{"title":"High temperature during photorefractory stage attenuates photoperiodic responses during photostimulatory stage in male tree sparrows (Passer montanus)","authors":"Zothanmawii Renthlei, Montak Mongku, Subu Yatung, Ruth Lalpekhlui, Amit Kumar Trivedi","doi":"10.1016/j.therwi.2024.100100","DOIUrl":"10.1016/j.therwi.2024.100100","url":null,"abstract":"<div><p>Photoperiod is the most reliable environmental cue, though other environmental factors may affect breeding. We hypothesized that exposure to high temperatures at the photorefractory stage suppresses photoperiodic responses during the photostimulatory phase. Male adult photorefractory tree sparrows (n=10/group) were exposed to short photoperiod (SD;16 L:8D) but either high (30 ± 2 <sup>0</sup>C) or low temperature (20 ± 2 <sup>0</sup>C) for five or seven weeks and then long photoperiod (LD; 16 L:8D) for 30 days. mRNA levels of thyroid-stimulating hormone-β (<em>Tshβ</em>), type 2 deiodinase (<em>Dio2</em>), type 3 deiodinase (<em>Dio3</em>), gonadotropin-releasing hormone (<em>GnRh</em>), gonadotropin inhibitory hormone (<em>GnIh</em>), and EYA transcriptional coactivator and phosphatase 3 (<em>Eya3</em>) were measured in hypothalamic and expression levels of <em>StAR</em>, <em>ER</em>, <em>CYP17</em>, <em>CYP11B</em>, <em>FOXOL1</em>, and <em>Nr4A1</em> were measured in the testes. Results show that exposure to 5 weeks of high temperature coupled with Short days suppresses expression of <em>Tshβ</em>, <em>Dio2</em>, and <em>GnRh</em> in the hypothalamus and <em>StAR</em>, <em>ER</em>, <em>CYP17A1</em>, and <em>CYP11B</em> in the testes under long days. No such effects were observed in birds exposed to high temperatures along with SD for seven weeks. These findings suggest that exposure to high temperature during the photorefractory stage modulates photoperiodic responses during the photostimulatory stage in time dependent manner.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100100"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X2400031X/pdfft?md5=a304f076296355b7996dc9d0e3811de8&pid=1-s2.0-S2773093X2400031X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141413852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2023-12-06DOI: 10.1016/j.therwi.2023.100066
Luciana M. Melo , Sâmara B. Silva , Lívia C. Magalhães , Jenin V. Cortez , Satish Kumar , José M.B. Duarte , Luciana D. Rola , Maiana S. Chaves , Vicente J.F. Freitas
{"title":"Erratum to “The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: embryo development and nuclear gene expression” Theriogenol. Wild 1 (2022) 100001","authors":"Luciana M. Melo , Sâmara B. Silva , Lívia C. Magalhães , Jenin V. Cortez , Satish Kumar , José M.B. Duarte , Luciana D. Rola , Maiana S. Chaves , Vicente J.F. Freitas","doi":"10.1016/j.therwi.2023.100066","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100066","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100066"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X2300051X/pdfft?md5=98fe1839ca4c31fb74c5a49e44599a19&pid=1-s2.0-S2773093X2300051X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138501830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2024-06-17DOI: 10.1016/j.therwi.2024.100097
Kerry V. Fanson , Janine L. Brown , Tamara Keeley , Beth M. Roberts , Franz Schwarzenberger
{"title":"Special issue: Biomarkers of reproductive health in wildlife","authors":"Kerry V. Fanson , Janine L. Brown , Tamara Keeley , Beth M. Roberts , Franz Schwarzenberger","doi":"10.1016/j.therwi.2024.100097","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100097","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100097"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X2400028X/pdfft?md5=d73cfb7410eb3e5403e09f0b44082d08&pid=1-s2.0-S2773093X2400028X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141424392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2024-10-16DOI: 10.1016/j.therwi.2024.100114
Leonardo Carvalho , Gleide Fernandes de Avelar , Flávia Cappuccio de Resende
{"title":"Sperm storage in males of the Neotropical rattlesnake Crotalus durissus (Squamata: Viperidae): Structure and seasonal variation of the distal ductus deferens","authors":"Leonardo Carvalho , Gleide Fernandes de Avelar , Flávia Cappuccio de Resende","doi":"10.1016/j.therwi.2024.100114","DOIUrl":"10.1016/j.therwi.2024.100114","url":null,"abstract":"<div><div>In species with asynchronous reproductive cycles, where gamete production is not aligned with the mating season, either males or females must store sperm. This reproductive tactic is an obligatory feature of male rattlesnakes’ reproductive cycle due to asynchrony between spermatogenesis and mating. Given that the ductus deferens is the primary site of sperm storage in male snakes, we aimed to investigate the morphological and histochemical changes in the distal ductus deferens of <em>C. durissus</em> throughout its reproductive cycle. In this species, spermatogenesis begins in spring and peaks in summer, while testes regress during autumn and winter. The distal ductus deferens of 28 mature male specimens was evaluated using histomorphometric and histochemical methods. Spermatozoa were consistently observed within the lumen of the ductus deferens in almost all specimens. The principal cells of the distal region of ductus deferens reacted positively for Periodic Acid-Schiff and Bromophenol Blue. Secretions were observed in the apical region of the principal cells' cytoplasm and along the epithelium edge, which may be related to gamete maintenance. Increased secretory activity of the principal cells was observed during periods of testicular activity. A reduction in the lumen of ductus deferens occurs during testicular regression, indicating possible fluid resorption by epithelial cells. Fluid resorption might be one of the mechanisms to ensure stored sperm viability, as it provides an increase in the glycoprotein’s concentration.</div></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2024-05-26DOI: 10.1016/j.therwi.2024.100094
Brenda Braga , Cassiane Leal , Verônica Oliveira-Bahia , Maria Auxiliadora Ferreira , José Ribamar Felipe Marques , Diva Araújo Guimarães
{"title":"Characteristics of Kinosternon scorpioides turtle embryos through egg candling","authors":"Brenda Braga , Cassiane Leal , Verônica Oliveira-Bahia , Maria Auxiliadora Ferreira , José Ribamar Felipe Marques , Diva Araújo Guimarães","doi":"10.1016/j.therwi.2024.100094","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100094","url":null,"abstract":"<div><p>Effective management practices are critical for the successful reproduction of animals in captivity or in <em>in situ</em> environments. One of the techniques used in egg quality control is candling. In <em>ex situ</em> reproduction of <em>Kinosternon scorpioides</em>, it is important to establish incubation protocols, which, through egg management, allow control of embryonic development and improved birth rates. We incubated eggs and monitored embryonic development of <em>K. scorpioides</em> through egg candling. Fertile eggs were incubated, and candling analyses were performed weekly. Embryonic development in the early stages (first third of incubation) presented the following characteristics: presence of the vitelline circulatory system, expansion of this system, and descent of the embryo onto the yolk. Then, in the middle stages (second third of incubation), the intense pigmentation of the embryo was observed, with the body moving to prone position. Subsequently, in the late stages, the embryo grew, filling a large part of the internal space of the egg. The incubation period took on average 116.50 ± 13.11 days. Candling can be used to monitor the embryonic development of <em>K. scorpioides</em>. It is a useful tool to improve the production of hatchlings, from fertility detection to hatching.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100094"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000254/pdfft?md5=430ecd048b6e1603143a4fe0626fb625&pid=1-s2.0-S2773093X24000254-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141242255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In situ and ex situ jaguar (Panthera onca) reproduction: What do we have so far?","authors":"Thyara Deco-Souza , Gediendson Ribeiro Araújo , Cristiane Schilbach Pizzutto , Letícia Alecho Requena , Pedro Nacib Jorge-Neto","doi":"10.1016/j.therwi.2024.100070","DOIUrl":"10.1016/j.therwi.2024.100070","url":null,"abstract":"<div><p>Assisted reproductive technologies enable the transportation and exchange of genetic material, so it is an essential complementary tool in conservation programs. Unfortunately, the difficulty of accessing a significant number of animals under suitable physiological conditions hampers the advancement of these technologies. Pharmacological semen collection in jaguars is effective in acquiring a sufficient amount of sperm. However, the outcomes of artificial insemination (AI) and in vitro fertilization operations are impacted by poor sperm quality and limited freezability of the cells. Hormonal stimulation with eCG and hCG may be used to collect oocytes in jaguars; however, proximity to males influences ovarian response to exogenous hormones. Thus, the non-copulatory ovulation stimuli pattern must be considered in AI and laparoscopic ovum pick-up protocols in this species. Somatic cells may be an alternative for biobanks since they are easier to collect and transport and can be obtained from any age, sex, or origin of an animal, including post-mortem samples. Our research group recently succeeded in cultivating cells from three jaguars that were found dead on highways, demonstrating that cultivation is effective even in degenerating tissues. In this instance, xenotransplantation of cryopreserved fragments improved culture effectiveness and brought cell quality back up to par with that of fresh tissue.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100070"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000011/pdfft?md5=3243db089778a01cb518147cfda741d6&pid=1-s2.0-S2773093X24000011-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139396001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2024-01-06DOI: 10.1016/j.therwi.2024.100071
Heather M. Manitzas Hill , Malin K. Lilley , Jackson R. Ham , Todd Robeck
{"title":"A review of beluga (Delphinapterus leucas) sexual behavior and reproductive physiology leading to conception","authors":"Heather M. Manitzas Hill , Malin K. Lilley , Jackson R. Ham , Todd Robeck","doi":"10.1016/j.therwi.2024.100071","DOIUrl":"10.1016/j.therwi.2024.100071","url":null,"abstract":"<div><p>This review summarizes the available literature on the knowledge of reproduction in white whales or belugas (<em>Delphinapterus leucas</em>). We discuss reproductive anatomy, physiology, endocrinology, and behavior prior to conception. Following a description of these basic reproductive parameters for each sex, the review describes the mating system of belugas as it is currently understood and summarizes the ontogeny of reproductive behavior and its relationship with hormones. Finally, we highlight gaps that currently exist in our functional understanding of reproductive behavior in belugas. Future research emphasizes the need to understand mate choice in belugas and how it manifests across the lifespan and potentially settings. Specifically, does the quality of belugas’ display behaviors influence reproductive success? Managing wild populations of belugas and caring for belugas in human care both benefit from the knowledge that can be gained from the research topics discussed in this review.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100071"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000023/pdfft?md5=57109aada70ebab53f135b18b5df3343&pid=1-s2.0-S2773093X24000023-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139392379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Theriogenology wildPub Date : 2024-01-01Epub Date: 2024-05-06DOI: 10.1016/j.therwi.2024.100088
Raffaella Simone , Daniel Čižmár , Susanne Holtze , Baptiste Mulot , Benjamin Lamglait , Tobias Knauf-Witzens , Annika Weigold , Robert Hermes , Thomas B. Hildebrandt
{"title":"Cryopreservation of okapi (Okapia johnstoni) oocytes following in vitro maturation","authors":"Raffaella Simone , Daniel Čižmár , Susanne Holtze , Baptiste Mulot , Benjamin Lamglait , Tobias Knauf-Witzens , Annika Weigold , Robert Hermes , Thomas B. Hildebrandt","doi":"10.1016/j.therwi.2024.100088","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100088","url":null,"abstract":"<div><p>Okapi (<em>Okapia johnstoni</em>) belong to the Giraffidae family and is the only representative of the <em>Okapia</em> genus. The species has been declared as endangered and due to its very elusive nature, little is known about its reproductive behavior. The wild population has continued to decrease over the last decades despite the different conservation programs developed worldwide. Captive breeding is difficult and the European population is not self-sustaining. An alternative tool to prevent the extinction of this charismatic species is the use of assisted reproductive technologies (ART). Specifically, we introduce a novel protocol for the in vitro maturation (IVM) and cryopreservation of matured oocytes obtained post-mortem. Out of sixteen cumulus-oocytes complexes isolated from one deceased individual, four matured metaphase II (MII) oocytes were successfully obtained following IVM. Furthermore, these matured oocytes demonstrated resilience during the vitrification/warming process and underwent sperm injection. Although no embryos were obtained, this study represents the first step towards the application of ART in okapi, providing a foundation for future reproductive interventions aimed at preserving self-sustaining captive populations.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"4 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000199/pdfft?md5=d902cd07f65fdc0beeb146eea08ffc29&pid=1-s2.0-S2773093X24000199-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140924468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Exploring enzymatic antioxidant activities in Asian Elephant (Elephas maximus) sperm and seminal plasma","authors":"Yuqing Yang , Junpen Suwimonteerabutr , Taweepoke Angkawanish , Nicole Sirisopit Mehl , Patharapol Piamsomboon , Nuttawadee Ngamlertwong , Padet Tummaruk , Pakpoom Navanukraw , Phakjira Sanguansook , Kaywalee Chatdarong","doi":"10.1016/j.therwi.2024.100103","DOIUrl":"https://doi.org/10.1016/j.therwi.2024.100103","url":null,"abstract":"<div><p>This study explored the relationships between sperm quality and the activities of antioxidant enzymes (superoxide dismutase, SOD, and glutathione peroxidase, GPx) in sperm (S) and seminal plasma (SP) of Asian elephants, as well as the potential protective role of glutamine in semen extenders during chilled storage. In experiment 1, twenty ejaculates from 11 bulls were analyzed for percentages (%) of sperm motility (%MOT), viability (%VIA), and activities of SOD and GPx. A significant negative correlation was found between %VIA and SP-SOD (r = − 0.71), and positive correlations between %MOT and both S-GPx (r = 0.49) and SP-GPx (r = 0.57). Following these findings, glutamine, a precursor of glutathione and semen antioxidant was introduced in Experiment 2. Each ejaculate (in a total of 8 ejaculates obtained from four bulls) was diluted in Tris-fructose- (EYT) and Tris-glucose-egg yolk extender (TCG), respectively, supplemented with 0 (control), 20 and 40 mM glutamine and stored at 4 °C. Sperm parameters including %MOT, %VIA, functional membrane integrity (%sHOST), and DNA integrity (%DNA) were assessed after 24 and 48 h of storage. Results demonstrated that glutamine did not significantly affect %VIA or %DNA. A significant reduction in %MOT was observed at 24 h, but 20 mM glutamine added in extenders reversed this decline at 48 h. A 40 mM addition of glutamine in TCG markedly reduced %sHOST at 24 h. In conclusion, SOD and GPx activities in the sperm and seminal plasma are indicative of sperm quality. During chilling, using a higher level of glutamine may damage the spermatozoa of Asian elephants.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"5 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773093X24000345/pdfft?md5=bf30ffee5ee7d7c568a5012b6da26f81&pid=1-s2.0-S2773093X24000345-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}