Theriogenology wild最新文献

{"title":"Erratum to “The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: embryo development and nuclear gene expression” Theriogenol. Wild 1 (2022) 100001","authors":"Luciana M. Melo , Sâmara B. Silva , Lívia C. Magalhães , Jenin V. Cortez , Satish Kumar , José M.B. Duarte , Luciana D. Rola , Maiana S. Chaves , Vicente J.F. Freitas","doi":"10.1016/j.therwi.2023.100066","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100066","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100066"},"PeriodicalIF":0.0,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X2300051X/pdfft?md5=98fe1839ca4c31fb74c5a49e44599a19&pid=1-s2.0-S2773093X2300051X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138501830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"That was then, this is now – Over two decades of progress in rhinoceros reproductive science and technology","authors":"Terri L. Roth","doi":"10.1016/j.therwi.2023.100065","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100065","url":null,"abstract":"<div><p>Despite dipping to perilously low numbers over the past century, all five rhinoceros (rhino) species still survive in the wild with four also in managed breeding programs. These managed populations have been essential for advancing rhino reproductive science and technology. Despite a plethora of challenges and the incremental nature of sound science, researchers have made significant progress over the past quarter century in broadening our knowledge of rhino reproduction, developing new technologies, and expanding the scope of existing research tools. When we compare the state of this scientific field a quarter century ago to where it stands today, there is much to celebrate. For example, at the turn of the century, the Sumatran rhino breeding program had failed to produce a single calf, the first rhino artificial insemination (AI) procedures had just been described, but no pregnancies had been documented, and <em>in vitro</em> fertilization (IVF) had not succeeded in any rhino species. As we reach the end of 2023, 8 Sumatran rhino calves have been born, a total of 17 white and greater one-horned rhino calves have been produced by AI, and 51 white rhino IVF embryos have developed into blastocysts. Furthermore, several theories based on the evidence available at that time have been disproven as additional scientific data have deepened our knowledge and understanding. However, many unanswered questions still exist, and reproductive technologies require refinement, development, or application to additional rhino species, so plenty of challenges remain on the landscape for future generations of rhino reproductive scientists to conquer.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100065"},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X23000491/pdfft?md5=eec387d3d71065fcb0c0a7e81cedcc22&pid=1-s2.0-S2773093X23000491-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138501769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of water temperature and hybridization on embryonic development and gametogenesis of two species of Amazonian tetra","authors":"Saynara Miranda ,&nbsp;Ludmylla Santos ,&nbsp;Suianny Chaves ,&nbsp;Bianca Lima ,&nbsp;Jeane Rodrigues ,&nbsp;Maria Rosa-Silva ,&nbsp;Hadda Tercya ,&nbsp;Paulo Jesus ,&nbsp;Eduardo Albuquerque ,&nbsp;Caio Maximino ,&nbsp;Diógenes Siqueira-Silva","doi":"10.1016/j.therwi.2023.100051","DOIUrl":"10.1016/j.therwi.2023.100051","url":null,"abstract":"<div><p>The study of embryonic development in fish and how water temperature affects the life stage in this group is of pivotal importance to support works that aim at the conservation, management, and recovery of endangered or high economic valuable species. It allows a better evaluation of the species and the identification of morphophysiological changes related to the environmental factor in question. In addition, hybridization emerges as a biotechnological tool that may be applied as a technique for the creation of sterile animals that can be used as recipients for the germ cell transplantation approach, thus contributing to the conservation of different fish species. Based on this, we artificially reproduced two Amazonian fish species, <em>Astyanax bimaculatus,</em> and <em>Moenkhausia oligolepis</em>, and the resulting offspring of each species had their embryonic development followed at room temperature (25 °C) and at high temperature (30 °C). In addition, we performed interspecific crossbreeding between the species, and the offspring were also incubated and observed under the above conditions. The results showed that the high temperature accelerates the embryonic development of both, the offspring from <em>A. bimaculatus</em> and from hybridization. However, no larvae survived. Regarding <em>M. oligolepis,</em> the embryos did not develop, dying at 4:40 post-fertilization. For the hybridization, only the crossbreeding between females of <em>A. bimaculatus</em> and males of <em>M. oligolepis</em> resulted in normal larvae. They presented their own morphological characteristics, showing no total or partial characteristics from the parents, besides presenting atrophied and sterile gonads. Heat waters were proven to compromise the embryonic development of both Amazonian species. On the other side, the hybridization between those two Amazonian species has shown to be a tool with great potential for sterilization and the development of individuals suitable for use as surrogate breeders aiming for the ecological maintenance of various fish species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48824640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal bone development in the black agouti (Dasyprocta fuliginosa) determined by ultrasound","authors":"Alana Lorena Gonçalves Pombo ,&nbsp;Frederico Ozanan Barros Monteiro ,&nbsp;Gessiane Pereira da Silva ,&nbsp;Thyago Habner de Souza Pereira ,&nbsp;Sandy Estefany Rodrigues de Matos ,&nbsp;Ana Rita de Lima ,&nbsp;Hani Rocha El Bizri ,&nbsp;João Valsecchi do Amaral ,&nbsp;Carlos López Plana ,&nbsp;Pedro Mayor","doi":"10.1016/j.therwi.2023.100029","DOIUrl":"10.1016/j.therwi.2023.100029","url":null,"abstract":"<div><p>This study describes the bone development during the intrauterine phase of the black agouti (<em>Dasyprocta fuliginosa</em>), discussing its relationship with the species’ adaptive strategies and comparing it with other precocial and altricial species. We analyzed 33 conceptuses (four embryos and twenty-nine fetuses) obtained through collaboration with local hunters in the Amazon. Mineralization measurements of the axial and appendicular skeletons were performed by ultrasonography using a 10–18 MHz linear transducer. The chronological order of occurrence of mineralization in relation to the total dorsal length (TDL) and to the percentage of the total gestational period (GP) was: skull, ribs, vertebral bodies, clavicle, scapula, humerus, radius, ulna, ilium, ischium, femur, tibia, and fibula (TDL = 8.2 cm, 48 % GP); metacarpi, metatarsi and pubis (TDL = 9 cm, 51 % GP); thoracic and pelvic limb phalanges (TDL = 13.2 cm, 65 % GP); carpus (TDL = 15.10 cm, 72 % GP) and distal row of tarsus (TDL = 19.6 cm, 87 % GP). Mineralization of the patella was not observed in any advanced fetus (fetus with&gt; 80 % GP). Regarding secondary ossification centers, the first signs of mineralization were observed in the distal epiphysis of the radius, distal epiphysis of the femur, and proximal and distal epiphysis of the tibia (TDL = 13.2 cm, 65 % GP). Fetuses at birth (TDL &gt; 21.5 cm, 93.5 % GP) showed mineralization in all primary centers, and in most secondary ossification centers. Black agouti neonates have a high level of precociality with well-developed skeletal system at birth, which promotes independent postnatal locomotion and dexterity to manipulate and forage in search of food. Our results can contribute to the monitoring of bone development in other wild species, providing parameters for the identification of gestational age and serving as a model for comparisons between precocial and altricial mammals, ultimately helping understand life history strategies in different species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100029"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49389922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring estrous cycle in Brazilian dwarf brocket deer (Mazama nana) by fecal progestagen metabolites and behavior","authors":"Geissiane Neves Toledo ,&nbsp;David Javier Galindo ,&nbsp;Mariana de Britto Abrahão ,&nbsp;Katherinne Maria Spercoski ,&nbsp;José Maurício Barbanti Duarte ,&nbsp;Nei Moreira","doi":"10.1016/j.therwi.2022.100016","DOIUrl":"10.1016/j.therwi.2022.100016","url":null,"abstract":"<div><p>Data on the reproductive physiology of the Brazilian dwarf brocket deer (<em>Mazama nana</em>) is still scarce in many aspects, such as its reproductive cycle and endocrine pattern. Therefore, this study aimed to characterize the estrous cycle (length and fecal progestagen metabolites -FPM- profile) in captive females of <em>M. nana</em>, by non-invasive hormonal monitoring. The study was performed with females (n = 4), housed at two different facilities: 1) in a collective enclosure at the Danilo José Galafassi Municipal Zoo, in Cascavel, Paraná, Brazil, (n = 3). 2) in an individual pen at the Deer Research and Conservation Center (n = 1), from UNESP, Jaboticabal, São Paulo, Brazil. Fecal samples were collected three times a week during two complete estrous cycles, based on behavioral observations. FPM profiles were analyzed by enzyme immunoassay (EIA). The estrous cycle length was defined as the intervals between behavioral estrus observations and fecal hormonal monitoring. The mean duration (± SEM) of the estrous cycle was 23.9 ± 0.6 days, and the mean duration of behavioral estrus was 1.6 ± 0.3 days. Mean concentrations (± SEM) of FPM for the luteal phase and inter-luteal phase were 6183.0 ± 2268.7 ng/g and 754.5 ± 216.1 ng/g, respectively.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100016"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42001418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different anesthetic protocols on erection, ejaculation and sperm parameters in red-rumped agoutis (Dasyprocta leporina) subjected to electroejaculation","authors":"Amara Gyane Alves de Lima ,&nbsp;Maria Gláucia Carlos de Oliveira ,&nbsp;Isabelle de Oliveira Lima ,&nbsp;Kathryn Nóbrega Arcoverde ,&nbsp;Lívia Batista Campos ,&nbsp;Andreia Maria da Silva ,&nbsp;Thibério de Souza Castelo ,&nbsp;Moacir Franco de Oliveira ,&nbsp;Alexandre Rodrigues Silva ,&nbsp;Valéria Veras de Paula","doi":"10.1016/j.therwi.2023.100020","DOIUrl":"10.1016/j.therwi.2023.100020","url":null,"abstract":"<div><p>With the aim of contributing to the development of assisted reproduction for red-rumped agouti germplasm conservation, we compared different anesthetic protocols for electroejaculation in agoutis in terms of anesthetic parameters, functions of erection and ejaculation, and semen metrics. Ten sexually mature males underwent electroejaculation with 15-day intervals according to the following anesthetic protocols: Ketamine/xylazine, Ketamine/dexmedetomidine, Ketamine/xylazine/epidural anesthesia or Azaperone/meperidine/ketamine/xylazine plus epidural anesthesia. The following variables were evaluated: induction latency period, anesthetic recovery, and duration of epidural anesthesia. Regarding the reproductive variables, erection, the stimulation cycle in which ejaculation occurred, and the semen characteristics were observed. All the protocols were efficient for providing adequate induction of latency (ranging from 1.46 to 13.14 min) and recovery (average 2 h) periods. The use of a premedication did not influence the duration of epidural anesthesia (P&lt;0.05). The administration of an azaperone/meperidine-based premedication prior to epidural anesthesia promoted an increase in the agouti erection process when compared to the other protocols (P &lt; 0.05). All the protocols were efficient in stimulating ejaculation (100 %), even though most ejaculates were aspermic (85 %). The presence of sperm was only observed in 30 % of the animals anesthetized with ketamine/xylazine, 20 % with ketamine/dexmedetomidine, and 10 % with Ketamine/xylazine/epidural anesthesia. Ketamine/xylazine provided a lower volume (201.7±173.4 vs. 810.0±127.3 µL) and greater sperm concentration (270.0±200 vs. 25.0±15.0 × 10⁶ sperm/mL) than Ketamine/dexmedetomidine (P &lt; 0.05). In conclusion, we suggest the use of the standard anesthetic protocol based on the Ketamine/xylazine combination to obtain sperm in red-rumped agouti.</p></div><div><h3>Data availability statement</h3><p>The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100020"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49489913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reimmunization intervals for application of GnRH immunocontraceptive vaccine (GonaCon-Equine) in free-roaming horses (Equus ferus caballus) using syringe darts","authors":"Dan L. Baker ,&nbsp;Blake E. McCann ,&nbsp;Jenny G. Powers ,&nbsp;Nathan L. Galloway ,&nbsp;Jason E. Bruemmer ,&nbsp;Melissa A. Thompson ,&nbsp;Terry M. Nett","doi":"10.1016/j.therwi.2023.100061","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100061","url":null,"abstract":"<div><p>Management of overabundant free-roaming horse (<em>Equus ferus caballus</em>) populations has become a divisive and challenging effort for natural resource managers worldwide. Controlling these populations requires land managers to balance socio-political issues, multiple use management concepts, and endearing affection of horses to some stakeholders against negative ecological alterations created by excessive densities of these animals. One potential solution to this dilemma is controlling the fertility of female horses. During 2013–2020, research was conducted to develop and test a prototype dart configuration system to deliver an effective dose of GonaCon-Equine to free-roaming horses. This remote dart delivery system was demonstrated to be safe and reliable in free-ranging horses with a 90% (71/79) success rate for first attempts and provided the opportunity to design an experiment to determine the long-term effectiveness, reversibility, and physiological side effects of different reimmunization schedules. Five experimental groups of mares were established consisting of a control (n = 25), and a 4-yr reimmunization interval (n = 25) of mares from a previous experiment that were treated with this same vaccine by hand injection in 2013. Additional booster intervals of horses were (2-yr (n = 11), 1-yr (n = 15), and 0.5-yr (n = 14) and vaccinated by remote dart delivery during 2013–2016. Weekly ground observations were utilized to estimate foaling proportions, foal survival, body condition, and injection site reactions related to treatment groups. All GonaCon-Equine booster intervals reduced (<em>P ≤</em> 0.001) foaling proportions between treated and control mares for all years of the experiment. Effective contraception was also achieved when GonaCon-Equine was applied by remote dart delivery at post-primary intervals shorter than 4 years. Reversibility of GonaCon-Equine immunization occurred in all treatment groups and averaged 0.40 (26/65). The only detectable adverse side effect of vaccination was intramuscular swelling observed in 89% (115/129) of mares and occasional draining abscesses at the site of vaccine injection. However, regardless of method of vaccine delivery (hand vs dart), none of these mares displayed any evidence of lameness, altered gait, or abnormal range of movement during the 6 years of this study. These results support previous findings that effective contraception of free-roaming horses will require a primary vaccination followed by reimmunization but that timing from 6 months to 4 years result in similar success and together can achieve greater reduction in population growth rates.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":"Article 100061"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49709435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development – A model for wild felids","authors":"M. Bashawat ,&nbsp;B.C. Braun ,&nbsp;K. Müller ,&nbsp;B.P. Hermann","doi":"10.1016/j.therwi.2023.100031","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100031","url":null,"abstract":"<div><p>Molecular characterisation of testicular cells is a pivotal step towards a profound understanding of spermatogenesis and developing assisted reproductive techniques (ARTs) based on germline preservation. To enable the identification of testicular somatic and spermatogenic cell types in felids, we investigated the expression of five molecular markers at the protein level in testes from domestic cats (<em>Felis catus</em>) at different developmental phases (prepubertal, pubertal I and II, postpubertal I and II) classified by single-cell ploidy analysis. Our findings indicate a prominent co-labelling for two spermatogonial markers, UCHL1 and FOXO1, throughout postnatal testis development. Smaller subsets of UCHL1 or FOXO1 single-positive spermatogonia were also evident, with the FOXO1 single-positive spermatogonia predominantly observed in prepubertal testes. As expected, DDX4+ germ cells increased in numbers beginning in puberty, reaching a maximum at adulthood (post-pubertal phase), corresponding to the sequential appearance of labelled spermatogonia, spermatocytes and spermatids. Furthermore, we identified SOX9+ Sertoli cells and CYP17A1+ Leydig cells in all of the developmental groups. Importantly, testes of African lion (<em>Panthera leo</em>), Sumatran tiger (<em>Panthera tigris sumatrae</em>), Chinese leopard (<em>Panthera pardus japonesis</em>) and Sudan cheetah (<em>Acinonyx jubatus soemmeringii</em>) exhibited conserved labelling for UCHL1, FOXO1, DDX4, SOX9 and CYP17A1. The present study provides fundamental information about the identity of spermatogenic and somatic testicular cell types across felid development that will be useful for developing ART approaches to support endangered felid conservation.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49775827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the iSperm for assessing rhinoceros Sperm","authors":"Louisa A. Rispoli,&nbsp;Terri L. Roth","doi":"10.1016/j.therwi.2023.100048","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100048","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49709376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian ultrasound analysis for developing temporal and spatially explicit hormone regimens for induced ovulation and egg deposition in the tiger salamander (Ambystoma tigrinum)","authors":"Ruth M. Marcec-Greaves ,&nbsp;Carrie K. Kouba ,&nbsp;Scott T. Willard ,&nbsp;Andy J. Kouba","doi":"10.1016/j.therwi.2023.100038","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100038","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49782406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}