Reproduction and breeding最新文献

{"title":"Genetic variability, heritability and genetic advance in Iranian local rice genotypes for yield, and some agronomic traits","authors":"Mostafa Modarresi","doi":"10.1016/j.repbre.2025.12.003","DOIUrl":"10.1016/j.repbre.2025.12.003","url":null,"abstract":"<div><div>Rice (<em>Oryza sativa</em> L.) is a cornerstone of global food security, providing 20 % of the world's dietary energy and serving as a staple for half the global population. In Iran, rice supports food supply and economic stability, yet faces challenges like disease susceptibility and low yields. This study evaluated the genetic diversity and heritability of agronomic traits in 26 Iranian rice genotypes to inform breeding strategies for enhanced yield and adaptability. Conducted during the 2021–2023 years at the Rice Research Institute of Iran in Guilan Province, the experiment employed a Randomized Complete Block Design with three replications. Traits assessed included grain yield, plant height, panicle number, thousand-grain weight, grain dimensions, awn length, and days to maturity. Analysis of variance revealed significant genetic variation (p &lt; 0.01) across all traits. Grain yield ranged from 3.00 to 4.00 t ha⁻¹, with high-yielding genotypes like Ali Kazemi and Hashemi identified. Broad-sense heritability was high (0.886–0.974), indicating strong genetic control, particularly for grain shape (H² = 0.974) and awn length (H² = 0.997). Genotypic and phenotypic coefficients of variation were closely aligned, suggesting minimal environmental influence, with genetic advance as a percentage of the mean highest for awn length (206.93 %) and grain shape (32.71 %). Correlation analysis showed positive associations between grain yield and grain width (r = 0.29), while path analysis highlighted thousand-grain weight (0.226, p = 0.015) and productive tillers (0.190, p = 0.041) as key yield contributors. Hierarchical clustering grouped genotypes into four distinct clusters, reflecting diverse genetic profiles. These findings underscore the potential for targeted selection and hybridization to develop high-yielding, adaptable rice cultivars, enhancing Iran's rice production and global food security.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 9-20"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A conserved role of the N6-methyladenosine (m6A) modification in spermatogenesis","authors":"Ayla Sinclair ,&nbsp;Antonio Rockwell","doi":"10.1016/j.repbre.2025.12.005","DOIUrl":"10.1016/j.repbre.2025.12.005","url":null,"abstract":"<div><div>Epitranscriptomics is an emerging field that addresses questions about the way RNA modifications affect gene expression. There are numerous known RNA modifications with N⁶-methyladenosine (m⁶A) being the most prevalent. A wide range of biological processes such as spermatogenesis rely on the m⁶A modification. From an evolutionary perspective, m⁶A and its regulatory components are conserved during the process of spermatogenesis, owing to the efficiency and significance of the process. Studies in a wide range of organisms have helped to clarify the role of m⁶A in sperm development. Here we summarize articles focused on the role of m⁶A during spermatogenesis in various animal models. We incorporate animal studies that have been conducted on one of the three major m⁶A regulatory components, writers, readers and erasers. Additionally, we have added information from studies on m⁶A transcriptome wide investigations. Incorporating these investigations provides perspective to the conserved nature of the process, while contextualizing the impact research on spermatogenesis may have broadly.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 36-42"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cryopreservation on sperm telomere length in infertile men: A comprehensive review","authors":"Swathy Yuvaraj ,&nbsp;Radha Vembu ,&nbsp;Teena Koshy ,&nbsp;Yamini Asokan","doi":"10.1016/j.repbre.2026.01.003","DOIUrl":"10.1016/j.repbre.2026.01.003","url":null,"abstract":"<div><div>Cryopreservation a routine procedure in reproductive medicine, widely used for fertility preservation and donation. Among many implications the effect of cryopreservation on sperm telomere length (STL) is being researched, in recent times STL is regarded as a probable biomarker for sperm quality, chromosomal integrity and reproductive ability. This review aims to understand and highlight the current literature and future scopes of changes in STL following cryopreservation in infertile men.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 70-76"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in breeding technologies for enhancing mustard yield and stress resilience: A comprehensive review","authors":"Supratim Sadhu ,&nbsp;Paresh Chandra Kole ,&nbsp;Sohum Roy ,&nbsp;Sagnik Ghosh ,&nbsp;Mehdi Rahimi","doi":"10.1016/j.repbre.2026.01.001","DOIUrl":"10.1016/j.repbre.2026.01.001","url":null,"abstract":"<div><div>Mustard (<em>Brassica juncea</em>) is a major oilseed crop, yet its yield and stability are constrained by a narrow genetic base and increasing biotic and abiotic stresses under changing climates. Although substantial progress has been made in mustard improvement, recent literature often treats traditional breeding, genomics, genome editing, hybrid systems and stress physiology as separate domains, limiting translation into integrated breeding pipelines. This review synthesizes recent advances in plant breeding technologies has significantly transformed strategies for increasing mustard yield by enabling precise modification of key agronomic characteristics and quality related traits. The quality of mustard oil has improved because of the precise manipulation of glucosinolate levels in mustard plants made possible by CRISPR/Cas9 technology, as demonstrated in experimental studies on <em>Brassica juncea</em>. Hybrid seed production has been significantly enhanced through the Barnase-barstar gene system, which enables stable male sterility and efficient exploitation of heterosis, leading to improved yield stability and robustness. In parallel, microsatellite markers have become indispensable tools for genetic diversity analysis, trait mapping and marker-assisted breeding. In India, the development of hybrids such as DMH-11 (Dhara Mustard Hybrid - 11) signifies the integration of molecular breeding and genetic engineering approaches aimed at enhancing productivity and reducing dependence on edible oil imports. Collectively, these advances highlight the critical role of genetic manipulation and molecular breeding strategies in improving stress resilience and achieving sustainable gains in mustard productivity.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 43-52"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiological and molecular insights into grass carp adaptation to eutrophic environments","authors":"Fangyuan Peng ,&nbsp;Xueru Qian ,&nbsp;Yizhen Wang ,&nbsp;Shuxia Yao ,&nbsp;Lingwei Tang ,&nbsp;Chigang Huang ,&nbsp;Deng Pan ,&nbsp;Jinhui Liu ,&nbsp;Wen Fu ,&nbsp;Liangyue Peng ,&nbsp;Wenbin Liu","doi":"10.1016/j.repbre.2026.01.002","DOIUrl":"10.1016/j.repbre.2026.01.002","url":null,"abstract":"<div><div>In recent years, freshwater fisheries have experienced rapid development worldwide. However, poor water management practices associated with intensive, high-density aquaculture systems may accelerate eutrophication within aquaculture systems—posing a major challenge to the sustainable development of the finfish aquaculture industry. Grass carp (<em>Ctenopharyngodon idella</em>), one of the most widely cultivated herbivorous fish species characterized by rapid growth and high environmental adaptability, plays a pivotal role in the field of national aquaculture. To investigate the adaptive mechanisms of grass carp in eutrophic environments, a 30-day controlled culture experiment was conducted using lightly eutrophic water as the baseline rearing environment [Eutrophication levels were defined as follows: Lightly eutrophic: total nitrogen (TN) = 1.00 mg/L, total phosphorus (TP) = 0.100 mg/L; Moderate eutrophic: TN = 2.00–6.00 mg/L, TP = 0.200–0.600 mg/L; Severe eutrophic: TN = 9.00–16.00 mg/L, TP = 0.900–1.300 mg/L]. Two grass carp strains—the common strain and the gynogenetic disease-resistant strain—were reared under controlled eutrophic conditions.</div><div>Using a multi-faceted analytical approach that included transmission electron microscopy, histological paraffin sectioning, blood smear analysis, quantitative real-time reverse transcription PCR (qRT-PCR), and antioxidant enzyme activity assays, this study revealed that eutrophic conditions induce structural damage to multiple tissues and organs in grass carp. Furthermore, beneficial responses of the antioxidant system to environmental stress and immune activation were observed. Notably, the gynogenetic disease-resistant strain exhibited superior adaptability and more efficient inflammatory regulation than the common strain. Integrated transcriptomic analysis revealed that grass carp mitigate the adverse effects of eutrophication by upregulating metabolic processes and activating immune signaling pathways. Specifically, the peroxisome proliferator-activated receptor (PPAR) and vascular endothelial growth factor (VEGF) signaling pathways were found to function synergistically, thereby promoting the adaptation of grass carp to eutrophic environments.</div><div>This study establishes a molecular basis for comprehensively elucidating the environmental adaptation mechanisms of fish, providing significant insights for the selective breeding of stress-tolerant aquatic species and the advancement of sustainable freshwater aquaculture practices.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 53-69"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145978020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of intermuscular bones in hybrid of female (megalobrama amblycephala × culter alburnus) × male megalobrama amblycephala and its parents","authors":"Kaikun Luo ,&nbsp;Zezhou Zhong ,&nbsp;Chengxi Liu,&nbsp;Zhonghua Peng,&nbsp;Binchao Liang,&nbsp;Haipeng Guo,&nbsp;Jing Wang,&nbsp;Wuhui Li,&nbsp;Chang Wu,&nbsp;Fangzhou Hu,&nbsp;Shi Wang,&nbsp;Yude Wang,&nbsp;Shengnan Li,&nbsp;Liang Guo,&nbsp;Zhongyuan Shen","doi":"10.1016/j.repbre.2025.12.001","DOIUrl":"10.1016/j.repbre.2025.12.001","url":null,"abstract":"<div><div>Based on the formation of the hybrid lineage of blunt-snout bream (megalobrama amblycephala, BSB) × topmouth culter (culter alburnus, TC), a new kind of hybrid fish is produced by hybridizing the female diploid F1 hybrid of BSB × TC (short for BT) with male BSB. In order to investigate intermuscular bones variety in the distant hybrid progeny, the number, morphology, and distribution of intermuscular bones in the hybrids and its parents are analyzed. The number of intermuscular bones in BSB, TC, BT and the hybrid (BT × BSB, short for BTB) ranges from 112 to 119 (x =116.1), 126 to 136 (x =130.2), 121 to 139 (x =129.6) and 116 to 125 (x =119.9), respectively. The number in BTB is significantly lower than TC and BT (P &lt; 0.05). The average number of intermuscular bones in each sarcomere is 0.6971 (BTB), 0.7256 (TC), 0.7570 (TC) and 0.8100(BT), respectively, which indicates that the BTB has the lowest intermuscular bones in each sarcomere. In morphology, the intermuscular bone of these four kinds of fish are divided into seven shape categories and higher in the anterior intermuscular bones than in the posterior body. These studies show that the BTB not only inherits the advantage traits from BSB (with less intermuscular bones), but also gains variant traits (with lowest intermuscular bones in each sarcomere), which are beneficial to its direct edible and deep processing in industry. This study proves that it is possible to produce superior varieties with lower intermuscular bones by distant hybridization, which has some significance in fish genetic breeding.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145760836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic variation, heritability, and genetic advance among elite sorghum [Sorghum bicolor (L) Moench] genotypes for yield and yield associated traits under moisture stress areas","authors":"Werkissa Yali ,&nbsp;Gudeta Nepir","doi":"10.1016/j.repbre.2025.12.002","DOIUrl":"10.1016/j.repbre.2025.12.002","url":null,"abstract":"<div><div>In dry lowland regions of the world, sorghum (<em>Sorghum bicolor</em> (L) Moench) is a significant crop extensively farmed for food, feed, fodder, and fuel. Despite extensive breeding work, sorghum production and productivity remain low in Ethiopia. To create superior genotypes in breeding programs, it is necessary to comprehend the existence and extent of genetic diversity. Therefore, this research aimed to evaluate the importance of genetic diversity, heritability, and genetic advance in sorghum genotypes. Using an alpha lattice design with two replications, a total of 72 genotypes were assessed during the main cropping season of 2020 in Miesso in Eastern Ethiopia and Kobo in Northern Ethiopia. For every trait, a combined analysis of variance revealed a significant difference (p &lt; 0.01) between the genotypes. Grain yield, the number of heads per plot, and the number of stands at harvest had the highest genotypic and phenotypic coefficients of variation, while the number of days to flowering, days to maturity, grain filling period, leaf length, and leaf width had the lowest. Genetic advance as a percentage of the mean (GAM) varied from 2.28 % for the number of days to maturity to 97.04 % for grain yield, while broad sense heritability ranged from 26.46 % for panicle width to 89.67 % for grain yield. The genotypes ETSC14804-4-2 (4.97 t/ha), ETSC14695-1-2 (4.7 t/ha), and ETSC14715-3-1 (4.46 t/ha) were found to be high-yielders in comparison to the others based on the current data. Still, more research is required to make better recommendations.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 21-26"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovation of Siluriformes fish germplasm and its research progress","authors":"Sijin Fan ,&nbsp;Yu Hu ,&nbsp;Xinyue Deng ,&nbsp;Yalan Zhang ,&nbsp;Wei Chen ,&nbsp;Gaode Zhong ,&nbsp;Xin Wang ,&nbsp;Zebin Huang ,&nbsp;Hanyu Liu ,&nbsp;Fangzhou Hu ,&nbsp;Chang Wu ,&nbsp;Wuhui Li ,&nbsp;Shi Wang ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2026.02.001","DOIUrl":"10.1016/j.repbre.2026.02.001","url":null,"abstract":"<div><div>Siluriformes fish are widely distributed across China and are highly favored by consumers for their strong adaptability, rapid growth, palatable flesh, and low number of intermuscular bones. These attributes render them particularly well-suited for artificial aquaculture, highlighting their considerable economic value. Globally, the order comprises approximately 34 families and over 2300 species, representing 6.61% of all known fish species worldwide. In terms of genetic improvement, breeding programs have primarily focused on enhancing growth performance, leading to the successful development of several high-quality new varieties. For example, selective breeding has successfully produced the longsnout catfish (<em>Leiocassis longirostris</em>) “Chuanjiang No. 1”, which effectively mitigates issues related to germplasm degradation. In the field of hybrid breeding, superior varieties including channel catfish (<em>Ictalurus punctatus</em>) “Jiangfeng No. 1”, hybrid yellow catfish (<em>Pelteobagrus fulvidraco</em>) “Huangyou No. 1” and “Baixiong No. 1” have been developed, demonstrating significant advantages in growth rate, yield, and stress resistance. Regarding sex-controlled breeding, YY super-male yellow catfish and other lineages have been established through hormonal sex reversal, gynogenesis, and test cross validation. Significant advances have also been achieved in modern molecular breeding technologies. Molecular marker-assisted breeding has enabled the identification of X/Y chromosome-linked markers—such as AFLP, SCAR, SSR, and SNP—for precise trait locus selection. Furthermore, cutting-edge techniques including genome-wide selection, gene editing, stem cell-mediated nuclear transfer, and transgenesis have been successfully implemented. Collectively, these approaches have contributed to the establishment of a diversified and integrated breeding technology system. This paper provides a comprehensive review of the research history, industrial development, germplasm resources, and recent progress in the application of aquatic genetic breeding technologies for germplasm innovation in Siluriformes fish in China.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 77-90"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of haploid zebrafish and analysis of resultant developmental defects and aberrant gene expression","authors":"Xing Guo ,&nbsp;Ruoyu Lin ,&nbsp;Simin Huang ,&nbsp;Jing Huang ,&nbsp;Minggui Jiang ,&nbsp;Xiudan Yuan ,&nbsp;Jinhui Liu ,&nbsp;Wen Fu ,&nbsp;Wenbing Liu ,&nbsp;Liangyue Peng","doi":"10.1016/j.repbre.2025.12.004","DOIUrl":"10.1016/j.repbre.2025.12.004","url":null,"abstract":"<div><div>Haploid syndrome represents a fatal phenotypic condition that manifests during the embryonic stages of teleost fish, posing challenges for genetic screening and aquaculture breeding. This research employs zebrafish as a model to pinpoint the critical spectrum of developmental abnormalities that arise post-haploid induction and to uncover the primary transcriptomic dysregulation network along with potential functional genes. Haploid zebrafish embryos were produced using gynogenesis facilitated by sperm that had been exposed to ultraviolet radiation. The haploid condition was verified through flow cytometry and chromosome karyotype assessments. Whole-transcriptome sequencing was conducted via RNA sequencing (RNA-seq) across various phenotypic categories to pinpoint differentially expressed genes. Enrichment analysis, co-expression network construction, and quantitative reverse transcription PCR (qRT-PCR) were utilized to identify relevant functional modules and key regulatory genes. The haploid embryos displayed characteristic phenotypes such as body axis curvature, pericardial edema, and early mortality, with a hatching rate significantly lower than that of the diploid control group. Transcriptomic analysis identified 2247 differentially expressed genes, with 13 genes consistently recognized across several group comparisons. Their functional enrichment was notably focused on the FGF/Wnt signaling pathway, mitochondrial translation, ion transport, cell cycle regulation, and other essential hub genes linked to developmental anomalies. Additionally, their expression levels showed a significant dose-dependent correlation with the extent of malformations. The results of this study suggest that the lethal developmental issues associated with haploid syndrome stem from the combined dysregulation of multiple pathways, establishing a set of key target genes for potential intervention. This research lays a theoretical groundwork and offers practical strategies for improving haploid survival rates, holding considerable promise for advancements in fish haploid breeding technology.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"6 1","pages":"Pages 27-35"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of pollen viability and germination in banana (Musa acuminata) diploids under humid tropical conditions","authors":"Stivens Flores-Guillén ,&nbsp;Jorge Sandoval-Fernández ,&nbsp;Edson Perito Amorim ,&nbsp;Rafael Segura-Mena ,&nbsp;Fabián Echeverría-Beirute","doi":"10.1016/j.repbre.2025.11.001","DOIUrl":"10.1016/j.repbre.2025.11.001","url":null,"abstract":"<div><div>Genetic improvement of banana (<em>Musa acuminata</em>) is essential to overcome the challenges that limit its sustainable production, including critical diseases such as <em>Fusarium</em> tropical race 4 (Foc TR4) and Black Sigatoka caused by <em>Pseudocercospora fijiensis</em>. This study aimed to evaluate the viability and germination of pollen from three improved diploids <em>Musa acuminata</em> (accessions M53, 013018-01, and 013004-04), along with a globally recognized wild accession 'Calcutta 4' and a non-fertile commercial cultivar <em>M. acuminata cv.</em> Pisang Mas as a contrasting treatment, under humid tropical conditions in Turrialba, Costa Rica. Pollen grains were collected at different times (7:30, 10:30, and 13:30) and from different positions of the male inflorescence (proximal, medial, and distal). Results showed significant differences in pollen germination based on genotype, sampling time, and position. The diploid M53 had the highest germination percentage (21.2 %), with distal positions and afternoon sampling being the most favorable, followed by diploids 013018-01, Calcutta 4, and 013004-04 with germination rates of 16 %, 8 %, and 5 %, respectively. All genotypes exhibited viability percentages above 90 %. The cultivar Pisang Mas showed no germination in any of the evaluated treatments due to its sterility. These findings provide important insights for genetic improvement programs, facilitating the selection of progenitors with better reproductive capacities under tropical conditions and strengthening the genetic base for research with Cavendish subgroup representatives to address current biotic and abiotic threats.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 222-230"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}