Reproduction and breeding最新文献

{"title":"Sex biased expression of Fem-1 in larval stages suggests its function in early sex differentiation of Litopeneaus vannamei","authors":"Yang Yu ,&nbsp;Yue Wang ,&nbsp;Xinjia Lv ,&nbsp;Shihao Li ,&nbsp;Fuhua Li","doi":"10.1016/j.repbre.2023.09.004","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.09.004","url":null,"abstract":"<div><p>The Pacific white shrimp <em>Litopenaeus vanname</em>i (<em>L. vanname</em>i) is an economically important species worldwide. As females exhibit a faster growth rate than males, it is crucial to understand the mechanism of sex determination and differentiation for the purpose of mono-sex breeding in shrimp. The <em>feminization-1</em> gene (<em>Fem-1</em>) serves as a sex-determining gene in <em>Caenorhabditis elegans</em>, and the <em>Fem-1</em> family is implicated in sex development in several crustaceans. In this study, three <em>Fem-1</em> family members, namely <em>LvFem-1a</em>, <em>LvFem-1b</em>, and <em>LvFem-1c</em>, were identified and characterized in <em>L. vanname</em>i. Based on genetic sex marker, individuals were separated by sex during the early developmental stages. The expression profiles of these genes were analyzed in female or male larvae. Significant differences between females and males were observed during the early stages of development. In Zoea III or earlier stages, the expressions levels in males were significantly higher than those in females. Intriguingly, there was a shift towards higher expression in females during the mysis and post-larval stages. These findings indicate that the Zoea III stage may be crucial for sex differentiation in shrimp. In addition, the analysis of gene expression across various ovarian stages and tissues was conducted. Results showed that all three genes were highly expressed in the ovary, with particularly elevated levels in ovarian development stage II. These findings suggest that the <em>Fem-1</em> genes play a critical role in early ovary development. This study is the first research focusing on the sex-specific expression of sex differentiation genes in shrimp. The obtained results provide new insights into to the role of the <em>Fem-</em>1 gene family in sex differentiation and gonadal development.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 4","pages":"Pages 153-160"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49725636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of lotus-fish co-culture on the gut microbiome of Hefang crucian carp (Carassis auratus)","authors":"Shengnan Li ,&nbsp;Qianhong Gu ,&nbsp;Zhanxin Li ,&nbsp;Qianqian Zeng ,&nbsp;Hui Zhong ,&nbsp;Meiqi Liu ,&nbsp;Jiaoyu Chen ,&nbsp;Yi Zhou ,&nbsp;Shaojun Liu ,&nbsp;Shengbiao Hu","doi":"10.1016/j.repbre.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.09.001","url":null,"abstract":"<div><p>Gut microbiome plays an important role in host's development and reproduction. Lotus-fish co-culture (LFC) is a popular integrated agriculture–aquaculture farming system in China. However, there is still a relative dearth of information on how changes of aquaculture modes will affect the fish gut microbiome. In the present study, metagenomic sequencing was used to compare gut bacteria of the hybrid fish Hefang crucian carp (<em>Carassius cuuieri</em> ♀ × <em>C. auratus</em> var. red ♂) cultured in LFC and intensive pond culture (IPC) systems. The results indicated that aquaculture modes exerted great influences on the fish gut bacterial community. A significantly higher gut bacterial diversity was found in LFC than that in IPC (<em>P</em> &lt; 0.05). The relative abundances of Actinobacteria and Chloroflexi were apparently higher in LFC than those in IPC, whereas Planctomycetota, Firmicutes, and Verrucomicrobia showed opposite patterns. Besides, gut bacterial metabolic potentials involved in energy metabolism, carbohydrate metabolism, amino acid metabolism, environmental information processing, and cellular processes were significantly increased in LFC compared to those in IPC. On the contrary, the bacterial functional potentials related to nucleotide metabolism, DNA mismatch repair, as well as homologous recombination were significantly stimulated in the IPC system. Source‐tracking analysis suggested that fish gut bacterial community in IPC was originated mainly from water sources, whereas the gut bacterial community in LFC was originated from more unknown sources and soil sources. Our results provided new insight into the influence of aquaculture modes on the microbial community diversity and functionality in aquatic animals and highlighted the importance of environment microbiome in reshaping the gut microbes.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 143-151"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49754922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The kiss2/kiss2r system directly modulates the activity of the pituitary gland in largemouth bass (Micropterus salmoides)","authors":"Wuhui Li ,&nbsp;Yan Miao ,&nbsp;Zexun Zhou ,&nbsp;Shi Wang,&nbsp;Fangzhou Hu,&nbsp;Zhongyuan Shen,&nbsp;Lei Zeng,&nbsp;Jie Hu,&nbsp;Ping Wu,&nbsp;RuRong Zhao,&nbsp;Min Tao,&nbsp;Chun Zhang,&nbsp;Qinbo Qin,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.06.003","DOIUrl":"10.1016/j.repbre.2023.06.003","url":null,"abstract":"<div><p>The kisspeptin system, which involves hypothalamic neuropeptides, plays pivotal roles in the regulation of teleost reproduction. A previous study suggested that the <em>kiss2</em>/<em>kissr2</em> system in largemouth bass (<em>Micropterus salmoides</em>) was involved in regulating gonadal development through the hypothalamus-pituitary-gonad (HPG) axis. However, whether the <em>kiss2</em>/<em>kiss2r</em> system directly regulates the activity of pituitary gland were not studied. This study aimed to elucidate the detailed mechanism of kisspeptin-induced effects on gonadotropin hormone-producing cells in vivo and in vitro. <em>In situ</em> hybridization analysis revealed very widespread expression of <em>kiss2r</em> mRNA in the optic tectum, hypothalamus and pituitary. In the pituitary, <em>kiss2r</em>-expressing cells were mainly located in the proximal pars distalis and pars intermedia, among which the <em>fshβ-</em> and <em>lhβ</em>-positive cells exhibited expression of <em>kiss2r</em> messengers. Furthermore, primary culture of pituitary cells and peptide administration experiments revealed that the Kiss2-10 peptide stimulates the expression of <em>fshβ</em> and <em>lhβ</em> and the secretion of FSH and LH in 24 h. In addition, histology analysis revealed that three injections of the Kiss2-10 peptide promoted ovary development and increased oocyte size but had little effect on testis development. These results suggested that the intrapituitary kisspeptinergic system, as a hypophysiotropic neuropeptide factor, directly modulates of gonadotroph function. This study will help uncover the reproductive endocrinology network and improve artificial breeding technology for largemouth bass.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 99-107"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44858168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human breast organoid models for lactation research","authors":"Jenny Lee ,&nbsp;Yan Liu ,&nbsp;Edward Ray ,&nbsp;Armando E. Giuliano ,&nbsp;Xiaojiang Cui","doi":"10.1016/j.repbre.2023.08.003","DOIUrl":"10.1016/j.repbre.2023.08.003","url":null,"abstract":"<div><p>The human mammary gland is the major organ involved in lactation. In the mammary gland, alveoli secrete milk and myoepithelial cells contract to propel the milk through branched structures called ducts and eventually to the nipple. It is through this process of lactation that infants receive milk, which is essential for proper infant growth and development. The lactation process is comprised of sophisticated interactive networks at the cellular level that are not well understood. Whereas the majority of published mammary gland lactation studies have relied on mouse mammary glands, recent advancements in techniques to study mammary glands enable <em>in vitro</em> reproduction of lactation using human-representative frameworks. Currently, the 3D breast organoid is the state-of-the-art model in human mammary gland research, utilizing induced pluripotent stem cells (iPSCs) or processed patient-derived breast tissues embedded in a special matrix that are then able to grow into complex structures that recapitulate aspects of native human breast tissue. Gaining comprehensive biological insight into the process of lactation through these breast tissue-mimetic 3D models is essential for further studies on lactation-associated human mammary gland diseases, human milk composition, and potential solutions to challenges in maternal milk accessibility. In this short review, the benefits and potential utility of 3D breast organoids in understanding the underlying science of lactation and advancing further human mammary gland studies are discussed.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 125-130"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10239590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary glutathione on growth performance, muscle quality and lipid metabolism of hybrid crucian carp (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) fed a high-fat diet","authors":"Zhongtian Tang ,&nbsp;Chunyan Li ,&nbsp;Gang Xu ,&nbsp;Qiang Zhao ,&nbsp;Zehong Wei ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.07.001","DOIUrl":"10.1016/j.repbre.2023.07.001","url":null,"abstract":"<div><p>A 60-day feeding trial was conducted to investigate the influence of glutathione (GSH) on growth performance, lipid metabolism and muscle quality of hybrid crucian carp (initial body weight: 15.03 ± 0.11 g) fed a high-fat diet (HFD). Five experimental diets were prepared in this study: the control diet (6.17% fat), HFD (12.37% fat), and HFD with 200 mg/kg, 400 mg/kg and 600 mg/kg GSH supplementation, respectively. The results indicated that feeding HFD to hybrid crucian carp remarkably reduced weight gain rate (WGR), specific growth rate (SGR), total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activities of serum and liver, glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities of liver, but significantly increased malondialdehyde (MDA) content of liver compared to the control group. After adding 200–600 mg/kg GSH to HFD, the WGR, SGR, as well as serum and liver T-AOC activity of hybrid crucian carp increased significantly. Moreover, the supplementation of 400 mg/kg GSH significantly increased the activities of T-SOD, catalase (CAT) of serum and liver, as well as the GSH-Px, glutathione-S-transferase (GST) and GR of liver, but decreased MDA content of serum and liver compared to the HFD. The HFD significantly increased muscle crude lipid, polyunsaturated fatty acids (PUFA) contents and fiber diameter, but decreased chewiness, gumminess, hardness and fiber density compared to the control group. The addition of 400–600 mg/kg GSH significantly increased muscle chewiness, gumminess, hardness, and decreased muscle fiber diameter of HFD-fed hybrid crucian carp. Meanwhile, the addition of 200–600 mg/kg GSH significantly reduced the mono-unsaturated fatty acids (MUFA) content and increased the PUFA content of muscle compared to the HFD. The HFD significantly up-regulated the expression of lipid synthesis genes (<em>dgat2</em> and <em>srebp1</em>) and down-regulated the expression of lipolysis genes (<em>lpl</em>, <em>hsl</em> and <em>acox1</em>) of liver compared to the control group. The addition of 400–600 mg/kg GSH significantly decreased the expression of lipid synthesis genes (<em>dgat2</em> and <em>srebp1</em>) and increased the expression of lipolysis genes (<em>hsl, lpl, acox1</em> and <em>pparα</em>) of HFD-fed hybrid crucian carp. This study demonstrated that GSH effectively ameliorated the decline of growth performance and muscle quality of hybrid crucian carp caused by HFD, and reduced the risk of liver lipid deposition of hybrid crucian carp by regulating the expression of genes related to liver lipid metabolism.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 89-98"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44928559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of muscle nutrition and intermuscular bone number in improved diploid carp","authors":"Yahui Chen ,&nbsp;Zhi Xiong ,&nbsp;Peizhi Qin ,&nbsp;Qilong Liu,&nbsp;Yi Fan,&nbsp;Qinglin Xu,&nbsp;Xin Wang,&nbsp;Zhipeng Yang,&nbsp;Wuhui Li,&nbsp;Ming Wen,&nbsp;Fangzhou Hu,&nbsp;Kaikun Luo,&nbsp;Shi Wang,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.08.001","DOIUrl":"10.1016/j.repbre.2023.08.001","url":null,"abstract":"<div><p>The common carp (<em>Cyprinus carpio</em> L., COC, 2n = 100) is one of the most widely consumed and distributed freshwater fish in the world. It ranks fourth in total freshwater aquaculture volume in various regions of China, behind <em>Ctenopharyngodon idella</em>, <em>Hypophthalmichthys molitrix</em>, and <em>Aristichthys nobilis</em>. However, in recent years, environmental degradation, inbreeding, disordered breeding, and other adverse effects have caused problems such as low seed quality and poor disease and stress resistance of the carp. In our laboratory, we developed two types of improved diploid carp the hybrid F₁ of common carp (♀) × blunt snout bream (<em>Megalobrama amblycephala</em>, BSB, 2n = 48) (♂). To investigate the differences between IDC and IDMC compared to COC, in this study we compared the relevant characteristics of these two types of improved carp with those of COC in terms of muscle nutrient composition, intermuscular bone type, and number. The results showed that among the muscle nutrients, IDC had a higher protein content (18.50%) and lower carbohydrate content (0.70%). In addition, the unsaturated fatty acid content of IDC (3.45%) and IDMC (1.25%) were significantly higher than that of COC (<em>P</em> &lt; 0.05) (0.50%). For monounsaturated fatty acids such as oleic acid (OA, C18:1 n-9) and linoleic acid (PA, C16:1), the content of IDC and IDMC were also abundant. In terms of intermuscular bone morphology, the morphology and type of intermuscular bone in IDC and IDMC medullary arch ossicles were consistent with those of COC. However, the number of intermuscular bones was different, and the average number of intermuscular bones of IDMC decreased by 14.77% (<em>P</em> &lt; 0.05) compared with COC. The advantages of IDC and IDMC are different, with IDC having a higher value of muscle nutrients and IDMC having a lower intermuscular bone content. The distant hybridization of common carp (♀) × blunt snout bream (♂) developed two improved diploid carp varieties with excellent traits, which added carp germplasm resources with high muscle nutrition and lower intermuscular bone, and provided theoretical support for their production application.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 118-124"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42364080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of carbohydrate levels on growth, oxidative stress and glucolipid metabolism of hybrid fish between Megalobrama amblycephala (♀) × Culter alburnus (♂) and Culter alburnus","authors":"Jinhai Bai,&nbsp;Chunyan Li,&nbsp;Zhongtian Tang,&nbsp;Chang Wu,&nbsp;Zehong Wei","doi":"10.1016/j.repbre.2023.07.002","DOIUrl":"10.1016/j.repbre.2023.07.002","url":null,"abstract":"<div><p>The present study used hybrid fish (<em>Megalobrama amblycephala</em> ♀ × <em>Culter alburnus</em> ♂, abbreviated as BT) and topmouth culter (<em>Culter alburnus</em>, abbreviated as TC) as research subjects to assess and compare the effects of carbohydrate on the growth, oxidative stress and glucolipid metabolism of the two fish. The TC (initial weight, 13.10 ± 0.10 g) and BT (initial weight, 12.85 ± 0.13 g) were fed isonitrogenous (43% crude protein) and isolipidic (6.5% crude lipid) diets with carbohydrate levels of 10% (LC diet) and 30% (HC diet) for 11 weeks, respectively. The results showed that the HC diet resulted in a significant decrease in specific growth rate (SGR) of both fish. However, the SGR in BT was significantly higher than in TC, regardless of diet. Furthermore, HC diet significantly increased serum alanine transaminase (GPT) and hepatic malondialdehyde (MDA) content, decreased hepatic catalase (CAT) activity and resulted in increased hepatocyte volume, nucleus deficiency and nucleus deviation from the center of cytoplasm in both fish. However, BT had significantly higher glutathione peroxidase (GPx) activity and lower serum GPT and aspartate transaminase (GOT) content compared to TC. The HC diet induced significant increases in hepatosomatic index (HSI), liver lipid droplet area, liver glycogen, serum glucose (GLU) and triglyceride (TG) content in both fish. However, HSI and lipid droplet area were significantly higher in BT than in TC, while serum GLU and TG content were significantly lower in BT than in TC. Both fish can adapt to the HC diet by up-regulating the expression of glycolysis-related gene (glucokinase (<em>gk</em>)) and lipogenesis-related genes (acetyl-CoA carboxylase a (<em>acca</em>), fatty acid synthase (<em>fas</em>)). However, the expression levels of glucose transporter 2 (<em>glut2</em>), glycolysis-related gene (phosphofructokinase liver b (<em>pfklb</em>)) and lipogenesis-related genes (sterol regulatory element-binding protein 1 (<em>srebp1</em>), ATP citrate lyase a (<em>aclya</em>)) were increased only in BT fed HC diet. In summary, the BT liver has stronger glucose transport, glycolysis, and lipid tolerance compared to TC, thus exhibiting better glucose homeostasis and growth performance. It indicates that hybridization may help to produce new strain of fish with high carbohydrate utilization capacity.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 131-142"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45794106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese-superoxide dismutase (MnSOD) rescues redox balance and mucosal barrier function in midgut of hybrid fish (Carassius cuvieri ♀ × Carassius auratus red var ♂) infected with Aeromonas hydrophila and Edwardsiella tarda","authors":"Jie Ou ,&nbsp;Wei-Sheng Luo ,&nbsp;Zi-Rou Zhong ,&nbsp;Qing Xie ,&nbsp;Fei Wang ,&nbsp;Ning-Xia Xiong ,&nbsp;Sheng-Wei Luo","doi":"10.1016/j.repbre.2023.08.002","DOIUrl":"10.1016/j.repbre.2023.08.002","url":null,"abstract":"<div><p>MnSOD is a ubiquitous metalloenzyme that constitutes the first line of antioxidant defense against oxidative stress. In this study, full length sequence of MnSOD was identified from hybrid crucian carp (WR, <em>Carassius cuvieri</em> ♀ × <em>Carassius auratus</em> red var ♂). Tissue-specific analysis revealed that the highest expression of WR-MnSOD was detected in kidney. <em>Aeromonas hydrophila</em> challenge could dramatically enhance WR-MnSOD mRNA expression in tissues. <em>In vivo</em> administration of purified WR-MnSOD peptide could maintain gut mucosal barrier function, rescue redox balance as well as decrease apoptotic cell death in midgut upon bacterial infection, suggesting that WR-MnSOD is playing a crucial role in gut mucosal barrier function and could be used as feed additive to improve gut immunity in fish.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 3","pages":"Pages 108-117"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49600330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapidly generating homozygous mutate zebrafish in F0 generation by technical integration of CRISPR/Cas9 and gynogenesis","authors":"Yunpeng Fan ,&nbsp;Guangjing Zhang ,&nbsp;Kaiyue Zhao ,&nbsp;Xiudan Yuan ,&nbsp;Wen Fu ,&nbsp;Jinhui Liu ,&nbsp;Wenbin Liu ,&nbsp;Liangyue Peng ,&nbsp;Yamei Xiao","doi":"10.1016/j.repbre.2023.04.001","DOIUrl":"10.1016/j.repbre.2023.04.001","url":null,"abstract":"<div><p>Gene editing technique has been widely applied for gene function characterization. However, such an approach is often time-consuming to obtain homozygous mutant. In this article, through timing the earlier injection of embryos, the gene editing efficiency is significantly improved, and together with the technique of the UV inactivated sperm and heat shock treatment for blocking the first cleavage, the gene-edited homozygous zebrafish are successfully obtained in F0 generation, rather than in the third or more generations as reported in the existing results. It is concluded that genetic purification of artificial gynogenesis can play critical role in more efficiently preparing the gene-edited homozygous individuals in fish.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 45-49"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45767698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generating tetraploid zebrafish by heat shock treatment and labeling microtubules of their cells in vitro","authors":"Xiudan Yuan ,&nbsp;Yue Li ,&nbsp;Xiaoli Hu ,&nbsp;Wen Fu ,&nbsp;Ruoyu Lin ,&nbsp;Yunpeng Fan ,&nbsp;Guangjing Zhang ,&nbsp;Jinhui Liu ,&nbsp;Wenbin Liu ,&nbsp;Liangyue Peng ,&nbsp;Yamei Xiao","doi":"10.1016/j.repbre.2023.05.001","DOIUrl":"10.1016/j.repbre.2023.05.001","url":null,"abstract":"<div><p>Tetraploid is a very important experimental material, whether in scientific research or breeding practice. However, tetraploid strains are relatively rare. It was reported that chromosomes allocation disorder during cell division may lead to high embryonic mortality and low fertility in tetraploid fish. In this paper, tetraploid zebrafish is prepared by ploidy operation. And successfully establishes a tetraploid zebrafish cell line <em>in vitro</em>. To tracing status of microtubules, the tetraploid zebrafish cells are transfected with two plasmids of pBLK<em>-α-tubulin-</em>mCherry and pBLK<em>-γ-tubulin-</em>EGFP by electroporation <em>in vitro</em>. Observation shows that these transfected cells can be traced the changes of spindle and centrosomes during cell division in tetraploid cells. The results provide important material platform <em>in vitro</em> for studying the chromosome behavior in tetraploid cell proliferation, especially the formation mechanism of aneuploidy.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 66-71"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43718327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}