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Translational biophotonics Pub Date : 2021-08-01 DOI: 10.1002/tbio.202170004

引用次数: 0

Gas in scattering media absorption spectroscopy on small and large scales: Toward the extension of lung spectroscopic monitoring to adults 小尺度和大尺度散射介质中的气体吸收光谱：将肺部光谱监测扩展到成人

Translational biophotonics Pub Date : 2021-07-10 DOI: 10.1002/tbio.202100003

Yueyu Lin, P. Lundin, E. Krite Svanberg, K. Svanberg, S. Svanberg, A. Sahlberg

{"title":"Gas in scattering media absorption spectroscopy on small and large scales: Toward the extension of lung spectroscopic monitoring to adults","authors":"Yueyu Lin, P. Lundin, E. Krite Svanberg, K. Svanberg, S. Svanberg, A. Sahlberg","doi":"10.1002/tbio.202100003","DOIUrl":"https://doi.org/10.1002/tbio.202100003","url":null,"abstract":"Numerous natural materials are porous, contain free gas and are scattering light strongly. Scattering brings about a strong trapping of light and an associated prolonged transit time for photons through a medium. In contrast to the matrix materials, gas enclosures require very narrowband laser radiation for probing. We have in the present study used the gas in scattering media absorption spectroscopy method to study free oxygen in thin (cm) samples utilizing a tunable diode laser, while a pulsed dye laser was employed in corresponding measurements on larger samples, up to the meter scale. Time‐resolved spectroscopy was in both cases used to assess the temporal distribution of the detected photons, mapping the path lengths through the media, which ranged between few centimeters up to 100 m. This study explores the feasibility to extend recent successful monitoring of gases in neonatal infant lungs to the case of larger children or even adults, which could have very important applications, for example, in ventilator setting optimization for severely ill patients, suffering, for example, from SARS‐CoV‐2. The conclusion of our work is that this goal most realistically can be reached by applying intratracheal laser light illumination at the 1 W power level, employing a tapered amplifier, injected with a distributed feedback diode‐laser oscillator output and combined with wavelength‐modulation spectroscopy.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.202100003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48239811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Observation of the compatibility of stimulated Raman histology with pathology workflow and genome sequencing 刺激拉曼组织学与病理流程及基因组测序的相容性观察

Translational biophotonics Pub Date : 2021-06-28 DOI: 10.1002/TBIO.202000020

Barbara Sarri, R. Appay, S. Heuke, F. Poizat, F. Franchi, S. Boissonneau, F. Caillol, H. Dufour, D. Figarella-Branger, M. Giovaninni, H. Rigneault

引用次数: 1

Probing interneuronal cell communication via optogenetic stimulation 通过光遗传刺激探测神经元间的通讯

Translational biophotonics Pub Date : 2021-06-03 DOI: 10.1002/TBIO.202100002

P. Heeger, J. Harre, A. Warnecke, Dominik Mueller, S. Kalies, A. Heisterkamp

引用次数: 0

Disease activation maps for subgingival dental calculus identification based on intelligent dental optical coherence tomography 基于智能牙科光学相干断层扫描的龈下牙石识别疾病激活图

Translational biophotonics Pub Date : 2021-05-07 DOI: 10.1002/TBIO.202100001

Tien-Yu Hsiao, Yi-Ching Ho, Mei-Ru Chen, Shyh-Yuan Lee, Chia‐Wei Sun

引用次数: 3

Fourier‐transform infrared spectroscopy of biofluids: A practical approach 生物流体的傅立叶变换红外光谱：一种实用的方法

Translational biophotonics Pub Date : 2021-01-16 DOI: 10.1002/TBIO.202000025

A. G. Theakstone, Christopher Rinaldi, Holly J. Butler, James M. Cameron, L. Confield, Samantha H. Rutherford, A. Sala, Sayali Sangamnerkar, M. Baker

引用次数: 25

Issue Information 问题信息

Translational biophotonics Pub Date : 2020-11-01 DOI: 10.1002/tbio.201970008

引用次数: 0

Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time. 紧凑便携式多光子显微镜实时显示未处理肺肿瘤组织的组织病理学特征。

Translational biophotonics Pub Date : 2020-11-01 Epub Date: 2020-08-21 DOI: 10.1002/tbio.202000009

Laura M G van Huizen, Teodora Radonic, Frank van Mourik, Danielle Seinstra, Chris Dickhoff, Johannes M A Daniels, Idris Bahce, Jouke T Annema, Marie Louise Groot

{"title":"Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.","authors":"Laura M G van Huizen, Teodora Radonic, Frank van Mourik, Danielle Seinstra, Chris Dickhoff, Johannes M A Daniels, Idris Bahce, Jouke T Annema, Marie Louise Groot","doi":"10.1002/tbio.202000009","DOIUrl":"10.1002/tbio.202000009","url":null,"abstract":"During lung cancer operations a rapid and reliable assessment of tumor tissue can reduce operation time and potentially improve patient outcomes. We show that third harmonic generation (THG), second harmonic generation (SHG) and two-photon excited autofluorescence (2PEF) microscopy reveals relevant, histopathological information within seconds in fresh unprocessed human lung samples. We used a compact, portable microscope and recorded images within 1 to 3 seconds using a power of 5 mW. The generated THG/SHG/2PEF images of tumorous and nontumorous tissues are compared with the corresponding standard histology images, to identify alveolar structures and histopathological hallmarks. Cellular structures (tumor cells, macrophages and lymphocytes) (THG), collagen (SHG) and elastin (2PEF) are differentiated and allowed for rapid identification of carcinoid with solid growth pattern, minimally enlarged monomorphic cell nuclei with salt-and-pepper chromatin pattern, and adenocarcinoma with lipidic and micropapillary growth patterns. THG/SHG/2PEF imaging is thus a promising tool for clinical intraoperative assessment of lung tumor tissue.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"2 4","pages":"e202000009"},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.202000009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39278346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Prediction of pathological response to neo‐adjuvant chemoradiotherapy for oesophageal cancer using vibrational spectroscopy 振动光谱法预测食管癌症新辅助放化疗的病理反应

Translational biophotonics Pub Date : 2020-09-29 DOI: 10.1002/tbio.202000014

T. N. Q. Nguyen, A. Maguire, C. Mooney, N. Jackson, N. Lynam‐Lennon, Vicki Weldon, C. Muldoon, A. Maguire, D. O’Toole, N. Ravi, J. Reynolds, J. O’Sullivan, A. Meade

{"title":"Prediction of pathological response to neo‐adjuvant chemoradiotherapy for oesophageal cancer using vibrational spectroscopy","authors":"T. N. Q. Nguyen, A. Maguire, C. Mooney, N. Jackson, N. Lynam‐Lennon, Vicki Weldon, C. Muldoon, A. Maguire, D. O’Toole, N. Ravi, J. Reynolds, J. O’Sullivan, A. Meade","doi":"10.1002/tbio.202000014","DOIUrl":"https://doi.org/10.1002/tbio.202000014","url":null,"abstract":"In oesophageal cancer (OC) neo-adju-vant chemoradiotherapy (neoCRT) is used to debulk tumour size prior to surgery, with a complete pathological response (pCR) observed in approximately (cid:1) 30% of patients. Presently no predictive quantitative methodology exists which can predict response, in particular a pCR or major response (MR), in patients prior to therapy. Raman and Fourier transform infrared imaging were performed on OC tissue specimens acquired from 50 patients prior to therapy, to develop a computa-tional model linking spectral data to treatment outcome. Modelling sensitivities and specificities above 85% were achieved using this approach. Parallel in-vitro studies using an isogenic model of radioresistant OC supplied further insight into OC cell spectral response to ionising radiation where a potential spectral biomarker of radioresistance was observed at 977 cm − 1 . This work demonstrates that chemical imaging may provide an option for triage of patients prior to neoCRT treatment allowing more precise prescription of treatment.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.202000014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47867856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Highly sensitive lipid detection and localization in atherosclerotic plaque with a dual-frequency intravascular photoacoustic/ultrasound catheter. 使用双频血管内光声/超声导管对动脉粥样硬化斑块进行高灵敏度的脂质检测和定位。

Translational biophotonics Pub Date : 2020-08-01 Epub Date: 2020-05-12 DOI: 10.1002/tbio.202000004

Yingchun Cao, Mouhamad Alloosh, Michael Sturek, Ji-Xin Cheng

{"title":"Highly sensitive lipid detection and localization in atherosclerotic plaque with a dual-frequency intravascular photoacoustic/ultrasound catheter.","authors":"Yingchun Cao, Mouhamad Alloosh, Michael Sturek, Ji-Xin Cheng","doi":"10.1002/tbio.202000004","DOIUrl":"https://doi.org/10.1002/tbio.202000004","url":null,"abstract":"Intravascular photoacoustic/ultrasound (IVPA/US) is an emerging hybrid imaging modality that provides specific lipid detection and localization, while maintaining co-registered artery morphology, for diagnosis of vulnerable plaque in cardiovascular disease. However, current IVPA/US approaches based on a single-element transducer exhibit compromised performance for lipid detection due to the relatively low contrast of lipid absorption and conflicting detection bands for photoacoustic and ultrasound signals. Here, we present a dual-frequency IVPA/US catheter for highly sensitive detection and precision localization of lipids. The low frequency transducer provides enhanced photoacoustic sensitivity, while the high frequency transducer maintains state-of-the-art spatial resolution for ultrasound imaging. The boosted capability of IVPA/US imaging enables a multi-scale analysis of lipid distribution in swine with coronary atherosclerosis. The dual-frequency IVPA/US catheter has a diameter of 1 mm and flexibility to easily adapt to current catheterization procedures and is a significant step toward clinical diagnosis of vulnerable plaque.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"2 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/tbio.202000004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41169957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10