The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)最新文献_第2页

Impact of Nanofiller Fractions on Selected Properties of Microfilled Composite Resin. 纳米填料组分对微填充复合树脂选择性能的影响。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-06-11 DOI: 10.3290/j.cjdr.b6260583

Enni Parpo, Lippo Lassila, Pekka K Vallittu, Sufyan Garoushi

{"title":"Impact of Nanofiller Fractions on Selected Properties of Microfilled Composite Resin.","authors":"Enni Parpo, Lippo Lassila, Pekka K Vallittu, Sufyan Garoushi","doi":"10.3290/j.cjdr.b6260583","DOIUrl":"https://doi.org/10.3290/j.cjdr.b6260583","url":null,"abstract":"Objective: To assess the impact of incorporating various weight fractions of nanometre-sized particulate fillers on specific properties of microfilled composite resin.Methods: Microfilled composite resin was prepared by mixing 29 wt.% of resin matrix (BisGMA/TEGDMA) with the 71 wt.% of silane treated particulate fillers (Ø 0.4 µm). Then, various fractions of nanometre-sized (180 nm) fillers (0, 5, 10, 15, 20, 25, 30 and 35 wt.%) were added gradually using a high-speed mixing machine. For each composite resin, flexural properties (n = 8) were evaluated using a three-point bending test on a universal testing machine (ISO standard 4049). Fourier transform infrared (FTIR)-spectrometry was used to calculate the degree of monomer conversion (DC%). Surface microhardess (Vickers) was also determined. Surface gloss was measured before and after polishing (4,000-grit paper). A two-body wear test was performed in a ball-on-flat configuration using a chewing simulator with 15,000 cycles. A non-contact 3D optical profilometer was utilised to measure wear depth. An analysis of variance (ANOVA) was applied to interpret the results statistically, then a post hoc Tukey analysis was performed.Results: ANOVA revealed that the fraction of nanofillers had a significant effect (P 0.05) on flexural modulus, DC%, microhardness, gloss and wear depth. The group without nanofillers showed the highest DC% (56.6%), gloss after polishing (76.2 GU) and wear resistance (24.2 µm) values, whereas the group with 35 wt.% of nanofillers had the highest flexural modulus (9 GPa) and microhardness (70 VH).Conclusion: It is beneficial to add nanofillers to microfilled composite resin; however, it is essential to assess the proportion ratio carefully. Optimising all the properties of composite resin at once with just one formulation is challenging.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 2","pages":"115-122"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lineage Tracing Identified Cell Populations within Adult Stem Cell Niches for Oral Maxillofacial Hard Tissue Formation. 谱系追踪鉴定了口腔颌面部硬组织形成的成体干细胞壁龛内的细胞群。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-06-11 DOI: 10.3290/j.cjdr.b6260563

Shuang Yang, Chang Hao Yu, Fei Fei Li, Yu Shi, Hui Wang, Wei Dong Tian, Quan Yuan, Ling Ye, Fan Yuan Yu

{"title":"Lineage Tracing Identified Cell Populations within Adult Stem Cell Niches for Oral Maxillofacial Hard Tissue Formation.","authors":"Shuang Yang, Chang Hao Yu, Fei Fei Li, Yu Shi, Hui Wang, Wei Dong Tian, Quan Yuan, Ling Ye, Fan Yuan Yu","doi":"10.3290/j.cjdr.b6260563","DOIUrl":"https://doi.org/10.3290/j.cjdr.b6260563","url":null,"abstract":"The regeneration of oral maxillofacial hard tissues is currently one of the issues of most concern in public health. This complex process involves a variety of cell types residing in a specialised microenvironment known as the adult stem cell niche in living organisms. Within this niche, adult stem cells are considered to play a central role in the regeneration of hard tissues, which undergo rapid proliferation and differentiation into progenitor cells to replace lost tissue, throughout postnatal life. Their fate is tightly regulated by the niche factors secreted by the non-stem niche cells present within the same microenvironment. Over the past decades, the advent of lineage tracing techniques has revolutionised the in vivo study of cell dynamics. Through tissue- and temporally-specific labelling of Cre-expressing cells, this method enables researchers to depict the defined cell fates and differentiation trajectories. The present review summarises the progress made in lineage tracing studies of hard tissue formation cell populations residing in the oral and maxillofacial regions, with a focus on stem cells, progenitor cells and niche cells. The aim is to provide new clues for future research endeavours.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 2","pages":"89-104"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the Canalis Sinuosus Using CBCT in Pathological Lesions. CBCT对鼻窦管病理病变的评价。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-06-11 DOI: 10.3290/j.cjdr.b6260624

Numan Dedeoğlu, Oğuzhan Altun

{"title":"Assessment of the Canalis Sinuosus Using CBCT in Pathological Lesions.","authors":"Numan Dedeoğlu, Oğuzhan Altun","doi":"10.3290/j.cjdr.b6260624","DOIUrl":"https://doi.org/10.3290/j.cjdr.b6260624","url":null,"abstract":"Objective: To assess the canalis sinuosus (CS) in pathological lesions located in the anterior maxilla using CBCT.Methods: In total, 104 lesions in the anterior maxilla were assessed. The localisation of CS termination points on the alveolar crest was evaluated. Subsequently, the consistency of the CS and CS-lesion relationships were determined based on the maximal diameter of the lesion and the presence of a cortical perforation.Results: Of the 104 lesions, 82 (78.8%) exhibited at least one CS. The presence of CS was statistically significantly different based on the diameter of the lesion (P 0.001). When the presence of CS was investigated in relation to cortical perforation status, a significant difference was observed (P 0.05). Anatomically, CS was most common in the central incisor and ended most frequently near the crest apex vertically and in the palatal region horizontally. Among the instances of CS, 55.3% were lesion-related, 22.3% were in contact and 23 (22.3%) were unrelated to the lesion.Conclusion: The incidence of CS was high in anterior maxillary pathological lesions and even higher in small-sized pathological lesions. Most CSs were located within or next to the surgical margin of the pathological lesion.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 2","pages":"131-137"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mandibular Osteonecrosis Involving Tooth Germ in Children: a Rare Case Report with a 6-year Follow-up. 儿童下颌骨坏死累及牙胚：一罕见病例，随访6年。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-06-11 DOI: 10.3290/j.cjdr.b6260631

Yue Fei, Guang Yun Lai, Jun Wang

{"title":"Mandibular Osteonecrosis Involving Tooth Germ in Children: a Rare Case Report with a 6-year Follow-up.","authors":"Yue Fei, Guang Yun Lai, Jun Wang","doi":"10.3290/j.cjdr.b6260631","DOIUrl":"https://doi.org/10.3290/j.cjdr.b6260631","url":null,"abstract":"Osteonecrosis involving the permanent tooth germ in primary dentition is a rare condition that can affect dental and maxillofacial development without correct intervention. This case report presents the successful recovery from drug-induced mandibular osteonecrosis involving the permanent tooth germ in a child. A healthy 4-year-old Chinese girl visited the clinic with an unhealed gingival wound and alveolar bone exposure of the missing primary molar area after 1-day arsenic trioxide sealing during pulp therapy. Radiographic examinations indicated inflammation and sequestrum formation in the jaw. The diagnosis was mandibular osteonecrosis. The treatment plans involved sequestrectomy without extended curettage and removal of the affected permanent tooth germ with systemic antibiotic therapy. A 6-year follow-up revealed no recurrence of the lesion or complications, a gradual increase in bone density of the osteonecrosis area and the development of adjacent permanent tooth germs. A conservative treatment regimen without extended curettage may be an option for young patients with jaw osteonecrosis.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 2","pages":"147-151"},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144268126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic Balance of Non-collagenous Proteins in Dentine Mineralisation. 牙本质矿化过程中非胶原蛋白的动态平衡。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097589

Min Juan Shen, Yang Yang Zhang, Meng Qi Zhu, Chun Yan Zhang, Zhi Yong Wang, Qian Ming Chen

{"title":"Dynamic Balance of Non-collagenous Proteins in Dentine Mineralisation.","authors":"Min Juan Shen, Yang Yang Zhang, Meng Qi Zhu, Chun Yan Zhang, Zhi Yong Wang, Qian Ming Chen","doi":"10.3290/j.cjdr.b6097589","DOIUrl":"10.3290/j.cjdr.b6097589","url":null,"abstract":"Dentine, the predominant structural element of the tooth, exhibits varying structural components, properties and mineralisation patterns across different regions. During dentinogenesis, diverse non-collagenous proteins (NCPs) play essential and varied roles in the mineralisation process. This paper systematically reviews the spatial distribution of NCPs across different dentine substructures and highlights their multifarious functions and collaborative interplay in governing the intricate mineralisation process. Specifically focusing on phosphorylated and glycosylated proteins, this review underscores their precisely programmed dynamic balance in orchestrating a series of distinct morphological patterns of dentinal substructures with varying degrees of mineralisation. By discussing the collaboration and balance of NCPs in dentine mineralisation, this paper also aims to advance the understanding of biomineralisation and provide valuable insights into developing highly biomimetic remineralisation strategies for dental applications.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"9-18"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eruption Status and Caries Condition of the First Permanent Molars in Chinese Children. 中国儿童第一恒磨牙萌出状况及龋病情况。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097821

Yang Yang, Xue Nan Liu, Chun Xiao Wang

{"title":"Eruption Status and Caries Condition of the First Permanent Molars in Chinese Children.","authors":"Yang Yang, Xue Nan Liu, Chun Xiao Wang","doi":"10.3290/j.cjdr.b6097821","DOIUrl":"10.3290/j.cjdr.b6097821","url":null,"abstract":"Objective: To determine whether the targeting age should be adjusted for the National Children's Pit and Fissure Sealant (PFS) Programme.Methods: Statistical analysis was conducted on the results of oral health examination results of school-aged children in regions covered by the National Children's Oral Disease Comprehensive Intervention Programme (NCODCIP) in 2018. We analysed the eruption status and dental caries condition of the children's four first permanent molars (FPMs) and performed statistical tests for the data.Results: Data analysis from 811,855 children aged 6 to 9 years showed that the complete eruption rate (CER) of the FPMs in Chinese children aged 6 years was 67.2%, and reached 94.1% by age 9. Before the implementation of the PFS Programme, the prevalence of dental caries in 6-year-olds was 11.0%, and 23.2% by age 9. Caries prevalence was higher in girls than boys. The growth rate of caries prevalence slowed with age.Conclusion: Our study indicated that the eruption time of FPMs in Chinese children has been earlier than predicted, and the caries prevalence was more severe than expected. Therefore, it is recommended that the targeting age for the National PFS Programme be lowered from 7 years old to 6 years old.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"73-78"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Biosynthetic Gene Cluster BGC3 in the Cariogenic Virulence of Streptococcus mutans. 生物合成基因簇BGC3在变形链球菌致龋毒力中的作用。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097621

Jing Yi Yang, Yi Xin Zhang, Yu Wei Zhang, Ying Chen, Min Di Xu, Dan Dan Wang, Yi Hua Chen, Yi Xiang Wang, Bin Xia

{"title":"Role of Biosynthetic Gene Cluster BGC3 in the Cariogenic Virulence of Streptococcus mutans.","authors":"Jing Yi Yang, Yi Xin Zhang, Yu Wei Zhang, Ying Chen, Min Di Xu, Dan Dan Wang, Yi Hua Chen, Yi Xiang Wang, Bin Xia","doi":"10.3290/j.cjdr.b6097621","DOIUrl":"10.3290/j.cjdr.b6097621","url":null,"abstract":"Objective: To investigate the role of the biosynthetic gene cluster BGC3 of Streptococcus mutans (S. mutans) in the process of dental caries.Methods: BGC3 and ∆BGC3 S. mutans strains were constructed and their growth curves were evaluated. Acid production capacity was assessed by evaluating pH reduction levels over identical culture periods. The survival of bacteria in phosphate citrate buffer solution (pH 3.0) was quantified. The expression levels of virulence genes (atpF, gtfC, gtfD, spaP, vicR and ftf) were analysed using the qPCR. Co-culture experiments were conducted to evaluate bacterial adaptability. Bacterial viability was determined by microscopical examination of live/dead staining.Results: Deletion of BGC3 did not significantly impact S. mutans growth or acid production in biofilms. The ∆BGC3 strain exhibited enhanced acid resistance and higher expression levels of virulence genes compared to the wild type. In addition, ∆BGC3 exhibited superior bacterial viability in the co-culture system.Conclusion: BGC3 affected the acid resistance and expression of caries-related genes in S. mutans. The BGC3 knockout strain exhibited a more robust survival capability than the wild-type strain.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"55-62"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Microbiota Profiling by CLIN-MALDI-TOF-MS: Distinct Representative Species Across Sites. 通过临床- maldi - tof - ms分析口腔微生物群：不同部位的独特代表性物种。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097814

Yang Jia Liu, Cai Ping Ma, Feng Chen

{"title":"Oral Microbiota Profiling by CLIN-MALDI-TOF-MS: Distinct Representative Species Across Sites.","authors":"Yang Jia Liu, Cai Ping Ma, Feng Chen","doi":"10.3290/j.cjdr.b6097814","DOIUrl":"10.3290/j.cjdr.b6097814","url":null,"abstract":"Objective: To establish a rapid and high-throughput clinical matrix-assisted laser desorption ionisation-time of flight mass spectrometry (CLIN-MALDI-TOF-MS) method for identifying oral microorganisms and to determine the distinct representative species across various oral sites.Methods: Samples were collected from 54 volunteers from four oral sites: saliva, supragingival plaque, oral mucosa and dorsum of the tongue. Microorganisms were cultured on brain heart infusion (BHI) plates and identified using CLIN-MALDI-TOF-MS after processing with specific reagents for mass spectrometry.Results: The method identified 15 species and 12 genera of microorganisms, revealing significant differences in microbial composition among the oral sites, and different oral cavity sites harboured distinct relatively representative species.Conclusion: The CLIN-MALDI-TOF-MS method offers a rapid and efficient approach for large-scale microbial identification in the oral cavity, providing a suitable approach for future experimental teaching and highlighting the importance of site-specific microbial communities in oral health research.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Management of Impacted Teeth in Cystic Lesions of the Jaws. 颌骨囊性病变阻生牙的综合治疗。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097603

Yan Fang Sun, Qian Ling Wang, Zhuo Yue Shi, Yi Zhao

{"title":"Comprehensive Management of Impacted Teeth in Cystic Lesions of the Jaws.","authors":"Yan Fang Sun, Qian Ling Wang, Zhuo Yue Shi, Yi Zhao","doi":"10.3290/j.cjdr.b6097603","DOIUrl":"10.3290/j.cjdr.b6097603","url":null,"abstract":"Cystic lesions in the jaws are frequently associated with impacted teeth, and include dentigerous cysts, odontogenic keratocysts, unicystic ameloblastoma and adenoid odontogenic tumours. The most common treatment modality is enucleation of cysts with removal of the impacted tooth. Marsupialisation is a more conservative treatment modality than enucleation and is considered the first-line treatment, especially in the initial management of benign cystic lesions during the mixed dentition period. Depending on the size of the lesion, the position of the impacted tooth and the available space, the majority of teeth can erupt spontaneously after marsupialisation. A multidisciplinary approach has been used in recent years for management of these lesions. Orthodontic traction is sometimes performed on the impacted tooth to guide tooth eruption postoperatively. When an impacted tooth or teeth within cystic lesions are preserved and functional occlusion is obtained, the patient's quality of life can improve significantly. Prospective clinical trials with a larger patient cohort are necessary to determine the clinical benefit of the conservative approach with marsupialisation or surgical-orthodontic treatment of impacted teeth in cystic lesions since only studies of small groups of patients or case reports have been published to date.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"19-30"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Differential Translation Profiles of Human Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation. 人骨髓间充质干细胞成骨分化过程中差异翻译谱分析。

The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) Pub Date : 2025-03-31 DOI: 10.3290/j.cjdr.b6097608

Hua Liu, Zhi Peng Fan, Qiu Bo Yang, Hui Na Liu

{"title":"Analysis of Differential Translation Profiles of Human Bone Marrow Mesenchymal Stem Cells during Osteogenic Differentiation.","authors":"Hua Liu, Zhi Peng Fan, Qiu Bo Yang, Hui Na Liu","doi":"10.3290/j.cjdr.b6097608","DOIUrl":"10.3290/j.cjdr.b6097608","url":null,"abstract":"Objective: To explore the differential translation profiles and coding products of human jaw bone marrow mesenchymal stem cells (h-JBMMSCs) during osteogenic differentiation.Methods: Ribo-seq was used to examine the differential translated genes (DEGs), open reading frames (ORFs) and genes associated with the osteogenic differentiation phase of h-JBMMSCs. Western blotting (WB) was performed to detect the expression of osteocalcin (OCN) and bone sialoprotein (BSP). Alkaline phosphatase (ALP) activity and alizarin red staining (ARS) were used to detect osteogenic differentiation. A lentivirus containing 5'UTR-ORF-GFPmut was designed to transfect h-JBMMSCs, and fluorescence and green fluorescent protein (GFP) expression were analysed. The SNHG1 peptide was synthesised for osteogenic induction and to detect osteogenic markers.Results: A total of 53,432 ORFs were detected and 199 candidate translation sORFs, including lncRNA SNHG1, were identified after removing the annotated protein-coding genes. In addition, the 5'UTR-ORF-GFPmut showed green fluorescence and expressed GFP. Knockdown of the lncRNA SNHG1 increased the ALP activity of h-JBMMSCs, promoted the expression of OCN and BSP, and enhanced the intensity of ARS and calcium ion content. However, overexpression of lncRNA SNHG1 and the SNHG1 polypeptide inhibited the osteogenic differentiation of h-JBMMSCs.Conclusion: LncRNA SNHG1 inhibited the osteogenic differentiation of h-JBMMSCs. LncRNA SNHG1 can encode a peptide of 19-amino acid and inhibit the osteogenic differentiation of h-JBMMSCs.","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"28 1","pages":"31-43"},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0