Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth
引用次数: 27
Abstract
To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
用Bt cry1Ba3基因转化白菜防治小菜蛾的研究
摘要为获得具有广泛抗虫性的转基因白菜品系，通过农杆菌介导转化，将合成的苏云金芽孢杆菌cry1Ba3基因导入白白菜中，获得了37个转化子。聚合酶链反应(PCR)和Southern blot分析证实，cry1Ba3已成功插入白菜基因组。逆转录聚合酶链反应(RT-PCR)证实cry1Ba3表达。Western blot结果证实产生了cry1Ba3编码的杀虫蛋白。昆虫生物测定结果表明，转基因白菜能有效控制易感和抗cry1ac小菜蛾(DBM)幼虫。
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