Philosophia (Ramat-Gan, Israel)最新文献_第10页

Cytotoxicity of autologous and allogeneic lymphocytes against cultured human lung cancer cells: optimal conditions for the production of cytotoxic lymphocytes. 自体和异体淋巴细胞对培养的人肺癌细胞的细胞毒性:产生细胞毒性淋巴细胞的最佳条件。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_1006

H. Kimura, Y. Yamaguchi, T. Fujisawa

{"title":"Cytotoxicity of autologous and allogeneic lymphocytes against cultured human lung cancer cells: optimal conditions for the production of cytotoxic lymphocytes.","authors":"H. Kimura, Y. Yamaguchi, T. Fujisawa","doi":"10.20772/CANCERSCI1959.75.11_1006","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_1006","url":null,"abstract":"Optimal conditions for the in vitro production of cytotoxic lymphocytes against autologous lung cancer cells were studied. In the time-course experiments, fresh lymphocytes did not exhibit any cytotoxicity against autologous tumor cells, but, when cultured in the presence of T cell growth factor (IL2), the lymphocytes became cytotoxic against autologous tumor cells 3 days after the initiation of incubation and the cytotoxicity continued to increase, reaching a maximum at day 7. The study, conducted to compare the effects of IL2 and phytohemagglutinin (PHA), demonstrated that although lymphocytes cultured with PHA did exhibit a significant cytotoxicity, lymphocytes cultured with IL2 showed much higher activity. Peripheral blood, regional lymph node (RNL) and distal lymph node lymphocytes, and tumor infiltrating lymphocytes were tested as sources for the production of cytotoxic lymphocytes. Among these 4 sources, RNL proved the most consistently reliable source of cytotoxic lymphocytes. The results of in vitro stimulation by autologous tumor cells were ambivalent. Twenty-five experiments that compared in vitro stimulation by tumor cells and no stimulation revealed that 9 cases (36%) showed enhancement of cytotoxicity after stimulation with tumor cells, 5 cases showed inhibition, and the remainder no effect. Characterization of lymphocytes by using monoclonal antibodies indicated that cells lytic for autologous tumor cells are OKT3+, OKT8+, OKT4-, OKM1-.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"3 1","pages":"1006-16"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87166330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

A new strain of Epstein-Barr virus derived from nasopharyngeal carcinoma hybrid cells. 从鼻咽癌杂交细胞中分离得到一株新的eb病毒。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_947

T. Takimoto, H. Ogura, H. Sato, M. Hatano

引用次数: 9

Summation and synergism in the promotion of urinary bladder carcinogenesis initiated by N-butyl-N-(4-hydroxybutyl)-nitrosamine in F344 rats. n -丁基-n -(4-羟基丁基)-亚硝胺对F344大鼠膀胱癌促进作用的总结与协同作用。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_950

T. Sakata, T. Shirai, Shoji Fukushima, R. Hasegawa, N. Ito

{"title":"Summation and synergism in the promotion of urinary bladder carcinogenesis initiated by N-butyl-N-(4-hydroxybutyl)-nitrosamine in F344 rats.","authors":"T. Sakata, T. Shirai, Shoji Fukushima, R. Hasegawa, N. Ito","doi":"10.20772/CANCERSCI1959.75.11_950","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_950","url":null,"abstract":"Summation and synergism in the effects of three tumor promoters on urinary bladder carcinogenesis initiated by a 4-week treatment with 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) in male F344 rats were examined. In experiment 1, the sequential administration of sodium saccharin (SS, 5.0%), DL-tryptophan (Tr, 2.0%) and sodium L-ascorbate (SA, 5.0%) in the diet, each for 10 weeks, significantly increased the incidence and the number of bladder tumors over that observed after SS alone or SS followed by Tr. In experiment 2, the simultaneous dietary administration of 2.5% SA, 1.0% butylated hydroxyanisole and 0.01% allopurinol for 32 weeks significantly increased the yield of bladder tumors. Paired combinations of promoters or each of the promoters administered alone were associated with a less pronounced promotive effect than when all three were combined. Thus, it is evident from the results of the present investigation that whatever the mechanisms underlying promotion by the different agents, they are capable of working in an additive fashion, under conditions of summation (consecutive administration) or synergism (simultaneous administration).","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"2 1","pages":"950-6"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79563216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Correlation between drug sensitivity determined by clonogenic cell assay and clinical effect of chemotherapy in patients with primary lung cancer. 原发肺癌患者克隆细胞测定药物敏感性与化疗临床效果的相关性研究。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_1030

E. Shimizu, N. Saijo, F. Kanzawa, A. Hoshi, K. Eguchi, T. Shinkai, K. Tominaga, Y. Sasaki, J. Fujita, H. Nomori

{"title":"Correlation between drug sensitivity determined by clonogenic cell assay and clinical effect of chemotherapy in patients with primary lung cancer.","authors":"E. Shimizu, N. Saijo, F. Kanzawa, A. Hoshi, K. Eguchi, T. Shinkai, K. Tominaga, Y. Sasaki, J. Fujita, H. Nomori","doi":"10.20772/CANCERSCI1959.75.11_1030","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_1030","url":null,"abstract":"Correlation studies between the sensitivity of tumor cells determined by clonogenic cell assay and the clinical effect of chemotherapy were performed in patients with primary lung cancer. Thirty-four of 48 patients showed adequate colony formation (greater than or equal to 30 colonies) to test in vitro chemosensitivity. Colony formation of tumor specimens did not differ with regards to prognostic factors such as sex, age, stage, performance status, and prior chemotherapy. The two criteria of in vitro chemosensitivity employed were greater than 70% and 50% reduction in colony numbers. When in vitro chemosensitivity was defined as a colony reduction of greater than 50%, the true positive rate in the assay was 57%, while the true negative rate was 85%. In this case, the predictive accuracy was 79%. When in vitro chemosensitivity was defined as a colony reduction of greater than 70%, the true positive rate for the assay was 100%, while the true negative rate was 81%. In this case, the predictive accuracy was 82%. By Fisher's exact probability test, the overall in vitro/in vivo correlation was statistically significant (P = 0.042 less than 0.05) with the 50% cut-off point, but was not significant (P = 0.053 greater than 0.05) with the 70% cut-off point.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"31 1","pages":"1030-5"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74775211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Stimulation of human and murine bone marrow cell colony formation by colony-stimulating factors obtained from the urine of mice bearing leukocytosis-inducing fibrosarcoma. 从白细胞诱导性纤维肉瘤小鼠尿液中获得的集落刺激因子刺激人和小鼠骨髓细胞集落形成。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_993

M. Bessho, M. Shikita, K. Tsuneoka, N. Sakai, K. Hirashima

{"title":"Stimulation of human and murine bone marrow cell colony formation by colony-stimulating factors obtained from the urine of mice bearing leukocytosis-inducing fibrosarcoma.","authors":"M. Bessho, M. Shikita, K. Tsuneoka, N. Sakai, K. Hirashima","doi":"10.20772/CANCERSCI1959.75.11_993","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_993","url":null,"abstract":"Dialyzed urine of mice bearing leukocytosis-inducing fibrosarcoma stimulated granulocyte colony formation in semisolid agar culture of human bone marrow cells. Removal of phagocytic cells prior to stimulation did not interfere with the formation of these colonies in the culture. On the other hand, macrophage colonies were predominantly produced when murine bone marrow cells were stimulated by the dialyzed mouse urine. The activity of colony-stimulating factor (CSF) in the urine of normal mice was less than 1/100 of that in the urine of tumor-bearing mice. DEAE-cellulose column chromatography separated the activity stimulating human granulocyte colony formation from that stimulating murine macrophage colony formation. Further purification showed that a sialoglycoprotein with an apparent molecular weight of 80,000 corresponded to the macrophage CSF, which was devoid of activity toward human cells. The molecular properties of the human-active granulocyte CSF could not be studied further, because it was quite unstable.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"85 1","pages":"993-1001"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83262402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Stable intestinal phenotypic expression of gastric and small intestinal tumor cells induced by N-methyl-N'-nitro-N-nitrosoguanidine or methylnitrosourea in rats. n -甲基-n '-硝基-n -亚硝基胍或甲基亚硝基脲诱导大鼠胃和小肠肿瘤细胞的稳定肠道表型表达

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_957

M. Tatematsu, T. Katsuyama, C. Furihata, H. Tsuda, N. Ito

{"title":"Stable intestinal phenotypic expression of gastric and small intestinal tumor cells induced by N-methyl-N'-nitro-N-nitrosoguanidine or methylnitrosourea in rats.","authors":"M. Tatematsu, T. Katsuyama, C. Furihata, H. Tsuda, N. Ito","doi":"10.20772/CANCERSCI1959.75.11_957","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_957","url":null,"abstract":"On the basis of paradoxical concanavalin A (Con A) staining, the tendency of tumor cells of gastric phenotype to shift to intestinal phenotype and the stability of the latter phenotype in stomach tumors of different sizes were examined quantitatively with an image processor. Phenotypic expression of tumors of the small intestine was also studied. One hundred male Wistar rats were given N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) at 50 micrograms/ml in their drinking water for 20 weeks (group 1). Twenty male F344 rats were given methylnitrosourea (MNU) at a dose of 50 mg/kg ip twice a week for 2 weeks (group 2). Rats in group 1 were killed in week 50 of the experiment and rats in group 2 were killed in week 25. In group 1, the percentage areas of intestinal-type cells in small, medium and large adenocarcinoma of the stomach were 0.5, 2.7 and 6.6%, the differences between these values being significant (P less than 0.05-0.01). Intestinal phenotypic expression of tumor cells of the stomach is stable and the proportion of intestinal-type cells in adenocarcinomas of the stomach is higher in the larger tumors. Adenomatous hyperplasias and adenocarcinomas of the small intestine in groups 1 and 2 were all composed entirely of cells of the intestinal type. These results suggested that intestinal-type cells in adenocarcinoma of the stomach did not originate from intestinal metaplasias but from gastric-type cells in stomach adenocarcinomas.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"42 1","pages":"957-65"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75722436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Tumor-associated expression of glycosphingolipid Hanganutziu-Deicher antigen in human cancers. 糖鞘脂Hanganutziu-Deicher抗原在人类癌症中的肿瘤相关表达

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_1025

H. Higashi, Y. Nishi, Y. Fukui, K. Ikuta, S. Ueda, S. Kato, M. Fujita, Y. Nakano, T. Taguchi, S. Sakai

{"title":"Tumor-associated expression of glycosphingolipid Hanganutziu-Deicher antigen in human cancers.","authors":"H. Higashi, Y. Nishi, Y. Fukui, K. Ikuta, S. Ueda, S. Kato, M. Fujita, Y. Nakano, T. Taguchi, S. Sakai","doi":"10.20772/CANCERSCI1959.75.11_1025","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_1025","url":null,"abstract":"Expression of heterophile Hanganutziu-Deicher (HD) antigen on the cell surface of various human malignant tumor tissues was studied by membrane immunofluorescence staining with chicken antiserum against N-glycolylneuraminyllactosylceramide (HD3) and fluorescein-conjugated rabbit anti-chicken IgG. The HD antigen was demonstrated in samples from 38 of 77 (38/77, 49%) cases, consisting of gastric (9/16), breast (8/14), colorectal (3/12), nasopharyngeal (4/7), and uterine (2/3) carcinomas, leukemias (5/10), malignant lymphomas (2/5), and other cancers (5/10). Histological examination indicated that expression of the antigen by the gastric, colorectal and breast carcinomas and leukemias was not related to their histological types. The cytoplasm and the surface of the malignant glandular epithelial cells were both specifically stained by immunofluorescence staining of frozen sections in one colon adenocarcinoma. Glycosphingolipids (GSLs) were extracted from the colon cancer tissue and two HD antigen-active GSLs were detected on thin-layer chromatography (TLC) by enzyme-immunostaining using affinity-purified chicken anti-HD3 and horseradish peroxidase-conjugated rabbit anti-chicken IgG. One migrated to the same position as HD3 on TLC, and the other to a position similar to that of authentic N-glycolylneuraminylneolactotetraosylceramide.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"4 1","pages":"1025-9"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75497324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Species difference between rats and mice in activities of enzymes activating aromatic amines: effect of dietary 3-methoxy-4-aminoazobenzene. 大鼠和小鼠活化芳香胺酶活性的物种差异:饲粮3-甲氧基-4-氨基偶氮苯的影响。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_966

M. Degawa, M. Kojima, Y. Hashimoto

引用次数: 16

Biomimetic preparation and structure determination of QGI, one of the quinoline-DNA base adducts formed in cells treated with 4-nitroquinoline 1-oxide. 喹啉- dna碱基加合物QGI的仿生制备及结构测定。QGI是一种在4-硝基喹啉- 1-氧化物处理的细胞中形成的碱基加合物。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-11-01 DOI: 10.20772/CANCERSCI1959.75.11_976

M. Tada, K. Kohda, Y. Kawazoe

引用次数: 18

DNA damage induced by asbestos in the presence of hydrogen peroxide. 过氧化氢存在下石棉引起的DNA损伤。

Philosophia (Ramat-Gan, Israel) Pub Date : 1984-10-01 DOI: 10.20772/CANCERSCI1959.75.10_841

H. Kasai, S. Nishimura

引用次数: 96