Nature cardiovascular research最新文献_第3页

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00624-2

Peter Libby, Michael C Honigberg, Amélie Vromman

引用次数: 0

Mapping the transcriptional and epigenetic landscape of organotypic endothelial diversity in the developing and adult mouse. 在发育和成年小鼠中绘制器官型内皮多样性的转录和表观遗传景观。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 Epub Date: 2025-03-17 DOI: 10.1038/s44161-025-00618-0

Manuel E Cantu Gutierrez, Matthew C Hill, Gabrielle E Largoza, William B Gillespie, James F Martin, Joshua D Wythe

{"title":"Mapping the transcriptional and epigenetic landscape of organotypic endothelial diversity in the developing and adult mouse.","authors":"Manuel E Cantu Gutierrez, Matthew C Hill, Gabrielle E Largoza, William B Gillespie, James F Martin, Joshua D Wythe","doi":"10.1038/s44161-025-00618-0","DOIUrl":"10.1038/s44161-025-00618-0","url":null,"abstract":"The vascular endothelium features unique molecular and functional properties across different vessel types, such as between arteries, veins and capillaries, as well as between different organs, such as the leaky sinusoidal endothelium of the liver versus the impermeable vessels of the brain. However, the transcriptional networks governing endothelial organ specialization remain unclear. Here we profile the accessible chromatin and transcriptional landscapes of the endothelium from the mouse liver, lung, heart, kidney, brain and retina, across developmental time, to identify potential transcriptional regulators of endothelial heterogeneity. We then determine which of these putative regulators are conserved in human brain endothelial cells, and using single-cell transcriptomic profiling, we define which regulatory networks are active during brain maturation. Finally, we show that the putative transcriptional regulators identified by these three approaches molecularly and functionally reprogram naive endothelial cells. Thus, this resource can be used to identify potential transcriptional regulators controlling the establishment and maintenance of organ-specific endothelial specialization.","PeriodicalId":74245,"journal":{"name":"Nature cardiovascular research","volume":" ","pages":"473-495"},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12023908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Left ventricular entry during catheter ablation reduces risk of brain lesions. 导管消融时左心室进入可降低脑损伤风险。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00646-w

Xiulin Koehler

引用次数: 0

Defining the transcriptional basis of organ-specific endothelial cell specialization. 定义器官特异性内皮细胞特化的转录基础。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00620-6

引用次数: 0

CD248⁺ fibroblasts drive cardiac fibrosis by interacting with immune cells. CD248+成纤维细胞通过与免疫细胞相互作用驱动心脏纤维化。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00625-1

引用次数: 0

Single-nucleus multi-omics implicates androgen receptor signaling in cardiomyocytes and NR4A1 regulation in fibroblasts during atrial fibrillation. 单核多组学涉及心房颤动期间心肌细胞中的雄激素受体信号和成纤维细胞中的NR4A1调节。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 Epub Date: 2025-03-25 DOI: 10.1038/s44161-025-00626-0

Francis J A Leblanc, Chi Him Kendrick Yiu, Lucia M Moreira, Aaron M Johnston, Neelam Mehta, Antonios Kourliouros, Rana Sayeed, Stanley Nattel, Svetlana Reilly, Guillaume Lettre

{"title":"Single-nucleus multi-omics implicates androgen receptor signaling in cardiomyocytes and NR4A1 regulation in fibroblasts during atrial fibrillation.","authors":"Francis J A Leblanc, Chi Him Kendrick Yiu, Lucia M Moreira, Aaron M Johnston, Neelam Mehta, Antonios Kourliouros, Rana Sayeed, Stanley Nattel, Svetlana Reilly, Guillaume Lettre","doi":"10.1038/s44161-025-00626-0","DOIUrl":"10.1038/s44161-025-00626-0","url":null,"abstract":"The dysregulation of gene expression programs in the human atria during persistent atrial fibrillation (AF) is not completely understood. Here, we reanalyze bulk RNA-sequencing datasets from two studies (N = 242) and identified 755 differentially expressed genes in left atrial appendages of individuals with persistent AF and non-AF controls. We combined the bulk RNA-sequencing differentially expressed genes with a left atrial appendage single-nucleus multi-omics dataset to assign genes to specific atrial cell types. We found noncoding genes at the IFNG locus (LINC01479, IFNG-AS1) strongly dysregulated in cardiomyocytes. We defined a gene expression signature potentially driven by androgen receptor signaling in cardiomyocytes from individuals with AF. Cell-type-specific gene expression modules suggested an increase in T cell and a decrease in adipocyte and neuronal cell gene expression in AF. Lastly, we showed that reducing NR4A1 expression, a marker of a poorly characterized human atrial fibroblast subtype, fibroblast activation markers, extracellular matrix remodeling and cell proliferation decreased.","PeriodicalId":74245,"journal":{"name":"Nature cardiovascular research","volume":" ","pages":"433-444"},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic molecular atlas of cardiac fibrosis at single-cell resolution shows CD248 in cardiac fibroblasts orchestrates interactions with immune cells. 单细胞分辨率心脏纤维化动态分子图谱显示，心脏成纤维细胞中的CD248协调与免疫细胞的相互作用。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 Epub Date: 2025-03-27 DOI: 10.1038/s44161-025-00617-1

Guohua Li, Cheng Ni, Jiacheng Wang, Feimu Zhang, Zaiyang Fu, Lingjun Wang, Biqing Wang, Ye Liu, Jing Zhao, Mo Li, Hao Lin, Fei Liao, Shuchang Ye, Yu Zhang, Jiayue Cai, Shaohui Shi, Zhiwei Zhong, Yanna Shi, Junhua He, Xushen Xiong, Yang Xu, Jinghai Chen, Wei Zhu, Yibin Wang, Jian'an Wang, Xinyang Hu

{"title":"Dynamic molecular atlas of cardiac fibrosis at single-cell resolution shows CD248 in cardiac fibroblasts orchestrates interactions with immune cells.","authors":"Guohua Li, Cheng Ni, Jiacheng Wang, Feimu Zhang, Zaiyang Fu, Lingjun Wang, Biqing Wang, Ye Liu, Jing Zhao, Mo Li, Hao Lin, Fei Liao, Shuchang Ye, Yu Zhang, Jiayue Cai, Shaohui Shi, Zhiwei Zhong, Yanna Shi, Junhua He, Xushen Xiong, Yang Xu, Jinghai Chen, Wei Zhu, Yibin Wang, Jian'an Wang, Xinyang Hu","doi":"10.1038/s44161-025-00617-1","DOIUrl":"10.1038/s44161-025-00617-1","url":null,"abstract":"Post-injury remodeling is a complex process involving temporal specific cellular interactions in the injured tissue where the resident fibroblasts play multiple roles. Here, we performed single-cell and spatial transcriptome analysis in human and mouse infarcted hearts to dissect the molecular basis of these interactions. We identified a unique fibroblast subset with high CD248 expression, strongly associated with extracellular matrix remodeling. Genetic Cd248 deletion in fibroblasts mitigated cardiac fibrosis and dysfunction following ischemia/reperfusion. Mechanistically, CD248 stabilizes type I transforming growth factor beta receptor and thus upregulates fibroblast ACKR3 expression, leading to enhanced T cell retention. This CD248-mediated fibroblast-T cell interaction is required to sustain fibroblast activation and scar expansion. Disrupting this interaction using monoclonal antibody or chimeric antigen receptor T cell reduces T cell infiltration and consequently ameliorates cardiac fibrosis and dysfunction. Our findings reveal a CD248+ fibroblast subpopulation as a key regulator of immune-fibroblast cross-talk and a potential therapy to treat tissue fibrosis.","PeriodicalId":74245,"journal":{"name":"Nature cardiovascular research","volume":" ","pages":"380-396"},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammatory CD248⁺ activated fibroblasts are a potential therapeutic target in ischemic heart disease. 炎性CD248+激活的成纤维细胞是缺血性心脏病的潜在治疗靶点。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00632-2

Joel G Rurik

引用次数: 0

Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice. 小鼠自发和刺激心脏再生的基因追踪和地形。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 Epub Date: 2025-03-07 DOI: 10.1038/s44161-025-00623-3

Ilaria Secco, Ana Backovic, Mateusz Tomczyk, Antonio Mura, Gang Li, Francesca Bortolotti, Simone Vodret, Matteo Dal Ferro, Elena Chiavacci, Lorena Zentilin, Gianfranco Sinagra, Serena Zacchigna, Miguel Mano, Mauro Giacca

{"title":"Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice.","authors":"Ilaria Secco, Ana Backovic, Mateusz Tomczyk, Antonio Mura, Gang Li, Francesca Bortolotti, Simone Vodret, Matteo Dal Ferro, Elena Chiavacci, Lorena Zentilin, Gianfranco Sinagra, Serena Zacchigna, Miguel Mano, Mauro Giacca","doi":"10.1038/s44161-025-00623-3","DOIUrl":"10.1038/s44161-025-00623-3","url":null,"abstract":"Despite recent efforts to stimulate endogenous cardiomyocyte proliferation for cardiac regeneration, the lack of reliable in vivo methods for monitoring cardiomyocyte replication has hindered our understanding of its mechanisms. Thymidine analogs, used to label proliferating cells, are unsuitable for long-term cardiac regeneration studies as their DNA incorporation elicits a damage response, leading to their elimination. Here we present CycleTrack, a genetic strategy based on the transcriptional activation of Cre recombinase from a temporally regulated cyclin B2 promoter segment, for permanent labeling of cardiomyocytes passing through the G2/M phase. Using CycleTrack, we visualized cardiomyocyte turnover in neonatal and adult mice under various conditions, including pregnancy, increased ventricular afterload, and myocardial infarction. CycleTrack also provided visual and quantitative evidence of ventricular remuscularization following treatment with pro-regenerative microRNAs. We identify the subendocardium as a key site of mitotic activity and provide a mode of cardiomyocyte division along their short axis. CycleTrack is a powerful tool to monitor cardiomyocyte renewal during regenerative interventions.","PeriodicalId":74245,"journal":{"name":"Nature cardiovascular research","volume":" ","pages":"397-411"},"PeriodicalIF":9.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11994457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143588442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-assisted cell-free DNA screening predicts pre-eclampsia risk. 机器学习辅助无细胞DNA筛查预测先兆子痫风险。

IF 9.4

Nature cardiovascular research Pub Date : 2025-04-01 DOI: 10.1038/s44161-025-00647-9

Andrea Tavosanis

引用次数: 0