10% (n = 18) were extended-spectrum bêta-lactamase (ESBL) producers. Among the 13 sequenced ESBL-producing <i>E. coli</i> strains, 53 resistance genes were identified with moderate variability and some were strain-specific. Co-localization of plasmids with virulence genes was also observed. Several <i>blaTEM</i> variants that encode beta-lactam resistance have been frequently detected. The <i>ompK37</i> and <i>ompK36</i> mutants coding for carbapenemase production were identified in one strain. One strain carried <i>mcr-1.1</i> gene.</p><p><strong>Conclusion: </strong>Antibiotics used in poultry farming contribute to the selection of resistant and highly virulent clones. Strengthening regulations on antibiotic use in poultry farming is necessary.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"77-98"},"PeriodicalIF":2.5,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12669059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145672560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serum Onconeural Antibodies in Dogs and Cats for Early Diagnosis of Cancer.","authors":"Marianna Agassandian, Khristofor Agassandian","doi":"10.2147/VMRR.S537744","DOIUrl":"10.2147/VMRR.S537744","url":null,"abstract":"<p><p>Cancer is one of the leading causes of death in dogs and cats worldwide, and effective early detection techniques and reliable therapies are still lacking. Given the high demand for early cancer detection and differentiation in veterinary diagnostics, we developed and validated a new diagnostic approach to assess onconeural antibodies, also known as high-risk antibodies, in dog and cat blood serum samples. We determined the presence of systemic onconeural/high-risk (ONHR) antibodies, their suitability for early cancer diagnostics, and the feasibility of differentiating various malignancies. Our results identified several ONHR antibodies in 0.1 mL of specimens by the immunoblot-based technique, which was confirmed by indirect immunofluorescence assay. The diagnostic performance for detecting identified antibodies has demonstrated >95% sensitivity in dogs, >93% sensitivity in cats, as well as >97% specificity in dogs, and >95% specificity in cats. Thus, our data provide the first proof-of-principle that onconeural antibodies can be detected in dogs and cats, and their identification in serum might serve as a new tool for early cancer diagnosis.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"49-75"},"PeriodicalIF":2.5,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12671446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145672577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a Novel Loop-Mediated Isothermal Amplification (LAMP) for Rapid Diagnosis of Newcastle Disease in Field and Resource-Limited Areas.","authors":"Berihun Dires Mihiretu, Tatsufumi Usui, Tesfaye Rufael Chibssa, Abde Aliy Mohammed, Tsuyoshi Yamaguchi","doi":"10.2147/VMRR.S549952","DOIUrl":"10.2147/VMRR.S549952","url":null,"abstract":"<p><strong>Background: </strong>Newcastle Disease (ND) is a highly contagious and fatal poultry disease caused by Newcastle disease virus (NDV) and has a global distribution. To control this disease, a rapid method for diagnosing it is needed. PCR-based molecular diagnostics such as real-time reverse transcription-PCR (RRT-PCR) for detecting NDV genes have been conducted worldwide. However, because PCR methods are time-consuming and require a well-equipped laboratory, they are not well-suited for use in field and resource limited areas.</p><p><strong>Methods: </strong>In this study, we established a field-friendly loop-mediated isothermal amplification (LAMP) assay for NDV diagnosis using primers targeting the highly conserved L gene, which bypasses the need for RNA extraction and makes it more suitable for field application than previously reported LAMP methods.</p><p><strong>Results: </strong>Our LAMP method is capable of detecting a broad range of NDV genotypes and showed no cross-reactivity with other avian viral diseases or the host genome. The reaction is completed within 35 minutes of incubation at 65°C. NDV was successfully detected directly from swab and tissue samples without the need for conventional RNA extraction.</p><p><strong>Conclusion: </strong>The LAMP method developed in this study offers a rapid, affordable, and field-friendly diagnostic tool for NDV detection.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"39-47"},"PeriodicalIF":2.5,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12597256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145491034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Martin J Oettler, Gerald Stumpf, Katja Schulz, Matthias Todte, Klim Hüttner, Heidemarie Heyne, Thomas C Mettenleiter, Franz J Conraths, Carola Sauter-Louis
{"title":"Strict Biosecurity and Epidemiological Segmentation Enable Partial Culling During a Highly Pathogenic Avian Influenza Outbreak.","authors":"Martin J Oettler, Gerald Stumpf, Katja Schulz, Matthias Todte, Klim Hüttner, Heidemarie Heyne, Thomas C Mettenleiter, Franz J Conraths, Carola Sauter-Louis","doi":"10.2147/VMRR.S539444","DOIUrl":"10.2147/VMRR.S539444","url":null,"abstract":"<p><strong>Background: </strong>The mandatory procedures to be followed after official confirmation of an outbreak of category A animal infectious diseases, including highly pathogenic avian influenza (HPAI), is laid down in European and national legislation. Typically, an outbreak of HPAI results in the destruction of the entire poultry population on the affected holding.</p><p><strong>Case presentation: </strong>The presented case reports a deviation from this approach, demonstrating the practicality of partial culling in a highly biosecure, epidemiologically segmented holding. These on-site circumstances together with the specific risk assessment led to the elimination of only the affected unit, thereby inhibiting the further spread of the disease. After the destruction of the respective unit (farm), the other farms were closely monitored and tested continuously negative for HPAI virus (HPAIV) despite intensive systematic sampling. In the end, this procedure saved approximately 138,000 animals, ie 75% of the poultry population of the holding from destruction.</p><p><strong>Conclusion: </strong>This case demonstrates the effectiveness of proper management and high-level biosecurity to avoid excessive destruction of animals in case of an infectious disease outbreak. It might be suitable as a best-practice example in similar situations.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"25-37"},"PeriodicalIF":2.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Owen Hurst, Alicia Mastrocco, Jennifer Prittie, Ashley Hadala, Joel Green Weltman
{"title":"Retrospective Evaluation of the Severity of Creatine Kinase Elevation in Canine and Feline Trauma Patients as a Predictor of Morbidity and Mortality.","authors":"Owen Hurst, Alicia Mastrocco, Jennifer Prittie, Ashley Hadala, Joel Green Weltman","doi":"10.2147/VMRR.S517141","DOIUrl":"10.2147/VMRR.S517141","url":null,"abstract":"<p><strong>Purpose: </strong>To assess if severity of creatine kinase (CK) elevation in veterinary trauma patients is associated with overall patient morbidity (need for blood products or surgery, prolonged hospitalization) and mortality.</p><p><strong>Patients and methods: </strong>Five hundred and eight-three dogs and cats experiencing trauma with an admission CK > 1000 U/L over a 15-year period were included in this study. The population was further stratified based on severity of CK elevation to include 161 dogs and 133 cats with admission CK > 5000 U/L, and 211 dogs and 78 cats with admission CK between 1000 and 5000 U/L. These groups were then compared for likelihood of trauma-associated morbidity, including increased need for blood products, surgical intervention, and/or hospitalization time. The likelihood of mortality was also compared between groups.</p><p><strong>Results: </strong>The need for packed red blood cell transfusion and length of hospitalization were significantly increased in traumatized dogs and cats with CK > 5000 U/L. Higher CK was not associated with increased surgical needs. Dogs with CK > 5000 U/L had significantly higher mortality rate compared to <5000 U/L.</p><p><strong>Conclusion: </strong>A higher degree of CK elevation is associated with need for blood products and prolonged hospitalization in dogs and cats and higher mortality in dogs. Evaluation of the severity of CK levels on presentation, and serial evaluation of the same, may aid in the assessment of trauma severity and prognosis in veterinary trauma patients.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"17-23"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Peripheral Eosinophilia in a Confirmed Case of Canine Acute Eosinophilic Dermatitis with Edema (Wells-Like Syndrome).","authors":"Ashley Pace, Jeanette Hendricks","doi":"10.2147/VMRR.S517686","DOIUrl":"10.2147/VMRR.S517686","url":null,"abstract":"<p><p>This case report describes a peripheral eosinophilia in a dog diagnosed with canine acute eosinophilic dermatitis with edema (CAEDE). A 1-year-old female spayed Terrier Mix canine presented as a referral from their primary care veterinarian for gastrointestinal signs that were recently treated, but ongoing, and a new dermatopathy. Her leukogram revealed an eosinophilia, not previously present at the onset of her gastrointestinal signs, and skin biopsies were consistent with diagnosis of CAEDE. Although the definitive cause of the development of CAEDE in this patient is ultimately unknown, the patient made a full recovery following treatment with corticosteroids over a three-week treatment course. To the authors' knowledge, this is the first report of a confirmed diagnosis of CAEDE to present with peripheral eosinophilia on complete blood count (IDEXX Procyte Dx).</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"9-16"},"PeriodicalIF":1.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana A Jiménez-Alonso, Carmen G Pérez-Santana, Sara E Cazorla-Rivero, Francisco Rodríguez-Esparragón, Enrique Rodríguez Grau-Bassas
{"title":"Clinical Staging and Histopathological Grading of Inguinal Squamous Cell Carcinoma in Dogs: A Case Series Study.","authors":"Ana A Jiménez-Alonso, Carmen G Pérez-Santana, Sara E Cazorla-Rivero, Francisco Rodríguez-Esparragón, Enrique Rodríguez Grau-Bassas","doi":"10.2147/VMRR.S500469","DOIUrl":"10.2147/VMRR.S500469","url":null,"abstract":"<p><strong>Introduction: </strong>Canine cutaneous squamous cell carcinoma (SCC) is a locally invasive tumor with a variable prognosis.</p><p><strong>Materials and methods: </strong>This study evaluated clinical stage and histopathological grade as prognostic factors. Eleven dogs with inguinal SCC underwent surgery. All dogs presented varying degrees of atopic skin owing to prolonged outdoor ultraviolet (UV) exposure. Tumors are located primarily in the ventro inguinal area, affecting the prepuce and scrotum in males. Clinical staging (TNM system) was performed through abdominal ultrasound and palpation of regional lymph nodes, and histopathologically, Broder grading was applied.</p><p><strong>Discussion: </strong>The survival time (ST) and disease-free interval (DFI) were analyzed, and the median ST was 738 days for Grade 1 tumors compared with 135 days for Grade 4 tumors. Staging correlated with metastatic risk (1/11 patients).</p><p><strong>Conclusion: </strong>This study highlights the importance of histological grade as a prognostic factor for canine cutaneous SCC and surgery as a treatment of choice and emphasizes the need for further studies on disease progression and treatment outcomes in veterinary medicine.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ester Taberner, Marta Gibert, Carlos Montbrau, Irene Muñoz Ruiz, Joaquim Mallorquí, Héctor Santo Tomás, Antoni Prenafeta, Ricard March
{"title":"Efficacy of Vaccination with the DIVENCE<sup>®</sup> Vaccine Against Bovine Viral Diarrhea Virus Types 1 and 2 in Terms of Fetal Protection.","authors":"Ester Taberner, Marta Gibert, Carlos Montbrau, Irene Muñoz Ruiz, Joaquim Mallorquí, Héctor Santo Tomás, Antoni Prenafeta, Ricard March","doi":"10.2147/VMRR.S474655","DOIUrl":"10.2147/VMRR.S474655","url":null,"abstract":"<p><strong>Purpose: </strong>To demonstrate the efficacy of DIVENCE<sup>®</sup>, a vaccine against BVDV types 1 and 2 (BVDV-1 and BVDV-2) transplacental infection, following a booster regimen in heifers.</p><p><strong>Materials and methods: </strong>Calves of two-to-three months of age were given two intramuscular doses three weeks apart and a booster vaccine six months later. Efficacy was evaluated by means of a challenge with virulent BVDV-1 or BVDV-2 administered via the intranasal route at 85 days of gestation. Clinical signs, serology, viral shedding, WBC count and viremia were monitored after the challenge. Sixty-six days post-challenge, the fetuses were assessed for BVDV to detect transplacental infection.</p><p><strong>Results: </strong>Vaccinated animals showed a significant (<i>p</i> < 0.05) reduction in hyperthermia after both challenges. The WBC counts in vaccinated animals were significantly (<i>p</i> < 0.05) higher than in control animals on Days 5 and 6 after both challenges. Vaccinated animals exhibited no shedding after BVDV-1 challenge and the percentage of shedding animals was significantly (<i>p</i> < 0.05) higher among control animals compared to vaccinated animals after BVDV-2 challenge. Viremia were detected in pregnant heifers from all control animals, while only 3/14 and 3/17 pregnant vaccinated heifers showed viremia after BVDV-1 and BVDV-2 challenges, respectively. All the fetuses (n=8) from the control animals were positive for BVDV-1 via virus titration after BVDV-1 challenge. Only one out of fourteen fetuses from the vaccinated animals was positive for BVDV-1. After BVDV-2 challenge, all the control animals had BVDV-2 in all fetal tissues assessed and only one of the seventeen vaccinated animals had BVDV-2 in its fetal samples.</p><p><strong>Conclusion: </strong>DIVENCE<sup>®</sup> administered prior to breeding protected 94% of the fetuses against BVDV transplacental infection overall across both challenge trials (BVDV-1 and BVDV-2). A reduction in the hyperthermia, leukopenia, viral shedding, and viremia in vaccinated animals post-challenge with BVDV-1 and BVDV-2 was achieved. The efficacy of DIVENCE<sup>®</sup> against BVDV-1 and BVDV-2 transplacental infection has been demonstrated in this study.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"221-238"},"PeriodicalIF":1.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}