Philosophia (Ramat-Gan, Israel)最新文献_第9页

A novel human myelomonocytoid cell line, P39/Tsugane, derived from overt leukemia following myelodysplastic syndrome. 一种新的人骨髓单核细胞样细胞系P39/Tsugane，来源于骨髓增生异常综合征后的明显白血病。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1100

M. Nagai, S. Seki, T. Kitahara, T. Abe, K. Minato, S. Watanabe, M. Shimoyama

{"title":"A novel human myelomonocytoid cell line, P39/Tsugane, derived from overt leukemia following myelodysplastic syndrome.","authors":"M. Nagai, S. Seki, T. Kitahara, T. Abe, K. Minato, S. Watanabe, M. Shimoyama","doi":"10.20772/CANCERSCI1959.75.12_1100","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1100","url":null,"abstract":"A novel human cultured cell line, P39/Tsugane, was established from leukemic cells in the peripheral blood of a 69-year-old male with overt leukemia following myelodysplastic syndrome (MDS). P39/Tsugane cells were characterized by blastic appearance, presence of NaF-sensitive alpha-naphthyl butylate esterase activity, Fc gamma-receptor, C3-receptor, capacity to phagocytize sensitized erythrocytes, and reactivity with monoclonal antibodies such as OKT4, My4, VIMD5, MCS-2 and My7. These data indicate that P39/Tsugane cells are of myelomonocytoid nature. P39/Tsugane had a hypodiploid chromosome constitution with a gain of a consistent marker, 6q+, the presence of less consistent markers 9q+ and rcp(14;16), and random and non-random losses of autosomes: in accordance with the reported cytogenetic profiles of MDS, a representative karyotype of the present cell line is 45,XY,+del(6)(q15),9q+, t(14;16)-(q24;q21),-16,-17. P39/Tsugane cells were transplantable intraperitoneally into nude mice, and produced abdominal tumors and hemorrhagic ascites. These results indicate that P39/Tsugane is the first cultured cell line of myelomonocytoid nature to be derived from overt leukemia following MDS. Therefore, P39/Tsugane cells should be useful for studies on the differentiation of leukemia cells, the pathogenesis of MDS and in vitro-in vivo experimental chemotherapy.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"121 1","pages":"1100-7"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90575302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Interconversion of biological characteristics of small cell lung cancer depending on culture conditions. 小细胞肺癌生物学特性随培养条件的相互转化。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1089

T. Terasaki, T. Kameya, T. Nakajima, M. Tsumuraya, Y. Shimosato, K. Kato, H. Ichinose, T. Nagatsu, T. Hasegawa

{"title":"Interconversion of biological characteristics of small cell lung cancer depending on culture conditions.","authors":"T. Terasaki, T. Kameya, T. Nakajima, M. Tsumuraya, Y. Shimosato, K. Kato, H. Ichinose, T. Nagatsu, T. Hasegawa","doi":"10.20772/CANCERSCI1959.75.12_1089","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1089","url":null,"abstract":"Two distinct cell lines were obtained from a single heterotransplanted tumor which had originated from a primary focus of small cell carcinoma of the lung (SCCL). They were maintained separately from the beginning in culture media with and without fetal calf serum supplementation. Cells in the serum-free medium grew mostly floating in loose aggregates and showed poor cell cohesiveness, scanty cytoplasm and a few intracytoplasmic small dense-cored granules; all of these features are characteristics of oat cell type SCCL. On the other hand, cells in the serum-supplemented medium grew mostly floating in flatter and more closely associated clumps, were larger, and showed increased cell cohesiveness, occasional tubular structures, better developed organelles including dense-cored granules, and an increased number of cell attachments; these features are characteristics of intermediate cell type SCCL. The modal number of chromosomes differed from each other. Neuron-specific enolase (gamma enolase) and aromatic L-amino acid decarboxylase (ADC) activities in cell pellets were significantly higher in both lines than in control non-small cell lung cancer cell lines. The alpha/gamma type enolase ratio was lower, as was the ADC activity, in serum-free cultures than in serum-supplemented cultures. Interchange of the culture medium induced changes of the growth pattern and cell type from \"oat cell type\" to \"intermediate cell type\" and vice versa. The chromosomal number also partially changed. These findings suggest that cultured cells of SCCL alter their growth pattern and cell type depending on the culture conditions and that the selective growth of one cell type might then take place.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"16 1","pages":"1089-99"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86355302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Study of the promoting effect of sodium chloride on gastric carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine in inbred Wistar rats. 氯化钠促进n -甲基-n′-硝基-n -亚硝基胍致自交系Wistar大鼠胃癌的研究。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1053

H. Ohgaki, T. Kato, K. Morino, N. Matsukura, S. Sato, S. Takayama, T. Sugimura

引用次数: 15

Reversal of multidrug resistance by non-antitumor anthracycline analogs. 逆转非抗肿瘤蒽环类药物的多药耐药。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1049

Makoto Inaba, Kyoko Nagashima, Yoshio Sakurai, Masaru Fukui, Yoshikazu Yanagi

引用次数: 28

Sialidase of rat hepatomas: qualitative and quantitative comparison with rat liver sialidase. 大鼠肝癌唾液酸酯酶:与大鼠肝唾液酸酯酶的定性、定量比较。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1076

T. Miyagi, T. Goto, S. Tsuiki

{"title":"Sialidase of rat hepatomas: qualitative and quantitative comparison with rat liver sialidase.","authors":"T. Miyagi, T. Goto, S. Tsuiki","doi":"10.20772/CANCERSCI1959.75.12_1076","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1076","url":null,"abstract":"The subcellular distribution of sialidase in rat hepatoma induced by 3'-methyl-4-dimethylaminoazobenzene was studied by using sialyllactose as a substrate in the pH range of 4.0-7.0. As found in rat liver, the activity was recovered largely in the mitochondrial/lysosomal fraction with an optimal pH of 4.5 and in the cytosolic fraction with an optimal pH of 6.0, although hepatoma lysosomal (acidic) sialidase was also distributed in the microsomal fraction. The lysosomal and cytosolic sialidases of the hepatoma were indistinguishable from the corresponding enzymes of liver in chromatographic behavior, kinetics and substrate specificity. The levels of lysosomal and cytosolic sialidase activities in liver and hepatomas were then studied in the pellet and supernatant fractions, respectively, obtained by centrifuging the postnuclear supernatant at 105,000g for 1 hr. All the hepatomas tested, one primary and three transplanted, showed higher lysosomal sialidase and lower cytosolic sialidase activities as compared with liver. Quantitative changes similar to those seen in hepatomas were observed in regenerating liver after partial hepatectomy.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"66 1","pages":"1076-82"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86791570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Suppressive effect of cupric acetate on DNA alkylation, DNA synthesis and tumorigenesis in the liver of dimethylnitrosamine-treated rats. 醋酸铜对二甲基亚硝胺处理大鼠肝脏DNA烷基化、DNA合成及肿瘤发生的抑制作用。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1062

Y. Yamane, K. Sakai, T. Umeda, N. Murata, S. Ishizeki, I. Ogihara, A. Takahashi, I. Iwasaki, G. Ide

{"title":"Suppressive effect of cupric acetate on DNA alkylation, DNA synthesis and tumorigenesis in the liver of dimethylnitrosamine-treated rats.","authors":"Y. Yamane, K. Sakai, T. Umeda, N. Murata, S. Ishizeki, I. Ogihara, A. Takahashi, I. Iwasaki, G. Ide","doi":"10.20772/CANCERSCI1959.75.12_1062","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1062","url":null,"abstract":"The effect of cupric acetate on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in rats was investigated. The surviving rats in the group given DMN (25 ppm) in the drinking water alone were killed at 26 weeks and it was found that 12 of 16 rats had developed liver tumors. In the group given DMN and cupric acetate (sc injections of 2 mg of Cu/kg of body weight once a week for 26 weeks), 7 of 22 rats developed liver tumors. The incidence of liver tumors in rats given DMN and cupric acetate was thus only about 40% of that in rats given DMN alone. No tumor was observed in the group given saline or cupric acetate alone. The thymidine incorporation into the liver DNA of rats was measured at 2 and 4 weeks after the start of the carcinogenicity experiment. The thymidine incorporation into the liver DNA of rats given DMN was significantly suppressed by the administration of cupric acetate. The methylation of liver DNA in rats given a single dose of DMN was also significantly suppressed by sc injection of cupric acetate; the formation of both O6-methylguanine and 7-methylguanine was reduced. This result suggests that sc injection of cupric acetate may have a suppressive effect on the initiation of carcinogenesis in the liver.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"92 1","pages":"1062-9"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86972900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Transfusion-mediated spread of the human T-cell leukemia virus in chronic hemodialysis patients in a heavily endemic area, Nagasaki. 在长崎严重流行地区慢性血液透析患者中输血介导的人t细胞白血病病毒传播

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1070

S. Hino, T. Kawamichi, M. Funakoshi, M. Kanamura, T. Kitamura, T. Miyamoto

引用次数: 21

The capacity of antigen-presenting cells is fully preserved in childhood cancer patients. 抗原提呈细胞的能力在儿童癌症患者中得到充分保存。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1108

Y. Koide, T. Hongo, R. Iseki, Y. Mori, T. Yoshida

{"title":"The capacity of antigen-presenting cells is fully preserved in childhood cancer patients.","authors":"Y. Koide, T. Hongo, R. Iseki, Y. Mori, T. Yoshida","doi":"10.20772/CANCERSCI1959.75.12_1108","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1108","url":null,"abstract":"T cells from 19 out of 25 childhood cancer patients showed impaired proliferative responses to purified protein derivatives (PPD)-pulsed antigen-presenting cells (APC) although all of the patients had been immunized with BCG. To test whether such low responsiveness of T cells results from the dysfunction of T cells or from that of APC, the experiment was designed to assess the proliferative response of T cells from patients or their parents to PPD-pulsed APC from patients or parents. These combinations seem to be suitable to assess the activity of T cells or APC since at least partial identity of HLA-D/DR antigens is required for T cell-APC interactions. Although T cells from patients who showed low responsiveness to PPD failed to respond even to PPD-pulsed APC from parents, T cells from parents were able to respond to PPD-pulsed APC from patients as well as to autologous APC. These observations strongly suggest that the low responsiveness to PPD in childhood cancer patients results from the dysfunction of T cells, and the capacity of APC is fully preserved. In other words, it appears that the capacity of APC is not impaired by chemotherapy, neoplastic cells, or other factors. Suppressor T cells appeared not to be involved in such dysfunction of T cells.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"18 1","pages":"1108-15"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83800448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Suppressive effect of caffeine on the development of hepatic tumors induced by 2-acetylaminofluorene in ACI rats. 咖啡因对2-乙酰氨基芴致ACI大鼠肝脏肿瘤发展的抑制作用。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-12-01 DOI: 10.20772/CANCERSCI1959.75.12_1058

S. Hosaka, H. Nagayama, I. Hirono

引用次数: 10

Single cell origin of radiation-induced thymic lymphoma in mice with cellular mosaicism. 细胞嵌合体小鼠胸腺淋巴瘤的单细胞起源。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_1002

M. Bessho, I. Jinnai, K. Hirashima, K. Tanaka, H. Tanooka

引用次数: 9