Reproduction and breeding最新文献

{"title":"Optimum gamma radiation doses to enhance genetic diversity in selected cowpea (Vigna unguiculata) genotypes","authors":"Pamwenafye I. Nanhapo ,&nbsp;Johanna S. Valombola ,&nbsp;Maliata A. Wanga ,&nbsp;Konis Elungi ,&nbsp;Simon K. Awala ,&nbsp;Lydia N. Horn","doi":"10.1016/j.repbre.2024.01.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.01.001","url":null,"abstract":"<div><p>Cowpea (<em>Vigna unguiculata</em> L.) improvement is necessary to increase its contribution to food and nutrition security in the impoverished regions such as the Sub-Saharan African Region. Genetic variability is fundamental to selecting parental genetic resources with agronomic and economic important traits for recombination. Gamma radiation is one of the valuable mutagens for creating novel mutant populations with allelic combinations needed to develop varieties with the desired traits. In this study, we determined the optimum dosage of gamma irradiation to use in breeding programs to enhance high yield and tolerance to field pests, including Maruca pod borers (<em>Maruca vitrata</em>) in locally adapted cowpea varieties. Seeds of two locally adapted cowpea genotypes (NkR1P3 and NamCp201) were treated with six gamma irradiation doses – 0 (un-irradiated), 75, 150, 300, 450, and 600 Gy. Consequently, the radio-sensitivity test was conducted in seed trays under net house conditions at the experimental field of Ogongo Campus, University of Namibia, Namibia. The study was arranged in a completely randomized design with three replications. Significant interactions (p &lt; 0.001) of genotype and gamma irradiation dosage were found on percentage emergence (%E) and seedling survival percentage (%SS). This revealed that optimum gamma irradiation doses for tested cowpea genotypes where genotypic and dose depended. The significant effect (p &lt; 0.001) of dose on all tested traits revealed the need to identify highly responsive traits for large-scale mutagenesis. Seedling survival rate and shoot length were the most responsive and were consequently used to determine the optimum dose for mutagenesis. The optimum dosage of gamma radiation for genotypes NkR1P3 and NamCp201 ranged between 382 and 427 Gy and 324 and 335 Gy, respectively. These optimal doses can be used to generate genetic variation to improve yield and tolerance to local stresses including tolerance to <em>M. vitrata</em> pod borers.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 2","pages":"Pages 83-87"},"PeriodicalIF":0.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000012/pdfft?md5=74aa402e67488ed649722b4338162069&pid=1-s2.0-S2667071224000012-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status and application of largemouth bass (Micropterus salmoides) germplasm resources","authors":"Pengfei Yu ,&nbsp;Hong Chen ,&nbsp;Mingli Liu ,&nbsp;Haitao Zhong ,&nbsp;Xueyan Wang ,&nbsp;Yilin Wu ,&nbsp;Yu Sun ,&nbsp;Chang Wu ,&nbsp;Shi Wang ,&nbsp;Chiye Zhao ,&nbsp;Chaoying Luo ,&nbsp;Chun Zhang ,&nbsp;Fangzhou Hu ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2024.01.004","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.01.004","url":null,"abstract":"<div><p>Largemouth bass (<em>Micropterus salmoides</em>, LB) is an important aquaculture and fishing species in the world. LB has been introduced into China since 1983, and its aquaculture production has increased year by year. The total yield of LB in China reached 802,486 tons in 2022, ranking seventh in China's freshwater fish aquaculture. However, the LB is facing threats such as degradation of germplasm resources and disease susceptibility due to limitations in the scale of introduction, coupled with the effects of high-density aquaculture, inbreeding, and species hybridization. This paper summarizes the current status of LB germplasm resources, variety genetic improvement, nutrition and fodder, aquaculture mode and diseases, and circulation and processing. This paper also provides recommendations on how to fully explore and utilize the existing germplasm resources of LB; culture new LB germplasm that combines excellent resistance and growth characteristics through innovations in breeding techniques; reduce the morbidity rate and improve the efficiency of cultured LB through the optimization of fodder formulations and innovation of aquaculture modes; and shift the processing of LB toward standardization, efficient nutrition and safety through the integration of high-tech and large-scale production. This paper provides a reference for the sustainable and healthy development of the LB industry.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 2","pages":"Pages 73-82"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000048/pdfft?md5=a977b9e1dfe2b41f037ea7da30d22877&pid=1-s2.0-S2667071224000048-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139674538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Lipin 1 polymorphisms for genetic markers in association with performance traits in Azikheli buffalo","authors":"Muhammad Siraj,&nbsp;Muhammad Ibrahim,&nbsp;Bibi Sabiha,&nbsp;Sohail Ahmad","doi":"10.1016/j.repbre.2024.01.003","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.01.003","url":null,"abstract":"<div><p><em>Lipin 1</em> regulates lipid metabolism in mammary glands. Genetic variation in <em>lipin 1</em> gene is thought to effect milk production in dairy animals. This study aimed to investigate the association of SNPs in the exon 6 and 8 of the <em>lipin 1</em> gene with milk yield in Azikheli buffalo from Swat, Pakistan. Azikheli buffaloes raised in a farm were selected based on their milk yield records. Three buffaloes were selected from the high milk yielding group with an average production of 12.96 ± 0.83 liters per day, and three from low yielding group producing 6.12 ± 0.28 liters of milk per day. Genomic DNA was extracted from the blood samples of these buffaloes, which were used to amplify exon 6, 8 the adjacent introns (6, 7 and 8) of the <em>lipin 1</em> gene. The PCR products was sequenced through sanger sequencing. A total of 45 SNPs were found in the targeted regions of the <em>lipin 1</em> gene in both high and low yielding buffaloes. Overall, four missense variants (g.62048T&gt;C; Met399Thr and g.62069C&gt;A; Pro406His) were found in the exon 8 of <em>lipin 1</em> gene in Azikheli buffalo. Milk yield was positive correlated with SNP g.62048T&gt;C; Met399Thr that was more frequent (0.83 vs 0.33) in high milk yielding buffaloes compared to the low yielding group. SNP g.62069C&gt;A; Pro406His was unique to low milk yielding buffaloes suggesting its negative association with milk yield. Moreover, the visualization of 3D structure of the <em>lipin I</em> protein constructed using Phyr2 web server and the Missense 3D analysis identified no structural damages caused by the reported SNPs on the target protein. In conclusion, SNPs g.62048T&gt;C; Met399Thr and g.62069C&gt;A; Pro406His could serve as potential genetic markers for increasing milk productivity in buffaloes.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 2","pages":"Pages 61-72"},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000036/pdfft?md5=851c43d2fb5938a0007b686514a65b69&pid=1-s2.0-S2667071224000036-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139653145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mtDNA copy number contributes to growth diversity in allopolyploid fish","authors":"Hong Zhang ,&nbsp;Mengxue Luo ,&nbsp;Yakui Tai ,&nbsp;Mengdan Li ,&nbsp;Jialin Cui ,&nbsp;Xin Gao ,&nbsp;Li Ren ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.12.008","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.008","url":null,"abstract":"<div><p>Phenotypic differences between diploid parents and their allotriploid offspring are common in aquaculture breeding. Some allotriploid populations exhibit rapid growth rates and increased body weight, which are significant for supporting fisheries development. Understanding the genetic mechanisms underlying these traits is crucial for implementing diverse breeding strategies to achieve high production in fish farming. Here, we collected the following fish species for our study: red crucian carp (<em>Carassius auratus</em> red var., 2nRR), common carp (<em>Cyprinus carpio</em> L., 2nCC), and two allotriploids (3nR₂C and 3 nRC₂). These allotriploids were obtained through backcrossing an allotetraploid of <em>C. auratus</em> red var. × <em>C. carpio</em> L. (4nR₂C₂, ♂) with <em>C. auratus</em> red var. and <em>C. carpio</em> L. (♀), respectively. These allotriploids demonstrated faster growth rates compared to their diploid inbred parents, contributing to the Chinese fisheries industry for several decades. We conducted a systematic comparison of mitochondrial DNA (mtDNA) copy numbers in the liver and muscle tissues of 2nRR, 2nCC, 3nR₂C, and 3 nRC₂ under different seasons. When entering winter (low water temperature: 13 °C), the triploid fish (3nR₂C and 3 nRC₂) exhibited lower mtDNA copy numbers in the muscle, indicating a reduction in individual activity and energy expenditure to facilitate weight maintenance when food availability is limited. Furthermore, we analysed the expression levels of three nuclear-regulated mitochondrial genes (<em>tfam</em>, <em>tfb1m</em>, and <em>tfb2m</em>) and observed an imbalance of allelic expression in <em>tfam</em> and <em>tfb1m</em> in the two triploid fish. These findings enhance our understanding of the molecular regulatory mechanisms underlying growth trait differences among fish with different ploidy levels.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 2","pages":"Pages 55-60"},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000637/pdfft?md5=70ef54ba64237d629176fdd8764a5458&pid=1-s2.0-S2667071223000637-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139653144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of allodiploid and allotriploid fish derived from hybridization between Cyprinus carpio haematopterus (♀) and Gobiocypris rarus (♂)","authors":"Min Wang ,&nbsp;Yuan Ou ,&nbsp;Zijian Guo ,&nbsp;Juan Li ,&nbsp;Huilin Li ,&nbsp;Xinyi Li ,&nbsp;Jingyang Li ,&nbsp;Shi Wang ,&nbsp;Qingfeng Liu ,&nbsp;Jing Wang ,&nbsp;Yuqin Shu ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.12.005","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.005","url":null,"abstract":"<div><p>The production of hybrid progeny through distant hybridization holds great significance in enriching germplasm resources for fish breeding. In this study, a hybridization experiment was conducted between female KOC (<em>Cyprinus carpio haematopterus</em>) and male GR (<em>Gobiocypris rarus</em>), resulting in the production of two distinct types of hybrid offspring. These progen<u>ies</u> were classified as allodiploid and allotriploid based on their DNA content and chromosome numbers, hereafter referred to as CG and CCG. Subsequently, a comprehensive comparative analysis was performed between the CG and CCG hybrids and their parents, focusing on countable traits, measurable traits, erythrocyte morphology, as well as karyogene and mitochondrial gene composition. The majority of the examined countable and measurable traits in both CG and CCG exhibited similarities predominantly with GR, except for the ratios of body length (BL) to body height (BH) and head length (HL). Moreover, observing erythrocytes revealed the presence of dumbbell-shaped nuclei in CCG, a characteristic not observed in CG hybrids or the parents. Sequencing alignment revealed that the <em>homeobox</em> (Hox) genes and 5S RNA in CG and CCG were inherited from both KOC and GR, signifying their status as allodiploid and allotriploid organisms. The mitochondrial genes in CG and CCG showed substantial similarity to KOC, albeit with a few sites displaying paternal leakage inheritance from GR. In comparison to CG, the growth rate of CCG was found to be significantly faster, which could be attributed to the upregulation of <em>growth hormone 1</em> (<em>gh1</em>) and the downregulation of <em>myostatin b</em> (<em>mstn</em>). This study successfully produced two hybrid offspring with distinct growth characteristics but similar genetic backgrounds, making them ideal subjects for future investigations into growth traits. The findings of this research established a fundamental basis for investigating the growth mechanism of fish and provided significant implications for the advancement of fish breeding through hybridization.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 46-54"},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000601/pdfft?md5=fe70c731b0e0b2c6284867dcab47e340&pid=1-s2.0-S2667071223000601-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139654163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful live births after Chinese herbal medicine treatment on a patient of advanced maternal age with severe diminished ovarian reserve: A case report","authors":"Xiaoxiong Shen","doi":"10.1016/j.repbre.2023.12.007","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.007","url":null,"abstract":"<div><p>The fecundity of women decreases significantly beginning approximately at age 35 and decreases more rapidly after age 40. Age affects not only the size of the oocyte pool but also the quality of germ cells. Despite the continuous advancement of assisted reproductive technology, infertility remains a challenge for women of advanced maternal age. Here, we present a case of a woman in her forties who had two successful live births after treatment with Traditional Chinese Medicine. At her initial visit (at age 43), the patient presented with a short menstrual cycle and signs of a diminished ovarian reserve (FSH: 26.2 mIU/mL; AMH: 0.23 ng/mL). Three years after her first delivery (at age 47), the patient showed signs of further diminished ovarian reserve (FSH: 27.1 mIU/mL; AMH: 0.07 ng/mL). Notably, after five months of Chinese herbal medicine treatment, she achieved another successful pregnancy, and resulted in a live birth. This case demonstrates that Chinese herbal medicine can have a positive clinical impact on improving hormonal balance and fecundity. Further research is needed to investigate the pharmacokinetics of Chinese herbal medicine in improving fecundity for women of advanced maternal age.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 32-37"},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000625/pdfft?md5=8dc2dfd6dc1b7096cfba41fe6ac01bdc&pid=1-s2.0-S2667071223000625-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139654160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"WbuB, a glycosyltransferase family 4 protein, regulates the activation of NLRP3 inflammasome and contributes to the virulence of Aeromonas hydrophila CCL1","authors":"Qiongyao Zeng ,&nbsp;Jiaxu Qiang ,&nbsp;Ye Yang ,&nbsp;Zhengwei Li ,&nbsp;Pingyuan Li ,&nbsp;Niewen Hu ,&nbsp;Zejun Zhou","doi":"10.1016/j.repbre.2023.12.006","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.006","url":null,"abstract":"<div><p><em>Aeromonas hydrophila</em> is an opportunistic pathogen of fishes and aquatic animals. <em>A</em>. <em>hydrophila</em> has a strong ability to disrupt gut integrity and cause inflammation and septicemia in fish, however, the mechanism is not fully understood. In this study, we identified the function of a galactosaminogalactan (GAG) synthase (named WbuB) in the pathogenic <em>A</em>. <em>hydrophila</em> CCL1. WbuB belongs to the family 4 of glycosyltransferases (GT4) and is composed of 407 amino acids (aa). For virulence analysis, the mutant which has an in-frame deletion of the <em>WbuB</em> gene in CCL1 was created (named Δ<em>WbuB</em>). Δ<em>WbuB</em> had the decreased biofilm formation, as well as adhesion ability and cytotoxicity. Animal infection study in crucian carps showed that, compared to CCL1, Δ<em>WbuB</em> caused the decreased expression levels of <em>ASC</em>, <em>NLRP3</em>, <em>caspase-1</em> and <em>IL-1β</em> in gut. Moreover, the expression levels of <em>ZO-1</em> and <em>Occludin</em> were increased by Δ<em>WbuB</em> infection. In line with the results, the intestinal permeability and tissue dissemination capacity of Δ<em>WbuB</em> were attenuated significantly. These lost virulence capacities of Δ<em>WbuB</em> were restored by complementation with the <em>WbuB</em> gene. Taken together, these results indicate that WbuB is essential for the activation of NLRP3 inflammasome and is a novel virulent factor for tight junction barrier during <em>A</em>. <em>hydrophila</em> infection.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 38-45"},"PeriodicalIF":0.0,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000613/pdfft?md5=8c5901d41635b89422a18248194db901&pid=1-s2.0-S2667071223000613-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139654140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the genetic characteristics and variations in disease-resistant grass carp based on whole-genome resequencing and transcriptome sequencing","authors":"Huifang Tan ,&nbsp;Yude Wang ,&nbsp;Biao Hu ,&nbsp;Yongjie Zhang ,&nbsp;An'min Liao ,&nbsp;Wuxia Liu ,&nbsp;Chen Gen ,&nbsp;Kaikun Luo ,&nbsp;Min Tao ,&nbsp;Chun Zhang ,&nbsp;Qinbo Qin ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.12.003","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.003","url":null,"abstract":"<div><p>Disease-resistant grass carp (DR-GGC) was obtained by backcrossing gynogenetic grass carp (GGC, 2n = 48, female) with common grass carp (<em>Ctenopharyngodon idellus</em> [GC], 2n = 48, male). The genetic constitution and the potential mutation associated with growth dominance in DR-GGC were analyzed by genomic <em>in situ</em> hybridization (GISH), whole-genome resequencing (WGRS) and RNA-sequencing (RNA-seq). The GISH results showed partial translocations and deletions in the chromosomes of DR-GGC compared with GC. The WGRS results demonstrated that there were numerous genetic variations between DR-GGC and its parents, among which DR-GGC had the highest genomic heterozygosity and GGC had the highest genomic purity. The RNA-seq results revealed that the differentially expressed genes (DEGs) were significantly enriched in cellular processes and immune pathways in DR-GGC and GGC compared with GC. Meanwhile, the combined analysis of WGRS and RNA-seq identified DEGs with overlapping mutation loci in DR-GGC and GGC compared with GC. These DEGs were significantly enriched in the pathways of growth, metabolism, and immunity, including cellular metabolism, biosynthesis, glycolysis/gluconeogenesis, and extra cellular matrix (ECM) receptor interactions. These results provide a genetic basis for exploring the better growth characteristics and disease resistance of DR-GGC than GC, valuable information on the genetic breeding for grass carp, and a further understanding of the genetic characteristics of gynogenetic individuals.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 22-31"},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000583/pdfft?md5=3cfba8b2e7625b06b221e67a37e35da2&pid=1-s2.0-S2667071223000583-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of nutrients in muscle and ovary between an improved fish and its parents","authors":"Xuanyi Zhang ,&nbsp;Fanglei Liu ,&nbsp;Bei Li ,&nbsp;Jianming Yu ,&nbsp;Lujiao Duan ,&nbsp;Ziyi Huang ,&nbsp;Zejun Zhou ,&nbsp;Yuqin Shu ,&nbsp;Jingjing Lin ,&nbsp;Xiaoxia Xiong ,&nbsp;Qingfeng Liu ,&nbsp;Shaojun Liu","doi":"10.1016/j.repbre.2023.12.002","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.002","url":null,"abstract":"<div><p>In previous studies, an allodiploid lineage (WR-F1-F5) was established by the distant hybridization of white crucian carp (<em>Carassius cuvieri</em>, WCC, ♀) and red crucian carp (<em>Carassius auratus</em> red var., RCC, ♂), an allodiploid fish (WR-II) was produced by WR-F1 (♀) × WCC (♂). In this study, the nutrients in the muscle and ovary of WR-II and its parents (WCC, RCC, and WR) were investigated, including protein, fat, amino acid, docosahexaenoic acid (DHA), moisture, and ash. The results showed that the protein content of WR-II in muscle and ovary (17.90 % and 25.60 % respectively) was higher than that of WR, and was significantly higher than that of WCC and RCC (<em>p</em> &lt; 0.05). The fat content of WR-II in muscle and ovary (4.30 % and 5.31 % respectively) was higher than that of WR and was significantly higher than that of WCC and RCC (<em>p</em> &lt; 0.05), and the muscle and ovary of WR-II showed rich DHA content (0.32 % and 0.54 % respectively) than that of WCC and RCC (<em>p</em> &lt; 0.05). Fifteen amino acids were detected in WR-II and its parents, the total amino acid (TAA) content of WR-II in muscle and ovary (15.49 % and 18.43 % respectively) was significantly higher than that of WCC (<em>p</em> &lt; 0.05), the delicious amino acid (DAA) content of WR-II in muscle and ovary (6.56 % and 8.29 % respectively) was different degrees higher than that of WCC, RCC, and WR. The moisture and carbohydrates of WR-II in muscle and ovary were lower than those in WCC and RCC. Furthermore, protein, fat, TAA, and DAA content in the ovary were significantly higher than those in muscle (<em>p</em> &lt; 0.05), showing tissue differences in all detected fish. This study indicated that WR-II had the characteristics of high protein, high fat, high DHA, high TAA, high DAA, low moisture, and low carbohydrates, which provided theoretical support for its application.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 16-21"},"PeriodicalIF":0.0,"publicationDate":"2023-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071223000571/pdfft?md5=45ada50f3d5631f1540302daf06fa187&pid=1-s2.0-S2667071223000571-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different color varieties of Asian arowana are unlikely distinct species: Molecular and reproductive evidence","authors":"Gen Hua Yue ,&nbsp;Alex Chang","doi":"10.1016/j.repbre.2023.12.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2023.12.001","url":null,"abstract":"<div><p>The Asian arowana (<em>Scleropages formosus</em>) has gained immense popularity in the ornamental fish trade due to its striking color variations. However, ongoing debates persist regarding the taxonomic classification of these distinct color varieties as separate species. This paper aims to address this issue by employing mtDNA sequencing, microsatellite genotyping, and crossing experiments. Phylogenic analyses using mitochondrial and nuclear markers across green, golden, and red varieties showed that individuals from these tree color varieties were mixed in several clusters. Crossing between different varieties generated fertile offspring. These data indicates that three color morphs of the Asian arowana should not be classified as distinct species. Acknowledging the aesthetic significance of these varieties, a holistic understanding of their taxonomic status is crucial for informed conservation strategies and sustainable management of this species.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 1","pages":"Pages 10-15"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266707122300056X/pdfft?md5=e88b849b51afb378fe96aa8a173d55d1&pid=1-s2.0-S266707122300056X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139033662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}