Theriogenology wild最新文献

First successful cervical artificial insemination in the European roe deer (Capreolus capreolus) 欧洲狍（Capreolus Capreolus）首次成功进行宫颈人工授精

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100136

Sara Elsafadi , Reto Giacometti , Sandra Milena Bernal-Ulloa , Susanne Holtze , Sylvia Ortmann , Davide Bosi , Mara D. Saenz-de-Juano , Alexander S. Leonard , Hubert Pausch , Frank Göritz , Thomas Hildebrandt , Susanne E. Ulbrich

{"title":"First successful cervical artificial insemination in the European roe deer (Capreolus capreolus)","authors":"Sara Elsafadi , Reto Giacometti , Sandra Milena Bernal-Ulloa , Susanne Holtze , Sylvia Ortmann , Davide Bosi , Mara D. Saenz-de-Juano , Alexander S. Leonard , Hubert Pausch , Frank Göritz , Thomas Hildebrandt , Susanne E. Ulbrich","doi":"10.1016/j.therwi.2025.100136","DOIUrl":"10.1016/j.therwi.2025.100136","url":null,"abstract":"<div><div>The European roe deer (<em>Capreolus capreolus</em>) is unique among artiodactyls for undergoing embryonic diapause, a 4–5 months period of reduced embryonic development at the blastocyst stage. However, our knowledge of the reproductive physiology and endocrinology of the seasonal roe deer, particularly regarding embryo development and diapause, remains limited. Addressing this knowledge gap is crucial and necessitates further advancements in reproductive technologies. Here, this case report documents the first successful artificial insemination resulting in a pregnancy and live birth in this species, marking a significant breakthrough in its reproductive management. The timing of artificial insemination was assessed through careful behavioral observations of the time of mating by a vasectomized teaser buck. Cervical insemination was then performed using frozen-thawed sperm collected earlier from a fertile buck. DNA sequencing of the progeny, the parents and non-related individuals in the herd confirmed the parent-offspring relationship between the fawn and the sperm donor buck. While this study serves as a proof of concept, it also identifies potential areas for enhancement, such as improving estrus detection methods, sperm handling techniques, and the insemination procedure itself. This research paves the way for advanced reproductive strategies in roe deer and potentially other species with similar reproductive characteristics.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating alternatives to flow cytometry for sex-sorting rhinoceros sperm 评估用流式细胞术对犀牛精子进行性别分类的替代方法

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100135

Louisa A. Rispoli , Elizabeth Donelan , Parker M. Pennington , Priscilla H. Joyner , Terri L. Roth

{"title":"Evaluating alternatives to flow cytometry for sex-sorting rhinoceros sperm","authors":"Louisa A. Rispoli , Elizabeth Donelan , Parker M. Pennington , Priscilla H. Joyner , Terri L. Roth","doi":"10.1016/j.therwi.2025.100135","DOIUrl":"10.1016/j.therwi.2025.100135","url":null,"abstract":"<div><div>Effective sperm sex-sorting techniques could be beneficial for managing ex-situ rhinoceros populations. Fluorescence-activated cell sorting (FACS) technology has shown promise but is limited by high cost, long sorting times, and poor compatibility with frozen sperm. This study evaluated two alternative methods for sex-sorting sperm from white (<em>Ceratotherium simum</em>; n = 5) and greater one-horned (<em>Rhinoceros unicornis</em>; n = 5) rhinoceroses. Thirteen ejaculates (five fresh and eight cryopreserved) were used to assess Toll-like receptor (TLR) activation and magnetic-activated cell sorting (MACS). The TLR method selectively reduces X-bearing sperm motility via a TLR agonist, whereas MACS separates sperm based on Y-bearing sperm having a more positive surface charge than X-bearing sperm. The TLRs were confirmed to be present in rhinoceros sperm, with TLR7 primarily localized to the head region (90–95 %) and TLR8 to the flagellum (∼50 %). Activation with a TLR8 ligand reduced velocity (P = 0.001) but did not alter the distribution of X- and Y-bearing sperm in the upper and lower swim-up layers (P = 0.259). Negatively charged magnetic nanoparticles associated and pulled a portion of sperm to the tube wall when magnetic force was applied, leaving 35–80 % of sperm free in suspension, depending on the treatment conditions. However, no enrichment for X-bearing sperm was observed in the unbound fraction (P > 0.27). These results indicate that neither method physically separated X- from Y-bearing rhinoceros sperm under the tested conditions. Nonetheless, the findings provide important foundational data for refining alternative methods for sex-sorting in this species and highlight both the potential and the challenges of applying such methods.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of cryopreservation media on fertilization rate and post-thaw characteristics of tiger (Panthera tigris ssp.) spermatozoa 低温保存介质对虎精子受精率及解冻后特性的影响

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100133

Shelley E.S. Sandmaier , Jason R. Herrick

{"title":"Influence of cryopreservation media on fertilization rate and post-thaw characteristics of tiger (Panthera tigris ssp.) spermatozoa","authors":"Shelley E.S. Sandmaier , Jason R. Herrick","doi":"10.1016/j.therwi.2025.100133","DOIUrl":"10.1016/j.therwi.2025.100133","url":null,"abstract":"<div><div>There is a growing need for the development of assisted reproductive technologies to manage tiger populations under human care. Artificial insemination has been successful in tigers and other felid species but is ultimately reliant on the quality of the sperm sample. Cryobanking of tiger spermatozoa can aid in these efforts, but viability is reduced after thawing when compared to fresh samples. The current study tested new cryopreservation media with the goal of improving post-thaw viability and motility of tiger spermatozoa. Semen was collected by electroejaculation from 13 tigers and one or more treatments (TEST egg yolk buffer, soy, soy + glutamine, and soy + trehalose) were chosen for cryopreservation of each sample. Motility, acrosome integrity, and embryo development following heterologous in vitro fertilization (IVF) of domestic cat oocytes were evaluated to determine the effectiveness of each treatment. After thawing, none of the soy-based media improved upon these characteristics when compared to TEST egg yolk buffer. Despite low (average = 20 %) motility after thawing, all samples were capable of producing embryos via IVF. Results from this study indicate that both TEST and soy-based media can be used to cryopreserve tiger spermatozoa. However, there is still much room for improvement of sperm cryopreservation in this species.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endoscopic visual anatomy of urogenital tract in female Asian elephants (Elephas maximus) 雌性亚洲象泌尿生殖道内窥镜视觉解剖

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100121

Yaduraj Khadpekar , Govind S , Niraj Dahe , Jorge Soares , Nuhacet Fernández , Tenzing L. Lachenpa , Shubham Patil , Akshay Ghatare , Vaisakh Viswam , Nikorn Thongtip , Jahan Ahmed , Nirmali Sarma

{"title":"Endoscopic visual anatomy of urogenital tract in female Asian elephants (Elephas maximus)","authors":"Yaduraj Khadpekar , Govind S , Niraj Dahe , Jorge Soares , Nuhacet Fernández , Tenzing L. Lachenpa , Shubham Patil , Akshay Ghatare , Vaisakh Viswam , Nikorn Thongtip , Jahan Ahmed , Nirmali Sarma","doi":"10.1016/j.therwi.2025.100121","DOIUrl":"10.1016/j.therwi.2025.100121","url":null,"abstract":"<div><div>Populations of Asian elephants (<em>Elephas maximus</em>) have been declining in the wild, mostly due to the habitat loss and conflicts with humans. The elephants under human care thus become a very important source and opportunity to study this species, as well as for the future conservation efforts through captive breeding and conservation programs. Although a lot of work has been done on the reproductive physiology of Asian elephants, there is a limited information available on the detailed anatomy of the reproductive tract. Endoscopy offers a direct visual observation of internal organs and examination of anatomy in live animals. We examined urogenital tracts of 30 female Asian elephants under standing sedation, with the flexible endoscope. The examinations were carried out as part of urogenital tract health assessment, and treatment of infections and pathologies. The distances of different parts from the external vulva opening, were measured. Detailed description of the visual anatomy of each of the urogenital tract parts through endoscope has been provided in this study.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"6 ","pages":"Article 100121"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Faecal oestrone, rather than an androgen, is a sensitive and consistent marker of male reproductive state in equids 粪雌激素，而不是雄激素，是一个敏感和一致的标记雄性生殖状态在马科动物

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100132

A.M. Burton , J.A. Britnell , G.K. Davies , S. Shultz

{"title":"Faecal oestrone, rather than an androgen, is a sensitive and consistent marker of male reproductive state in equids","authors":"A.M. Burton , J.A. Britnell , G.K. Davies , S. Shultz","doi":"10.1016/j.therwi.2025.100132","DOIUrl":"10.1016/j.therwi.2025.100132","url":null,"abstract":"<div><div>Measuring faecal steroid hormones can provide important information on reproductive state, health, and population demographics of wild and threatened species. Androgens are indicators of male reproductive state, dominance, and can provide sex and age metadata to samples. Steroids are difficult to measure in faeces, however, due to metabolism prior to excretion and to cross-reactivity. It is therefore essential to ensure faecal assays reflect biologically meaningful changes before using them in ecological settings. We assessed the suitability of four potential faecal biomarkers of male reproductive state in three equids: Grevy’s zebra (<em>Equus grevyi)</em>, Mountain zebra (<em>Equus zebra</em>) and Carneddau Mountain ponies (<em>Equus ferus caballus</em>). We used commercial enzyme immunoassays for three androgens: androstenedione, testosterone, epiandrosterone, and an androgen derived oestrogen, oestrone, to evaluate variation between reproductively mature and immature males. Surprisingly, faecal oestrone showed the best discrimination between male reproductive classes in all species, with higher Z-scores in reproductive males compared to foals. Neither faecal testosterone, androstenedione, nor epiandrosterone could discriminate between reproductive classes. We also observed seasonal variation in Carneddau pony stallions preceding the breeding season for oestrone and androstenedione, and found high individual variation in repeated samples from Grevy’s zebra. Finally, we compared faecal oestrone between stallions and mares; non-pregnant mares had lower oestrone than stallions but there was only a significant difference between stallions and pregnant females in Grevy’s zebra. Our results suggest that faecal oestrone, rather than an androgen, is a more optimal biomarker of male reproductive state in equids. Our study highlight the importance of ensuring faecal steroid measurements are biologically and ecologically meaningful within wildlife endocrinology.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of fecal progesterone metabolites in captive and reintroduced bantengs (Bos javanicus birmanicus) in Kanchanaburi, Thailand 泰国北碧府圈养和重新引进的斑竹（Bos javanicus birmanicus）粪便孕酮代谢物研究

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100122

Weerapun Nokkaew , Apiradee Intarapak , Seree Nakbun , Nucharin Songsasen , Nikorn Thongthip

{"title":"Study of fecal progesterone metabolites in captive and reintroduced bantengs (Bos javanicus birmanicus) in Kanchanaburi, Thailand","authors":"Weerapun Nokkaew , Apiradee Intarapak , Seree Nakbun , Nucharin Songsasen , Nikorn Thongthip","doi":"10.1016/j.therwi.2025.100122","DOIUrl":"10.1016/j.therwi.2025.100122","url":null,"abstract":"<div><div>Understanding species’ reproductive biology is important for animal propagation, and the non-invasive technique of measuring fecal progesterone is a useful method for assessing reproductive health in wildlife. In this study, ovarian cycles of the captive bantengs and reproductive health of the reintroduced individuals were evaluated by assessing fecal hormone metabolites. A year-round examination of the ovarian cycles of four female bantengs (<em>Bos javanicus birmanicus</em>) housed <em>ex situ</em> was conducted by fecal progesterone metabolites detection. Furthermore, the means of fecal progesterone level were compared between females housed under human care and reintroduced individuals. Results revealed year-round estrous cycles in <em>ex-situ</em> females, unless interrupted by pregnancy. Average fecal progesterone metabolites (mean ± standard error of mean [SEM]) during the luteal phase of the four females (F1, F3, F4 and F8) were 1227.42 ± 167.45, 385.80 ± 46.55, 632.59 ± 44.19, and 872.4 ± 136.8 ng/g of dry feces, with average estrous cycle intervals of 21.14 ± 1.17 days. Mean baseline concentrations of fecal progesterone metabolites were 594.35 ± 422.59, 93.12 ± 51.71, 216.90 ± 64.92 and 166.17 ± 113.45 ng/g of dry feces, respectively. Mean fecal progesterone levels were approximately 4-fold greater (<em>P</em> < 0.001) during pregnancy than the non-pregnant period (pregnancy: 2399.35 ± 317.27 ng/g; non-pregnancy: 675.31 ± 107.8 ng/g). Mean progesterone concentration was higher (<em>P</em> = 0.028) in reintroduced females than in <em>ex-situ</em> individuals. This study advances the understanding about banteng reproductive biology, information that is critical for assessing reproductive health of both captive and reintroduced populations.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"6 ","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Follicular fluid supports cumulus expansion in post-mortem recovered cumulus-oocyte complexes of Hippopotamus amphibius 卵泡液支持死后恢复的两栖河马积云卵母细胞复合体的积云扩张

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100125

Judith Diaz-Muñoz , Sonia Gago , Paula Serres-Corral , Bruna Oliveras-Seguí , Vanessa Almagro , Teresa Mogas , Manel López-Béjar

{"title":"Follicular fluid supports cumulus expansion in post-mortem recovered cumulus-oocyte complexes of Hippopotamus amphibius","authors":"Judith Diaz-Muñoz , Sonia Gago , Paula Serres-Corral , Bruna Oliveras-Seguí , Vanessa Almagro , Teresa Mogas , Manel López-Béjar","doi":"10.1016/j.therwi.2025.100125","DOIUrl":"10.1016/j.therwi.2025.100125","url":null,"abstract":"<div><div>Assisted reproduction is essential for the conservation of wildlife species. While obtaining oocytes from living individuals can be challenging, the retrieval of post-mortem ovaries provides a valuable alternative for developing reproductive protocols that could enhance preservation efforts. In this study, we aimed, to evaluate the impact of follicular fluid (FF) supplementation during <em>in vitro</em> maturation (IVM) on nuclear maturation and cumulus expansion of common hippo (<em>Hippopotamus amphibius</em>) oocytes<em>.</em> Ovaries were recovered during the post-mortem examination of a 33-year-old female hippo, and 44 oocytes were collected and randomly allocated into three groups for IVM. A control group matured in standard media, a Small-FF group with control standard supplemented with 10 % follicular fluid from small follicles, and a Large-FF group with standard media supplemented with 10 % follicular fluid from large follicles. Images were taken at 0, 16, and 24 h of IVM to evaluate cumulus expansion by measuring the diameter of each cumulus-oocyte complex (COC). Following 24 h of IVM, polar body (PB) extrusion was assessed. Results showed a significant increase in cumulus expansion in the FF-supplemented groups compared to the Control (16 h: Control: 13 ± 4 %, Small-FF: 43 ± 13 %, Large-FF: 44 ± 6 %; 24 h: Control: 27 ± 7 %, Small-FF: 71 ± 15 %, Large-FF: 66 ± 9 %). The Small-FF group (46.1 %) and Control group (40 %) exhibited similar percentages of PB extrusion, while PB could be observed in 6.3 % of the Large-FF group. These findings suggest that while FF supplementation promotes cumulus expansion, the size of the follicle from which the fluid is derived may significantly influence oocyte ability to achieve PB extrusion</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"6 ","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High sulfate concentration disturbs the sperm motility and fertilization of salmonids 高浓度的硫酸盐会干扰鲑鱼的精子活力和受精

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100131

Mikko Mäkinen , Juha Karjalainen

{"title":"High sulfate concentration disturbs the sperm motility and fertilization of salmonids","authors":"Mikko Mäkinen , Juha Karjalainen","doi":"10.1016/j.therwi.2025.100131","DOIUrl":"10.1016/j.therwi.2025.100131","url":null,"abstract":"<div><div>Elevated sulfate (<span><math><msubsup><mrow><mtext>SO</mtext></mrow><mrow><mn>4</mn></mrow><mrow><mn>2</mn><mo>−</mo></mrow></msubsup></math></span>) effluents contribute to freshwater salinization, posing potential risks to aquatic organisms. This study aimed to evaluate the effects of increased sulfate concentrations on sperm motility and fertilization success in salmonids. The species examined included European whitefish (<em>Coregonus lavaretus</em>) and vendace (<em>Coregonus albula</em>) from freshwater and brackish environments, as well as freshwater brown trout (<em>Salmo trutta</em>). Experiments were conducted using natural water from each species' native habitat as a control and as a base for sulfate treatments, with concentrations ranging from 5–2400 mg/L in freshwater and 240–4400 mg/L in brackish water. Sperm motility was assessed at 10-, 20-, and 40-seconds post-activation using a CASA system. Increasing sulfate concentrations resulted in changes in sperm motility parameters and fertilization rate. In freshwater fish, significant difference in whitefish sperm motility compared to control treatment was observed at 450 mg/L, while in vendace, the significant differences in sperm motility and fertilization rate were observed at 1200 mg/L and 450 mg/L, respectively. For comparison, the lowest exposure concentrations where the sperm motility and fertilization rate of brackish water vendace differed from the control treatment were 1800 mg/L and 2000 mg/L, respectively. In anadromous whitefish, the significant difference in sperm motility was observed in 1200 mg/L. These findings suggest that increased sulfate levels may impair reproductive success of salmonids, underscoring the ecological risks of sulfate pollution in aquatic environments.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laparoscopic ovum pick up in common marmoset (Callithrix jacchus) 腹腔镜下普通狨猴取卵术

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100127

Heejong Eom , So-Min Lee , Dongwook Kim , Dong-Won Seol , Minkyu Kim , Dohyun Lee

{"title":"Laparoscopic ovum pick up in common marmoset (Callithrix jacchus)","authors":"Heejong Eom , So-Min Lee , Dongwook Kim , Dong-Won Seol , Minkyu Kim , Dohyun Lee","doi":"10.1016/j.therwi.2025.100127","DOIUrl":"10.1016/j.therwi.2025.100127","url":null,"abstract":"<div><div>Ovum pick up (OPU) is a crucial assisted reproductive technique for transgenic research of non-human primates, and has commonly been performed via laparotomy in common marmosets (<em>Callithrix jacchus</em>). Laparoscopy is minimally invasive and requiring smaller incisions compared to laparotomy. Four donor female (each 2 animals for laparoscopy and laparotomy) and one male (sperm collection) adult common marmosets were used in this study. Ovarian cycles were controlled and monitored for determining optimal time for OPU. Oocytes were obtained using a modified small laparoscopic system for small animals and human pediatric patients. Obtained oocytes were <em>in vitro</em> matured, fertilized and developed to blastocyst. The modified laparoscopic system provided the effective visualization of ovarian surface for OPU, and showed comparable rates of retrieval (78.57 % vs. 75.00 %), maturation (72.73 % vs. 85.19 %), fertilization (100.00 % vs. 91.30 %), and blastocyst development (63.50 % vs. 23.81 %) for oocytes between laparoscopy and laparotomy. Duration of operation, recovery time and post-operation treatment period were similar for both methods. There were no significant differences between two methods for any of the variables analyzed. In this study, the laparoscopic OPU in marmosets can achieve comparable results to laparotomy in terms of visualization, oocyte retrieval, and operation procedure. Furthermore, studies with larger sample sizes are needed to evaluate the competency of oocytes for development, along with improvements in grasping methods to enhance accessibility to ovarian surfaces in laparoscopic OPU.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100127"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the occurrence of inclusion cysts in the uterine serosa of capybaras (Hydrochoerus hydrochaeris) 水腹蛇（Hydrochoerus hydrochaeris）子宫浆膜包涵性囊肿发生的评价

Theriogenology wild Pub Date : 2025-01-01 DOI: 10.1016/j.therwi.2025.100134

Mariana Passos Nunes , Alexia Gazzola Steiner , Fabiana Morse Gosson Jorge , Ivanio Teixeira Borba-Junior , Pedro Nacib Jorge-Neto , Fernanda Battistella Passos-Nunes , Cristiane Schilbach Pizzutto

{"title":"Evaluation of the occurrence of inclusion cysts in the uterine serosa of capybaras (Hydrochoerus hydrochaeris)","authors":"Mariana Passos Nunes , Alexia Gazzola Steiner , Fabiana Morse Gosson Jorge , Ivanio Teixeira Borba-Junior , Pedro Nacib Jorge-Neto , Fernanda Battistella Passos-Nunes , Cristiane Schilbach Pizzutto","doi":"10.1016/j.therwi.2025.100134","DOIUrl":"10.1016/j.therwi.2025.100134","url":null,"abstract":"<div><div>Capybaras (Hydrochoerus hydrochaeris), the largest rodents in the world, are prolific and live in structured social groups. These characteristics, combined with their role as hosts for the bacterium Rickettsia rickettsii, make them key targets in reproductive management strategies, particularly in endemic areas of Brazilian Spotted Fever (BSF). This study evaluated 140 female capybaras subjected to surgical sterilization during management campaigns. Findings include the identification of inclusion cysts in the uterine serosa, observed in 100 % of pluriparous individuals and absent in nulliparous ones. The cysts displayed histological characteristics consistent with reports in other species, consisting of a layer of cuboidal cells and an amorphous eosinophilic material. Hypotheses for their formation include lymphatic alterations, impaired venous return, and postpartum uterine contractions. The presence of cysts in the placenta and umbilical cord is unprecedented, suggesting a potential association with hormonal factors. Although benign, these cysts warrant attention to avoid misdiagnoses. It is concluded that multiparity is a determining factor in the formation of cysts in capybaras, while healthy nulliparous individuals did not present such alterations. The absence of studies on cysts in capybaras underscores the imperative need to continue investigations to refine population management and better 29 understand the clinical impacts of these findings.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"7 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0