Reproduction and breeding最新文献

{"title":"Quantitative proteomic analysis revealed the protein expression characteristics of primary follicle subtypes in zebrafish","authors":"Guangjing Zhang ,&nbsp;Jing Huang ,&nbsp;Liangyue Peng ,&nbsp;Jinhui Liu ,&nbsp;Wenbin Liu ,&nbsp;Wen Fu","doi":"10.1016/j.repbre.2025.03.001","DOIUrl":"10.1016/j.repbre.2025.03.001","url":null,"abstract":"<div><div>Follicles are the basic structure and functional unit of ovaries. In the previous study, we classified primary follicles (PFs) of zebrafish into four subtypes, compared the DNA methylation and RNA expression characteristics among different subtypes follicles, and preliminarily analyzed the molecular mechanism of PFs development. However, the protein expression characteristics of the four subtypes of PFs remain poorly understood. In this study, a total of 9635 proteins were identified in the four subtypes PFs of zebrafish by proteomic analysis. The proteins highly expressed in the PF-ii and PF-iii were enriched in Notch, MAPK, and mTOR signaling pathways. By combined transcriptomic and proteomic analysis screened five key candidate molecules involved in PFs developmental regulation. These results provide reference to the molecular mechanisms underlying follicular development in fish.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 21-26"},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression of liver–gonadal axis genes reveals the long–term effects of exogenous estradiol on gonad development and liver function in GIFT tilapia","authors":"Ye Yuan ,&nbsp;Zexun Zhou ,&nbsp;Shandong Chen ,&nbsp;Yunyun Liu ,&nbsp;Yongchun Li ,&nbsp;Yan Miao ,&nbsp;Shi Wang ,&nbsp;Zhongyuan Shen ,&nbsp;Lei Zeng ,&nbsp;Jun Xiao ,&nbsp;Qinbo Qin ,&nbsp;Wuhui Li","doi":"10.1016/j.repbre.2025.02.003","DOIUrl":"10.1016/j.repbre.2025.02.003","url":null,"abstract":"<div><div>The aim of this study was to investigate the effects of exogenous estradiol on the expression of the liver<strong>–</strong>gonadal axis gene in GIFT tilapia. Larval fish (5 dph) were fed a diet with 100 mg kg⁻¹ 17α–ethynylestradiol (EE2) for 30 days and then transferred to a conventional diet. Sex reversal statistics revealed that more than 60 % of the genotypic male fish transformed into pseudofemales. Histological results showed that the oocytes in pseudofemales developed asynchronously and had a prolonged maturation time compared to those in normal female fish. Global expression and DNA methylation analyses revealed that the expression patterns of the gonad and liver genes in pseudofemales were similar to those in XX female fish. Compared to XY male fish, the differentially expressed genes (DEGs) in pseudofemale gonads were enriched in FoxO signaling pathway, oocyte meiosis and other hormone–regulated pathways, which may affect the growth and development of the ovary. Moreover, the DEGs in liver were enriched mainly in protein digestion and absorption, steroid biosynthesis, and some disease and energy metabolism pathways, which may affect liver immunity and energy metabolism. Furthermore, the co-upregulated genes in the liver and gonads were enriched mainly in steroid biosynthesis and glycolysis/gluconeogenesis, whereas the co-downregulated genes were enriched in GnRH secretion and oxytocin signaling pathway. In addition, some genes associated with hormone secretion, growth, metabolism, and immunity in the liver‒gonad axis, such as <em>Oxt</em>, <em>Spoll</em> and <em>Piwi12,</em> were activated, whereas <em>Hox</em>, <em>cyp21a</em>, <em>gas1</em> and other genes were inhibited. These results indicated that EE2 affects gene expressions in liver and gonad tissues through the hypothalamic–pituitary–gonadal–liver (HPGL) axis.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 11-20"},"PeriodicalIF":0.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring spatio-temporal heterogeneity and inter-domain ecological networks of biological community in a marine ranching habitat: Implications for fishery resources conservation","authors":"Zeng Lei ,&nbsp;Chen Guobao ,&nbsp;Zheng Tingting ,&nbsp;Chen Yuxiang ,&nbsp;Yu jie ,&nbsp;Li Wuhui","doi":"10.1016/j.repbre.2025.02.001","DOIUrl":"10.1016/j.repbre.2025.02.001","url":null,"abstract":"<div><div>Habitat changes in marine ranching can cause variations in biological resources and community structure. However, the complex inter-domain ecological network (IDEN) in this particular habitat are not well understood. Thus, we employed field surveys and multivariate statistical analyses to explore the spatio-temporal heterogeneity and the IDEN of biological communities within a marine ranching in southern Yintan of Beihai City, China. Our findings indicated that seasonal succession and artificial reefs (ARs) deployment had simultaneous effects on both biotic and abiotic factors, and the former effect was more pronounced. Based on orthogonal partial least squares discriminant analysis (OPLS-DA), various biota (phytoplankton, zooplankton, and nekton) can be broadly categorized into four groups on seasonal and spatial scales. Variable importance projection (VIP) and redundancy analysis (RDA) demonstrated that autumn biomarkers such as <em>Hemidiscus hardmannianus</em>, <em>Oikopleura rufescens</em>, <em>Lagocephalus lunaris</em> were positively correlated with water temperature (WT), salinity (Sal) and inorganic nitrogen (IN), whereas spring biomarkers such as <em>Rhizosolenia robusta</em>, <em>Euconchoecia aculeata</em>, <em>Penaeus penicillatus</em> were positively correlated with dissolved (DO), chemical oxygen demand (COD), and inorganic phosphorus (IP). The IDEN analysis revealed that 23 species such as <em>Chaetoceros densus</em>, <em>Centropages tenuiremis</em>, and <em>Parargyrops edita</em> played a key role in maintaining the stability of the biological communities in the studied sea area. This study can provide novel insights and theoretical supports for the ecological effects analysis and species protection in marine ranching.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of carnitine and N-Acetyl-Cysteine supplementation on sperm parameters and pregnancy outcomes in idiopathic male infertility: A systematic review and meta-analysis of randomized control trials","authors":"Yazan Ranneh ,&nbsp;Mohammed Hamsho ,&nbsp;Abdulmannan Fadel ,&nbsp;Hiba Mahgoub Ali Osman ,&nbsp;Elshazali Widaa Ali ,&nbsp;Nahla Hwaitalla Mohammed Kambal","doi":"10.1016/j.repbre.2025.02.002","DOIUrl":"10.1016/j.repbre.2025.02.002","url":null,"abstract":"<div><div>This systematic review and meta-analysis aimed to evaluate the impact of L-carnitine (LC), L-acetyl-carnitine (LAC), and N-acetyl-cysteine (NAC) on sperm parameters and pregnancy outcomes in men with idiopathic infertility. A comprehensive search was conducted in PubMed, Embase, Scopus, and Google Scholar databases up to January 31, 2024. Of the 2293 initial citations, 109 studies were eligible, and 14 RCTs involving 1453 men were included in the analysis. LC supplementation significantly increased the sperm concentration (MD = 6.85), total motility (MD = 10.41 %), and normal morphology (MD = 1.78 %). LAC supplementation improved total motility (MD = 17.03 %) and forward motility (MD = 13.5 %), whereas LC + LAC supplementation showed no significant effects. Carnitine combinations (CCs) increased sperm count (MD = 16.75) and volume (MD = 0.19 mL), with improved forward motility (MD = 7.70 %). NAC supplementation enhanced sperm concentration (MD = 3.09) and normal morphology (MD = 1.43 %) and volume (MD = 0.61 mL). LC and CC significantly reduced follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels. Meta-analysis demonstrated significant heterogeneity in many comparisons. LC, CCs, and NAC may improve sperm parameters in idiopathic infertile men, including sperm concentration, motility, morphology, and volume as well as pregnancy outcomes. However, the overall quality of the included studies was low and high heterogeneity was observed. Future well-designed RCTs with longer durations and fixed doses are warranted to confirm these findings and to assess their impact on pregnancy outcomes.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 74-83"},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of photoperiod and temperature on melatonin, growth hormone, estradiol, and vitellogenin levels in female African catfish (Clarias gariepinus) reproduction cycle","authors":"Epro Barades ,&nbsp;Iskandar ,&nbsp;Ibnu Dwi Buwono ,&nbsp;Yuli Andriani","doi":"10.1016/j.repbre.2025.01.002","DOIUrl":"10.1016/j.repbre.2025.01.002","url":null,"abstract":"<div><div>This study aimed to investigate the hormone levels of melatonin (Mel), estradiol (E2), vitellogenin (VTG), and growth hormone (GH) in inducing reproduction cycles in female African catfish, <em>Clarias gariepinus</em>, to develop actionable strategies that directly contribute to the efficiency and sustainability of African catfish farming. The treatments involved in this study are three photoperiod variations (L8:D16, L4:D20, and L0:D24) combined with two different temperatures (28 °C and 32 °C) during 90 days of culture (doc). Serum hormone levels were measured using ELISA, and egg diameter was measured using a microscope every 30 days. The results showed that the biological rhythm of the reproductive cycle of African catfish was accelerated by constant exposure to a photoperiod of L0:D24 and 28 °C for 30 days. In this condition, the levels of hormones involved in the reproductive such as Mel (89.82 ± 5.49 ng/mL), E2 (1.66 ± 0.02 ng/mL), VTG (100.96 ± 0.27 ng/mL) and GH (0.33 ± 0.02 ng/mL) with an average egg diameter of 1.15 ± 0.07 mm. These results highlight the complex interplay between photoperiod, temperature, and reproductive physiology in African catfish, suggesting that environmental manipulation could be a valuable tool for optimizing breeding conditions in aquaculture. The conclusion of this study is that manipulating the photoperiod and temperature is an effective and economical approach to stimulate fish spawning. These findings have important implications for African catfish breeding practices, as they provide a clear and actionable strategy for improving reproductive performance.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 54-66"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling reveals the mechanism of fast growth of an allodiploid hybrid fish","authors":"Ziyi Huang ,&nbsp;Siyang Huang ,&nbsp;Hongwen Liu ,&nbsp;Bei Li ,&nbsp;Jianming Yu ,&nbsp;Xuanyi Zhang ,&nbsp;Fanglei Liu ,&nbsp;Lujiao Duan ,&nbsp;Qingfeng Liu","doi":"10.1016/j.repbre.2025.01.001","DOIUrl":"10.1016/j.repbre.2025.01.001","url":null,"abstract":"<div><div>Distant hybridization is an important technique and widely used in fish genetic breeding. In previous research, we obtained the allodiploid hybrid (BR, 2n = 49) derived from the inter-subfamily hybridization of female blunt snout bream (<em>Megalobrama amblycephala</em>, BSB, 2n = 48) and male rare gudgeon (<em>Gobiocypris rarus</em>, RG, 2n = 50), which had obvious growth advantages over the male parent RG. However, less is known about the potential mechanisms of fast growth in BR. In this study, the liver transcriptomes of BR and parents were analyzed by RNA-seq technology. A total of 4.73 × 10⁸ clean reads and 11,040 co-expressed unigenes were obtained. The results showed that the differentially expressed genes in BR were asymmetric compared with those in both parents. Interestingly, the genome-wide expression level dominance (ELD) was biased toward the BSB genome in BR, even if BR inherited one more chromosome from RG than that from BSB. The KEGG analysis of ELD genes showed that most metabolism pathways were enriched in ELD-BSB, such as biosynthesis of unsaturated fatty acids, fatty acid elongation, pyruvate metabolism, and so on. Furthermore, the growth genes of BR were most enriched in the ELD-BSB (34.38 %) than in other categories. The expression levels of some key hormone genes (<em>ghra</em>, <em>ghrb</em>, <em>igf1</em>, <em>igfals</em>, <em>igf2bp2</em>, and <em>grb2</em>) were upregulated in BR compared with those in RG. This study provided an insight into the potential molecular mechanism of the fast growth in BR, which will be of great significance in fish genetic breeding.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 67-73"},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, expression, and localization of Tekt1 in sterile allotriploid crucian carp","authors":"Shuxin Zhang ,&nbsp;Liran Zhang ,&nbsp;Faxian Yu,&nbsp;Xinge Ouyang,&nbsp;Haoxiang Yang,&nbsp;Qining Zuo,&nbsp;Yujie Huang,&nbsp;Xin Chen,&nbsp;Shengnan Li,&nbsp;Min Tao","doi":"10.1016/j.repbre.2024.12.002","DOIUrl":"10.1016/j.repbre.2024.12.002","url":null,"abstract":"<div><div>Tektins (TEKTs) are constitutive proteins of microtubules associated with flagella, cilia, basal bodies, and centrioles. As one of the testis-specific candidate markers, <em>Tekt</em>1, the first identified member of the TEKT family in mammals, is intimately linked to the formation of sperm flagella and may play a pivotal role in flagellar stability and sperm motility. However, studies on <em>Tekt</em>1 in fish species are still relatively understudied. In this study, the full-length cDNAs of <em>Tekt</em>1 were respectively 1727 bp and 1696 bp in allotriploid crucian carp and diploid red crucian carp, which both comprised a 1209 bp open reading frame (ORF) encoding 402 amino acids. Conversely, the diploid common carp possessed a cDNA length of 1771 bp, characterized by a 1218 bp ORF encoding 405 amino acids. The Western blot analysis revealed that the expression level of Tekt1 protein in the testes of sterile allotriploid crucian carp was markedly decreased in comparison to that of fertile diploid red crucian carp during both pre-spermiation and spermiation periods. The immunohistochemistry analysis revealed abnormalities in the spermiogenesis of allotriploid crucian carp, showcasing a distinctive localization pattern of Tekt1 exclusively present in spermatids, in contrast to diploid red crucian carp, where Tekt1 was detected in both spermatids and spermatozoa. Taken together, these findings suggested differential expression of Tekt1 during spermiogenesis between allotriploid and diploid species, and indicated that the decreased expression of Tekt1 protein in allotriploid crucian carp might be associated with male sterility. Furthermore, these results pave the way for further exploration of reproductive characteristics in male allotriploid crucian carp and offer a theoretical foundation for future research on polyploid breeding.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 45-53"},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly efficient disruption of tyrb gene using CRISPR/Cas9 in grass carp (Ctenopharyngodon idella)","authors":"Pengfei Zhao ,&nbsp;Jiaxiang Cheng ,&nbsp;Liang Zhang ,&nbsp;Wenbo Li,&nbsp;Shengfei Dai,&nbsp;Minghui Li,&nbsp;Deshou Wang,&nbsp;Xingyong Liu","doi":"10.1016/j.repbre.2024.12.003","DOIUrl":"10.1016/j.repbre.2024.12.003","url":null,"abstract":"<div><div>Grass carp (<em>Ctenopharyngodon idella</em>) is the most important economic freshwater fish species in China. The stable production of high-quality grass carp depends significantly on excellent germplasm. In recent years, the generation of new germplasm based on genome editing has been applied to various cultured fish species. However, until now, there has been very few reports on the application of genome editing technology in grass carp. In this study, one-cell-stage embryos of grass carp were acquired through hormone-induced artificial spawning, thereby enabling the performance of genome editing in this species. The <em>tyrb</em> gene was isolated and chosen as the target of CRISPR/Cas9, because of its easily observable phenotype in F0 mutants. RT-PCR results indicated a high expression level of the <em>tyrb</em> gene in both skin and fin tissues. Subsequently, after the microinjection of the guide RNA (gRNA) and Cas9 protein mixture, targeted mutations were successfully identified through Sanger sequencing. Phenotypic analysis of the F0 mutants revealed that the disruption of <em>tyrb</em> led to a distinct golden phenotype, accompanied by a reduction or even absence of melanophores. Moreover, our data demonstrated that the combined utilization of two or three gRNAs caused large DNA fragment loss and a higher mutation rate in the F0 generation. Overall, this represents an application of CRISPR/Cas9 genome-editing technology in grass carp and may hold great significance for the future generation of new golden grass carp germplasm.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 38-44"},"PeriodicalIF":0.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of single-wall carbon nanotube and its biocompatibility to human hepatocytes","authors":"Hatef Rahim Sabbaghizadeh ,&nbsp;Arshin Oskoueian ,&nbsp;Amir Hossein Ashtari","doi":"10.1016/j.repbre.2024.12.001","DOIUrl":"10.1016/j.repbre.2024.12.001","url":null,"abstract":"<div><div>Single-wall carbon nanotubes (SWCNTs) have emerged as promising nanocarriers for targeted cancer drug delivery due to their unique structural properties. However, their cytotoxicity remains a significant challenge, as the biocompatibility of SWCNTs with human cells, particularly hepatocytes, is crucial for their clinical application. The toxicity of SWCNTs is influenced by factors such as nanoparticle size, morphology, surface chemistry, and the presence of impurities. In this study, we aimed to synthesize highly pure SWCNTs and assess their biocompatibility with human hepatocyte cells.</div><div>SWCNTs were fabricated using a modified chemical vapor deposition (CVD) method, followed by a two-step acid purification technique. Raman spectroscopy and electron microscopy confirmed a high purity level of 99.8 %. The biocompatibility of the purified SWCNTs was evaluated using an in vitro model with human hepatocytes. Results indicated that high concentrations of SWCNTs (&gt;50 μg/ml) significantly reduced cell viability, increased lactate dehydrogenase leakage, and elevated lipid peroxidation, while simultaneously suppressing antioxidant enzyme activity.</div><div>Flow cytometry analysis further revealed that exposure to high concentrations of SWCNTs induced apoptosis in hepatocytes. Molecular analysis of key biomarkers demonstrated upregulation of TNF-α, IL1β, NF-kB, and iNOS, alongside downregulation of nrf2 gene and protein expression. These alterations contribute to the mechanisms underlying SWCNT-induced oxidative stress and apoptosis in human hepatocyte cells. Despite the high purity of SWCNTs, their cytotoxic effects may be attributed to their inherent physical properties, including rigidity, surface area, and fiber length.</div><div>In conclusion, while SWCNTs hold great potential for cancer drug delivery, managing their toxicity remains critical for their future therapeutic applications.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 29-37"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143175459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Widening the tool set for breeding superior banana cultivars. Can new techniques of pollen handling and pollination help overcome the lack of recombinant seed in banana breeding?","authors":"Ralf Bodo Trognitz","doi":"10.1016/j.repbre.2024.11.001","DOIUrl":"10.1016/j.repbre.2024.11.001","url":null,"abstract":"<div><div>Pollen tube growth (PTG) monitoring was applied to florets of domesticated bananas and plantains. Observations were made in UV microscopy after Aniline Blue staining specific for callose, a carbohydrate of pollen tubes. It was found that total pollen grain numbers on a stigma and total pollen tubes developing after sufficient time for growing to reach the ovules within a female floret ranged from nil to few. This suggested the standard hand pollination technique, HP, may be insufficient to achieve desired pollination quality and respective fertilization for seed development. Pollen deposition and pollen tube growth was at least ten times larger when applying the newly developed pollen-anther-stigma, PAS, technique of pollination. This PAS technique includes mechanically working wilted anther pieces holding and exposing the pollen into the female stigma. PAS provided the potential for maximum seed formation depending on genetic limitations that may be present. PAS is therefore recommended for use in investigations of reproductive processes, such as sterility and self-incompatibility, and it can help increasing the production of recombinant seeds in planned crosses for breeding and selection.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 1","pages":"Pages 23-28"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}