The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation

Tatiana Prata Menezes , Fernanda Carolina Ribeiro Dias , Nara Clara Lazaroni e Merchid , Camila Folly Batista , Camilo Jose Ramirez-Lopez , Arabela Guedes de Azevedo Viana , Renner Philipe Rodrigues Carvalho , Adrielli Ribeiro Araújo , José Lino-Neto , Gisele Lessa , Arlindo A. Moura , Mariana Machado-Neves
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Abstract

Sperm fertility in mammals depends on the complexity of several physiological events. The male gamete is produced in the testis and matured in the epididymis, which requires an adequate environment for sperm maturation and survival. This study focused on characterizing the sperm morphology of the small wild rodent Akodon cursor and describing the epididymal environment supporting sperm maturation. Eight A. cursor males were captured from a preserved area of the Atlantic Forest in Viçosa (MG, Brazil). The epididymides were processed for histological, functional, and proteomic analyses, besides sperm quality evaluation. Data were submitted to ANOVA and the post hoc Tukey's test (P < 0.05). The length of A. cursor sperm was 52.71 ± 0.79 μm (mean ± SD), with 5.12 ± 0.07 μm corresponding to the nucleus. Sperm motility and vigor were 45.6 % ± 2.9 and 2.9 ± 0.2, respectively. The duration of sperm transit time in the epididymis was 2.75 ± 0.22 days. Histomorphometrically, the distal cauda had the highest tubular and luminal diameter, whereas the epithelial height was higher in the initial segment than in the other regions. Proteomic analysis identified 56 proteins in the proximal epididymal regions of A. cursor related to crucial processes, including cellular homeostasis, flagellar elongation, and the elimination of defective sperm during epididymal transit. These findings provide valuable insights into the molecular mechanisms underlying sperm maturation in this species. In conclusion, the epididymis of A. cursor shares similarities with other rodent species but exhibits unique characteristics, such as shorter sperm transit time.
棘齿鱼(Muridae, Sigmodontinae, Akodontini)的附睾微环境:精子从产生到成熟的生命周期
哺乳动物精子的生育能力取决于几个生理事件的复杂性。雄性配子在睾丸中产生,在附睾中成熟,这需要精子成熟和生存的适当环境。本研究的重点是表征小型野生啮齿动物Akodon游标的精子形态,并描述支持精子成熟的附睾环境。在巴西维萨罗沙(MG)大西洋森林保护区捕获8只雄性游标。除精子质量评估外,还对附睾进行组织学、功能和蛋白质组学分析。数据进行方差分析和事后Tukey检验(P <; 0.05)。游标精的长度为52.71 ± 0.79 μm(平均 ± SD),细胞核长度为5.12 ± 0.07 μm。精子活力和活力分别为45.6% % ± 2.9和2.9 ± 0.2。精子在附睾中转时间为2.75 ± 0.22 d。在组织形态学上,末梢的管腔直径和管腔直径最大,而上皮高度在起始段高于其他区域。蛋白质组学分析鉴定出了56个与关键过程相关的蛋白,包括细胞稳态、鞭毛延伸和附睾运输过程中缺陷精子的消除。这些发现为该物种精子成熟的分子机制提供了有价值的见解。综上所述,游鼠的附睾与其他啮齿类动物有相似之处,但具有精子传输时间较短等独特特征。
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来源期刊
Theriogenology wild
Theriogenology wild Animal Science and Zoology, Veterinary Science
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