Reproduction and breeding最新文献

{"title":"The OsNramp4 aluminum transporter is involved in cadmium accumulation in rice grains","authors":"Xiaohua Hao ,&nbsp;Yifan Mo ,&nbsp;Wenjin Ji ,&nbsp;Xiao Yang ,&nbsp;Zijing Xie ,&nbsp;Dan Huang ,&nbsp;Dongping Li ,&nbsp;Lianfu Tian","doi":"10.1016/j.repbre.2022.10.001","DOIUrl":"10.1016/j.repbre.2022.10.001","url":null,"abstract":"<div><p>Cadmium (Cd) toxicity affects many crops. Members of the <em>OsNramp</em> (<em>Natural resistance-associated macrophage protein</em>) gene family play important roles in the transport of divalent or trivalent cations in rice (<em>Oryza sativa</em> L.). This study explored a possible involvement of OsNramp4 in Cd²⁺ transport in rice. We employed the CRISPR/cas9 technique to obtain rice <em>OsNramp4</em> knockout lines. Using NMT (Non-invasive Micro-test Technology) system for real-time measurement, it was found that the net Cd²⁺ flux was significantly lower than that of wild-type. The yeast strain expressing <em>OsNramp4</em> grew poorly under Cd²⁺ stress, and accumulated more Cd²⁺ than the control strain, which enhanced the sensitivity of yeast to Cd²⁺. OsNramp4 was membrane-localized and mainly expressed in roots, but after tillering, its expression shifted to the nodes and glumes. Furthermore, the loss-of-function <em>OsNramp4</em> mutation lowered the root cell sap Cd²⁺ content, resulting in a significant decrease in Cd²⁺ content in shoot and Cd²⁺ accumulation in grains. We characterized the OsNramp4, an Al³⁺ transporter with the ability to alter the cellular distribution of Cd²⁺ in rice and to reduce Cd²⁺ content in the grain. Our study highlights the complexity of ion uptake and transport in plants.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 125-132"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000345/pdfft?md5=3caaa22a495eda81ddd1cc9f8b348694&pid=1-s2.0-S2667071222000345-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43560781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apical vesicles: Social networking at the pollen tube tip","authors":"Xun Weng,&nbsp;Hao Wang","doi":"10.1016/j.repbre.2022.11.001","DOIUrl":"10.1016/j.repbre.2022.11.001","url":null,"abstract":"<div><p>Pollen tube growth is asymmetric and highly focused at the tip. It is regarded as an ideal model cell system in plants to study polar cell growth and cell polarity. The rapid pollen tube growth is empowered by exocytosis of large amounts of secretion vesicles that contain proteins, lipids and cell wall materials to the apex. Meanwhile, active retrograde endocytosis recycles excessively secreted vesicles for multiple rounds of exocytosis. Meanwhile, it also mediates uptake of extracellular molecules for signal transduction or vacuolar degradation. The dome morphogenesis and fast expansion of the pollen tube tip demand dynamic coordination and balance of antagonistic exocytosis and endocytosis. Nevertheless, the origins, molecular identities and their underlying molecular machineries of the apical vesicles still remain largely uncovered. In this review, we briefly summarize recent progresses in our understanding of the origins and identities of apical vesicles in the pollen tube tip. Furthermore, we focus on discussing elusive aspects underlying the apical vesicles to dominate pollen tube tip growth and highlight a few research challenges that need to be addressed by newly emerging high-resolution bio-imaging and biochemical analysis approaches.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 119-124"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000369/pdfft?md5=35ec4f160b3f61cdd7627dbb8f9bc8b1&pid=1-s2.0-S2667071222000369-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44070557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CrRLK1L receptor kinases-regulated pollen-pistil interactions","authors":"Sihan Gao,&nbsp;Chao Li","doi":"10.1016/j.repbre.2022.11.002","DOIUrl":"10.1016/j.repbre.2022.11.002","url":null,"abstract":"<div><p>Mutual communications and precise recognitions between the male side pollen and female side pistil are crucial for successful fertilization. <em>Catharanthus roseus</em> receptor-like kinases (CrRLK1Ls) participate in pleiotropic biological processes of plant growth, development and defense responses. In this review, we summarize the recent studies on CrRLK1Ls-mediated pollen-pistil interactions, focusing on the key regulatory elements and the underlying molecular mechanisms. Furthermore, the downstream reactive oxygen species, the Ca²⁺ signaling and some important questions are discussed for future directions.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 113-118"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000370/pdfft?md5=f138a9fc7aa702ac3d565c554f8e1877&pid=1-s2.0-S2667071222000370-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43225820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of antagonistic bacteria against Flavobacterium columnus and its effects on growth performance and immune function of Carassius auratus","authors":"Xiaochao Li ,&nbsp;Zhen Huang ,&nbsp;Hui Liu ,&nbsp;Xuejing Wang ,&nbsp;Jiayan Chen ,&nbsp;Liuye Dai ,&nbsp;Shenghua Dong ,&nbsp;Yamei Xiao ,&nbsp;Liping Yang ,&nbsp;Wenbin Liu","doi":"10.1016/j.repbre.2022.10.002","DOIUrl":"https://doi.org/10.1016/j.repbre.2022.10.002","url":null,"abstract":"<div><p>With the popularization of modern intensive farming models, aquatic animals are more prone to outbreaks of various diseases. Due to the abuse of antibiotics, drug resistance among pathogenic bacteria and the deterioration of the water environment continue to occur. Therefore, it is essential to find probiotics that can effectively resist the erosion of pathogenic microorganisms, enhance fish's immunity, and promote fish's growth as an alternative to antibiotics.</p><p>In this experiment, two strains of probiotic bacteria antagonistic to <em>Flavobacterium columnar</em> were isolated from pond water samples. They were identified as <em>Bacillus amyloliquefaciens</em> and <em>Acinetobacter johnsonii</em> by 16S rRNA gene sequence analysis. To further analyze the physiological function of the two probiotics, we added <em>Bacillus amyloliquefaciens</em>, <em>Acinetobacter johnsonii</em> and two strain mixtures to the base feed and fed <em>crucian carp</em> separately. The results found that after 40 days of feeding, the growth performance of wild <em>crucian carp</em> and total protein(TP), globulin(GLO), albumin(ALB) and total bile acid(TBA) had no significant effect. However, <em>Bacillus amyloliquefaciens</em> could significantly reduce total bilirubin(TBiL), direct bilirubin(DBiL), indirect bilirubin(IBiL) and hypersensitive C-reactive protein(hs-CRP) in the serum of <em>crucian carp</em>. The addition of <em>Acinetobacter johnsonii</em> significantly reduced the content of indirect bilirubin(IBiL), acetonitrile/glutamate(A/G) and hs-CRP in the serum of wild <em>crucian carp</em>, which could effectively increase the range of superoxide dismutase(SOD) and reduce the production of malondialdehyde(MDA). After adding the mixed bacteria, Immunoglobulin M(IgM) in the serum of wild <em>crucian carp</em> increased significantly. In addition, the detection of intraperitoneal injection at 24 h, 48 h and 96 h was found to have no significant effect on the serum biochemical indicators of wild <em>crucian carp</em>.</p><p>The above results show that adding <em>Acinetobacter johnsonii</em> can enhance <em>crucian carp</em>'s anti-inflammatory, immunity and antioxidant properties. Therefore, the two bacteria can be used as potential probiotics for wild <em>crucian carp</em>. The research on this topic is of great significance for the screening of water probiotics, improving the water environment, optimizing the intestinal flora of fish, and improving the immunity of fish. At the same time, it provides new ideas for promoting healthy fish farming.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 138-148"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000357/pdfft?md5=92eb4f66f9d1d1a5d5c6873eaeb06fe5&pid=1-s2.0-S2667071222000357-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137281621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptation and integration of environmental cues to internal flowering network in Arabidopsis thaliana","authors":"Zhijuan Chen,&nbsp;Zicong Li","doi":"10.1016/j.repbre.2022.11.003","DOIUrl":"10.1016/j.repbre.2022.11.003","url":null,"abstract":"<div><p>Transition to flowering is timed by environmental cues in plants, which makes plants flower at a favorable time for seed setting. Seasonal cues like cold exposure and day length and environmental abiotic stresses have been found to affect floral transition. In model plant <em>Arabidopsis thaliana</em>, inputs of exogeneous stimuli and multiple internal flowering regulation pathways converge on the FLOWERING LOCUS C (FLC)-FLOWERING LOCUS T (FT) network for flowering. Here, we summarize the current understanding of the epigenetic regulation of flowering in <em>Arabidopsis</em> with the emphasis on the seasonal cues of vernalization and photoperiod pathways. Moreover, the effects of abiotic stresses on hormone-mediated floral transition are briefly discussed.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 133-137"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000382/pdfft?md5=940c76f12c738bd9f18862f18d9d37f7&pid=1-s2.0-S2667071222000382-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45542642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of the texture, composition, antioxidant capacity and nutrients of natural gynogenesis blunt snout bream and its parent muscle","authors":"Ping Wu ,&nbsp;Ya Zeng ,&nbsp;Qinbo Qin ,&nbsp;Chang Wu ,&nbsp;Yude Wang ,&nbsp;Rurong Zhao ,&nbsp;Min Tao ,&nbsp;Chun Zhang ,&nbsp;Chenchen Tang ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.12.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2022.12.001","url":null,"abstract":"<div><p>Gynogenesis, as a special method of distant hybridization, can be used to accelerate the formation of breeding lines such as fish varieties and populations, and cultivate lines or varieties with higher dominant traits. While, there are few reports on the study of gynogenesis between trans order species in freshwater fish. Even the meat quality comparison between the gynogenetic offspring obtained above and their parents is rarely studied. In order to understand whether the meat quality of naturally gynogenetic blunt snout bream (GBSB) is better than that of its parents (blunt snout bream♀ and Chinese perch♂), we analyzed the texture, composition, antioxidant capacity and nutrients of natural gynogenesis blunt snout bream and its parent muscle. In the muscle of texture characteristics of hardness, springiness, gumminess and chewiness, natural GBSB were better than those of the normal BSB. Muscle fiber size of GBSB is between the maternal and the paternal fish. Compared with the maternal parent, the natural GBSB had higher antioxidant enzyme activities (CAT, SOD and GSH) and higher mineral element (Na, Mg, Ca, P and Zn) contents. In muscle nutrients, the gynogenetic offspring also had more delicious amino acids (Asp, Glu, Gly, Ala, Tyr and Phe) and fatty acids than the maternal parents. Collectively, through distant hybridization between common BSB and Chinese perch (CP), a high-quality gynogenetic fish with more nutrition and stronger muscle performance than maternal fish was obtained. The above results not only provide evidence for the hybridization origin of gynogenetic fish, but also provide new ideas for the innovation of new species.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 4","pages":"Pages 149-155"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000394/pdfft?md5=69d4b0a881af6bc8736e2d0dcc804e02&pid=1-s2.0-S2667071222000394-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137281620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Androgen receptor α peak expression in retina rather than gonad of Hainan medaka, Oryzias curvinotus","authors":"Zhongduo Wang ,&nbsp;Aiping Deng ,&nbsp;Jiahui Huang ,&nbsp;Shunkai Huang ,&nbsp;Gyamfua Afriyie ,&nbsp;Zhongdian Dong ,&nbsp;Yusong Guo","doi":"10.1016/j.repbre.2022.07.001","DOIUrl":"10.1016/j.repbre.2022.07.001","url":null,"abstract":"<div><p>Androgen is a member of the sex steroid hormones family which plays an important role in the development and maturation of the vertebrate reproductive system. The physiological function of androgens is mainly mediated by the androgen receptor (AR). <em>Oryzias curvinotus</em> is a small oviparous fish and a potential model like its sister specie, <em>Oryzias latipes</em>. In this study, two subtypes (<em>α</em> and <em>β</em>) of androgen receptors (<em>ar</em>) genes of <em>O. curvinotus</em> were successfully cloned. Phylogenetic analysis with amino acids showed that <em>arα</em> originates later and differentiates more than <em>arβ</em>. The result of RT-qPCR showed that there were significant differences in the different development stages and different tissues in the expression of two subtypes of <em>ar</em>. <em>Arα</em> remained highly expressed from the eyed stage to the hatching stage, while <em>arβ</em> was lowly expressed. Also, <em>arα</em> was highly expressed in the eyes, liver, and gonads of adults, while <em>arβ</em> remained highly expressed only in adult ovaries. And the result of <em>in situ</em> hybridization confirmed the high expression of <em>arα</em> on the inner nuclear layer of the retina of adult <em>O. curvinotus.</em> Conclusively, the expression and the localization of <em>arα</em> indicated that it plays an important role in both early embryonic development and the eyes of adult <em>O. curvinotus.</em> Further research to enhance the understanding of the new function and mechanism of androgen receptors in medaka is needed.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 89-94"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000254/pdfft?md5=d537e5f6edd1042996e977e263bcae11&pid=1-s2.0-S2667071222000254-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41458256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new type of hybrid golden pompano “Chenhai No.1” produced by the hybridization of (Trachinotus ovatus ♀ × Trachinotus blochii ♂) ♀ × T. ovatus ♂","authors":"Chang Wu ,&nbsp;Jian Li ,&nbsp;Chunyou Cai ,&nbsp;Qinbo Qin ,&nbsp;Chunren Huang ,&nbsp;Zhennian Chen ,&nbsp;Fangzhou Hu ,&nbsp;Jingjie Hu ,&nbsp;Hai Huang ,&nbsp;Jian Luo ,&nbsp;Liu Cao ,&nbsp;Qian Chen ,&nbsp;Xu Huang ,&nbsp;Chenchen Tang ,&nbsp;Yousen Cai ,&nbsp;Jianshun Cai ,&nbsp;Sichuan Cai ,&nbsp;Huiming Cai ,&nbsp;Yibin Chen ,&nbsp;Yang Yang ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.06.001","DOIUrl":"10.1016/j.repbre.2022.06.001","url":null,"abstract":"<div><p>Distant hybridization is a traditional and effective technology in fish genetic breeding. In this study, we reported a new type of golden pompano (Named as Chenhai No.1) produced by the distant hybridization of (<em>Trachinotus ovatus</em> ♀ ​× ​<em>T. blochii</em> ♂) ♀ ​× ​<em>T. ovatus</em> ♂. The results regarding DNA content, chromosomal number, and morphological traits showed that “Chenhai No.1” was a diploid hybrid with 48 chromosomes, and presented a hybrid appearance compared with those in its parents. Meanwhile, all detected “Chenhai No.1” including males and females showed the normal gonadal structure and could produce offspring by self-crossing at 3 years old. In addition, the growth rate of “Chenhai No.1” was 35.52% faster than <em>T. ovatus</em>. In conclusion, the fertile “Chenhai No.1” with faster growth rate provided a superior germplasm resource, which will further promote the industrial development of golden pompano.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 78-82"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000163/pdfft?md5=da1920ec44fe36dff72b5c3204c0c547&pid=1-s2.0-S2667071222000163-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43742225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous paternal mitochondria rescue hybrid incompatibility and the destiny of exogenous mitochondria","authors":"Ming Wen,&nbsp;Yuxin Zhang,&nbsp;Siyu Wang,&nbsp;Qian Li,&nbsp;Liangyue Peng,&nbsp;Qilin Li,&nbsp;Xinjiang Hu,&nbsp;Yuling Zhao,&nbsp;QinBo Qin,&nbsp;Min Tao,&nbsp;Chun Zhang,&nbsp;Kaikun Luo,&nbsp;Rurong Zhao,&nbsp;Shi Wang,&nbsp;Fangzhou Hu,&nbsp;Qingfeng Liu,&nbsp;Yude Wang,&nbsp;Chenchen Tang,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.06.003","DOIUrl":"10.1016/j.repbre.2022.06.003","url":null,"abstract":"<div><p>The mitochondria of most organisms follow strict maternal inheritance, and the mechanism of elimination of paternal mitochondria is unclear. Our previous studies showed that the paternal mtDNA presented in the embryo of hybrid <em>Megalobrama Amblycephala</em> (BSB,♀) ​× ​<em>Carassius auratus</em> red var(RCC,♂) (BR) and its reciprocal hybrid (RB), but its expression was quiescent. However, the microinjected mitochondria persisted in the embryo and its mtDNA expressed throughout embryonic development. In addition, the chromosome number of RCC (2n ​= ​100) is much larger than that of BSB (2n ​= ​48). All BR embryos were severely abnormal and ultimately died, while the RB embryos could survive and grow up into adult. It implied that the nuclear-cytoplasm incompatibility may be an important cause of BR abnormality. In this study, exogenous parental mitochondria were microinjected into BR embryos, and it was found that paternal mitochondrial DNA persisted and expressed throughout embryonic development. Meanwhile, the study also found that microinjection of isolated paternal (RCC) mitochondria into BR embryos significantly improved the degree of early embryonic abnormality with some fry swimming normally, comparing to the control embryos. However, injection of maternal (BSB) mitochondria did not improve the development of BR embryos. We safely concluded exogenous mitochondria escape the mechanism of elimination and its DNA persist and express throughout embryonic development. In addition, the expression of paternal mitochondrial genes largely reduces the cyto-nuclear conflict, so that the early BR embryos exhibit less degree of abnormality and enhanced activity.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 83-88"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000187/pdfft?md5=5e5836935cb9b41182589f469bbb2b31&pid=1-s2.0-S2667071222000187-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46854251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid establishment of Oct4: EGFP transgenic zebrafish homozygote through gynogenesis for monitoring the pluripotency during induction of pluripotent stem cells","authors":"Wenting Xu ,&nbsp;Wen Fu ,&nbsp;Mindi Long ,&nbsp;Xiudan Yuan ,&nbsp;Kaiyue Zhao ,&nbsp;Xiaoli Hu ,&nbsp;Jinhui Liu ,&nbsp;Wenbin Liu ,&nbsp;Liangyue Peng ,&nbsp;Yamei Xiao","doi":"10.1016/j.repbre.2022.08.001","DOIUrl":"10.1016/j.repbre.2022.08.001","url":null,"abstract":"<div><p>Oct4 was one of the important markers of cellular pluripotency. In this study, by Tol2 transgenic technology, the recombinant plasmids composed of <em>Oct4</em> promoter DNA and enhanced green fluorescent protein (EGFP) gene are microinjected into zebrafish 1-cell stage embryos, and obtain the EGFP positive (EGFP⁺) transgenic fish. To generation homozygous transgenic fish, the eggs of EGFP⁺ F0 were activated by ultraviolet inactivated sperm of crucian carp, and then inhibited their first cleavage. The homozygous <em>Oct4</em>-EGFP⁺ zebrafish are successfully obtained in F1 generation. After chemical reprogramming, the clones derived from caudal fin fibroblasts of homozygous <em>Oct4</em>-EGFP⁺ zebrafish are observed green fluorescence. These results suggest that gynogenetic technology provides new strategies for the rapid preparation of homozygous transgenic fish.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 106-111"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000278/pdfft?md5=3eb28c6171a77697a30404b534deed40&pid=1-s2.0-S2667071222000278-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45466267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}