Veterinary medicine (Auckland, N.Z.)最新文献

{"title":"Strict Biosecurity and Epidemiological Segmentation Enable Partial Culling During a Highly Pathogenic Avian Influenza Outbreak.","authors":"Martin J Oettler, Gerald Stumpf, Katja Schulz, Matthias Todte, Klim Hüttner, Heidemarie Heyne, Thomas C Mettenleiter, Franz J Conraths, Carola Sauter-Louis","doi":"10.2147/VMRR.S539444","DOIUrl":"10.2147/VMRR.S539444","url":null,"abstract":"<p><strong>Background: </strong>The mandatory procedures to be followed after official confirmation of an outbreak of category A animal infectious diseases, including highly pathogenic avian influenza (HPAI), is laid down in European and national legislation. Typically, an outbreak of HPAI results in the destruction of the entire poultry population on the affected holding.</p><p><strong>Case presentation: </strong>The presented case reports a deviation from this approach, demonstrating the practicality of partial culling in a highly biosecure, epidemiologically segmented holding. These on-site circumstances together with the specific risk assessment led to the elimination of only the affected unit, thereby inhibiting the further spread of the disease. After the destruction of the respective unit (farm), the other farms were closely monitored and tested continuously negative for HPAI virus (HPAIV) despite intensive systematic sampling. In the end, this procedure saved approximately 138,000 animals, ie 75% of the poultry population of the holding from destruction.</p><p><strong>Conclusion: </strong>This case demonstrates the effectiveness of proper management and high-level biosecurity to avoid excessive destruction of animals in case of an infectious disease outbreak. It might be suitable as a best-practice example in similar situations.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"25-37"},"PeriodicalIF":2.5,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Evaluation of the Severity of Creatine Kinase Elevation in Canine and Feline Trauma Patients as a Predictor of Morbidity and Mortality.","authors":"Owen Hurst, Alicia Mastrocco, Jennifer Prittie, Ashley Hadala, Joel Green Weltman","doi":"10.2147/VMRR.S517141","DOIUrl":"10.2147/VMRR.S517141","url":null,"abstract":"<p><strong>Purpose: </strong>To assess if severity of creatine kinase (CK) elevation in veterinary trauma patients is associated with overall patient morbidity (need for blood products or surgery, prolonged hospitalization) and mortality.</p><p><strong>Patients and methods: </strong>Five hundred and eight-three dogs and cats experiencing trauma with an admission CK > 1000 U/L over a 15-year period were included in this study. The population was further stratified based on severity of CK elevation to include 161 dogs and 133 cats with admission CK > 5000 U/L, and 211 dogs and 78 cats with admission CK between 1000 and 5000 U/L. These groups were then compared for likelihood of trauma-associated morbidity, including increased need for blood products, surgical intervention, and/or hospitalization time. The likelihood of mortality was also compared between groups.</p><p><strong>Results: </strong>The need for packed red blood cell transfusion and length of hospitalization were significantly increased in traumatized dogs and cats with CK > 5000 U/L. Higher CK was not associated with increased surgical needs. Dogs with CK > 5000 U/L had significantly higher mortality rate compared to <5000 U/L.</p><p><strong>Conclusion: </strong>A higher degree of CK elevation is associated with need for blood products and prolonged hospitalization in dogs and cats and higher mortality in dogs. Evaluation of the severity of CK levels on presentation, and serial evaluation of the same, may aid in the assessment of trauma severity and prognosis in veterinary trauma patients.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"17-23"},"PeriodicalIF":2.5,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12323786/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144791027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Eosinophilia in a Confirmed Case of Canine Acute Eosinophilic Dermatitis with Edema (Wells-Like Syndrome).","authors":"Ashley Pace, Jeanette Hendricks","doi":"10.2147/VMRR.S517686","DOIUrl":"10.2147/VMRR.S517686","url":null,"abstract":"<p><p>This case report describes a peripheral eosinophilia in a dog diagnosed with canine acute eosinophilic dermatitis with edema (CAEDE). A 1-year-old female spayed Terrier Mix canine presented as a referral from their primary care veterinarian for gastrointestinal signs that were recently treated, but ongoing, and a new dermatopathy. Her leukogram revealed an eosinophilia, not previously present at the onset of her gastrointestinal signs, and skin biopsies were consistent with diagnosis of CAEDE. Although the definitive cause of the development of CAEDE in this patient is ultimately unknown, the patient made a full recovery following treatment with corticosteroids over a three-week treatment course. To the authors' knowledge, this is the first report of a confirmed diagnosis of CAEDE to present with peripheral eosinophilia on complete blood count (IDEXX Procyte Dx).</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"9-16"},"PeriodicalIF":1.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227095/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Staging and Histopathological Grading of Inguinal Squamous Cell Carcinoma in Dogs: A Case Series Study.","authors":"Ana A Jiménez-Alonso, Carmen G Pérez-Santana, Sara E Cazorla-Rivero, Francisco Rodríguez-Esparragón, Enrique Rodríguez Grau-Bassas","doi":"10.2147/VMRR.S500469","DOIUrl":"10.2147/VMRR.S500469","url":null,"abstract":"<p><strong>Introduction: </strong>Canine cutaneous squamous cell carcinoma (SCC) is a locally invasive tumor with a variable prognosis.</p><p><strong>Materials and methods: </strong>This study evaluated clinical stage and histopathological grade as prognostic factors. Eleven dogs with inguinal SCC underwent surgery. All dogs presented varying degrees of atopic skin owing to prolonged outdoor ultraviolet (UV) exposure. Tumors are located primarily in the ventro inguinal area, affecting the prepuce and scrotum in males. Clinical staging (TNM system) was performed through abdominal ultrasound and palpation of regional lymph nodes, and histopathologically, Broder grading was applied.</p><p><strong>Discussion: </strong>The survival time (ST) and disease-free interval (DFI) were analyzed, and the median ST was 738 days for Grade 1 tumors compared with 135 days for Grade 4 tumors. Staging correlated with metastatic risk (1/11 patients).</p><p><strong>Conclusion: </strong>This study highlights the importance of histological grade as a prognostic factor for canine cutaneous SCC and surgery as a treatment of choice and emphasizes the need for further studies on disease progression and treatment outcomes in veterinary medicine.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"16 ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12127521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Vaccination with the DIVENCE^® Vaccine Against Bovine Viral Diarrhea Virus Types 1 and 2 in Terms of Fetal Protection.","authors":"Ester Taberner, Marta Gibert, Carlos Montbrau, Irene Muñoz Ruiz, Joaquim Mallorquí, Héctor Santo Tomás, Antoni Prenafeta, Ricard March","doi":"10.2147/VMRR.S474655","DOIUrl":"10.2147/VMRR.S474655","url":null,"abstract":"<p><strong>Purpose: </strong>To demonstrate the efficacy of DIVENCE^®, a vaccine against BVDV types 1 and 2 (BVDV-1 and BVDV-2) transplacental infection, following a booster regimen in heifers.</p><p><strong>Materials and methods: </strong>Calves of two-to-three months of age were given two intramuscular doses three weeks apart and a booster vaccine six months later. Efficacy was evaluated by means of a challenge with virulent BVDV-1 or BVDV-2 administered via the intranasal route at 85 days of gestation. Clinical signs, serology, viral shedding, WBC count and viremia were monitored after the challenge. Sixty-six days post-challenge, the fetuses were assessed for BVDV to detect transplacental infection.</p><p><strong>Results: </strong>Vaccinated animals showed a significant (<i>p</i> < 0.05) reduction in hyperthermia after both challenges. The WBC counts in vaccinated animals were significantly (<i>p</i> < 0.05) higher than in control animals on Days 5 and 6 after both challenges. Vaccinated animals exhibited no shedding after BVDV-1 challenge and the percentage of shedding animals was significantly (<i>p</i> < 0.05) higher among control animals compared to vaccinated animals after BVDV-2 challenge. Viremia were detected in pregnant heifers from all control animals, while only 3/14 and 3/17 pregnant vaccinated heifers showed viremia after BVDV-1 and BVDV-2 challenges, respectively. All the fetuses (n=8) from the control animals were positive for BVDV-1 via virus titration after BVDV-1 challenge. Only one out of fourteen fetuses from the vaccinated animals was positive for BVDV-1. After BVDV-2 challenge, all the control animals had BVDV-2 in all fetal tissues assessed and only one of the seventeen vaccinated animals had BVDV-2 in its fetal samples.</p><p><strong>Conclusion: </strong>DIVENCE^® administered prior to breeding protected 94% of the fetuses against BVDV transplacental infection overall across both challenge trials (BVDV-1 and BVDV-2). A reduction in the hyperthermia, leukopenia, viral shedding, and viremia in vaccinated animals post-challenge with BVDV-1 and BVDV-2 was achieved. The efficacy of DIVENCE^® against BVDV-1 and BVDV-2 transplacental infection has been demonstrated in this study.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"221-238"},"PeriodicalIF":1.7,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of Chlamydia spp., FIV, FeLV in Free-Roaming Cats in Slovakia.","authors":"Boris Vojtek, Peter Čechvala, Silvia Zemanová, Ľuboš Korytár, Marián Prokeš, Monika Drážovská, Patrícia Petroušková, Jana Kožiarská Tomčová, Anna Ondrejková","doi":"10.2147/VMRR.S465088","DOIUrl":"https://doi.org/10.2147/VMRR.S465088","url":null,"abstract":"<p><strong>Purpose: </strong>Free-roaming cats represent a potential reservoir of infectious diseases. The most common co-infections of free-roaming cats include mixed viral, bacterial, fungal, yeast and parasitic infections. This study focuses on the occurrence of <i>Chlamydia</i> spp. feline immunodeficiency virus (FIV), feline leukaemia virus (FeLV) and their co-infections. The diseases accompanied by immune suppression, such as FIV, create favourable conditions for the onset of other diseases and co-infections. The result of co-infection may be a higher susceptibility for other pathogens, as well as the occurrence of more severe clinical symptoms.</p><p><strong>Patients and methods: </strong>The study involved 168 (113♀ and 55♂) free-roaming adult cats during the years 2021-2022. All cats belonged to Slovak citizens with permanent residence in the Slovak Republic. Blood samples and swabs (Invasive EUROTUBO^® Collection sterile swab, Deltalab O8191 Rubí, Spain) from the conjunctival sac were taken from 168 cats to be later tested by PCR and ELISA methods. Statistical analysis was also performed.</p><p><strong>Results: </strong>The overall prevalence of <i>Chlamydia</i> spp. was 17.26%, of FIV 15.48%, and 5.95% of FeLV. The most significant finding in our study was 3.57% co-infection of FIV and <i>Chlamydia</i> spp. in tested cats.</p><p><strong>Conclusion: </strong>The observed prevalence of <i>Chlamydia</i> spp. FIV and FeLV indicates that the presence of these pathogens in populations of free-roaming cats is endemic.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"205-220"},"PeriodicalIF":1.7,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142334099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of Noninvasive Methemoglobin and Carboxyhemoglobin Measurements Using Pulse Co-Oximeter in Healthy Dogs.","authors":"Jiwoong Her, Justin Roh, Deborah A Keys","doi":"10.2147/VMRR.S465945","DOIUrl":"10.2147/VMRR.S465945","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the agreement between pulse co-oximeter and blood co-oximeter in measuring methemoglobin (MetHb) and carboxyhemoglobin (COHb) in healthy, awake dogs.</p><p><strong>Patients and methods: </strong>Forty-five healthy dogs were enrolled in the study. Pulse co-oximetry was performed using the Masimo Radical 7 Pulse Co-Oximeter with a Rainbow^® adhesive sensor. Simultaneously, venous blood samples were collected, and MetHb and COHb were immediately measured using a Stat Profile Prime Plus VET Critical Care Analyzer. Paired measurements of MetHb and COHb were evaluated via Spearman correlation, intra-class correlation (ICC), and Bland-Altman plots to evaluate the degree of agreement between the pulse co-oximeter and the blood co-oximeter.</p><p><strong>Results: </strong>A total of 45 paired MetHb and COHb measurements were collected. There was a weak correlation between the pulse co-oximeter and the blood co-oximeter readings. The correlation coefficients for MetHb and COHb were 0.0 (95% CI, -0.3 to 0.3) and 0.03 (95% CI, -0.27 to 0.32), respectively. The ICC indicated poor agreement between the pulse and blood co-oximeter for MetHb (ICC = 0.00, 95% CI: -0.12 to 0.15) and COHb (ICC = 0.03, 95% CI: -0.27 to 0.33). Bland-Altman plots revealed low mean bias but wide limits of agreement, indicating that the pulse co-oximeter overestimated MetHb by on average of 0.7% (P < 0.0001) (95% LoA: -0.5 to 2.0) and COHb by on average 0.2% (P = 0.59) (95% LoA: -4.6 to 5.0).</p><p><strong>Conclusion: </strong>Obtaining MetHb and COHb measurements with the Masimo Radical 7 Pulse Co-Oximeter is straightforward in healthy, awake dogs. However, the device does not provide accurate measurements compared to the blood co-oximeter, specifically in the range of MetHb and COHb in healthy dogs, based on the wide LoA.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"197-203"},"PeriodicalIF":1.7,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11334929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142010080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal Microbiota Transplantation in a Domestic Ferret Suffering from Chronic Diarrhea and Maldigestion-Fecal Microbiota and Clinical Outcome: A Case Report.","authors":"Sean J Ravel, Victoria M Hollifield","doi":"10.2147/VMRR.S449473","DOIUrl":"10.2147/VMRR.S449473","url":null,"abstract":"<p><p>This case report describes the effects of fecal microbiota transplantation (FMT) administered via enema in a 4-year-old spayed, champagne Domestic Ferret (<i>Mustela putorius furo</i>) with chronic diarrhea, maldigestion and weight loss. We aimed to establish a protocol for FMT as a novel therapeutic treatment for chronic diarrhea in domestic ferrets. We mapped the fecal microbiome by 16S rRNA gene amplicon sequencing to track the patient's fecal microbiota throughout the treatment and observation period. Initial oral FMTs were associated with temporary weight improvement but subsequent treatments, via enema and oral delivery, showed varied outcomes. Molecular analysis highlighted distinct gut microbiota composition profiles between the healthy donor and the diseased ferret. The diseased ferret initially exhibited high abundance of <i>Enterobacteriaceae, Escherichia</i>, and <i>Enterobacter</i>, which ultimately normalized to level like those found in the donor ferret. Overall, the gut microbiota of the recipient became more similar to the donor microbiota using a Yue-Clayton theta coefficients analysis. After a restoration of the gut microbiota and clinical improvement, the recipient's symptoms returned indicating that repeated FMTs might be required for long-term resolution of symptoms and complete restructuring of the gut microbiota. Future studies are warranted to map the microbiome of a larger population of domestic ferrets to investigate a potential correlation between fecal microbiota profiles and chronic/acute gastrointestinal disorders.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"171-180"},"PeriodicalIF":0.0,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11143982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended-Spectrum Beta-Lactamase Producing <i>Escherichia coli</i> in Raw Cow Milk At Selling Points and Determinants of Contamination in and Around Chencha, Southern Ethiopia.","authors":"Tomas Tonjo Torka","doi":"10.2147/VMRR.S454930","DOIUrl":"10.2147/VMRR.S454930","url":null,"abstract":"<p><strong>Introduction: </strong>Bacterial foodborne infections are a major public health concern globally, and the emergence of antimicrobial-resistant bacteria in food worsens the associated problems which are alarming issues. The current study aimed to isolate <i>E. coli</i>, determine antimicrobial resistance patterns, estimate the prevalence of extended-spectrum beta-lactamase-producing <i>E. coli</i>, and determinants of raw cow milk contamination at selling points in and around Chencha, Southern Ethiopia from January 2021 to April, 2022.</p><p><strong>Methods: </strong>A total of 384 milk samples were collected randomly using a cross-sectional study and processed in the laboratory for evaluation of microbial load, bacterial isolation, and antimicrobial susceptibility patterns. The determinants were assessed through a prepared questionnaire, and descriptive statistics and multivariable logistic regression analysis were performed using SPSS version 21.</p><p><strong>Results: </strong>Out of 384 milk samples analyzed for microbial loads, 5.3±1.68 log and 2.17±1.51 log CFU/mL respectively were the mean values of total viable count and total coliform count. A total of 118 (30.7%) samples were contaminated with <i>E. coli</i> of about 11.8% extended-spectrum beta-lactamase producers. Notably, 91 (77.1%) of isolates were multiple drug-resistant isolates. The multivariable logistic regression showed that variables of educational status of milk handlers, hand washing activities, nose picking habit of milk handlers, milk container cleaning activity, milk container type, physical abnormal milk checking status, and attended training on hygienic food handling practices type were significantly associated with the milk contamination in the study area.</p><p><strong>Conclusion: </strong>The results implied that the raw milk samples served in the study area contained bacterial pathogens and a higher microbial load than recommended standards. Our data also confirmed the presence of multiple drug resistant and extended-spectrum beta-lactamase producing <i>E. coli</i>. Therefore, a close follow-up and training of milk handlers is needed. Besides, consumers should be made aware of the risks of consuming raw milk.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"159-169"},"PeriodicalIF":0.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11112127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141088988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection and Serological Investigation of Newcastle Disease in Intensive, Semi-Intensive, and Backyard Production Systems in Central and Southwestern Areas of Ethiopia.","authors":"Bezina Arega Emeru, Dejene Getachew Dame, Haregawi Tesfaye Desta","doi":"10.2147/VMRR.S445261","DOIUrl":"10.2147/VMRR.S445261","url":null,"abstract":"<p><strong>Purpose: </strong>The purpose of this research is to detect Newcastle disease virus and to assess the seropositivity among backyard, semi-intensive, and intensive farms located in central and southwestern areas of Ethiopia.</p><p><strong>Material and methods: </strong>A total of 239 oropharyngeal and cloacal swab samples were collected from symptomatic birds found in Holeta, Burayu, Jimma towns as well as Seka Chekorsa and Nadhigibe woredas of Jimma Zone. In addition, ninety blood samples were collected from wing veins of unvaccinated birds found in the study areas of Jimma zone. Side-by-side information related to risk factors estimated to contribute to the susceptibility of the disease was collected by interviewing owners of sampled birds. Reverse transcription polymerase-chain reaction (RT-PCR) was conducted to detect NDV. Likewise, Enzyme-linked immunosorbent assay (ELISA) was performed to determine the seropositivity of ND.</p><p><strong>Results: </strong>The proportion of samples where NDV was detected was 24.6%. Similarly, 68.9% of the sampled birds were seropositive. It was observed that adult birds were more likely to encounter the disease than youngs (OR = 11.6; 95% CI: 4.0-33.3; P = 0.000). Birds owned by respondents who leave diseased birds in the flock were more likely infected (OR = 6.2; 95% CI: 1.8-21.2; P=0.004) as compared to those isolated and mode of disposal of dead chicken significantly affect exposure (OR = 0.13; 95% CI: 0.10-4.88; P = 0.044). Likewise, access to veterinary services highly likely reduces susceptibility to the disease (OR = 12.4; 95% CI: 3.2-46.9; P = 0.000). It was also found that birds farmed intensively were the most at risk (OR = 2.8; 95% CI: 0.58-13.71; P = 0.199).</p><p><strong>Conclusion: </strong>Detection of ND from a significant proportion of sampled birds and their high seropositivity percentage revealed the circulation of the virus in the study areas.</p>","PeriodicalId":75300,"journal":{"name":"Veterinary medicine (Auckland, N.Z.)","volume":"15 ","pages":"141-148"},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11090124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}