Reproduction and breeding最新文献_第9页

Evolutionarily conserved IL-20 enhances the response of JAK1/STAT3 axis in Carassius cuvieri × Carassius auratus red var 进化保守的IL-20增强鲫鱼×鲫鱼红细胞JAK1/STAT3轴的反应

Reproduction and breeding Pub Date : 2023-06-01 DOI: 10.1016/j.repbre.2023.06.002

Qiongyao Zeng , Niewen Hu , Zhengwei Li, Ye Yang, Zejun Zhou

{"title":"Evolutionarily conserved IL-20 enhances the response of JAK1/STAT3 axis in Carassius cuvieri × Carassius auratus red var","authors":"Qiongyao Zeng , Niewen Hu , Zhengwei Li, Ye Yang, Zejun Zhou","doi":"10.1016/j.repbre.2023.06.002","DOIUrl":"10.1016/j.repbre.2023.06.002","url":null,"abstract":"<div><p>The IL-20 cytokine family, which includes only IL-20, IL-22 and IL-26 in teleost, has a wide range of biological functions and is essential for epithelial integrity and innate host defense. IL-22 and IL-26 are the most intensively studied cytokine within this family; however, the immunological function of IL-20 in fish is essentially unknown. In this study, we examined the expression and function of an IL-20 homologue (WR-IL20) from White crucian carp (<em>Carassius cuvieri</em>, WCC, female) × Red crucian carp (<em>Carassius auratus</em> red var., RCC, male). WR-IL20 and its parents, WCC-IL20 and RCC-IL20, were both composed of 177 residues with high identities among them. Additionally, gene structure, functional domain, tertiary structure, multiple sequence alignment, and phylogeny analysis showed a conserved status between WR-IL20, WCC-IL20 and RCC-IL20. WR-IL20, WCC-IL20 and RCC-IL20 were mainly expressed in the head kidney and were upregulated by <em>Aeromonas hydrophila</em> infection <em>in vivo</em>. Furthermore, recombinant WR-IL20 protein (rWR-IL20) induced increased transcript levels of <em>c-Myc</em>, <em>cyclin D</em>, <em>Bcl-2</em>, <em>JAK1</em>, <em>TYK2</em> and <em>STAT3</em> in head kidney lymphocytes <em>in vitro</em>. These results provide a new perspective for understanding the origin, evolution and function of IL-20 in teleost.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 72-81"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46841284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Role of the dimerization domain of black carp STING during the antiviral innate immunity 黑鱼STING二聚体结构域在抗病毒先天免疫中的作用

Reproduction and breeding Pub Date : 2023-06-01 DOI: 10.1016/j.repbre.2023.05.002

Jun Yan , Guoxia Qiao , Haiyan Chang , Hui Wu , Meiling Liu , Jiagang Tu , Yongan Zhang , Hao Feng

{"title":"Role of the dimerization domain of black carp STING during the antiviral innate immunity","authors":"Jun Yan , Guoxia Qiao , Haiyan Chang , Hui Wu , Meiling Liu , Jiagang Tu , Yongan Zhang , Hao Feng","doi":"10.1016/j.repbre.2023.05.002","DOIUrl":"10.1016/j.repbre.2023.05.002","url":null,"abstract":"<div><p>Stimulator of interferon gene (STING) functions importantly as antiviral adaptor protein during the innate immune activation. The role of the dimerization domain (DD) of STING remains obscure although other domains of this molecule have been studied extensively. To clarify the mechanism of black carp STING (bcSTING) in the innate immunity, bcSTING-ΔDD (bcSTING without DD) and bcSTING-ΔCTT (bcSTING without CTT domain) were constructed and analyzed in this manuscript. The reporter assays revealed that the induced transcription of IFN promoters mediated by bcSTING-ΔDD were much higher than that of the wild type bcSTING; however, bcSTING-ΔCTT almost lost the activities to trigger the interferon (IFN) promoters transcription. The mRNA transcriptional levels of IFN and interferon stimulated genes (ISGs) in EPC cells expressing bcSTING-ΔDD were obviously higher than those of EPC cells expressing wild type bcSTING; however, the transcriptional levels of the above cytokines of EPC cells expressing bcSTING-ΔCTT were basically the same as those of control cells. EPC cells overexpressing bcSTING-DD showed stronger antiviral activity than those overexpressing wild-type bcSTING. Furthermore, the co-immunoprecipitation assay identified the self-interaction between bcSTING-ΔDD molecules. And it was interesting that the affinity between bcSTING-ΔDD and bcTBK1 was obviously stronger than that between bcTBK1 and wild-type bcSTING. Thus, our data suggests that DD of black carp STING plays a negative regulatory role in STING-mediated antiviral immunity, which provides a new perspective for further researching the function of fish STING in the innate immunity.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 59-65"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48650901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximate compositions evaluation, histology and transcriptome analysis revealed the effects of formulated diets on muscle quality in Micropterus salmoides 近似成分评估、组织学和转录组分析揭示了配方日粮对细鳍金枪鱼肌肉质量的影响

Reproduction and breeding Pub Date : 2023-06-01 DOI: 10.1016/j.repbre.2023.04.002

Zaixuan Zhong , Jiajia Fan , Huanhuan Su , Huaping Zhu , Dongmei Ma

{"title":"Proximate compositions evaluation, histology and transcriptome analysis revealed the effects of formulated diets on muscle quality in Micropterus salmoides","authors":"Zaixuan Zhong , Jiajia Fan , Huanhuan Su , Huaping Zhu , Dongmei Ma","doi":"10.1016/j.repbre.2023.04.002","DOIUrl":"10.1016/j.repbre.2023.04.002","url":null,"abstract":"<div><p>With the gradually upgrade of formulated feeds, the largemouth bass <em>Micropterus salmoides</em> has been domesticated to adapt to formulated diets to reduce the use of bait fish and cut cost. However, whether the formulated diets influence the nutritional content and muscle quality of largemouth bass and related mechanisms remains unclear. In this study, we evaluated the effects of forage fish and formulated diets on growth performance, proximate compositions, muscle histology and fiber character. Largemouth bass with initial weights of 39.37 ± 1.37g and 39.45 ± 1.40g were fed with formulated diets and forage fish for 8 months, respectively. The results showed that formulated diets group (FDG) exhibited higher levels of protein content and free amino acids compared with forage fish group (FFG). Interestingly, the diameter of muscle fiber was larger in FDG than in FFG through microsection observation. A comparative analysis of transcriptome was constructed for muscle tissues of FDG and FFG, respectively. Compared with FFG, 2186 differentially expressed genes (DEGs) were identified in FDG, including 1915 upregulated genes and 271 downregulated genes. Gene ontology (GO) analyses revealed that DEGs were significantly enriched in GO terms regarding mitochondrial metabolism, muscle growth and development. KEGG enrichment indicated that DEGs were involved in the amino acid metabolism. Our results indicated that replacing forage fish with formulated diets affected the muscle quality and fiber character of largemouth bass, which could provide a basis for the regulation of nutrition and accurate selection for meat traits in largemouth bass.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 50-58"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46654168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Full-length transcriptome reveals rapid genetic changes in triploid hybrid grass carp derived from female grass carp × male topmouth culter 全长转录组揭示了由雌性草鱼×雄性顶嘴培育的三倍体杂交草鱼的快速遗传变化

Reproduction and breeding Pub Date : 2023-06-01 DOI: 10.1016/j.repbre.2023.06.001

Xu Huang , Jiawang Huang , Lang Qin , Yali Long , Chang Wu, Kaijun Gong, Qingwen Xiao, Qinbo Qin

{"title":"Full-length transcriptome reveals rapid genetic changes in triploid hybrid grass carp derived from female grass carp × male topmouth culter","authors":"Xu Huang , Jiawang Huang , Lang Qin , Yali Long , Chang Wu, Kaijun Gong, Qingwen Xiao, Qinbo Qin","doi":"10.1016/j.repbre.2023.06.001","DOIUrl":"10.1016/j.repbre.2023.06.001","url":null,"abstract":"<div><p>Distant hybridization refers to the combination of different genomes from different species and further leads to genetic structure changes in hybrids. For next-generation sequencing limitations, the genetic changes with respect to hybrids or polyploids cannot be fully explained. Here, we obtained 117,526 full-length isoforms from a triploid hybrid grass carp (3nGT, 3n = 72) by the crossing of female grass carp (GC, 2n = 48) and male topmouth culter (TC, 2n = 48) using single-molecule long reads sequencing technology. Further, the gene fusion, alternative splicing (AS), transcription of homologous genes, and novel genes were analyzed. The results showed that 1563 fusion genes were detected in 3nGT including 872 heterogenous fusion genes derived from GC and TC genomes. Meanwhile, 175,644 AS events were identified and divided into five models, of which IntronR was the dominant model. In addition, 8964 novel genes were discovered, of which 2802 novel genes were annotated. Finally, scans results of <em>Hox</em> gene family revealed that both GC and TC <em>Hox</em> genes were transcribed in 3nGT, but presented asymmetric transcription patterns with a bias towards the maternal parent GC genome. This study not only provides a rich resource of transcript isoforms for the superior triploid grass carp, but also provides an important insight into rapid genetic changes in hybrid fish.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 2","pages":"Pages 82-87"},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43923711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic parameter estimates for litter traits in Jakhrana breed of Indian goat 印度山羊Jakhrana品种产羔性状的遗传参数估计

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2023.03.001

Yogesh C. Bangar, Ankit Magotra, A.S. Yadav

{"title":"Genetic parameter estimates for litter traits in Jakhrana breed of Indian goat","authors":"Yogesh C. Bangar, Ankit Magotra, A.S. Yadav","doi":"10.1016/j.repbre.2023.03.001","DOIUrl":"10.1016/j.repbre.2023.03.001","url":null,"abstract":"<div><p>The litter size and litter weight are the most important indicators of growth production system in goats as they are directly related to the survival and growth of the kids. The present attempt was made to estimate genetic parameters of litter traits such as litter size at birth (LSB), litter size at weaning (LSW), litter weight at birth (LWB) and litter weight at weaning (LWW) of Jakhrana breed of Indian goat. These traits were subjected to study variation due to direct additive genetic effects by adjusting non-genetic factors under linear mixed model. The data of litter records of 101 Jakhrana goats including pedigree information were processed and the results showed that the least-square means for LSB, LSW, LWB and LWW were 1.20 ± 0.05, 1.19 ± 0.05, 3.08 ± 0.11 and 12.11 ± 0.49, respectively. The direct heritability estimates for LSB, LSW, LWB and LWW were 0.17, 0.01, 0.001 and 0.10, respectively. The genetic (0.70–0.97) and phenotypic (0.61–0.92) correlation estimates among litter traits were high and positive. This study indicated that the additive variation among the early reproductive traits was low in range which does not provide a warrant for the improvement through selection. However, the evaluation of these traits has provided the current state of litter performance of Jakhrana goat which may be upgraded by managerial practices specially care and feeding of pregnant goats.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 35-37"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43644067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gynogenetic Cirrhinus mrigala produced using irradiated sperm of Cyprinus carpio exhibit better cold tolerance 鲤鱼辐照精子产生的Gynogenetic Cirrhinus mrigala具有较好的抗寒性

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2023.01.001

Wuhui Li , Zexun Zhou , Xuelei Tian , Hongqing Li , Jisen Su , Qilong Liu , Ping Wu , Shi Wang , Jie Hu , Zhongyuan Shen , Lei Zeng , Min Tao , Chun Zhang , Qinbo Qin , Shaojun Liu

{"title":"Gynogenetic Cirrhinus mrigala produced using irradiated sperm of Cyprinus carpio exhibit better cold tolerance","authors":"Wuhui Li , Zexun Zhou , Xuelei Tian , Hongqing Li , Jisen Su , Qilong Liu , Ping Wu , Shi Wang , Jie Hu , Zhongyuan Shen , Lei Zeng , Min Tao , Chun Zhang , Qinbo Qin , Shaojun Liu","doi":"10.1016/j.repbre.2023.01.001","DOIUrl":"10.1016/j.repbre.2023.01.001","url":null,"abstract":"<div><p>Artificial gynogenesis is an important method for accelerating the selective breeding of varieties and populations. In this study, ultraviolet-irradiated sperm of Common carp (<em>Cyprinus carpio</em>) was used to activate the mature eggs of mrigal carp (<em>Cirrhinus mrigala</em>, MC), after cold shock at 4–8 °C for 16 min to double the chromosomes, a population of gynogenetic mrigal carp (GMC) was obtained. The fertilization rate, hatching rate and survival rate of GMC exceeded 62.2%, 6.3% and 2.9%, respectively. Comparative analyses of morphological traits, DNA content, chromosome numbers and 5S rDNA sequences showed no significant differences between MC and GMC. However, microsatellite (MFW1, CCA15) analysis revealed that paternal DNA fragments were inserted and a parent DNA fragment was deleted in the GMC. The GMC population survived the natural winter for more than 50 days with a temperature below 10 °C, while all the normal MC individuals perished. In addition, lower critical thermal minimum (CTMin) and lethal temperature (T<sub>LD50</sub>) values were detected in GMC than that in MC. Our results indicated that the artificial gynogenesis method improved the cold tolerance of mrigal carp. The results of this study are of great significance for fish breeding and application.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 8-16"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45000080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Air-assisted liquid-liquid microextraction and enantioselective gas chromatography-tandem mass spectrometry quantification of methyl jasmonate stereoisomers in tea (Camellia sinensis L.) 空气辅助液-液微萃取-对映选择性气相色谱-串联质谱法测定茶叶中茉莉酸甲酯立体异构体

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2023.01.002

Zhoufei Luo , Xianxin Zhu , Hui Li , Yu Jian , Wei Li , Haiou Li , Chao Huang , Ruozhong Wang , Langtao Xiao

{"title":"Air-assisted liquid-liquid microextraction and enantioselective gas chromatography-tandem mass spectrometry quantification of methyl jasmonate stereoisomers in tea (Camellia sinensis L.)","authors":"Zhoufei Luo , Xianxin Zhu , Hui Li , Yu Jian , Wei Li , Haiou Li , Chao Huang , Ruozhong Wang , Langtao Xiao","doi":"10.1016/j.repbre.2023.01.002","DOIUrl":"10.1016/j.repbre.2023.01.002","url":null,"abstract":"<div><p>Different stereoisomers of phytohormones may have different roles in plants while the quantification for stereoisomers still remains a challenge. The chiral separation method for methyl jasmonate (MeJA) in tea (<em>Camellia sinensis</em> L.) was developed by air-assisted liquid-liquid microextraction (AALLME) with enantioselective gas chromatography-mass spectrometry (Es-GC-MS/MS). The Plackett-Burman factorial design was introduced to optimize the multiple parameters of AALLME. Sensitive Es-GC-MS/MS enabled the quantification of the MeJA stereoisomers, which were separated from each other with resolutions of 3.5, 2.7, and 2.5. The linearities were <em>R</em><sup>2</sup> > 0.9971, the limits of detection were 0.08–0.12 ng/mL, the limits of quantification (LOD) were 0.28–0.39 ng/mL, and the recovery range was 80.60–107.03%. The method was applied to analyze the MeJA stereoisomer profiles in tea plants after geometrid feeding and mechanical wounding. (+)-MeJA appeared to be the main bio-active monomer that responded to injury stress. (+)-MeJA similarly increased in tea shoots at only 30 s after geometrid attack or mechanical wounding. The proposed method is superior as air-assisted liquid-liquid microextraction could induce the loss of volatile MeJA in a much shorter time, compared to the conventional enrichment procedure. A novel assay protocol for relative quantification of MeJA stereoisomers in plants responding to different injuries was applied.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 17-25"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45777310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The formation and study of allogynogenesis Hemibarbus maculatus Bleeker 同种异体斑半倒刺的形成与研究

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2022.12.002

Yude Wang , An'min Liao , Chen Geng , Huifang Tan , Rurong Zhao , Shi Wang , Ming Wen , Kaikun Luo , Qinbo Qin , Chun Zhang , Min Tao , Shaojun Liu

{"title":"The formation and study of allogynogenesis Hemibarbus maculatus Bleeker","authors":"Yude Wang , An'min Liao , Chen Geng , Huifang Tan , Rurong Zhao , Shi Wang , Ming Wen , Kaikun Luo , Qinbo Qin , Chun Zhang , Min Tao , Shaojun Liu","doi":"10.1016/j.repbre.2022.12.002","DOIUrl":"10.1016/j.repbre.2022.12.002","url":null,"abstract":"<div><p><em>Hemibarbus maculatus</em> Bleeker (HM) is an economically important fish that is highly valued by consumers. Gynogenesis is an important breeding method in aquaculture that has been widely applied to many fish species. In this study, we adopted an improved method of gynogenesis that proceeds as follows: first, inactivated koi carp sperm were used to stimulate eggs of HM, and then the UV-inactivated koi carp (KOC) sperm and HM eggs were mixed and stirred for 20–30 s to activate the eggs. The activated eggs were placed in yellow mud at 0 °C for 20–22 min for cold shock treatment, and then placed in water at 4–6 °C, 8–10 °C, and 15–18 °C in order, each for 1–2 min. After incubation at 22–25 °C, gynogenetic <em>Hemibarbus maculatus</em> Bleeker (GHM) were obtained. Morphological measurements and blood cell comparisons showed that there were no significant differences between GHM and HM. At the molecular level, comparative analysis of microsatellites DNA indicated that one fragment of GHM came from the KOC. This study provides the first evidence that DNA fragments from male parents exist in GHM at the molecular level. This discovery has important implications for fish genetic breeding.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 1-7"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43255759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Protection and utilization status of Parabramis and Megalobrama germplasm resources Parabramis和Megalobama种质资源的保护与利用现状

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2023.01.003

Dingbin Gong , Xueyan Wang , Jingyun Yang , Jiayi Liang , Min Tao , Fangzhou Hu , Shi Wang , Zheng Liu , Chenchen Tang , Kaikun Luo , Chun Zhang , Ming Ma , Yuequn Wang , Shaojun Liu

{"title":"Protection and utilization status of Parabramis and Megalobrama germplasm resources","authors":"Dingbin Gong , Xueyan Wang , Jingyun Yang , Jiayi Liang , Min Tao , Fangzhou Hu , Shi Wang , Zheng Liu , Chenchen Tang , Kaikun Luo , Chun Zhang , Ming Ma , Yuequn Wang , Shaojun Liu","doi":"10.1016/j.repbre.2023.01.003","DOIUrl":"10.1016/j.repbre.2023.01.003","url":null,"abstract":"<div><p>The genera <em>Parabramis</em> and <em>Megalobrama</em> are important freshwater aquaculture economic fish, and the excavation and utilization of excellent germplasm resources are pivotal in ensuring the supply of high-quality aquatic protein in China. However, as a consequence of fast domestication, overfishing and water pollution, the germplasm resources of <em>Parabramis</em> and <em>Megalobrama</em> are under threat of recession. These germplasm resources serve as an indispensable material basis for the healthy and sustainable development of the <em>Parabramis</em> and <em>Megalobrama</em> breeding industries. It is of great strategic significance to systematically collect, preserve, identify, evaluate, protect and utilize <em>Parabramis</em> and <em>Megalobrama</em> germplasm resources. This paper reviews the major results of studies on the morphology, cytogenetics, and molecular population genetics of the genera <em>Parabramis</em> and <em>Megalobrama</em>, the progress in their genetic improvement through hybridization, selective breeding and gynogenesis techniques, and the current protection measures for <em>Parabramis</em> and <em>Megalobrama</em> germplasm resources. Then, some suggestions for the protection of these germplasm resources and improved variety creation are provided. It is expected to offer basic resources for the sustainable development of the <em>Parabramis</em> and <em>Megalobrama</em> seed industries.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 26-34"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48147649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The infection of murine gammaherpesvirus-68 delays the early embryonic development of zebrafish 小鼠γ疱疹病毒68的感染可延缓斑马鱼早期胚胎发育

Reproduction and breeding Pub Date : 2023-03-01 DOI: 10.1016/j.repbre.2023.03.002

Jun Yan , Mengdie Chi , Jun Xiao , Meiling Liu , Hao Feng

{"title":"The infection of murine gammaherpesvirus-68 delays the early embryonic development of zebrafish","authors":"Jun Yan , Mengdie Chi , Jun Xiao , Meiling Liu , Hao Feng","doi":"10.1016/j.repbre.2023.03.002","DOIUrl":"10.1016/j.repbre.2023.03.002","url":null,"abstract":"<div><p>Zebrafish is a model animal for aquaculture as well as bio-medicine. Murine gamma-herpesvirus 68 (MHV68) is widely regarded as a model for the study of human gamma herpesviruses such as Kaposi's sarcoma-associated herpesvirus (KSHV). In this study, the infection of MHV68 on zebrafish was investigated through incubating fertilized zebrafish eggs with MHV68. The development of zebrafish delayed or stopped at early stage separately when the zebrafish eggs were incubated with MHV68 at different dose for 2 h. The lytic gene of MHV68, including ORF65, RTA and ORF57, were detected in the genome of larvae hatched from the infected eggs. The transcription of the latent gene LANA but not of these lytic genes was detected by RT-PCR. The mRNA level of growth factor TGF-β1, but not of FGF3, was obviously decreased in the larvae from the MHV68 treated eggs. The mRNA levels of cytokines, such as IFNφ3, ISG15 and TNFα, varied post MHV68 infection; however, the transcription of the cytosolic DNA sensors, including DDX41, DHX9 and cGAS, did not change significantly. Moreover, the data generated in zebrafish cell line demonstrated that MHV68 could infect ZF4 cells. All the data support the conclusion that MHV68 could infect zebrafish embryo in the egg envelope and go into latent infection, which will delay the development of the embryos through some unknown mechanism.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"3 1","pages":"Pages 38-44"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48099040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0