Theriogenology wild最新文献

{"title":"Specific setup and methodology for computer-assisted sperm analysis (CASA) in evaluating elasmobranch sperm","authors":"Pedro Nacib Jorge-Neto ,&nbsp;Fabio de Moraes Francisco ,&nbsp;Mario Davi Dias Carneiro ,&nbsp;Sergio Ricardo Brito Santos ,&nbsp;Letícia Alecho Requena ,&nbsp;Sofia Dressel Ramos ,&nbsp;Matheus Felix de Goés ,&nbsp;Rafael Franco Valle ,&nbsp;Fabiana Lucia André Padilha ,&nbsp;Helen Colbachini ,&nbsp;Rafael Caprioli Gutierrez ,&nbsp;Larissa Schneider Brandão Souza ,&nbsp;Verônica Takatsuka Manoel ,&nbsp;Laura Chrispim Reisfeld ,&nbsp;Thyara Deco-Souza ,&nbsp;Roberta Ferreira Leite ,&nbsp;Cristiane Schilbach Pizzutto","doi":"10.1016/j.therwi.2024.100091","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100091","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>One-third of elasmobranch species are threatened with extinction, and the precise assessment of their sperm parameters and quality is one stage in the development of reproductive technology for animal conservation.</p></span></li></ul><ul><li><span>2.</span><span><p>Computer-Assisted Sperm Analysis (CASA) is the gold standard for sperm analysis; however, there were not one setup established for elasmobranchs.</p></span></li></ul><p></p><ul><li><span>3.</span><span><p>This study presents the description of a specific setup developed for elasmobranch semen analysis using the CASA system.</p></span></li></ul><ul><li><span>4.</span><span><p>In addition, it provides a gold-standard technique protocol for elasmobranch sperm quality assessment.</p></span></li></ul><p></p><ul><li><span>5.</span><span><p>The described setup presented an accuracy of more than 90 percent in fourteen distinct species of freshwater and marine sharks, stingrays, and rays, failing only when there were two or more bundled sperm.</p></span></li></ul></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000229/pdfft?md5=e82e59bee8065b6a7446d1f238acb25b&pid=1-s2.0-S2773093X24000229-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141090281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm collection in rhinoceros via urethral catheterization","authors":"Anneke Moresco ,&nbsp;Justine K. O’Brien ,&nbsp;Jessye Wojtusik ,&nbsp;Monica A. Stoops","doi":"10.1016/j.therwi.2024.100090","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100090","url":null,"abstract":"<div><p>Assisted reproductive technologies are playing an ever-increasing role in the management of <em>ex situ</em> rhinoceros populations. Traditionally, sperm collection in rhinos is conducted via electroejaculation (EEJ) or gamete rescue. Sperm collection via urethral catheterization (UC) is gaining popularity in wildlife reproductive sciences as it requires less specialized equipment, is faster, and less stimulatory than EEJ. This study investigated the use of UC to collect sperm from Southern white (<em>Ceratotherium simum simum;</em> n = 3) and Eastern black (<em>Diceros bicornis michaeli;</em> n = 6) rhinoceros as an alternative or complementary to collection via EEJ. UC samples (n = 14) exhibited high sperm concentration (1.6 ± 0.49 ×10⁹ sperm/mL) and low volume (0.98 ± 0.26 mL). Samples obtained via EEJ (n = 11) were of moderate sperm concentration (0.14 ± 0.05 ×10⁹ sperm/mL) and high volume (30.6 ± 7.2 mL). UC samples were processed for cryopreservation in OptiXcell extender. Motility of UC sperm decreased post-thaw (56 ± 2.4%) over pre-freeze (77.1 ± 3.2%; <em>P</em> &lt; 0.05), with longevity data consistent to prior results published for sperm collected via EEJ. These results suggest sperm quality and cryo-survival are not impacted by the collection method. Since EEJ was conducted after UC, sperm parameters could not be compared between the two methods. Results indicate that UC is a viable alternative to EEJ for sperm collection in rhinoceros.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000217/pdfft?md5=1067a9ffa4ac0ee4d8c270d00f4db441&pid=1-s2.0-S2773093X24000217-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized heterologous in vitro fertilization with Iberian ibex sperm and domestic goat oocytes","authors":"Nuria Martínez de los Reyes,&nbsp;Melissa Carvajal-Serna,&nbsp;Inés Flores-Borobia,&nbsp;Pilar Marigorta,&nbsp;Patricia Peris-Frau,&nbsp;Julián Santiago-Moreno,&nbsp;Pablo Bermejo-Álvarez,&nbsp;Priscila Ramos-Ibeas","doi":"10.1016/j.therwi.2024.100075","DOIUrl":"10.1016/j.therwi.2024.100075","url":null,"abstract":"<div><p>Assisted reproductive technologies are key to maintain genetic stocks of endangered wild species, such as the Iberian ibex (<em>Capra pyrenaica</em>). Due to the low availability of ibex ovaries, heterologous in vitro fertilization (IVF) with domestic goat (<em>Capra hircus</em>) oocytes constitutes an excellent alternative to determine the fertilization capacity of ibex sperm doses. The aim of this study was to optimize heterologous ibex-goat IVF procedures by testing two different IVF media (TALP and SOF) and to determine whether estrous sheep serum (ESS) is required for fertilization. We found that TALP medium provides optimal conditions to conduct heterologous ibex-goat IVF, yielding blastocyst rates above 50%, and that supplementation with ESS is not required. No differences were found in embryo quality between embryos fertilized in TALP, SOF alone, or SOF supplemented with 2 or 20% ESS, based on the analysis of cell lineages development at day (D) 8 and D10 of development. Optimized heterologous ibex-goat IVF, together with embryo quality assessment through lineages development analysis, constitute an excellent system to assess fertilization capacity of ibex sperm doses, and opens the possibility of performing homologous in vitro embryo production in this species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100075"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000060/pdfft?md5=4c2b24959467dc67956c5dd1d32e3db1&pid=1-s2.0-S2773093X24000060-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139637945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold dogs: Sperm freezing, artificial insemination & non-invasive monitoring tools to facilitate a hybrid conservation management approach for endangered African wild dogs","authors":"Damien Boyd Bertrand Paul Paris ,&nbsp;Pia Riddell ,&nbsp;Carolynne Joone ,&nbsp;Morné de la Rey ,&nbsp;Andre Ganswindt ,&nbsp;Monique Christina Johanna Paris","doi":"10.1016/j.therwi.2024.100073","DOIUrl":"10.1016/j.therwi.2024.100073","url":null,"abstract":"<div><p>Given the ongoing fragmentation and decline of suitable habitat coupled with recurring disease outbreaks and pack eradications, a major rethink is required to manage the long-term genetic diversity of the endangered African wild dog. Competing financial demands in developing countries mean that conservation strategies need to yield the biggest genetic gains for the smallest economic cost. As such, we propose the development of a hybrid conservation approach to the metapopulation management of African wild dogs, which augments assisted dispersal and natural breeding initiatives with genetic gains afforded by sperm banking and artificial insemination. We discuss how such an approach would address current challenges faced by the species in the context of its unique social biology and how this could integrate with existing conservation management interventions. Moreover, during a period spanning nearly 20 years, we have developed many of the supporting reproductive technologies required to maximise genetic diversity of African wild dogs, in addition to a suite of non-invasive management tools to quantify, monitor and manipulate behaviour, vocalisations, hormones for reproductive function and responses to stressors, as well as immune function to improve the social cohesion, health and reproduction of packs.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000047/pdfft?md5=f8e155922e616043e1dd1f84bcf3e083&pid=1-s2.0-S2773093X24000047-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139540629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen collection and baseline semen characteristics of the king quail (Synoicus chinesis)","authors":"Isabella Wong ,&nbsp;Robert Doneley ,&nbsp;Stephen Johnston","doi":"10.1016/j.therwi.2024.100117","DOIUrl":"10.1016/j.therwi.2024.100117","url":null,"abstract":"<div><div>Assisted breeding technology has important management implications for avian conservation and aviculture but species-specific reproductive information is lacking. This study aimed to develop a successful semen collection for the king quail (<em>Synoicus chinesis</em>) in order to establish baseline seminal characteristics and to explore the effect of flock density on seminal quality. A total of 19 quail consisting of 2 groups of 5 and 4 and 1 group of 10 were housed in 3 separate aviaries located at the University of Queensland, Gatton, Australia during Spring. Morphometrics and morphology (normal and abnormal) of the sperm cells were also described by phase contrast and differential interference microscopy. Semen was sucessfully collected by cloacal massage from 90 % of attempts with only minor evidence of faecal or urate contamination. No significant differences in seminal characteristics were found with respect to bird housing density. Mean ± SEM seminal characteristics for 103 ejaculates included volume (1.6 ± 0.02 μL), sperm concentration (8.1 ± 0.5 × 10⁹ mL^–1), progressive sperm motility (50.0 ± 0.04 %), rate of sperm motility, (2.9 ± 0.13), pH (6.7 ± 0.2), the percentage of live sperm (88.0 ± 0.01 %) and the percentage of total abnormal sperm morphology (21.0 ± 0.03 %). Cloacal massage proved to be a highly successful technique to collect king quail semen and the seminal characteristics reported here are the future basis of assessing fertility and the application of assisted breeding in the species.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fetal morphology of the female urogenital apparatus in Dasyprocta fuliginosa","authors":"Elisa Carla Barra Freire ,&nbsp;Diego Ferreira de Sousa ,&nbsp;Érika Branco ,&nbsp;Elane Guerreiro Giese ,&nbsp;Frederico Ozanan Barros Monteiro ,&nbsp;Pedro Mayor ,&nbsp;Ana Rita de Lima","doi":"10.1016/j.therwi.2024.100106","DOIUrl":"10.1016/j.therwi.2024.100106","url":null,"abstract":"<div><p><em>Dasyprocta fuliginosa</em> had a gestational period of approximately 104 days, to date there are few descriptions of fetal development in this species. From animals collected through subsistence hunting in the Amazon, it was possible to follow the fetal development of the female urogenital apparatus of animals from 56 days until the end of gestation. The kidneys of younger fetuses showed discreet lobations, and from the 75th gestational day onwards they were defined as smooth unilobed organs. The glomerular composition of the cortical region of the youngest fetuses had morphological differences, with poorly developed and undifferentiated capillaries. This composition was transmuted around the 82nd gestational day. Throughout the fetal development process the ureters inserted dorsolaterally to the urinary bladder, communicating with the fetal umbilical funiculus through the urachus, and with the urethra. The same occurred with the reproductive system, which was composed of a double-type uterus, with two uterine horns, a septum and two individual cervical canals, in addition to two uterine tubes, two ovaries, a vagina with a semi-open vaginal membrane, and a vulva, which made up the external genitalia. This constitution reveals that <em>D. fuliginosa</em> during the fetal phase has a well-developed morphology of the reproductive and urinary systems.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000370/pdfft?md5=3a8d4c32b95ab895ae8121e25625e652&pid=1-s2.0-S2773093X24000370-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142021526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epididymal sperm characteristics in Brown-throated Sloth (Bradypus variegatus Schinz, 1825)","authors":"Gustavo Oliveira Alves Pinto ,&nbsp;José Henrique Alves Nascimento e Silva ,&nbsp;Giovanna Isabella de Souza Couto ,&nbsp;Lucas Facundo Moura Tobal ,&nbsp;Fabricio Bezerra de Sá ,&nbsp;Gustavo Ferrer Carneiro ,&nbsp;Maria Madalena Pessoa Guerra ,&nbsp;Gilcifran Prestes de Andrade","doi":"10.1016/j.therwi.2024.100113","DOIUrl":"10.1016/j.therwi.2024.100113","url":null,"abstract":"<div><div>Three-toed sloths (<em>Bradypus variegatus</em>) are mammals belonging to the superorder Xenarthra and are commonly found in the tropical forests of Latin America. Few studies have investigated aspects related to the reproduction of these animals, and there is a lack of research on the characteristics of their spermatozoa. This study aimed to evaluate spermatozoa recovered from the epididymides of four animals that were euthanized at CETRAS-Tangara in Recife, Brazil. Gamete recovery was successful in only two individuals, leading to histological analyses of the gonads and epididymides. The absence of gametes, combined with histological findings and the topographical position of the gonads, confirmed that partial testicular descent is necessary for the initiation of spermatogenesis in this species. The recovered gametes exhibited low motility (18.8 %) and poor acrosomal integrity (8 %). Despite these results, this investigation provided initial insights into the spermatozoa of <em>B. variegatus</em>, which may contribute to future research aimed at studying and preserving <em>Bradypus</em> species.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epididymal spermatozoa from domestic cats in assisted reproduction biotechniques: Perspectives for wild felid applications","authors":"Silmara Leticia Gonçalves Lima ,&nbsp;Danuza Leite Leão ,&nbsp;Adriana Novaes dos Reis ,&nbsp;Josye Bianca Santos ,&nbsp;Regiane Rodrigues dos Santos ,&nbsp;Sheyla Farhayldes Souza Domingues","doi":"10.1016/j.therwi.2024.100116","DOIUrl":"10.1016/j.therwi.2024.100116","url":null,"abstract":"<div><div>The domestic cat is an example of a species that thrives despite numerous threats faced by the Felidae family. This resilience has sparked significant scientific interest in the application of assisted reproductive techniques (ARTs) developed for domestic cats in order to preserve the genetic diversity of wild felines. With the decline in genetic diversity and population numbers of wild cats, effective reproductive interventions are crucial. The recovery of spermatozoa from the epididymis is important for the application of ARTs because of their unique characteristics, including their ability to maintain viability for long periods under refrigeration, their potential for cryopreservation, and their usefulness in generating viable embryos through ARTs. Techniques such as compression, perforation, and cutting followed by floating are used to recover these spermatozoa, which facilitates their use <em>in vitro</em> fertilization and artificial insemination, while gamete cryopreservation has already been successfully implemented in domestic cats, offering a possible solution to ensure the future of wild cats. This review focuses on the unique characteristics of sperm recovered from the epididymal tail of cats, and aims to capitalize on the knowledge gained from studies on domestic cats to ensure the survival of endangered felines.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142653257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal reproduction of Megascops choliba males in Southeastern Brazil (Aves, Strigidae): An endocrine and molecular study","authors":"Heriberto Barbosa-Moyano ,&nbsp;Marcelo Demarchi Goissis ,&nbsp;Thais Rose dos Santos Hamilton ,&nbsp;Rogério Loesch Zacariotti ,&nbsp;Luís Fábio Silveira ,&nbsp;Claudio Alvarenga De Oliveira","doi":"10.1016/j.therwi.2024.100077","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100077","url":null,"abstract":"<div><p>Reproductive activity in animals is regulated by variations in plasma levels of steroid hormones, which respond to both geophysical and social-environmental cues. Changes in testosterone (T) levels play a crucial role in coordinating the morphological, physiological, and behavioral adaptations among males, orchestrating gonadal recrudescence and regression in response to seasonal shifts in environmental factors. Although these reproductive neuroendocrine processes have been extensively examined in various bird species, they remain largely unexplored in owls. The Tropical Screech Owl is widely distributed across Central and South America, and it exhibits regional variations in the timing of chick hatching. This study hypothesized that male Tropical Screech Owls (<em>Megascops choliba</em>) exhibit fluctuations in testosterone levels and corresponding seasonal expression of reproductive genes, potentially linked to reproductive activity, including the presence of semen. To investigate this seasonal reproduction, a group of seven adult male owls was monitored for 14 consecutive months under <em>ex-situ</em> conditions. These owls underwent cloacal electrostimulation and subsequent massage to collect semen, as well as blood sampling to measure serum T levels. The expression of aromatase (CYP19A1), androgen receptor (AR), and estrogen receptor (ESR1 and ESR2) genes was also assessed in gonadal and hypothalamic tissues from four reproductively active and four non-reproductive males collected <em>in-situ</em> from a wild population. Serum T levels in males were significantly higher in July and August, coinciding with the presence of sperm. No significant difference was observed in the hypothalamic tissue transcripts of reproductive versus non-reproductive males. However, non-reproductive males exhibited higher expression of AR and ESR1 genes at the gonadal level. These results confirm the reproductive seasonality of <em>M. choliba</em> males during the winter season in São Paulo State.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100077"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000084/pdfft?md5=4fa047e0554a8bcee6762ea5c9bb9edf&pid=1-s2.0-S2773093X24000084-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140062613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm proteome and sperm parameters of captive Amazon squirrel monkeys (Saimiri collinsi Osgood, 1916) during the non-fatted and fatted condition","authors":"Danuza Leite Leão ,&nbsp;Sheyla Farhayldes Souza Domingues ,&nbsp;Wlaisa Vasconcelos Sampaio ,&nbsp;Patrícia da Cunha Sousa ,&nbsp;Fábio Roger Vasconcelos ,&nbsp;Arlindo Alencar Moura ,&nbsp;Regiane Rodrigues dos Santos ,&nbsp;Irma Caroline Oskam","doi":"10.1016/j.therwi.2024.100092","DOIUrl":"10.1016/j.therwi.2024.100092","url":null,"abstract":"<div><p>The aims of this work were (1) to characterize the proteome profile of <em>Saimiri collinsi</em> (Amazon squirrel monkey) sperm, and (2) to evaluate if there are meaningful differences in sperm quality and sperm protein landscape during the non-fatted and fatted conditions of the animals. Semen of adult males (n=4) was collected by electroejaculation and diluted in ACP-118® extender. Sperm concentration, motility, vigor, plasma membrane integrity and functionality, and sperm morphology were evaluated. Sperm proteome was characterized by mass spectrometry. The non-fatted and fatted conditions were defined according to the fattening index. There were increases in seminal volume, pH, and sperm motility of <em>S. collinisi</em> during the fatted in comparison with the non-fatted condition (p &lt; 0.05). Animals in the fatted condition had greater body mass (909 ± 23.98 g) in comparison with the non-fatted condition (818.8 ± 5.97 g, p = 0.0001). There were 1161 proteins identified in the sperm of the monkeys. Thirty-seven proteins were upregulated (p &lt; 0.05) in non-fatted condition (such as coiled-coil domain-containing protein 105, protein kinase C alpha type isoform X1, pyruvate dehydrogenase protein X component – mitochondrial and heat shock 70 kDa protein 1-like) and they were involved in cellular and metabolic processes, catalytic activity and binding. Twenty-eight sperm proteins proteins were upregulated (p &lt; 0.05) in fatted condition (such as Cytochrome b-c1 complex subunit 2, mitochondrial; calcium-binding mitochondrial carrier protein SCaMC-1-like; heat shock 70 kDa protein 13, and izumo sperm-egg fusion protein 1), participating in cellular processes, metabolic processes, and localization, binding, catalytic activity, ATP-dependent activity, and transport activity. Functional annotations of sperm proteins upregulated in non-fatted condition were described as hydrogen ion transport, chaperone, cilium, flagellum, and cell projection. In in fatted conditions, such annotations were described as fertilization, spermatogenesis and transport, chaperone, and motor protein. The present study brings an unprecedent contribution to our understanding about the reproductive biology of adult <em>Saimiri collinsi.</em> It also provides support for conservation and development of assisted reproductive technologies for Neotropical primates.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000230/pdfft?md5=693e56b7b1710ae0c805288c4c0416cb&pid=1-s2.0-S2773093X24000230-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141274389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}