Oxford open neuroscience最新文献_第2页

Effects of optogenetic silencing the anterior cingulate cortex in a delayed non-match to trajectory task. 在延迟非匹配轨迹任务中光遗传学沉默前扣带回皮层的效果。

Oxford open neuroscience Pub Date : 2024-02-08 eCollection Date: 2024-01-01 DOI: 10.1093/oons/kvae002

Ana S Cruz, Sara Cruz, Miguel Remondes

{"title":"Effects of optogenetic silencing the anterior cingulate cortex in a delayed non-match to trajectory task.","authors":"Ana S Cruz, Sara Cruz, Miguel Remondes","doi":"10.1093/oons/kvae002","DOIUrl":"https://doi.org/10.1093/oons/kvae002","url":null,"abstract":"Working memory is a fundamental cognitive ability, allowing us to keep information in memory for the time needed to perform a given task. A complex neural circuit fulfills these functions, among which is the anterior cingulate cortex (CG). Functionally and anatomically connected to the medial prefrontal, retrosplenial, midcingulate and hippocampus, as well as motor cortices, CG has been implicated in retrieving appropriate information when needed to select and control appropriate behavior. The role of cingulate cortex in working memory-guided behaviors remains unclear due to the lack of studies reversibly interfering with its activity during specific epochs of working memory. We used eNpHR3.0 to silence cingulate neurons while animals perform a standard delayed non-match to trajectory task, and found that, while not causing an absolute impairment in working memory, silencing cingulate neurons during retrieval decreases the mean performance if compared to silencing during encoding. Such retrieval-associated changes are accompanied by longer delays observed when light is delivered to control animals, when compared to eNpHR3.0+ ones, consistent with an adaptive recruitment of additional cognitive resources.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"3 ","pages":"kvae002"},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10939314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRDM16 co-operates with LHX2 to shape the human brain. PRDM16 与 LHX2 共同塑造了人类大脑。

Oxford open neuroscience Pub Date : 2024-01-24 eCollection Date: 2024-01-01 DOI: 10.1093/oons/kvae001

Varun Suresh, Bidisha Bhattacharya, Rami Yair Tshuva, Miri Danan Gotthold, Tsviya Olender, Mahima Bose, Saurabh J Pradhan, Bruria Ben Zeev, Richard Scott Smith, Shubha Tole, Sanjeev Galande, Corey C Harwell, José-Manuel Baizabal, Orly Reiner

{"title":"PRDM16 co-operates with LHX2 to shape the human brain.","authors":"Varun Suresh, Bidisha Bhattacharya, Rami Yair Tshuva, Miri Danan Gotthold, Tsviya Olender, Mahima Bose, Saurabh J Pradhan, Bruria Ben Zeev, Richard Scott Smith, Shubha Tole, Sanjeev Galande, Corey C Harwell, José-Manuel Baizabal, Orly Reiner","doi":"10.1093/oons/kvae001","DOIUrl":"10.1093/oons/kvae001","url":null,"abstract":"PRDM16 is a dynamic transcriptional regulator of various stem cell niches, including adipocytic, hematopoietic, cardiac progenitors, and neural stem cells. PRDM16 has been suggested to contribute to 1p36 deletion syndrome, one of the most prevalent subtelomeric microdeletion syndromes. We report a patient with a de novo nonsense mutation in the PRDM16 coding sequence, accompanied by lissencephaly and microcephaly features. Human stem cells were genetically modified to mimic this mutation, generating cortical organoids that exhibited altered cell cycle dynamics. RNA sequencing of cortical organoids at day 32 unveiled changes in cell adhesion and WNT-signaling pathways. ChIP-seq of PRDM16 identified binding sites in postmortem human fetal cortex, indicating the conservation of PRDM16 binding to developmental genes in mice and humans, potentially at enhancer sites. A shared motif between PRDM16 and LHX2 was identified and further examined through comparison with LHX2 ChIP-seq data from mice. These results suggested a collaborative partnership between PRDM16 and LHX2 in regulating a common set of genes and pathways in cortical radial glia cells, possibly via their synergistic involvement in cortical development.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"3 ","pages":"kvae001"},"PeriodicalIF":0.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining wearable fNIRS and immersive virtual reality to study preschoolers' social development: a proof-of-principle study on preschoolers' social preference. 结合可穿戴 fNIRS 和沉浸式虚拟现实技术研究学龄前儿童的社交发展：关于学龄前儿童社交偏好的原理验证研究。

Oxford open neuroscience Pub Date : 2023-12-15 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad012

Chiara Bulgarelli, Paola Pinti, Nadine Aburumman, Emily J H Jones

{"title":"Combining wearable fNIRS and immersive virtual reality to study preschoolers' social development: a proof-of-principle study on preschoolers' social preference.","authors":"Chiara Bulgarelli, Paola Pinti, Nadine Aburumman, Emily J H Jones","doi":"10.1093/oons/kvad012","DOIUrl":"10.1093/oons/kvad012","url":null,"abstract":"A child's social world is complex and rich, but has traditionally been assessed with conventional experiments where children are presented with repeated stimuli on a screen. These assessments are impoverished relative to the dynamics of social interactions in real life, and can be challenging to implement with preschoolers, who struggle to comply with strict lab rules. The current work meets the need to develop new platforms to assess preschoolers' social development, by presenting a unique virtual-reality set-up combined with wearable functional near-infrared spectroscopy (fNIRS). As a proof-of-principle, we validated this platform by measuring brain activity during self-guided social interaction in 3-to-5-year-olds, which is under-investigated, yet crucial to understand the basis of social interactions in preschoolers. 37 preschoolers chose an interaction partner from one of 4 human-like avatars of different gender and age. We recorded spontaneous brain fluctuations from the frontal and temporoparietal regions (notably engaged in social-categorization and preference) while children played a bubble-popping game with a preferred and an assigned avatar. 60% of the participants chose to play with the same-gender and same-age avatar. However, this result was driven by females (>80% vs. 50% in males). Different fronto-temporoparietal connectivity patterns when playing with the two avatars were observed, especially in females. We showed the feasibility of using a novel set-up to naturalistically assess social preference in preschoolers, which was assessed at the behavioural and functional connectivity level. This work provides a first proof-of-principle for using cutting-edge technologies and naturalistic experiments to study social development, opening new avenues of research.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"2 ","pages":"kvad012"},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing Spatial Working Memory in Pigs using an Automated T-Maze 用自动t形迷宫测试猪的空间工作记忆

Oxford open neuroscience Pub Date : 2023-10-04 DOI: 10.1093/oons/kvad010

L M Allen, D A Murphy, V Roldan, M N Moussa, A Draper, A Delgado, M Aguiar, M A Capote, T J J Jarome, K Lee, A T Mattfeld, R Prather, T A Allen

{"title":"Testing Spatial Working Memory in Pigs using an Automated T-Maze","authors":"L M Allen, D A Murphy, V Roldan, M N Moussa, A Draper, A Delgado, M Aguiar, M A Capote, T J J Jarome, K Lee, A T Mattfeld, R Prather, T A Allen","doi":"10.1093/oons/kvad010","DOIUrl":"https://doi.org/10.1093/oons/kvad010","url":null,"abstract":"Abstract Pigs are an important large animal model for translational clinical research but underutilized in behavioral neuroscience. This is due, in part, to a lack of rigorous neurocognitive assessments for pigs. Here, we developed a new automated T-maze for pigs that takes advantage of their natural tendency to alternate. The T-maze has obvious cross-species value having served as a foundation for cognitive theories across species. The maze (17’ x 13’) was constructed typically and automated with flanking corridors, guillotine doors, cameras, and reward dispensers. We ran nine pigs in (1) a simple alternation task and (2) a delayed spatial alternation task. Our assessment focused on the delayed spatial alternation task which forced pigs to wait for random delays (5, 60, 120, and 240sec) and burdened spatial working memory. We also looked at self-paced trial latencies, error types, and coordinate-based video tracking. We found pigs naturally alternated but performance declined steeply across delays (R2 = 0.84). Self-paced delays had no effect on performance suggestive of an active interference model of working memory. Positional and head direction data could differentiate subsequent turns on short but not long delays. Performance levels were stable over weeks in diverse strains and sexes, and thus provide a benchmark for future neurocognitive assessments in pigs.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"308 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135644370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Midbrain organoids-development and applications in Parkinson's disease. 中脑类器官——帕金森病的发展和应用

Oxford open neuroscience Pub Date : 2023-08-18 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad009

Hilary S Y Toh, Xin Yi Choo, Alfred Xuyang Sun

引用次数: 0

Glioblastoma modeling with 3D organoids: progress and challenges. 胶质母细胞瘤三维类器官建模:进展和挑战

Oxford open neuroscience Pub Date : 2023-07-06 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad008

Xin Wang, Yusha Sun, Daniel Y Zhang, Guo-Li Ming, Hongjun Song

{"title":"Glioblastoma modeling with 3D organoids: progress and challenges.","authors":"Xin Wang, Yusha Sun, Daniel Y Zhang, Guo-Li Ming, Hongjun Song","doi":"10.1093/oons/kvad008","DOIUrl":"10.1093/oons/kvad008","url":null,"abstract":"Glioblastoma (GBM) is the most aggressive adult primary brain tumor with nearly universal treatment resistance and recurrence. The mainstay of therapy remains maximal safe surgical resection followed by concurrent radiation therapy and temozolomide chemotherapy. Despite intensive investigation, alternative treatment options, such as immunotherapy or targeted molecular therapy, have yielded limited success to achieve long-term remission. This difficulty is partly due to the lack of pre-clinical models that fully recapitulate the intratumoral and intertumoral heterogeneity of GBM and the complex tumor microenvironment. Recently, GBM 3D organoids originating from resected patient tumors, genetic manipulation of induced pluripotent stem cell (iPSC)-derived brain organoids and bio-printing or fusion with non-malignant tissues have emerged as novel culture systems to portray the biology of GBM. Here, we highlight several methodologies for generating GBM organoids and discuss insights gained using such organoid models compared to classic modeling approaches using cell lines and xenografts. We also outline limitations of current GBM 3D organoids, most notably the difficulty retaining the tumor microenvironment, and discuss current efforts for improvements. Finally, we propose potential applications of organoid models for a deeper mechanistic understanding of GBM and therapeutic development.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"1 1","pages":"kvad008"},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41397397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interpersonal brain synchronization during face-to-face economic exchange between acquainted dyads. 熟人之间面对面经济交流时的人际大脑同步。

Oxford open neuroscience Pub Date : 2023-06-21 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad007

Yuto Kikuchi, Kensuke Tanioka, Tomoyuki Hiroyasu, Satoru Hiwa

{"title":"Interpersonal brain synchronization during face-to-face economic exchange between acquainted dyads.","authors":"Yuto Kikuchi, Kensuke Tanioka, Tomoyuki Hiroyasu, Satoru Hiwa","doi":"10.1093/oons/kvad007","DOIUrl":"https://doi.org/10.1093/oons/kvad007","url":null,"abstract":"Interpersonal brain synchronization (IBS) has been observed during social interactions and involves various factors, such as familiarity with the partner and type of social activity. A previous study has shown that face-to-face (FF) interactions in pairs of strangers increase IBS. However, it is unclear whether this can be observed when the nature of the interacting partners is different. Herein, we aimed to extend these findings to pairs of acquaintances. Neural activity in the frontal and temporal regions was recorded using functional near-infrared spectroscopy hyperscanning. Participants played an ultimatum game that required virtual economic exchange in two experimental settings: face-to-face and face-blocked conditions. Random pair analysis confirmed whether IBS was induced by social interaction. Contrary to the aforementioned study, our results did not show any cooperative behavior or task-induced IBS increase. Conversely, the random pair analysis results revealed that the pair-specific IBS was significant only in the task condition at the left and right superior frontal, middle frontal, orbital superior frontal, right superior temporal, precentral and postcentral gyri. Our results tentatively suggested that FF interaction in acquainted pairs did not increase IBS and supported the idea that IBS is affected by 'with whom we interact and how'.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":"2 ","pages":"kvad007"},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913825/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140869677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy metabolic pathways in neuronal development and function. 神经元发育和功能中的能量代谢途径

Oxford open neuroscience Pub Date : 2023-03-21 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad004

Sebastian Rumpf, Neeraja Sanal, Marco Marzano

引用次数: 0

Competing contextual processes rely on the infralimbic and prelimbic medial prefrontal cortices in the rat. 竞争上下文过程依赖于大鼠边缘下和边缘前内侧前额叶皮层

Oxford open neuroscience Pub Date : 2023-03-17 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad003

David N George, Simon Killcross, Josephine E Haddon

{"title":"Competing contextual processes rely on the infralimbic and prelimbic medial prefrontal cortices in the rat.","authors":"David N George, Simon Killcross, Josephine E Haddon","doi":"10.1093/oons/kvad003","DOIUrl":"10.1093/oons/kvad003","url":null,"abstract":"Ambiguous relationships between events may be established using interference procedures such as latent inhibition, extinction or counterconditioning. Under these conditions, the retrieval of individual associations between a stimulus and outcome is affected by contextual cues. To examine the roles of the dorsal (prelimbic) and ventral (infralimbic) medial prefrontal cortex in the contextual modulation of such associations, we investigated the context specificity of latent inhibition. Male Lister hooded rats were pre-exposed to two separate stimuli, one in each of two distinct contexts. Both stimuli were then paired with the delivery of mild foot-shock in the same one of these contexts. Finally, the strength of the resultant conditioned emotional response (CER) to each stimulus was assessed in each context. For the sham-operated control rats, the CER was attenuated for each stimulus when it was tested in the context in which it had been pre-exposed. Rats who had received lesions to the infralimbic cortex showed this effect only in the conditioning context, whereas rats with lesions to the prelimbic cortex showed the effect only in the context in which conditioning had not taken place. These findings indicate that infralimbic and prelimbic cortices play distinct, and competing, roles in the contextual modulation of initial and later learning.","PeriodicalId":74386,"journal":{"name":"Oxford open neuroscience","volume":" ","pages":"kvad003"},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10913818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49157459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic modifiers of synucleinopathies-lessons from experimental models. 同核病的遗传修饰因子——实验模型的经验教训

Oxford open neuroscience Pub Date : 2023-03-09 eCollection Date: 2023-01-01 DOI: 10.1093/oons/kvad001

Rachel Min Qi Lee, Tong-Wey Koh

引用次数: 0