Ji Hao Sun, Fei Chen, Koji Kanefuji, Abu Faem Mohammad Almas Chowdhury, Ricardo Marins Carvalho, Hidehiko Sano
{"title":"Application of a New Microtensile Bond Strength Testing Technique for the Evaluation of Enamel Bonding.","authors":"Ji Hao Sun, Fei Chen, Koji Kanefuji, Abu Faem Mohammad Almas Chowdhury, Ricardo Marins Carvalho, Hidehiko Sano","doi":"10.3290/j.cjdr.b1965031","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1965031","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate adhesives' enamel bonding performance utilising the traditional microtensile bond strength test (µTBST) and a new double-sided microtensile bond strength test (DµTBST) to assess the suitability of the latter.</p><p><strong>Methods: </strong>A 'tug-of-war' direct encounter design was employed to compare the enamel bond strengths of two universal adhesives and their different application modes simultaneously under the same tensile load applied to double-sided bonded specimens. Clearfil Universal Bond (CU; Kuraray, Kurashiki, Japan) and Scotchbond Universal Adhesive (SB; 3M ESPE, St Paul, MN, USA) were applied in self-etch (S) and etch-and-rinse (E) mode on 110 human molar samples to perform two experiments. Experiment 1 compared the enamel bond strengths of the combinations of adhesive application modes utilising µTBST. The data were analysed using a Welch analysis of variance (ANOVA), followed by a Games-Howell test. Experiment 2 employed DµTBST to determine the suitability of the new double-sided bonded assembly and ascertain which of the adhesive application mode combinations was superior. The data were analysed using a Kaplan-Meier survival analysis, followed by pairwise comparisons with a Mantel-Cox log-rank test. The level of significance was set at P ˂ 0.05.</p><p><strong>Results: </strong>The µTBST results did not show significant differences for CUE vs CUS, SBE vs SBS, CUS vs SBS and CUS vs SBE (P ˃ 0.05); however, from DµTBST, the survival distributions for the interventions were statistically significantly different (χ2(3) = 145.130, P ˂ 0.0005), indicating the superiority of universal adhesive CU over SB and application mode E over S with certainty.</p><p><strong>Conclusion: </strong>DµTBST was able to add more discerning outcomes to the µTBST results, indicating that the new technique could become a valuable adjunct to the conventional method.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 3","pages":"159-166"},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39391252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lateral Lingual Foramina and Canals in the Mandible: CBCT Evaluation of 506 Patients in North China.","authors":"Mu Qing Liu, Ke Jia Chen, Kai Yuan Fu","doi":"10.3290/j.cjdr.b1964993","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1964993","url":null,"abstract":"<p><strong>Objective: </strong>To assess the prevalence, location, diameter, course and anastomosis of the lateral lingual foramina (LLF) and canals (LLCs) in a northern Chinese population using CBCT.</p><p><strong>Methods: </strong>CBCT images of 506 patients (181 male and 325 female, mean age 21.03 ± 8.11 years) were collected. The prevalence, location, diameter, length, angle and anastomosis of the LLF and LLCs were assessed. The measurement variables were analysed by sex and age. Statistical analysis was performed using SPSS (v. 25, IBM, Armonk, NY, USA).</p><p><strong>Results: </strong>A total of 461 LLF were detected in 307 (60.7%) subjects, 175 (57.0%) of whom had unilateral LLF and 132 (43.0%) of whom had bilateral LLF, with each lateral having one to four LLF. The majority of LLF (375/461, 81.3%) were located below the premolars, particularly the first premolar. The mean diameter of the LLF was 0.58 ± 0.20 mm. The mean vertical distance from the LLF to the inferior border and the alveolar crest was 6.68 ± 1.43 mm and 23.65 ± 2.89 mm, respectively. In total, 197 LLCs were visible in the cancellous bone and evaluated. The mean length of LLCs was 6.26 ± 1.29 mm, and the mean angle of LLCs was 140.64° ± 17.29°. The overwhelming majority (93.4%) of LLCs communicated with the mandibular incisive canal and the rest connected with the mandibular canal.</p><p><strong>Conclusion: </strong>The prevalence of LLCs was high in the northern Chinese population. The presence of LLCs is a significant predictor of communication with the mandibular incisive canal.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 3","pages":"177-183"},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39391254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rui Fang Lu, Li Xu, Xiang Hui Feng, Xian E Wang, Huan Xin Meng
{"title":"Multilevel Analysis of Non-surgical Periodontal Treatment of Patients with Generalised Aggressive Periodontitis.","authors":"Rui Fang Lu, Li Xu, Xiang Hui Feng, Xian E Wang, Huan Xin Meng","doi":"10.3290/j.cjdr.b1965015","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1965015","url":null,"abstract":"<p><strong>Objective: </strong>To investigate various factors affecting the clinical outcome of nonsurgical periodontal treatment and evaluate the treatment effects of adjunctive amoxicillin and metronidazole (AMX + MET) in patients with generalised aggressive periodontitis (GAgP).</p><p><strong>Methods: </strong>Forty-two patients with GAgP were recruited and randomly assigned to three groups: scaling and root planing (SRP) only, AMX + MET after SRP, and AMX + MET during SRP. The patients were assessed every 2 months post-therapy. Periodontal clinical and subgingival microbiological parameters were analysed at baseline and 6 months post-therapy. The impacts of different covariates on pocket probing depth (PD) reduction were evaluated.</p><p><strong>Results: </strong>A multilevel analysis revealed that 58% of the variability in PD reduction was attributed to site-level parameters, 27.3% to patient-level parameters and 18.7% to tooth-level parameters. Greater PD reduction can be expected at initially deeper PD sites and sites with intrabony defects, and in patients with adjunctive use of AMX + MET. Persistent Tannerella forsythia infection and tooth mobility after treatment were negatively associated with PD reduction.</p><p><strong>Conclusion: </strong>The clinical outcomes of nonsurgical periodontal treatment were mainly influenced by site-level parameters, and adjunctive use of AMX + MET can lead to better clinical results in patients with GAgP in a short time.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 3","pages":"191-198"},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39391256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"3D Digital Evaluation for Direct Composite Restoration Using the Modified Stamp Technique.","authors":"Kun Qian, Qi Lin Wang, Jie Pan","doi":"10.3290/j.cjdr.b1965019","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1965019","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the clinical performance of direct composite restorations using the modified stamp technique (MST) in vivo.</p><p><strong>Methods: </strong>A total of 30 posterior teeth with undermining caries were randomly divided into two groups and then restored using the MST and conventional technique (CT), respectively. 3D images of the occlusal surface were obtained using an intraoral scanner (CEREC Omnicam, Dentsply Sirona, Charlotte, NC, USA) before and after treatment and the differences between these two 3D images were analysed with reverse engineering software. Statistical analysis was performed using a one-way analysis of variance (ANOVA) combined with a least significant difference (LSD) post hoc test using SPSS 20.0 software (IBM, Armonk, NY, USA).</p><p><strong>Results: </strong>The statistical analysis revealed that the average root mean square (RMS) values of best fit alignment errors were significantly different between groups (P < 0.01). The average RMS values in the MST and CT groups were 0.0738 ± 0.0279 and 0.1638 ± 0.0682, respectively. The LSD post hoc test revealed that the value was significant smaller in the MST group than in the CT group.</p><p><strong>Conclusion: </strong>The MST was effective in direct composite restoration. The morphological consistency of the occlusal surface using the MST was better than with the CT.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 3","pages":"185-189"},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39391255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Crucial Role of Wntless in Osteogenesis and Odontogenesis.","authors":"Jia Hui Du, Shu Xian Lin, Xin Quan Jiang","doi":"10.3290/j.cjdr.b1530533","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530533","url":null,"abstract":"<p><p>Wnt signalling pathways have been the focus of intense research activity for decades due to their fundamental role in skeletal and dental development. Wntless, an exclusive chaperone protein for the exocytotis of Wnt ligands, was identified in 2006. In the last decade, the molecular biological studies of Wntless and its genetic studies in human and mice have highlighted the importance of this protein in mineralised tissues, including bone, cartilage and teeth. This article reviews recent developments and discrepancies in the role of Wntless in skeletal and dental development based on mutant phenotypes, as well as the underlying mechanism involved in its molecular and physiological regulation. We conclude that, though some controversial phenotypes exist due to different Cre line resources, Cre recombinase activity and detection time points, Wntless undeniably exerts a context- and stage-dependent regulatory function during the development and homeostasis of both skeletal and dental tissue.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"85-94"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Two Post Preparation Methods in Mandibular Second Molars with a C-shaped Root Canal Configuration.","authors":"Si Yu Li, Yi Hong Liu, Ke Yi Hao, Jie Pan","doi":"10.3290/j.cjdr.b1530527","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530527","url":null,"abstract":"<p><strong>Objective: </strong>To compare the reduction of residual dentine thickness of two different post preparation methods on the mandibular second molars with a C-shaped root canal configuration.</p><p><strong>Methods: </strong>A total of 26 extracted right mandibular second molars with a C-shaped root canal configuration were selected and paired based on similar canal morphology. Each of the paired teeth was randomly allocated to the heat and ultrasonic instruments group (HU group) or Peeso Reamer (Mani, Utsonomiya, Japan) group (PR group) (n = 13) and received post preparation with different instruments after the same endodontic treatment. The reduction of residual dentine thickness and the minimal remaining dentine thickness at the apical sections at 4 or 7 mm below the cementoenamel junction (CEJ) were recorded. The data were analysed using an independent samples t test (α = 0.05).</p><p><strong>Results: </strong>The reduction of residual dentine thickness for the HU group was less than that for the PR group in the two sections. Moreover, at the section 7 mm below the CEJ, the teeth reduction of the distolingual wall in the HU group (0.022 ± 0.007 mm) was significantly lower than that in the PR group (0.101 ± 0.013 mm) (P < 0.01).</p><p><strong>Conclusion: </strong>Using heat and ultrasonic instruments to perform post preparation could follow the original canal configuration to save more tooth structure in the remaining root canal wall, minimise the reduction of residual dentine thickness and decrease the incidence of root canal perforation.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"125-132"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui Jing Wu, Meng Lin Cheng, Chun Zi Zhang, Meng Ru Xu, Xiao Li Gao, Shuo Du, Min Ding, Xing Wang, Xi Peng Feng, Bao Jun Tai, De Yu Hu, Huan Cai Lin, Bo Wang, Shu Guo Zheng, Xue Nan Liu, Wen Sheng Rong, Wei Jian Wang, Chun Xiao Wang, Tao Xu, Yan Si
{"title":"Associated Factors of Oral Health-related Quality of Life in Chinese Adolescents Aged 12-15 Years.","authors":"Hui Jing Wu, Meng Lin Cheng, Chun Zi Zhang, Meng Ru Xu, Xiao Li Gao, Shuo Du, Min Ding, Xing Wang, Xi Peng Feng, Bao Jun Tai, De Yu Hu, Huan Cai Lin, Bo Wang, Shu Guo Zheng, Xue Nan Liu, Wen Sheng Rong, Wei Jian Wang, Chun Xiao Wang, Tao Xu, Yan Si","doi":"10.3290/j.cjdr.b1530497","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530497","url":null,"abstract":"<p><p>adolescents aged 12 to 15 years based on the 4th National Oral Health Survey and to explore its associated factors.</p><p><strong>Methods: </strong>Students aged 12 to 15 years were recruited using to a multistage stratified random sampling method. All the subjects received oral examination and completed a questionnaire. Information relating to OHRQoL was collected through a Mandarin version of the child oral impacts on daily performances (Child-OIDP) questionnaire. The relationship between the Child-OIDP scores and independent variables was assessed using a Mann-Whitney U-test, Kruskal-Wallis test and a multivariate Poisson regression.</p><p><strong>Results: </strong>A total of 89,582 subjects were included in the analysis, of whom 76.6% reported oral impacts in the last 6 months. Eating was the most affected daily performance. The results of the regression analysis showed that factors associated with adolescents' OHRQoL included sex, location of residence, region, only child status, parents' level of education, frequency of sugar consumption, self-perception of general/oral health, dental appointments in the past 12 months, oral health knowledge status, age, decayed, missing and filled teeth (DMFT) index and number of teeth with gingival bleeding.</p><p><strong>Conclusion: </strong>Oral impacts were common among Chinese adolescents, although most were not so severe. Eating was the most commonly affected performance. Sex, location of residence, region, only child status, parents' level of education, frequency of sugar consumption, selfperception of general/oral health status, dental appointments in the previous 12 months, oral health knowledge status, age, DMFT index and number of teeth with gingival bleeding were found to be associated with OHRQoL.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"105-112"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Surface Treatment Procedures on Bond Strength of Lithium Disilicate Glass Ceramic.","authors":"Peng Yu, Xiao Yan Wang","doi":"10.3290/j.cjdr.b1530491","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530491","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the microshear bond strength (μSBS) of resin cement to a lithium disilicate glass ceramic conditioned with different surface treatment procedures.</p><p><strong>Methods: </strong>Crystallised slices of lithium disilicate glass ceramic were randomly divided into five groups (n = 10) according to different surface treatment procedures: the no surface treatment (NT) group was untreated; the hydrofluoric acid (HF) group was conditioned with 4.5% HF; the silane (S) group was conditioned with a silane coupling agent; the hydrofluoric acid and silane (HFS) group was conditioned with HF followed by the silane coupling agent; and the Monobond Etch & Prime (MEP) (Ivoclar Vivadent, Schaan, Liechtenstein) group was conditioned with the one-step self-etching primer MEP. Resin cement was applied to the ceramic surfaces and irradiated. A μSBS test was performed. Failure analysis, surface roughness tests, surface topography examination and elemental analysis were also conducted. The data were analysed with a one-way analysis of variance (ANOVA) and Tukey honestly significant difference test (P < 0.05).</p><p><strong>Results: </strong>The MEP group resulted in comparable μSBS to the HFS group (16.9 ± 4.3 MPa and 16.0 ± 2.2 MPa, respectively), but a significantly higher μSBS than the NT (1.0 ± 0.9 MPa), HF (8.9 ± 3.9 MPa) and S (12.6 ± 2.5 MPa) groups. Adhesive failure was mainly observed in the NT and HF groups, while the S, HFS and MEP groups demonstrated the most mixed failure. Though micrographs revealed a roughened surface in the HF group, no significant difference was found with any other groups.</p><p><strong>Conclusion: </strong>Within the limitations of this study, it can be concluded that the μSBS of resin cement to lithium disilicate glass ceramic etched with MEP is as efficient as that treated with HF and silane.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"119-124"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tian Yu, Dan Ying Tao, Hai Xia Lu, Jia Lin Zhu, Chun Yu Xie, David Bartlett, Xi Ping Feng
{"title":"Prevalence and Associated Factors of Tooth Wear in Shanghai.","authors":"Tian Yu, Dan Ying Tao, Hai Xia Lu, Jia Lin Zhu, Chun Yu Xie, David Bartlett, Xi Ping Feng","doi":"10.3290/j.cjdr.b1530421","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530421","url":null,"abstract":"<p><strong>Objective: </strong>To estimate the prevalence and distribution of tooth wear among groups of adolescents and adults in Shanghai, China through an epidemiological survey, and explore the associated factors.</p><p><strong>Methods: </strong>Multistage, stratified, constant volume and cluster sampling methods were used in an epidemiological survey conducted in Shanghai in 2014. The basic erosive wear examination (BEWE) index was used to screen for tooth wear in different age groups: 12 years, 15 years, 18 to 35 years, 36 to 49 years and 50 to 74 years. A previously published questionnaire collected information including dietary habits, oral hygiene habits and general conditions.</p><p><strong>Results: </strong>This survey reports the results for 1806 participants in Shanghai. The prevalence of tooth wear was 59.7% in adolescents (BEWE ≥ 1) and 93.1% in adults (BEWE ≥ 2). The prevalence and severity of disease increased with age (P < 0.01). The teeth most susceptible to wear were the central incisors and first molars. Multivariate analysis of covariance (ANCOVA) results showed that soft drinks, alcoholic drinks, pickled vegetables and hard food, gastroesophageal reflux disease (GERD), xerostomia and poor tooth brushing habits were statistically correlated with tooth wear in different age groups.</p><p><strong>Conclusion: </strong>The prevalence of tooth wear appears to be high in adolescents and adults in Shanghai. Frequent consumption of soft or alcoholic drinks, GERD, xerostomia and poor tooth brushing habits were positively associated with tooth wear in different age groups.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"95-103"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Different Irrigation Activation Techniques on Sealer Penetration: A Confocal Laser Microscopy Study.","authors":"Zeliha Uğur Aydin, Sevim Koşumcu, Büşra Meşeci","doi":"10.3290/j.cjdr.b1530507","DOIUrl":"https://doi.org/10.3290/j.cjdr.b1530507","url":null,"abstract":"<p><strong>Objective: </strong>To compare the efficiency of a new sonic powered irrigation system named EDDY (VDW, Munich, Germany), passive ultrasonic irrigation (PUI) and conventional needle irrigation (CNI) in root canal sealer penetration.</p><p><strong>Methods: </strong>A total of 45 mandibular premolars were instrumented up to size 30, 0.9 taper and randomly divided into three groups (n = 15) depending on the final irrigation activation technique: EDDY, PUI or CNI. After the final irrigation procedures, the root canals were obturated with labelled sealer mixed with 0.1% rhodamine B. Transverse sections at 3, 5 and 7 mm from the root apex were examined using confocal laser scanning microscopy. The maximum depth and total area and percentage of sealer penetration were measured using ImageJ analysis software (National Institutes of Health, Bethesda, MD, USA).</p><p><strong>Results: </strong>In the EDDY group, the penetration depth was higher compared to the CNI group in the apical and middle sections and compared to the PUI group in the apical section (P ˂ 0.05). The penetration area in the EDDY group was higher compared to the CNI group in all sections and compared to the PUI group in the coronal section (P ˂ 0.05). The percentage of penetration was higher in the EDDY group compared to the CNI group in all sections and compared to the PUI group in the coronal section (P ˂ 0.05).</p><p><strong>Conclusion: </strong>In the present study, sealer penetration was superior in the EDDY group than the CNI group in the apical section. In the middle and coronal sections, sealer penetration was similar for the EDDY and PUI groups.</p>","PeriodicalId":74983,"journal":{"name":"The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)","volume":"24 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39148012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}