Reproduction and breeding最新文献

{"title":"Androgen receptor α peak expression in retina rather than gonad of Hainan medaka, Oryzias curvinotus","authors":"Zhongduo Wang ,&nbsp;Aiping Deng ,&nbsp;Jiahui Huang ,&nbsp;Shunkai Huang ,&nbsp;Gyamfua Afriyie ,&nbsp;Zhongdian Dong ,&nbsp;Yusong Guo","doi":"10.1016/j.repbre.2022.07.001","DOIUrl":"10.1016/j.repbre.2022.07.001","url":null,"abstract":"<div><p>Androgen is a member of the sex steroid hormones family which plays an important role in the development and maturation of the vertebrate reproductive system. The physiological function of androgens is mainly mediated by the androgen receptor (AR). <em>Oryzias curvinotus</em> is a small oviparous fish and a potential model like its sister specie, <em>Oryzias latipes</em>. In this study, two subtypes (<em>α</em> and <em>β</em>) of androgen receptors (<em>ar</em>) genes of <em>O. curvinotus</em> were successfully cloned. Phylogenetic analysis with amino acids showed that <em>arα</em> originates later and differentiates more than <em>arβ</em>. The result of RT-qPCR showed that there were significant differences in the different development stages and different tissues in the expression of two subtypes of <em>ar</em>. <em>Arα</em> remained highly expressed from the eyed stage to the hatching stage, while <em>arβ</em> was lowly expressed. Also, <em>arα</em> was highly expressed in the eyes, liver, and gonads of adults, while <em>arβ</em> remained highly expressed only in adult ovaries. And the result of <em>in situ</em> hybridization confirmed the high expression of <em>arα</em> on the inner nuclear layer of the retina of adult <em>O. curvinotus.</em> Conclusively, the expression and the localization of <em>arα</em> indicated that it plays an important role in both early embryonic development and the eyes of adult <em>O. curvinotus.</em> Further research to enhance the understanding of the new function and mechanism of androgen receptors in medaka is needed.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 89-94"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000254/pdfft?md5=d537e5f6edd1042996e977e263bcae11&pid=1-s2.0-S2667071222000254-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41458256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new type of hybrid golden pompano “Chenhai No.1” produced by the hybridization of (Trachinotus ovatus ♀ × Trachinotus blochii ♂) ♀ × T. ovatus ♂","authors":"Chang Wu ,&nbsp;Jian Li ,&nbsp;Chunyou Cai ,&nbsp;Qinbo Qin ,&nbsp;Chunren Huang ,&nbsp;Zhennian Chen ,&nbsp;Fangzhou Hu ,&nbsp;Jingjie Hu ,&nbsp;Hai Huang ,&nbsp;Jian Luo ,&nbsp;Liu Cao ,&nbsp;Qian Chen ,&nbsp;Xu Huang ,&nbsp;Chenchen Tang ,&nbsp;Yousen Cai ,&nbsp;Jianshun Cai ,&nbsp;Sichuan Cai ,&nbsp;Huiming Cai ,&nbsp;Yibin Chen ,&nbsp;Yang Yang ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.06.001","DOIUrl":"10.1016/j.repbre.2022.06.001","url":null,"abstract":"<div><p>Distant hybridization is a traditional and effective technology in fish genetic breeding. In this study, we reported a new type of golden pompano (Named as Chenhai No.1) produced by the distant hybridization of (<em>Trachinotus ovatus</em> ♀ ​× ​<em>T. blochii</em> ♂) ♀ ​× ​<em>T. ovatus</em> ♂. The results regarding DNA content, chromosomal number, and morphological traits showed that “Chenhai No.1” was a diploid hybrid with 48 chromosomes, and presented a hybrid appearance compared with those in its parents. Meanwhile, all detected “Chenhai No.1” including males and females showed the normal gonadal structure and could produce offspring by self-crossing at 3 years old. In addition, the growth rate of “Chenhai No.1” was 35.52% faster than <em>T. ovatus</em>. In conclusion, the fertile “Chenhai No.1” with faster growth rate provided a superior germplasm resource, which will further promote the industrial development of golden pompano.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 78-82"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000163/pdfft?md5=da1920ec44fe36dff72b5c3204c0c547&pid=1-s2.0-S2667071222000163-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43742225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous paternal mitochondria rescue hybrid incompatibility and the destiny of exogenous mitochondria","authors":"Ming Wen,&nbsp;Yuxin Zhang,&nbsp;Siyu Wang,&nbsp;Qian Li,&nbsp;Liangyue Peng,&nbsp;Qilin Li,&nbsp;Xinjiang Hu,&nbsp;Yuling Zhao,&nbsp;QinBo Qin,&nbsp;Min Tao,&nbsp;Chun Zhang,&nbsp;Kaikun Luo,&nbsp;Rurong Zhao,&nbsp;Shi Wang,&nbsp;Fangzhou Hu,&nbsp;Qingfeng Liu,&nbsp;Yude Wang,&nbsp;Chenchen Tang,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.06.003","DOIUrl":"10.1016/j.repbre.2022.06.003","url":null,"abstract":"<div><p>The mitochondria of most organisms follow strict maternal inheritance, and the mechanism of elimination of paternal mitochondria is unclear. Our previous studies showed that the paternal mtDNA presented in the embryo of hybrid <em>Megalobrama Amblycephala</em> (BSB,♀) ​× ​<em>Carassius auratus</em> red var(RCC,♂) (BR) and its reciprocal hybrid (RB), but its expression was quiescent. However, the microinjected mitochondria persisted in the embryo and its mtDNA expressed throughout embryonic development. In addition, the chromosome number of RCC (2n ​= ​100) is much larger than that of BSB (2n ​= ​48). All BR embryos were severely abnormal and ultimately died, while the RB embryos could survive and grow up into adult. It implied that the nuclear-cytoplasm incompatibility may be an important cause of BR abnormality. In this study, exogenous parental mitochondria were microinjected into BR embryos, and it was found that paternal mitochondrial DNA persisted and expressed throughout embryonic development. Meanwhile, the study also found that microinjection of isolated paternal (RCC) mitochondria into BR embryos significantly improved the degree of early embryonic abnormality with some fry swimming normally, comparing to the control embryos. However, injection of maternal (BSB) mitochondria did not improve the development of BR embryos. We safely concluded exogenous mitochondria escape the mechanism of elimination and its DNA persist and express throughout embryonic development. In addition, the expression of paternal mitochondrial genes largely reduces the cyto-nuclear conflict, so that the early BR embryos exhibit less degree of abnormality and enhanced activity.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 83-88"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000187/pdfft?md5=5e5836935cb9b41182589f469bbb2b31&pid=1-s2.0-S2667071222000187-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46854251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid establishment of Oct4: EGFP transgenic zebrafish homozygote through gynogenesis for monitoring the pluripotency during induction of pluripotent stem cells","authors":"Wenting Xu ,&nbsp;Wen Fu ,&nbsp;Mindi Long ,&nbsp;Xiudan Yuan ,&nbsp;Kaiyue Zhao ,&nbsp;Xiaoli Hu ,&nbsp;Jinhui Liu ,&nbsp;Wenbin Liu ,&nbsp;Liangyue Peng ,&nbsp;Yamei Xiao","doi":"10.1016/j.repbre.2022.08.001","DOIUrl":"10.1016/j.repbre.2022.08.001","url":null,"abstract":"<div><p>Oct4 was one of the important markers of cellular pluripotency. In this study, by Tol2 transgenic technology, the recombinant plasmids composed of <em>Oct4</em> promoter DNA and enhanced green fluorescent protein (EGFP) gene are microinjected into zebrafish 1-cell stage embryos, and obtain the EGFP positive (EGFP⁺) transgenic fish. To generation homozygous transgenic fish, the eggs of EGFP⁺ F0 were activated by ultraviolet inactivated sperm of crucian carp, and then inhibited their first cleavage. The homozygous <em>Oct4</em>-EGFP⁺ zebrafish are successfully obtained in F1 generation. After chemical reprogramming, the clones derived from caudal fin fibroblasts of homozygous <em>Oct4</em>-EGFP⁺ zebrafish are observed green fluorescence. These results suggest that gynogenetic technology provides new strategies for the rapid preparation of homozygous transgenic fish.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 106-111"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000278/pdfft?md5=3eb28c6171a77697a30404b534deed40&pid=1-s2.0-S2667071222000278-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45466267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The isolation of a novel Streptomyces termitum and identification its active substance against fish pathogens","authors":"Yanan Peng ,&nbsp;Ximiao Lai ,&nbsp;Pan Wang ,&nbsp;Wensu Long ,&nbsp;Feng Zhai ,&nbsp;Shengbiao Hu ,&nbsp;Yibo Hu ,&nbsp;Jun Cui ,&nbsp;Weitao Huang ,&nbsp;Ziquan Yu ,&nbsp;Shijia Yang ,&nbsp;Ganfeng Yi ,&nbsp;Liqiu Xia","doi":"10.1016/j.repbre.2022.07.002","DOIUrl":"10.1016/j.repbre.2022.07.002","url":null,"abstract":"<div><p>The emerging outbreak of bacterial diseases poses a major threat to the aquaculture industry, and the abuse of antibiotics leads to the emergence of drug-resistant pathogens; hence, the development of antibacterial agents from natural resources to control and manage fish bacterial diseases has become a promising strategy. In this study, seven potential <em>Streptomyces</em> spp. with antimicrobial activity against fish pathogenic bacteria were isolated from the soils of different regions in China. <em>Streptomyces termitum</em> N-15, which showed the strongest and most stable antibacterial activity, was selected for the fish disease control experiment. Then, we investigated the effect of a basal diet and N-15 supplementation (10⁸ ​CFU/mg diet) on growth performance, blood immune parameters (ACP and AKP), and the expression of immune genes. The results demonstrated that the ACP and AKP activities of serum were significantly increased in the N-15-supplemented group. Moreover, N-15 upregulated the expression level of Toll-like receptor 4 (<em>TLR-4</em>) in the kidney, liver, spleen and head kidney. The challenge test showed that supplementation with N-15 could significantly improve the survival rates of grass carp infected with <em>A. veronii</em>. Chalcomycin A was purified from <em>Streptomyces termitum</em> N-15 and found to have antimicrobial activity against fish pathogenic bacteria for the first time. Furthermore, the complete genome of <em>S. termitum</em> N-15 was found to consist of an 8,887,222 bp circular chromosome with 72.89% GC content and a plasmid genome, including 7744 protein-coding sequences (CDSs). The genome of <em>S. termitum</em> N-15 contained gene clusters for the synthesis of multiple secondary metabolites, including melingomycin, sulforaphane, oromycin, chlortetracycline, isomycin, and primycin, by antiSMASH online software. Gene cluster 1.5 revealed a chalcomycin A biosynthesis gene cluster (chmGⅠ, chmGⅡ, chmGⅢ, chmGⅣ, chmGⅤ). In summary, <em>S. termitum</em> N-15 and its secondary metabolites are expected to be developed into inoculants for promoting fish health in aquacultural systems.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 95-105"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000266/pdfft?md5=48c319ba67e33b5794fa12cbfba139a4&pid=1-s2.0-S2667071222000266-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43209659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of muscle nutrient in two types of hybrid bream and native bream","authors":"Shengnan Li ,&nbsp;Xiangqiong Yang ,&nbsp;Siyu Fan ,&nbsp;Zhifeng Zhou ,&nbsp;Rong Zhou ,&nbsp;Chang Wu ,&nbsp;Dingbin Gong ,&nbsp;Ming Wen ,&nbsp;Yuequn Wang ,&nbsp;Min Tao ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2022.06.002","DOIUrl":"10.1016/j.repbre.2022.06.002","url":null,"abstract":"<div><p>Distant hybridization can integrate parental advantages to produce new varieties, which may also improve the fish meat quality. Fish is considered to be one of the healthiest meats because it can provide rich protein sources and high levels of omega-3 long-chain polyunsaturated fatty acids. Two types of hybrid bream (BBTB and BTBB) were obtained by the two rounds of backcrossing with an established hybrid lineage of blunt snout bream (<em>Megalobrama amblycephala</em>, BSB, ♀) ​× ​topmouth culter (<em>Culter alburnus</em>, TC, ♂). In the present study, the muscle nutrients, including moisture, fat, protein and ash contents, as well as amino acid and fatty acid compositions, in BBTB, BTBB and their parent BSB with the age of four months were investigated and analyzed by biochemical methods. The results showed that the moisture content of BTBB (76.6%) was lower than that of BSB (77.4%) and BBTB (77.3%), while the protein content of BBTB (17.1%) and BTBB (17.6%) was higher than that of BSB (16.3%). Seventeen amino acids were detected in the muscles from three kinds of fish, among which total amino acids, essential amino acids and delicious amino acids in BTBB and BBTB were all to different degrees higher than those of BSB. Further analysis of fatty acids revealed that BTBB and BBTB exhibited higher levels of unsaturated fatty acids (UFAs) content and the sum content of eicosapentaenoic acid (EPA, C20:5 n-3) and docosahexaenoic acid (DHA, C22:6 n-3) in muscle than BSB. Taken together, the results showed the advantages of BBTB and BTBB in nutritional value and taste, which provided theoretical support for their production application.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 3","pages":"Pages 71-77"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000175/pdfft?md5=54b759f87f48bf27d4080ebc41239d05&pid=1-s2.0-S2667071222000175-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42746734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small RNA in plant meiosis and gametogenesis","authors":"Chenjiang You ,&nbsp;Yue Yu ,&nbsp;Yingxiang Wang","doi":"10.1016/j.repbre.2022.05.004","DOIUrl":"10.1016/j.repbre.2022.05.004","url":null,"abstract":"<div><p>Small RNAs are short non-coding nucleotides and regulate various biological processes in eukaryotes. During past decades, numerous studies have demonstrated the biogenesis of small RNAs and their modes of action in regulating gene expression at the transcriptional and post-transcriptional level. Based on the involvement of genes in biogenesis and function, small RNAs can be classified into three categories, miRNA, hc-siRNA, and phasiRNA. With the continuous advances in cell sorting/isolation technology and next-generation sequencing, recent discoveries provided evidence that all three categories play important roles in reproductive development in plants including model plants and crops. This review summarizes the recent findings on small RNAs, their potential targets, and genes involved in small RNA biogenesis in plant reproductive development and mainly focuses on gametogenesis.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 2","pages":"Pages 65-70"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000151/pdfft?md5=aa501a9ce886f28e94e05a34d387d05a&pid=1-s2.0-S2667071222000151-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47934978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duplicated Tyr disruption using CRISPR/Cas9 reveals melanophore formation in Oujiang color common carp (Cyprinus carpio var. color)","authors":"Xidan Xu,&nbsp;Honglin Chen,&nbsp;Biplab Kumar Mandal,&nbsp;Zhouxuan Si,&nbsp;Jun Wang,&nbsp;Chenghui Wang","doi":"10.1016/j.repbre.2022.05.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2022.05.001","url":null,"abstract":"<div><p>Gene duplication plays an important role in the regulation of biological traits. The <em>Tyr</em> inactivation have been confirmed to hinder melanin synthesis, but the research of duplicated <em>Tyr</em> function and evolution in fish, especially in ornamental farm fish, is insufficient. To test whether the duplicated <em>Tyr</em> has effect on black patches formation in Oujiang color common carp (<em>Cyprinus carpio</em> var. <em>color</em>), we obtained duplicated <em>Tyr</em> gene (<em>Tyr1</em> and <em>Tyr2</em>), each of them possesses biased expression in melanocyte-rich skin, indicating existence of function. The various mutants by disrupting <em>Tyr</em> in white-black (WB) types of Oujiang color common carp using the CRISPR/Cas9 tool were generated. The <em>Tyr2</em> mosaic mutant WB exhibited shallow black patches, while simultaneously disrupting two <em>Tyr</em> genes caused different levels of melanin loss in the eyes and on black patches. Most obviously, the complete albino individuals were obtained with non-wild type sequence and distinguished from whole white (WW) in the pigmentation of eyes. The correlation analysis between the gene mutation type and phenotype revealed that duplicated <em>Tyr</em> were co-involved in melanogenesis, where <em>Tyr1</em> was the key factor, and <em>Tyr2</em> might have been playing compensatory roles on the melanin synthesis in Oujiang color common carp. Our study provided insight into regulation mechanism of black patches formation in fish and breeding of special traits in future.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 2","pages":"Pages 37-45"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000126/pdfft?md5=4e01a9707e38a4f4734f9788b70d5f27&pid=1-s2.0-S2667071222000126-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136699282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsFH13, a type I formin, is indispensable for rice morphogenesis","authors":"Anran Ren ,&nbsp;Jiao Zhang ,&nbsp;Zengyu Liu ,&nbsp;Pingzhou Du ,&nbsp;Fengli Zhang ,&nbsp;Haiyun Ren ,&nbsp;Dabing Zhang","doi":"10.1016/j.repbre.2022.05.003","DOIUrl":"10.1016/j.repbre.2022.05.003","url":null,"abstract":"<div><p>Actin binding proteins regulate plant morphogenesis by regulating actin dynamics and formins affect plant morphogenesis by modulating cytoskeleton dynamics. However, the roles of formins in rice morphogenesis remain largely unclear. Here, we report the biochemical and biological functions of formin homolog 13 (OsFH13), one type I formin, in regulating rice morphogenesis. <em>OsFH13</em> was found to be ubiquitously expressed in both vegetative and reproductive organs including roots, stems, leaves, and flowers; while OsFH13 was found to be localized on the plasma membrane. The <em>osfh13</em> mutant displayed pleiotropic phenotypes including reduced plant height, shorter roots, shorter stems, shrank flag leaves, and smaller seeds due to defective cell elongation and expansion. In the absence of OsFH13, actin filaments arrays were found to be unstable both <em>in vivo</em> and in <em>vitro</em>. In addition, OsFH13 was also found to be able to bundle microtubules <em>in vitro</em>. These results demonstrate that OsFH13 is critical to rice morphogenesis via stabilizing actin network, which could be useful to breed rice with desired morphogenesis.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 2","pages":"Pages 46-55"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266707122200014X/pdfft?md5=2b660d13c4fb0f2b650093104255e4a4&pid=1-s2.0-S266707122200014X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44789601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture conditions for in vitro maturation of oocytes – A review","authors":"Mohammad Bahrami ,&nbsp;Pauline A. Cottee","doi":"10.1016/j.repbre.2022.04.001","DOIUrl":"10.1016/j.repbre.2022.04.001","url":null,"abstract":"<div><p>The maturation of oocytes external to the body is referred to IVM and is utilised by many different species. IVM bypasses traditional superovulation methods, where several hormonal injections are administered. Unfortunately, the pregnancy rates using IVM oocytes is less than those matured <em>in vivo</em>. This has impeded the uptake of the technology in ART laboratories. This review aims to explore the effects of multiple elements during IVM, discussing not only those which improve and impact on oocyte viability but those that can have deleterious effects on the oocyte.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"2 2","pages":"Pages 31-36"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071222000114/pdfft?md5=26edf87e908db3a8e742e3fb9afe6d8b&pid=1-s2.0-S2667071222000114-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41700181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}