Theriogenology wild最新文献

{"title":"First detailed description of follicle development in a chimaeroid, the American elephant fish Callorhinchus callorhynchus","authors":"S.N. Fuentes ,&nbsp;C.A. Awruch ,&nbsp;C. Moya ,&nbsp;A. Wehitt ,&nbsp;M.C.Díaz Andrade","doi":"10.1016/j.therwi.2023.100037","DOIUrl":"10.1016/j.therwi.2023.100037","url":null,"abstract":"<div><p>Chimaerods represent an enigmatic lineage of cartilaginous fishes and the least studied group within Chondrichthyans. They have a widespread global distribution with a high degree of endemism. In addition, this group has been considered among the least threatened species of Chondrichthyans, but also the most data-deficient group, with biological information lacking for most species. This study represents the first microanatomical, histological, and ultrastructural description of the different stages of folliculogenesis in <em>Callorhinchus callorhynchus</em>, and the first ultrastructural study about this topic within chimaeroids. Specimens of different maturity stages were collected from landings of the commercial fleet operating in San Matías Gulf, Argentina. Folliculogenesis shows some differences compared to other elasmobranchs. Oogonia were present in immature and mature females. Atretic follicles of different sizes were present in mature female ovaries. There is a significant difference between the macroscopic (∼10 mm) and microscopic (∼ 2 mm) follicular size at the beginning of vitellogenesis. There is no evidence of an epigonal organ. The results are discussed in a physiological and reproductive context. This study provides valuable information that will contribute to the understanding of the reproductive biology of the chimaeroids species and its potential for fishery exploitation recovery.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43177668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovarian cycle, reproductive performance and breeding seasonality of Amazonian yellow-footed tortoises (Chelonoidis denticulatus) in the wild","authors":"Pedro Mayor ,&nbsp;Sara Hidalgo ,&nbsp;Hani R. El Bizri ,&nbsp;Thais Q. Morcatty","doi":"10.1016/j.therwi.2023.100022","DOIUrl":"10.1016/j.therwi.2023.100022","url":null,"abstract":"<div><p>Studies of the reproductive functional morphology of chelonians are important to understand the reproductive physiology, anatomy, and endocrinology of these species. However, data on the reproduction of this group have been difficult to obtain because of their cryptic habits and as a result the consequent inefficiency of conventional field methods in detecting individuals in the wild. In this study, we obtained reproductive organs from 347 adult female yellow-footed tortoises (<em>Chelonoidis denticulatus</em>), one of the most hunted and traded land vertebrates in South America, donated by local sellers in a wild meat market in the Peruvian Amazon. We described the species’ reproductive cycle and the influence of climatic factors on reproductive performance. Of the sampled females, 116 (33.4%) were gravid and 231 (66.6%) were non-gravid, including 215 vitellogenic (62.0%) and 16 non-vitellogenic (4.6%). The mean number of ovarian follicles per female declined from 28.5 (&lt;10 mm diameter), to 7.1 follicles (20–40 mm) to 3.2 follicles&gt; 40 mm. Gravid females had in average 3.7 shelled eggs in the oviduct, with a mean egg diameter of 53.7 mm. The estimated annual reproductive potential in gravid females was 5.1 eggs per clutch, ranging from one to 22 eggs. There was a strong positive relationship between the diameter of shelled eggs and the straight carapace length of gravid females. The final phase of the folicular growth, the most demanding energetic process in chelonian reproduction, correlated with annual rainfall peak, while oviposition was estimated to occur in the dry season. The yellow-footed tortoise should be considered an opportunistic seasonal breeder, though capable of reproducing throughout the year. Reproductive yields are linked to climatic events that influence food availability in the environment. To improve the sustainable use of this species, adult females should be harvested primarily during reproductive quiescence, from the end of the laying period in the late dry season to the mid-rainy season when large follicles appear, to avoid taking gravid females. Our results are useful not just to better understand the reproductive biology of terrestrial chelonians but can inform the conservation management of harvested species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100022"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43571267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of hormones in assessing reproductive physiology of humpback whales (Megaptera novaeangliae) from Juneau, Alaska","authors":"S. Atkinson ,&nbsp;V. Melica ,&nbsp;S. Teerlink ,&nbsp;K. Mashburn ,&nbsp;J. Moran ,&nbsp;H. Pearson","doi":"10.1016/j.therwi.2023.100050","DOIUrl":"10.1016/j.therwi.2023.100050","url":null,"abstract":"<div><p>Humpback whales (<em>Megaptera novaeangliae</em>) in Southeast Alaska have been studied for over 50 years, and are largely considered a recovery success since the cessation of commercial whaling. Reproductive physiology is an important factor to consider in studying population health and can provide important insights into the drivers contributing to population abundance fluctuations. Validated assays for progesterone and testosterone were used on blubber biopsies from humpback whales (N = 33 whales, 71 samples) near Juneau, Alaska, in 2020 and 2021. Long-term sighting histories were used to confirm detected pregnancies with calf sightings the following year. Blubber samples were divided into two seasonal bins (early and late summer). Pregnant females sampled in both early and late summer of both 2020 and 2021 showed elevated progesterone concentrations compared to other reproductive states (p &lt; 0.05). Progesterone concentrations in adult male whales (0.3 ± 0.2 ng/g) were not significantly different from lactating or resting female whales. Blubber testosterone concentrations in adult male humpback whales ranged from 0.05 to 1.1 ng/g, and mean concentrations were approximately double those of female whales in any reproductive state. Pregnancy was detected in 5 of 11 and 4 of 9 adult females in 2020 and 2021 respectively, yielding summer season pregnancy rates for sexually mature females at 0.45, and 0.44, respectively. Calving rates were 0.36 and 0.22 in 2020 and 2021, respectively, and the annual growth rate for this subpopulation was calculated at 2.6 % per annum. One female had successful pregnancies for four consecutive years. These results demonstrate the synergistic value of combining immunoreactive assays and long-term sighting histories to further knowledge of reproductive physiology in individual humpback whales, which can be expanded to assessing the health of a population or ecosystem.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42341793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen cryopreservation in Bornean Sun Bear (Helarctos malayanus euryspilus)","authors":"Boon Nie Yeoh ,&nbsp;Zainal Zahari Zainuddin ,&nbsp;Mark Wen Han Hiew ,&nbsp;Siti Aisyah Sidik ,&nbsp;Siew Te Wong ,&nbsp;Symphorosa Sipangkui ,&nbsp;Abdul Hamid Ahmad","doi":"10.1016/j.therwi.2023.100057","DOIUrl":"10.1016/j.therwi.2023.100057","url":null,"abstract":"<div><p>Semen cryopreservation is one common tool for extended genome storage and serves as insurance for declining biodiversity. In the endangered Bornean sun bear, semen was collected with electroejaculation and this is the first attempt at semen cryopreservation for this subspecies. Chilled semen with motility of more than 50 % was extended with Caniplus freezing medium, and cryopreserved with slow freezing protocol. A total of 22 cryopreserved semen straws were evaluated with computer-assisted sperm analysis and conventional semen evaluation tests as well as additional functional tests including evaluation of the acrosome (Rose Bengal Fast Green stain), plasma membrane (hypoosmotic swelling test), and chromatin (toluidine blue stain). Post-thaw semen quality was compromised with poor viability (27.6 ± 11.2 %), motility (8.3 ± 7.1 %), and progressive movement (1.3 ± 2.0 %) but maintained high integrity for the acrosome (71.1 ± 10.8 %), plasma membrane (54.7 ± 9.6 %), and chromatin (89.7 ± 7.6 %). The total post-thaw sperm abnormality was 51.7 ± 10.7 %, predominantly bent tail (28.0 ± 7.6 %) and proximal droplets (20.0 ± 11.8 %). Although this current cryopreservation was not a success, the compromised frozen-thawed semen may be a valuable resource in assisted reproductive technologies such as intracytoplasmic sperm injection. The three additional functional tests demonstrated were simple and affordable and hence are recommended to be part of the routine semen evaluation. Further research is required to develop species-specific cryopreservation protocols and to explore other assisted reproductive technologies in the Bornean sun bear.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46743382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolactin enzyme-linked immunosorbent assay for rhinoceroses – Another tool for assessing reproductive function and dysfunction in this taxon","authors":"Terri L. Roth ,&nbsp;Elizabeth M. Donelan ,&nbsp;Louisa A. Rispoli ,&nbsp;T. Reilly","doi":"10.1016/j.therwi.2023.100035","DOIUrl":"10.1016/j.therwi.2023.100035","url":null,"abstract":"<div><p>For decades, progesterone and its metabolites have served as the primary biomarkers for monitoring reproductive activity in rhinoceroses, whereas protein hormones have received little attention despite their potential value in understanding reproductive function and dysfunction in this taxon. The goals of this study were to: 1) identify an enzyme-linked immunosorbent assay (ELISA) effective in measuring rhinoceros serum prolactin, 2) generate representative prolactin data in male and female rhinoceroses in diverse reproductive states, and 3) characterize prolactin throughout pregnancy in a white rhinoceros that exhibited mid-gestation lactation. Our results indicated that an equine prolactin ELISA by CUSABIO® is effective in measuring serum prolactin concentrations in white, black and Sumatran rhinoceroses. Preliminary data suggested that prolactin is lowest in males and acyclic females, but also appeared low during post-partum lactation. In contrast, prolactin concentrations were elevated in pregnant females and moderate in sexually mature females experiencing reproductive cyclicity. Prolactin increased following conception and generally continued to rise throughout pregnancy in the one pregnant white rhinoceros profiled herein. Spikes and dips in prolactin and progesterone, respectively, were documented around the time of mid-gestation mammary development and colostrum production in this individual and may provide some clues into the physiological triggers of this newly described aberrant reproductive condition. In conclusion, we have identified a new tool for studying reproductive activity in rhinoceroses, generated preliminary data, and revealed an intriguing relationship between prolactin fluctuations and premature lactation. This work provides the foundation for larger, focused studies on the role of prolactin in this taxon.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42116562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological semen collection in giant anteaters (Myrmecophaga tridactyla): A feasible option for captive and free-living animals","authors":"Gediendson Ribeiro de Araújo ,&nbsp;Pedro Nacib Jorge-Neto ,&nbsp;Jorge Aparecido Salmão-Júnior ,&nbsp;Maitê Cardoso Coelho da Silva ,&nbsp;Ricardo Zanella ,&nbsp;Antonio Carlos Csermak-Júnior ,&nbsp;Fabio de Moraes Francisco ,&nbsp;Thyara de Deco-Souza ,&nbsp;Cristiane Schilbach Pizzutto","doi":"10.1016/j.therwi.2023.100030","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100030","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49775826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a diluent storage media and effect of cryoprotectants on semen collected from white spotted bamboo sharks (Chiloscyllium plagiosum) and cooled to 5 ºC","authors":"Gisele A. Montano ,&nbsp;Karen J. Steinman ,&nbsp;Amanda McDonnell ,&nbsp;Todd R. Robeck","doi":"10.1016/j.therwi.2023.100017","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100017","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49775842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal behavioural analysis during a successful captive breeding of jaguars Panthera onca","authors":"Pamela Zaganin Modena ,&nbsp;Cristina Harumi Adania ,&nbsp;Vinicius Marques Lopez ,&nbsp;Rhainer Guillermo-Ferreira","doi":"10.1016/j.therwi.2023.100027","DOIUrl":"10.1016/j.therwi.2023.100027","url":null,"abstract":"<div><p>The breeding of captive wild species can play a crucial role in their preservation. Jaguars, the largest felines in the Americas, face challenges in captivity that hinder their breeding success in zoos and conservation institutions. One major barrier to successful captive breeding is the difficulty of replicating the natural environment, including nutritional, social, and habitat needs. Hence, any insights into the breeding behaviour and parental care of jaguars can inform and enhance breeding strategies for these captive felines. In this study, we aimed to examine maternal behaviours during the pre- and post-partum periods in the first successful case of natural reproduction of captive jaguars in Brazil. The study took place at the Mata Ciliar Association in Jundiaí , Brazil, where infrared cameras were used to observe maternal behaviours without human interference. The behaviours were analysed using an ethogram of the mother and Generalized Linear Models (GLM) to examine her behavioural repertoire during three different phases of parental care. Our findings suggest that there were differences in the behaviours exhibited by the female jaguar in the pre- and post-partum periods. Furthermore, the mother's interactions with her jaguar cubs reduced her stereotyped behaviours, implying an improvement in her welfare. Finally, we offer suggestions and recommendations to facilitate future breeding efforts for captive jaguars.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"2 ","pages":"Article 100027"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46674055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient recovery of in vivo mature and immature oocytes from jaguars (Panthera onca) and pumas (Puma concolor) by Laparoscopic Ovum Pick-Up (LOPU)","authors":"Pedro Nacib Jorge-Neto ,&nbsp;Letícia Alecho Requena ,&nbsp;Gediendson Ribeiro de Araújo ,&nbsp;Anneliese de Souza Traldi ,&nbsp;Thiago Cavalheri Luczinski ,&nbsp;Thyara de Deco-Souza ,&nbsp;Cristiane Schilbach Pizzutto ,&nbsp;Hernan Baldassarre","doi":"10.1016/j.therwi.2023.100042","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100042","url":null,"abstract":"","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49709113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into southern white rhinoceros (Ceratotherium simum simum) reproduction: Revealing granulosa cell gene expression","authors":"Elena Ruggeri ,&nbsp;Kristin Klohonatz ,&nbsp;Marc-André Sirard ,&nbsp;Barbara Durrant ,&nbsp;Stephen Coleman","doi":"10.1016/j.therwi.2023.100055","DOIUrl":"https://doi.org/10.1016/j.therwi.2023.100055","url":null,"abstract":"<div><p>In vivo-collected granulosa cells (GC) from the southern white rhinoceros (SWR) provide a non-invasive assessment of the developmental status of oocytes prior to in vitro culture, which could aid in the development of assisted reproductive technologies (ART). Our study aimed to investigate gene expression in SWR granulosa cells, collected in vivo and gain preliminary insight into the transcriptional activity occurring within the cells during various stages of oocyte development. It was hypothesized there would be similarities between the SWR GC transcriptome and cattle and humans, two species for which well-annotated genomes are available and ART are commonly used. GC were collected from SWR following ovum pickup (OPU) and pooled from all aspirated follicles. Total RNA was isolated, libraries prepared, and sequencing performed using an Illumina NextSeq 500. Reads were aligned and annotated to CerSimCot1.0. Databases for cattle and human were acquired for comparison. This study identified 37,407 transcripts present in GC of SWR. It was determined that cattle and human transcriptomes are valuable resources with a homology of 45 % with the SWR. In conclusion, these data provide preliminary, novel insights into the transcriptional activity of GC in the SWR that can be used to enhance ART in this species.</p></div>","PeriodicalId":75220,"journal":{"name":"Theriogenology wild","volume":"3 ","pages":"Article 100055"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49709114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}