Theriogenology wild最新文献

{"title":"Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm","authors":"Pedro Nacib Jorge-Neto ,&nbsp;Fabio de Moraes Francisco ,&nbsp;Mario Davi Dias Carneiro ,&nbsp;Sergio Ricardo Brito Santos ,&nbsp;Letícia Alecho Requena ,&nbsp;Sofia Dressel Ramos ,&nbsp;Matheus Felix de Goés ,&nbsp;Rafael Franco Valle ,&nbsp;Fabiana Lucia André Padilha ,&nbsp;Helen Colbachini ,&nbsp;Rafael Caprioli Gutierrez ,&nbsp;Larissa Schneider Brandão Souza ,&nbsp;Verônica Takatsuka Manoel ,&nbsp;Laura Chrispim Reisfeld ,&nbsp;Thyara Deco-Souza ,&nbsp;Roberta Ferreira Leite ,&nbsp;Cristiane Schilbach Pizzutto","doi":"10.1016/j.therwi.2024.100091","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100091","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>One-third of elasmobranch species are threatened with extinction, and the precise assessment of their sperm parameters and quality is one stage in the development of reproductive technology for animal conservation.</p></span></li></ul><ul><li><span>2.</span><span><p>Computer-Assisted Sperm Analysis (CASA) is the gold standard for sperm analysis; however, there were not one setup established for elasmobranchs.</p></span></li></ul><p></p><ul><li><span>3.</span><span><p>This study presents the description of a specific setup developed for elasmobranch semen analysis using the CASA system.</p></span></li></ul><ul><li><span>4.</span><span><p>In addition, it provides a gold-standard technique protocol for elasmobranch sperm quality assessment.</p></span></li></ul><p></p><ul><li><span>5.</span><span><p>The described setup presented an accuracy of more than 90 percent in fourteen distinct species of freshwater and marine sharks, stingrays, and rays, failing only when there were two or more bundled sperm.</p></span></li></ul></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000229/pdfft?md5=e82e59bee8065b6a7446d1f238acb25b&pid=1-s2.0-S2773093X24000229-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141090281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive health assessments in female polar bears: Anatomy, pathology, and diagnostic techniques","authors":"Erin Curry,&nbsp;Julie Barnes,&nbsp;Elizabeth Donelan,&nbsp;Jessye Wojtusik ,&nbsp;Victoria Kennedy,&nbsp;Emily E. Virgin,&nbsp;Megan Philpott,&nbsp;Terri L. Roth","doi":"10.1016/j.therwi.2024.100105","DOIUrl":"10.1016/j.therwi.2024.100105","url":null,"abstract":"<div><p>The reproductive success of polar bears (<em>Ursus maritimus</em>) in U.S. zoos is poor and the underlying causes of reproductive failure remain elusive. It is evident that new methodologies for evaluating fertility are needed, along with baseline information about species-specific reproductive anatomy and physiology. Between 2012 and 2024, 25 examinations of 17 female polar bears were conducted opportunistically, and included vulvar measurements, vaginal cytology, vaginoscopy, hysteroscopy with endometrial biopsy, and/or ultrasonography. Additionally, reproductive tracts (n = 10) harvested post-mortem were examined shortly after collection, allowing for inspection of anatomy <em>ex vivo</em>. The outcomes of these activities yielded detailed measurements and descriptions of reproductive anatomy. Vaginal cytology patterns demonstrated shifts in vaginal epithelial cell type associated with estrus and vaginoscopy enabled systematic evaluation of internal reproductive structures, including the vestibule, vestibulo-vaginal junction, urethral os, vaginal canal, and cervix. Hysteroscopy allowed visualization of the uterine endometrium and facilitated endometrial biopsy collection. Additionally, ultrasonography was utilized to identify ovaries in several bears, offering insights into ovarian dynamics and follicular development. Several abnormalities were noted during examinations of both <em>in vivo</em> and <em>ex vivo</em> tracts; these included clitoral hypertrophy, vaginal strictures, uterine polyps, endometritis, and cystic hyperplasia, although it is unclear if these aberrations significantly impacted fertility. Findings contribute to a better understanding of polar bear reproductive anatomy, highlight the utility of diagnostic techniques for monitoring reproduction, and elucidate potential causes of reproductive failure in polar bears.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100105"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000369/pdfft?md5=161ddb3016568070815be217fcfc2f76&pid=1-s2.0-S2773093X24000369-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141949782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold dogs: Sperm freezing, artificial insemination & non-invasive monitoring tools to facilitate a hybrid conservation management approach for endangered African wild dogs","authors":"Damien Boyd Bertrand Paul Paris ,&nbsp;Pia Riddell ,&nbsp;Carolynne Joone ,&nbsp;Morné de la Rey ,&nbsp;Andre Ganswindt ,&nbsp;Monique Christina Johanna Paris","doi":"10.1016/j.therwi.2024.100073","DOIUrl":"10.1016/j.therwi.2024.100073","url":null,"abstract":"<div><p>Given the ongoing fragmentation and decline of suitable habitat coupled with recurring disease outbreaks and pack eradications, a major rethink is required to manage the long-term genetic diversity of the endangered African wild dog. Competing financial demands in developing countries mean that conservation strategies need to yield the biggest genetic gains for the smallest economic cost. As such, we propose the development of a hybrid conservation approach to the metapopulation management of African wild dogs, which augments assisted dispersal and natural breeding initiatives with genetic gains afforded by sperm banking and artificial insemination. We discuss how such an approach would address current challenges faced by the species in the context of its unique social biology and how this could integrate with existing conservation management interventions. Moreover, during a period spanning nearly 20 years, we have developed many of the supporting reproductive technologies required to maximise genetic diversity of African wild dogs, in addition to a suite of non-invasive management tools to quantify, monitor and manipulate behaviour, vocalisations, hormones for reproductive function and responses to stressors, as well as immune function to improve the social cohesion, health and reproduction of packs.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000047/pdfft?md5=f8e155922e616043e1dd1f84bcf3e083&pid=1-s2.0-S2773093X24000047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139540629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized heterologous in vitro fertilization with Iberian ibex sperm and domestic goat oocytes","authors":"Nuria Martínez de los Reyes,&nbsp;Melissa Carvajal-Serna,&nbsp;Inés Flores-Borobia,&nbsp;Pilar Marigorta,&nbsp;Patricia Peris-Frau,&nbsp;Julián Santiago-Moreno,&nbsp;Pablo Bermejo-Álvarez,&nbsp;Priscila Ramos-Ibeas","doi":"10.1016/j.therwi.2024.100075","DOIUrl":"10.1016/j.therwi.2024.100075","url":null,"abstract":"<div><p>Assisted reproductive technologies are key to maintain genetic stocks of endangered wild species, such as the Iberian ibex (<em>Capra pyrenaica</em>). Due to the low availability of ibex ovaries, heterologous in vitro fertilization (IVF) with domestic goat (<em>Capra hircus</em>) oocytes constitutes an excellent alternative to determine the fertilization capacity of ibex sperm doses. The aim of this study was to optimize heterologous ibex-goat IVF procedures by testing two different IVF media (TALP and SOF) and to determine whether estrous sheep serum (ESS) is required for fertilization. We found that TALP medium provides optimal conditions to conduct heterologous ibex-goat IVF, yielding blastocyst rates above 50%, and that supplementation with ESS is not required. No differences were found in embryo quality between embryos fertilized in TALP, SOF alone, or SOF supplemented with 2 or 20% ESS, based on the analysis of cell lineages development at day (D) 8 and D10 of development. Optimized heterologous ibex-goat IVF, together with embryo quality assessment through lineages development analysis, constitute an excellent system to assess fertilization capacity of ibex sperm doses, and opens the possibility of performing homologous in vitro embryo production in this species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000060/pdfft?md5=4c2b24959467dc67956c5dd1d32e3db1&pid=1-s2.0-S2773093X24000060-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139637945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm collection in rhinoceros via urethral catheterization","authors":"Anneke Moresco ,&nbsp;Justine K. O’Brien ,&nbsp;Jessye Wojtusik ,&nbsp;Monica A. Stoops","doi":"10.1016/j.therwi.2024.100090","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100090","url":null,"abstract":"<div><p>Assisted reproductive technologies are playing an ever-increasing role in the management of <em>ex situ</em> rhinoceros populations. Traditionally, sperm collection in rhinos is conducted via electroejaculation (EEJ) or gamete rescue. Sperm collection via urethral catheterization (UC) is gaining popularity in wildlife reproductive sciences as it requires less specialized equipment, is faster, and less stimulatory than EEJ. This study investigated the use of UC to collect sperm from Southern white (<em>Ceratotherium simum simum;</em> n = 3) and Eastern black (<em>Diceros bicornis michaeli;</em> n = 6) rhinoceros as an alternative or complementary to collection via EEJ. UC samples (n = 14) exhibited high sperm concentration (1.6 ± 0.49 ×10⁹ sperm/mL) and low volume (0.98 ± 0.26 mL). Samples obtained via EEJ (n = 11) were of moderate sperm concentration (0.14 ± 0.05 ×10⁹ sperm/mL) and high volume (30.6 ± 7.2 mL). UC samples were processed for cryopreservation in OptiXcell extender. Motility of UC sperm decreased post-thaw (56 ± 2.4%) over pre-freeze (77.1 ± 3.2%; <em>P</em> &lt; 0.05), with longevity data consistent to prior results published for sperm collected via EEJ. These results suggest sperm quality and cryo-survival are not impacted by the collection method. Since EEJ was conducted after UC, sperm parameters could not be compared between the two methods. Results indicate that UC is a viable alternative to EEJ for sperm collection in rhinoceros.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000217/pdfft?md5=1067a9ffa4ac0ee4d8c270d00f4db441&pid=1-s2.0-S2773093X24000217-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring and exploring variations of serum anti-Müllerian hormone (AMH) in female fishing cat (Prionailurus viverrinus) and leopard cat (Prionailurus bengalensis)","authors":"Sittat Chumsri ,&nbsp;Itti Boonorrana ,&nbsp;Junpen Suwimonteerabutr ,&nbsp;Wanlaya Tipkantha ,&nbsp;Ampika Thongphakdee ,&nbsp;Pierre Comizzoli ,&nbsp;Kaywalee Chatdarong","doi":"10.1016/j.therwi.2024.100115","DOIUrl":"10.1016/j.therwi.2024.100115","url":null,"abstract":"<div><div>Serum concentration of anti-Müllerian hormone (AMH) is a way to estimate the ovarian reserve in many species including domestic cats. It could therefore become an important indicator to manage breeding of wild cat species in captivity. This study focused on 1) validating the AMH Gen II ELISA test, and 2) exploring the relationship between serum AMH with age and estrus stages in fishing cats (<em>Prionailurus viverrinus</em>) and leopard cats (<em>Prionailurus bengalensis</em>). Blood samples were collected from 11 female fishing cats (5.7 ± 3.2 years old) and 8 female leopard cats (8.1 ± 1.8 years old) subjected to routine annual health check in zoo facilities. The stage of the estrus cycle was estimated from animal history, abdominal sonography and vaginal cytology. Serum AMH was measured by AMH Gen II ELISA. Intra-assay CV and inter-assay CV were 4.11 % and 5.52 %, respectively. Validation of the AMH Gen II ELISA by the linearity of sample dilution in fishing cats and leopard cats was confirmed. In fishing cats, r² equalled 0.998 and percentage recovery was 83–101 %. In leopard cats, r² equalled 0.999 and percentage recovery was 96–104 %. Serum AMH concentration ranged from 0.17 to 30.53 ng/mL (averaged 14.88 ± 10.03 ng/mL) in fishing cats and 1.25 to 13.52 ng/mL (averaged 6.25 ± 4.69 ng/mL) in leopard cats. There was no significant relationship between serum AMH concentration and age in both fishing cats (r = − 0.556, <em>P</em> = 0.075) and leopard cats (r = 0.010, <em>P</em> = 0.982). Likewise, there was no difference in AMH concentrations between estrus stage and other stages in both fishing cats (<em>P</em> = 0.069) and leopard cats (<em>P</em> = 0.599). In conclusion, because the limited sample size and the lack of precise information on the estrus phase, the AMH Gen II ELISA shows promising potential for detecting concentrations that may be correlated with age, estrus cycle, and ovarian reserve in future studies.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen collection and baseline semen characteristics of the king quail (Synoicus chinesis)","authors":"Isabella Wong ,&nbsp;Robert Doneley ,&nbsp;Stephen Johnston","doi":"10.1016/j.therwi.2024.100117","DOIUrl":"10.1016/j.therwi.2024.100117","url":null,"abstract":"<div><div>Assisted breeding technology has important management implications for avian conservation and aviculture but species-specific reproductive information is lacking. This study aimed to develop a successful semen collection for the king quail (<em>Synoicus chinesis</em>) in order to establish baseline seminal characteristics and to explore the effect of flock density on seminal quality. A total of 19 quail consisting of 2 groups of 5 and 4 and 1 group of 10 were housed in 3 separate aviaries located at the University of Queensland, Gatton, Australia during Spring. Morphometrics and morphology (normal and abnormal) of the sperm cells were also described by phase contrast and differential interference microscopy. Semen was sucessfully collected by cloacal massage from 90 % of attempts with only minor evidence of faecal or urate contamination. No significant differences in seminal characteristics were found with respect to bird housing density. Mean ± SEM seminal characteristics for 103 ejaculates included volume (1.6 ± 0.02 μL), sperm concentration (8.1 ± 0.5 × 10⁹ mL^–1), progressive sperm motility (50.0 ± 0.04 %), rate of sperm motility, (2.9 ± 0.13), pH (6.7 ± 0.2), the percentage of live sperm (88.0 ± 0.01 %) and the percentage of total abnormal sperm morphology (21.0 ± 0.03 %). Cloacal massage proved to be a highly successful technique to collect king quail semen and the seminal characteristics reported here are the future basis of assessing fertility and the application of assisted breeding in the species.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen collection and characterization of normative reproductive traits in free-ranging ocelots (Leopardus pardalis) and bobcats (Lynx rufus) in southern Texas","authors":"Ashley M. Reeves ,&nbsp;William F. Swanson ,&nbsp;Clayton D. Hilton ,&nbsp;Tyler A. Campbell ,&nbsp;Landon Schofield ,&nbsp;Michael E. Tewes ,&nbsp;Hilary M. Swarts ,&nbsp;Amy Miller ,&nbsp;Cary Springer ,&nbsp;Debra L. Miller","doi":"10.1016/j.therwi.2024.100107","DOIUrl":"10.1016/j.therwi.2024.100107","url":null,"abstract":"<div><p>Decreased genetic diversity and possible inbreeding depression have recently been documented in the last wild ocelot (<em>Leopardus pardalis</em>) population in the United States. One consequence of inbreeding depression in felids may be reduced semen quality which can adversely affect reproductive potential. Detailed assessments of reproductive parameters in wild individuals and populations can be conducted using assisted reproductive technologies, such as semen collection and analysis. For most felid species, semen has traditionally been collected via electroejaculation (EEJ<span><span>²</span></span>); however, an alternative method has been developed using alpha-2 agonist drugs to induce direct sperm release into the urethra, allowing collection by catheterization without requiring specialized equipment. The goal of this study was to characterize normative reproductive traits in free-ranging ocelots and co-occurring bobcats (<em>Lynx rufus</em>) in southern Texas and assess the effectiveness of urethral catheterization (UC<span><span>³</span></span>) for semen recovery in both species. For semen collection, free-ranging cats were live-captured and anesthetized using intramuscular ketamine and medetomidine/dexmedetomidine (alpha-2 agonist) with UC conducted 20–40 minutes post-induction. In ocelots only, EEJ was subsequently performed if UC failed to recover a viable sample. Semen collection was attempted in 31 felids (n=9 ocelots; n=22 bobcats), with sperm recovery by UC in seven of nine ocelots (78 %) and 14 of 22 bobcats (66 %), and by EEJ in four of five ocelots (80 %). For ocelots, the percentage of primary morphologic abnormalities was higher (p&lt;0.001) for UC (47.75 ± 6.7; mean ± SEM) compared to EEJ (9 ± 2.7) but percent normal morphology (MORPH) did not differ between UC and EEJ (p=0.218). In wild ocelots, seminal parameters appeared lower relative to historical values reported for zoo-managed ocelots, possibly related to reduced heterozygosity. In wild bobcats, seminal traits were inferior to those of ocelots but similar to reports for other zoo-managed <em>Lynx</em> species. In conclusion, detailed male reproductive traits have been characterized for the first time in wild, free-ranging ocelots and bobcats in southern Texas. Although UC allowed semen recovery for assessment of seminal traits in both species, EEJ produced higher quality samples in ocelots when applied after UC while also mitigating the adverse impact of urine contamination observed frequently with both collection methods.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100107"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000382/pdfft?md5=4b9c4b85d585cf1c42bedc153e70d286&pid=1-s2.0-S2773093X24000382-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142117685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epididymal spermatozoa from domestic cats in assisted reproduction biotechniques: Perspectives for wild felid applications","authors":"Silmara Leticia Gonçalves Lima ,&nbsp;Danuza Leite Leão ,&nbsp;Adriana Novaes dos Reis ,&nbsp;Josye Bianca Santos ,&nbsp;Regiane Rodrigues dos Santos ,&nbsp;Sheyla Farhayldes Souza Domingues","doi":"10.1016/j.therwi.2024.100116","DOIUrl":"10.1016/j.therwi.2024.100116","url":null,"abstract":"<div><div>The domestic cat is an example of a species that thrives despite numerous threats faced by the Felidae family. This resilience has sparked significant scientific interest in the application of assisted reproductive techniques (ARTs) developed for domestic cats in order to preserve the genetic diversity of wild felines. With the decline in genetic diversity and population numbers of wild cats, effective reproductive interventions are crucial. The recovery of spermatozoa from the epididymis is important for the application of ARTs because of their unique characteristics, including their ability to maintain viability for long periods under refrigeration, their potential for cryopreservation, and their usefulness in generating viable embryos through ARTs. Techniques such as compression, perforation, and cutting followed by floating are used to recover these spermatozoa, which facilitates their use <em>in vitro</em> fertilization and artificial insemination, while gamete cryopreservation has already been successfully implemented in domestic cats, offering a possible solution to ensure the future of wild cats. This review focuses on the unique characteristics of sperm recovered from the epididymal tail of cats, and aims to capitalize on the knowledge gained from studies on domestic cats to ensure the survival of endangered felines.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142653257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal morphology of the female urogenital apparatus in Dasyprocta fuliginosa","authors":"Elisa Carla Barra Freire ,&nbsp;Diego Ferreira de Sousa ,&nbsp;Érika Branco ,&nbsp;Elane Guerreiro Giese ,&nbsp;Frederico Ozanan Barros Monteiro ,&nbsp;Pedro Mayor ,&nbsp;Ana Rita de Lima","doi":"10.1016/j.therwi.2024.100106","DOIUrl":"10.1016/j.therwi.2024.100106","url":null,"abstract":"<div><p><em>Dasyprocta fuliginosa</em> had a gestational period of approximately 104 days, to date there are few descriptions of fetal development in this species. From animals collected through subsistence hunting in the Amazon, it was possible to follow the fetal development of the female urogenital apparatus of animals from 56 days until the end of gestation. The kidneys of younger fetuses showed discreet lobations, and from the 75th gestational day onwards they were defined as smooth unilobed organs. The glomerular composition of the cortical region of the youngest fetuses had morphological differences, with poorly developed and undifferentiated capillaries. This composition was transmuted around the 82nd gestational day. Throughout the fetal development process the ureters inserted dorsolaterally to the urinary bladder, communicating with the fetal umbilical funiculus through the urachus, and with the urethra. The same occurred with the reproductive system, which was composed of a double-type uterus, with two uterine horns, a septum and two individual cervical canals, in addition to two uterine tubes, two ovaries, a vagina with a semi-open vaginal membrane, and a vulva, which made up the external genitalia. This constitution reveals that <em>D. fuliginosa</em> during the fetal phase has a well-developed morphology of the reproductive and urinary systems.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000370/pdfft?md5=3a8d4c32b95ab895ae8121e25625e652&pid=1-s2.0-S2773093X24000370-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142021526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}