African Journal of Biotechnology最新文献

Establishment of an efficient regeneration protocol of six different varieties of wheat (Triticum aestivum L.) 建立六种不同小麦（Triticum aestivum L.）的高效再生方案

African Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.5897/ajb2023.17638

Chen Bo, Tahir Muhammad, Fatima, Zafar Sara, Li Hui, Li Jia, J. R. Arthur, Caiming Gou, M. A. Hayssam, Abbas Manzar

引用次数: 0

Distribution of cassava mosaic begomoviruses in the North-Western provinces of Democratic Republic of Congo 刚果民主共和国西北部省份木薯花叶病毒的分布情况

African Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.5897/ajb2023.17634

Likiti Kola Olivier, Kisuka Muvirirwa Rosine, Diko Eboma Guy-Roger, Bulonza Mulimbanya Jean-Claude, Bakelena Zeyimo Tony, Mubenga Kankonda Onésime, M. Godefroid

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The effect of clarification methods on quality attributes of sugarcane-watermelon wine fermented by palm wine yeast (Saccharomyces cerevisiae) 澄清方法对棕榈酒酵母（酿酒酵母）发酵的甘蔗西瓜酒质量属性的影响

African Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.5897/ajb2023.17632

Osebhajimhende Uguomore Edmond, Maduka Ndukwe, Ahaotu Ihuoma

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Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso 布基纳法索的革兰氏阴性杆菌通过产生亚胺培南酶（IMP）型碳青霉烯酶对碳青霉烯类产生多重耐药性

African Journal of Biotechnology Pub Date : 2024-02-29 DOI: 10.5897/ajb2023.17626

Yves Patrik Bouniounou Damis, Mètuor-Dabiré Amana, Yasmine Tiemtore Rahimatou, Sougue Serge, Rose Bonkoungou Pegdwende, S. Jacques

{"title":"Multi-resistance to carbapenems by the production of Imipenemase (IMP)-types carbapenemases in Gram-negative bacilli in Burkina Faso","authors":"Yves Patrik Bouniounou Damis, Mètuor-Dabiré Amana, Yasmine Tiemtore Rahimatou, Sougue Serge, Rose Bonkoungou Pegdwende, S. Jacques","doi":"10.5897/ajb2023.17626","DOIUrl":"https://doi.org/10.5897/ajb2023.17626","url":null,"abstract":"The growing prevalence of Imipenemase (IMP) metallo-beta-lactamases (MBL) producing strains of Gram-negative bacilli (GNB) pathogens is a real concern for clinicians in the light of therapeutic impasses driven. However, resistance genes encoding these enzymes are hardly documented in Burkina Faso. This study aims to show carbapenem-resistance mediated by the production of IMP-type carbapenemase in GNB clinical strains collected in Ouagadougou, Burkina Faso. Strains resistance profile to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined by the disk diffusion method. Classical polymerase chain reaction (PCR) was carried out to detect β-lactamase ( bla ) gene of IMP in resistant strains. Out of 158 GNB collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or to aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (45.1%; n=41) and Klebsiella pneumoniae (26.5% n=24). Among 32 resistant strains bla IMP gene positive (35.2%), Escherichia coli was the predominant species carrying resistance gene (18.7%, n=17/91). The findings strengthen the scarce existing scientific data on antimicrobial resistance mediated by metallo-beta-lactamases (MBL) in Burkina Faso.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"33 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140412315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Estimates of genetic variability in ultraviolet irradiated populations of summer squash 紫外线照射夏南瓜种群的遗传变异估计

African Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.5897/ajb2023.17624

I. K. Mervat

引用次数: 0

Typing of enterovirus identified from Moroccan mussels (Mytilus galloprovincialis Lamarck, 1819) by seroneutralization 通过血清中和对从摩洛哥贻贝（Mytilus galloprovincialis Lamarck, 1819）中鉴定出的肠道病毒进行分型

African Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.5897/ajb2023.17622

Meryem Idrissi Azzouzi Lalla, El Qazoui Maria, Oumzil Hicham, Naciri Mariam

引用次数: 0

Analytical performances description of the immunoturbidimetric method for the determination of HbA1c using Selectra Pro M automated system at the Institut National dHygine (INH) of Lom 洛姆国立血液研究所（INH）使用Selectra Pro M自动系统测定HbA1c的免疫比浊法的分析性能说明

African Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.5897/ajb2023.17604

Katawa Gnatoulma, Adjete Kossi, Marthe Amessoudji Oukoe, Halatoko Wemboo, Edlom Tchadie Pélagie, Bara Fagdeba, Dossou Irène, Nguepou Tchopba Christèle, Simplice Karou Damintoti, Ameyapoh Yovi

{"title":"Analytical performances description of the immunoturbidimetric method for the determination of HbA1c using Selectra Pro M automated system at the Institut National dHygine (INH) of Lom","authors":"Katawa Gnatoulma, Adjete Kossi, Marthe Amessoudji Oukoe, Halatoko Wemboo, Edlom Tchadie Pélagie, Bara Fagdeba, Dossou Irène, Nguepou Tchopba Christèle, Simplice Karou Damintoti, Ameyapoh Yovi","doi":"10.5897/ajb2023.17604","DOIUrl":"https://doi.org/10.5897/ajb2023.17604","url":null,"abstract":"HbA1c is the best indicator for monitoring glycemic control in both type 1 and type 2 diabetic patients. Its value allows us to predict the development of long-term degenerative complications of diabetes. Several techniques offered by different laboratories allow the determination of HbA1c based on different principles. The purpose of the present study was to perform the verification of the analytical performances of the Selectra Pro M automated system, using immunoturbidimetry as the HbA1c assay method. Risk assessment of the method was performed; the analytical performances of the assay process was evaluated and the immunoturbidimetric method on the Selectra Pro M was compared to the capillary method on the Capillarys 2 Flex Piercing. Reproducibility and intermediate precision were satisfactory with CV in the range of 1.78 to 1.96% for the low level of Internal Quality Control (IQC) (HbA1c = 5.4%) and 0.82 to 2.28% for the high level of IQC (HbA1c = 11.3%). The reagent was linear from 2.5 to 16% (4 to 151 mmol/mol). The accuracy was considered satisfactory. The linear regression calculation (least squares line) showed an excellent correlation (R 2 = 0.96) between the two techniques with an equation of the type [Capillarys] = 1.13 [Selectra Pro M] - 0.83. All documented and tested performances correspond to the performance required for HbA1c assay at INH of Lomé.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"480 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification of stilbene synthase from Phaseolus vulgaris and determination of its catalytic activity 从粗壮黄豆中纯化二苯乙烯合成酶并测定其催化活性

African Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.5897/ajb2023.17629

Yongkang Yan, Zhanyou Yan, Guofang Zhao

引用次数: 0

Morphological traits variation of cowpea (Vigna unguiculata L. Walp) grown in Cte dIvoire 科特迪瓦种植的豇豆（Vigna unguiculata L. Walp）的形态特征变异

African Journal of Biotechnology Pub Date : 2024-01-31 DOI: 10.5897/ajb2023.17623

Khady Doumbia, Yaya Nadiala Coulibaly, Bi Irié Roger Djè, Yao Raymond Koffi, Lassana Tioté, K. K. Koffi

{"title":"Morphological traits variation of cowpea (Vigna unguiculata L. Walp) grown in Cte dIvoire","authors":"Khady Doumbia, Yaya Nadiala Coulibaly, Bi Irié Roger Djè, Yao Raymond Koffi, Lassana Tioté, K. K. Koffi","doi":"10.5897/ajb2023.17623","DOIUrl":"https://doi.org/10.5897/ajb2023.17623","url":null,"abstract":"The genetic diversity of a genetic resource is an indicator of its richness and offers many possibilities for improvement. Cowpea is an important source of nutrients for humans and animals. This study aimed at assessing genetic diversity and relationships present among 135 accessions in the Centre National de Recherches Agronomiques (CNRA) collection, using qualitative traits. Traits analysis revealed significant genetic variation between the studied accessions through high Shannon diversity index. Estimated for 15 morphological characters with 0.80 mean value, H’ ranged from 0.55 to 1. Seed traits generally expressed mean high diversity index (0.70-0.89). Between 15 parameters assessed, 12 exhibited the most discriminating variability of collection. The three MCA factorial axes explained 19.085% of phenotypic variability. The trait seed colour being correlated to these axes could be used as a selection criterion for local cowpeas improvement. Based on MCA and HAC methods, these accessions were classified into three distinct groups. Containing most accessions, subgroup Ia includes all seeds, pod, and flower colours. Almost all accessions of subgroup Ib possessed cream-coloured, smooth and globose seeds. All cluster II accessions have a creeping habit. The CNRA collection being very diversified, it constitutes a useful resource for future cowpea improvement studies in Côte d'Ivoire.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"483 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140471698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used 可可豆（L.）五个精英基因型体细胞胚胎发生的优先表达与外植体类型和所用方案有关

African Journal of Biotechnology Pub Date : 2023-12-31 DOI: 10.5897/ajb2023.17568

Poh Konan Georges N’goran, E. Minyaka, K. M. Kouassi, Siaka Kone, N’dede Theodore Djeni, Christophe Kouamé, J. N’Zi

{"title":"Preferential expression of somatic embryogenesis in five elite genotypes of Theobroma cacao (L.) associated with explant type and protocols used","authors":"Poh Konan Georges N’goran, E. Minyaka, K. M. Kouassi, Siaka Kone, N’dede Theodore Djeni, Christophe Kouamé, J. N’Zi","doi":"10.5897/ajb2023.17568","DOIUrl":"https://doi.org/10.5897/ajb2023.17568","url":null,"abstract":"This study aims to identify the suitable subculturing time, culture media (protocols) and explants type suitable for somatic embryogenesis expression in five elite genotypes of Theobroma cacao L. Thus, petals and staminodes explants extracted from immature flower buds of genotypes (C1, C8, C9, C14, and C16) are first grown in different media (CIM and CDM) to assess the subculturing time. Then, successive transfers in EDM medium at every 4 weeks intervals until 24 weeks. To assess culture media preference, these genotypes were grown in four different protocols (I, II, III, and IV) for callus induction. They were then successively subcultured (four times at 4 weeks intervals) into EDM. About the subculture time, embryo expression is earlier in C1, C9, C14, and C16 genotypes (2nd and 4th weeks) than C8 genotype (12 and 16th weeks). However, C1, C14, and C16 (with staminodes explants) prefer protocol IV, while their petal explants prefer protocol II. Also, C9 staminodes explants prefer protocol III while the C9 and C8 petals explants prefer protocol I. This study has identified suitable explants and protocols for somatic embryo production in the tested genotypes. Moreover, this work has laid out a new strategy to overcome recalcitrance to the somatic embryogenesis of T. cacao genotypes.","PeriodicalId":7414,"journal":{"name":"African Journal of Biotechnology","volume":"74 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139130312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0