Agricultural Sciences in China最新文献

{"title":"Effects of Chronic Stress of Cadmium and Lead on Anatomical Structure of Tobacco Roots","authors":"Zu-li YUAN ,&nbsp;Shu-ping XIONG ,&nbsp;Chun-ming LI ,&nbsp;Xin-ming MA","doi":"10.1016/S1671-2927(11)60195-8","DOIUrl":"10.1016/S1671-2927(11)60195-8","url":null,"abstract":"<div><h3>Abstract</h3><p>Cadmium (Cd²⁺) is one of the major widespread environmental pollutants, and can cause serious problems to all organisms. Lead (Pb²⁺) is another wide spread dangerous heavy metal. Tobacco is a popular growing economic crop in China. Most tobacco growing region soils contain excessive Cd²⁺ and Pb²⁺. To assess anatomic changes of tobacco roots under Cd²⁺, Pb²⁺, and Cd²⁺+Pb²⁺ chronic stress, a pot experiment was carried out in field. The tobacco seedlings with 6 leaves were transplanted to pots in which soil was placed. The amounts of Cd²⁺ added to soil were 0, 3, 6, 10, 30, 60, and 100 mg kg⁻¹ dry soil. The amounts of Pb²⁺ added to soil were 0, 150, 300, 450, 600, 750, and 1 000 mg kg⁻¹ dry soil. The amounts of Cd²⁺+Pb²⁺ added to soil were 0+0, 3+150, 6+300, 10+450, 30+600, 60+750, and 100+1 000 mg kg⁻¹ dry soil. The contents of Cd²⁺ and Pb²⁺ in root systems were determined by inductively coupled plasma, and the anatomical structure was studied by method of paraffin sectioning. The results revealed that the amounts of exchangeable Cd²⁺ and Pb²⁺ and carbonate bound Cd²⁺ and Pb²⁺ in soil increased with the amounts of Cd²⁺ and Pb²⁺ added to soil, and the contents of both Cd²⁺ and Pb²⁺ in roots were significantly increased along with stress time and the amounts of Cd²⁺ and Pb²⁺ added to soil. The growing of tobacco in Cd²⁺ and Cd²⁺+Pb²⁺ polluted soil for 50, 100, and 150 d resulted in some abnormal external morphological and anatomical changes in ripe region of lateral roots. All the abnormal roots had abnormal vascular cylinders, and the ratio of abnormal external morphological and anatomical changes of roots positively correlated with the Cd²⁺ contents in roots and stress time. While, there were no abnormal external morphological and anatomical changes of roots under Pb²⁺ stress. It was suggested that Cd²⁺ stress could cause abnormal anatomic changes of roots, but Pb²⁺ stress could not.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1941-1948"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60195-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca2+ Influx During Sperm Hyperactivation","authors":"Zhen ZHANG,&nbsp;Xuan ZHOU,&nbsp;Hui-xia LI,&nbsp;Qun-wei CUI,&nbsp;Jing YU,&nbsp;Gen-lin WANG","doi":"10.1016/S1671-2927(11)60197-1","DOIUrl":"10.1016/S1671-2927(11)60197-1","url":null,"abstract":"<div><h3>Abstract</h3><p>CatSper is a unique Ca²⁺ channel-like protein family exclusively expressed in the testis and sperm, and plays important roles in sperm motility, capacitation, acrosome reaction and sperm-egg interactions. Here we studied the mechanism of regulation of CatSper2-dependent Ca²⁺ influx, extracellular and intracellular Ca²⁺ on sperm hyperactivated motility. The siRNA duplexes were transfected into the sperm cells by electroporation (EP) to silence the expression of CatSper2. The results for targeted disruption of CatSper2 showed the highest silence efficiency 77.7% (P&lt;0.05), the hyperactivated sperm rate calculated by computer-assisted semen analysis (CASA) analysis of interferenced sperm was significantly lower 11.1% than the control 99.2%. Flow cytometry (FCM) detection of the intracellular Ca²⁺ concentration of interferenced sperm was 50 nmol L⁻¹ higher than the normal. It was remarkably lower than hyperactivated sperm with 200-1 000 nmol L⁻¹ (P&lt;0.05). It was not sufficient to evoke hyperactivation. To trigger hyperactivation, the sperm were incubated in 50 mol L⁻¹ thimerosal or 5 mmol L⁻¹ procaine, it sharply increased the intracellular Ca²⁺ <em>via</em> two different channels. CASA and FCM detection indicated that intracellular Ca²⁺ is required for initiating hyperactivation while extracellular Ca²⁺ is essential to maintain the process. We concluded that to mediate sperm hyperactivation, it is essential to inhibit Ca²⁺ peak present with targeted disruption of CatSper2 expression. This provides important prospective for further exploration of signal channel of sperm hyperactivated motility, potential factors for male infertility and provide further reference to the exploration of male contraceptive drug targets of male reproduction.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1958-1967"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60197-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated Quantitative Trait Locus Map of Oil Content in Soybean, Glycine max (L.) Merr., Generated Using a Meta-Analysis Method for Mining Genes","authors":"Zhao-ming QI ,&nbsp;Xue HAN ,&nbsp;Ya-nan SUN ,&nbsp;Qiong WU ,&nbsp;Da-peng SHAN ,&nbsp;Xiang-yu DU ,&nbsp;Chun-yan LIU ,&nbsp;Hong-wei JIANG ,&nbsp;Guo-hua HU ,&nbsp;Qing-shan CHEN","doi":"10.1016/S1671-2927(11)60166-1","DOIUrl":"10.1016/S1671-2927(11)60166-1","url":null,"abstract":"<div><h3>Abstract</h3><p>Soybean is a major cash crop in the world, and its oil content was one of the very important traits. Therefore, the study of gene mapping for oil content in soybean is very important for breeding application. At present, at least 130 QTL loci for soybean oil content have been published; however, the mapping results of oil content were dispersed and a coalescent public map should be established to integrate the published QTLs, and to more efficiently mine genes based on the meta-analysis method of the bioinformatics tools. This study was to construct an integrated map of QTLs for soybean oil content and accelerate the application of bioinformation resource related to oil content improvement in the practice of soybean breeding. We collected information of 130 QTLs reported over the past 20 yr for soybean oil content and used the Software BioMercator 2.1 to project QTLs from their own maps onto a reference map, which was an early-integrated map constructed by Song (2004) for oil-content quantitative trait loci (QTLs) in soybean. Gene mining was performed based on the meta-analysis by running the local ver. GENSCAN and InterProScan. The confidence interval of QTLs was efficaciously narrowed using the meta-analysis method, and 25 consensus QTLs were mapped on the reference map. Using a local version of GENSCAN, 12 805 sequences in the consensus QTL intervals were predicted. With BLAST, these predicted sequences were aligned to gene sequences from the International Protein Index database using InterProScan locally. Thirteen predicted genes were in the class of the geme ontology (GO) accession (0006631), which were involved in the fatty acid metabolic process. These genes were analyzed using BLAST at the NCBI website to examine whether they were related to oil content. Six genes were found in the oil-synthesis pathway. Twenty-five consensus QTLs and six genes were found in the oil-synthesis pathway. These results would lay the foundation for marker-assisted selection and mapping QTL precisely, and these genes will facilitate the researches on the gene mining of oil synthesis and molecular breeding in soybean.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1681-1692"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60166-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Age on Quality of Beef from Qinchuan Cattle Carcass","authors":"Lin-qiang LI ,&nbsp;Wan-qiang TIAN ,&nbsp;Lin-sen ZAN","doi":"10.1016/S1671-2927(11)60176-4","DOIUrl":"10.1016/S1671-2927(11)60176-4","url":null,"abstract":"<div><h3>Abstract</h3><p>Qinchuan cattle are one of the top breeds in China. In the current study, cattle of different ages were used to investigate the effect of age on beef quality. With increasing age, shear force, protein content, hydroxyproline (Hyp) content, redness (a*), and muscle fiber diameter and density significantly increased (<em>P</em>&lt;0.05). On the other hand, cooking loss, water content, lightness (L*), and muscle fiber gap markedly decreased (<em>P</em>&lt;0.05). No significant differences were found in the contents of intramuscular fat, ash, amino acids (AA), mineral elements, and expression level of leptin and FAS (<em>P</em>&gt;0.05). The C16:0 and C18:0 content increased significantly (<em>P</em>&lt;0.05), in contrast to that of C18:1 and C18:2 (<em>P</em>&lt;0.05). COLA1 mRNA was expressed at low levels at mon 3 and 9 but markedly increased at mon 12 and 15 (<em>P</em>&lt;0.05). Taken together, these observations demonstrate excellent nutritional values of beef from cattle of different ages concordant with their chemical properties; on the other hand, their commercial value correlates more closely with their physical and sensory characteristics.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1765-1771"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60176-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23","authors":"Mao-sheng CUI ,&nbsp;Zhen-xing LIU ,&nbsp;Xian-long WANG ,&nbsp;Jing ZHANG ,&nbsp;Yi WU ,&nbsp;Guo-cai HAN ,&nbsp;Shen-ming ZENG","doi":"10.1016/S1671-2927(11)60177-6","DOIUrl":"10.1016/S1671-2927(11)60177-6","url":null,"abstract":"<div><h3>Abstract</h3><p>The developmental competence of porcine parthenotes cultured in porcine zygote medium-3 (PZM-3) and North Carolina State University-23 (NCSU-23) media was investigated. After <em>in vitro</em> maturation oocytes were electro-activated, and the subsequent developmental competence, rates of apoptotic, fragmented and arrested embryos from the two media were evaluated. Also, the ratio of mRNA expression of Bcl-2 and Bax gene was determined. Results demonstrated that cleavage, blastocyst, hatched blastocyst rates, and blastocyst cell numbers were significantly higher in PZM-3 than in NCSU-23. The rate of fragmented embryos in PZM-3 was lower than in NCSU-23 on d 1 and 3 (<em>P</em>&lt;0.05) while the percentage of arrested embryo was lower in PZM-3 than in NCSU-23 on d 4 and 5 (<em>P</em>&lt;0.05). TUNEL positive signals were higher in NCSU-23 than in PZM-3 from d 3 to 7 (<em>P</em>&lt;0.05). The ratios of Bcl-2 and Bax mRNA expression in embryos were higher on d 5 than on d 3 and 1 in PZM-3 (<em>P</em>&lt;0.05). In contrast, the ratios of Bcl-2 and Bax mRNA expression in embryos on d 1 were higher than on d 3 and 5 in NCSU-23 (<em>P</em>&lt;0.05). Additionally, the ratios of Bcl-2 and Bax mRNA expression in embryos in PZM-3 were higher than in NCSU-23 on d 3 and 5 (<em>P</em>&lt;0.05). In conclusion, lower apoptotic embryo rates and down-regulating Bax together with up-regulating expression of Bcl-2 transcripts may be responsible for the better developmental competence of porcine parthenotes in PZM-3.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1772-1780"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60177-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation and Immunogenicity of a Recombinant Adenovirus Co-Expressing the E2 Protein of Classical Swine Fever Virus and the GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus","authors":"Hong-yu LI,&nbsp;Yuan SUN,&nbsp;Xing-juan ZHANG,&nbsp;Tian-ming CHANG,&nbsp;Xiang-peng WANG,&nbsp;Fan HE,&nbsp;Jun-hua HUANG,&nbsp;Hua-ji QIU","doi":"10.1016/S1671-2927(11)60178-8","DOIUrl":"10.1016/S1671-2927(11)60178-8","url":null,"abstract":"<div><h3>Abstract</h3><p>Classical swine fever (CSF) and porcine reproduction and respiratory syndrome (PRRS) are both economically important, highly contagious diseases of swine worldwide. To develop an effective vaccine to control these two diseases, we constructed a recombinant adenovirus rAdV-GP52AE2, using a replication-defective human adenovirus serotype 5 as a delivery vector, to co-express the GP5 protein of highly pathogenic porcine reproduction and respiratory syndrome virus (PRRSV) and the E2 protein of classical swine fever virus (CSFV). Foot-and-mouth disease virus (FMDV) 2A peptide was used as a linker between the GP5 and E2 proteins to allow automatic self-cleavage of the polyprotein. The GP5 and E2 genes were expressed as demonstrated by immunofluorescence assay and Western blotting. Immunization of mice resulted in a CSFV-neutralizing antibody titer of 1:128 and a PRRSV-neutralizing antibody titer of 1:16. The lymphoproliferative responses were detected by Cell Counting Kit-8 assay and the stimulation index of CFSV-specific and PRRSV-specific lymphocytes in the rAdV-GP52AE2 group was significantly higher than that in the negative control group. The results show that rAdV-GP52AE2 can induce both effective humoral and cell-mediated immune responses in mice. The protective efficacy of the recombinant virus against CSF was evaluated in immunized rabbits, which were protected from fever induced by challenge with C-strain. Our study provides supporting evidence for the use of FMDV 2A to develop a bivalent genetically-engineered vaccine.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1781-1791"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60178-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical Price Transmission in the China's Layer Industry Chain: an Application of FDL Approach","authors":"Shi-wei XU,&nbsp;Xiao-xia DONG,&nbsp;Zhe-min LI,&nbsp;Gan-qiong LI","doi":"10.1016/S1671-2927(11)60181-8","DOIUrl":"10.1016/S1671-2927(11)60181-8","url":null,"abstract":"<div><h3>Abstract</h3><p>This paper examines the vertical price relationship between upstream and downstream products in China's layer industry chain by estimating elasticity coefficients of the price transmission. We use cointegration tests, error correction models and finite distributed lag models to analyze vertical price shifts between corn, layer feed, egg-laying chicken and egg prices. With monthly data from 1994 to 2010, our results show that various prices in China's layer industry chain are well integrated. In addition, our results indicate that both long-run and short-run price relationships between upstream and downstream products in layer industry chain exist and the long-run equilibrium, to a certain extent, may eliminate the short-run dynamics price deviation. We also found that the influence of corn and feed prices on egg prices is still more remarkable than egg-laying chicken prices. Our findings imply that governments should pay more attention to corn and layer feed prices in order to stabilize egg prices under the conditions of market integration.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1812-1823"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60181-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage","authors":"Biao-lin HU ,&nbsp;Xue-qin FU ,&nbsp;Tao ZHANG ,&nbsp;Yong WAN ,&nbsp;Xia LI ,&nbsp;Yun-hong HUANG ,&nbsp;Liang-fang DAI ,&nbsp;Xiang-dong LUO ,&nbsp;Jian-kun XIE","doi":"10.1016/S1671-2927(11)60164-8","DOIUrl":"10.1016/S1671-2927(11)60164-8","url":null,"abstract":"<div><h3>Abstract</h3><p>Drought stress is one of the major constraints to rice (<em>Oryza sativa</em> L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) <em>Oryza rufipogon</em> Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield <em>via</em> transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC₁F₅ of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1653-1664"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60164-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contents of D-chiro-Inositol, Vitexin, and Isovitexin in Various Varieties of Mung Bean and Its Products","authors":"Yang YAO,&nbsp;Xu-zhen CHENG,&nbsp;Gui-xing REN","doi":"10.1016/S1671-2927(11)60169-7","DOIUrl":"10.1016/S1671-2927(11)60169-7","url":null,"abstract":"<div><h3>Abstract</h3><p>Mung bean (<em>Vigna radiata</em> L.) is rich in bioactive compounds including D-<em>chiro</em>-inositol (DCI), vitexin, and isovitexin, which have beneficial effects on patients with diabetes. To find a better source for these valuable chemicals, we have collected 110 varieties of mung bean seed samples and 8 mung bean products to determine the levels of these bioactive compounds. We also measured the DCI content in mung bean sprouts at different germination stages. Content of DCI, vitexin, and isovitexin in all mung bean varieties ranged from 0.43 to 5.79, 0.12 to 3.00, and 0.03 to 1.16 mg g⁻¹, respectively. The varieties of C0001321, C0003522, and C0004485 have the highest DCI, vitexin, and isovitexin contents, respectively. The mung bean products in the market contained relatively lower level of these bioactive components. Contents of DCI, vitexin, and isovitexin in all mung bean products ranged from 0.119 to 0.717, 0 to 0.547, and 0 to 0.923 mg g⁻¹, respectively. During the 112 h of germination test, DCI level steadily increased at first stage and reached the highest level at 80 h of germination (4.79 mg g⁻¹). These results provide useful information for the selection of suitable varieties and proper germination stages to obtain functional ingredients from mung beans.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1710-1715"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60169-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth","authors":"Deng-xia YI,&nbsp;Lei CUI,&nbsp;Yu-mei LIU,&nbsp;Mu ZHUANG,&nbsp;Yang-yong ZHANG,&nbsp;Zhi-yuan FANG,&nbsp;Li-mei YANG","doi":"10.1016/S1671-2927(11)60167-3","DOIUrl":"10.1016/S1671-2927(11)60167-3","url":null,"abstract":"<div><h3>Abstract</h3><p>To obtain transgenic cabbage line with broad insect resistance, a new synthetic <em>Bacillus thuringiensis cry1Ba3</em> gene was introduced into white cabbage <em>via Agrobacterium tumefaciens</em>-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that <em>cry1Ba3</em> was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that <em>cry1Ba3</em> was expressed. Western blot results confirmed the production of insecticidal protein encoded by <em>cry1Ba3</em>. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1693-1700"},"PeriodicalIF":0.0,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60167-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56762173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}