Journal of Experimental Pathology最新文献_第4页

Interactions between interferon and the human immunodeficiency virus. 干扰素与人类免疫缺陷病毒的相互作用。

Journal of Experimental Pathology Pub Date : 1990-01-01

H E Gendelman, L M Baca, J A Turpin, D C Kalter, C Dieffenbach, R M Friedman, M S Meltzer

引用次数: 0

The in vivo effect of cyclosporine A on macrophages. 环孢素A对巨噬细胞的体内作用。

Journal of Experimental Pathology Pub Date : 1990-01-01

Y Matsushima, T Baba

{"title":"The in vivo effect of cyclosporine A on macrophages.","authors":"Y Matsushima, T Baba","doi":"","DOIUrl":"","url":null,"abstract":"The macrophage disappearance reaction (MDR) was induced when muramyl dipeptide (MDP) was injected intraperitoneally into guinea pigs bearing macrophage-rich peritoneal exudate cells. Heparin could inhibit the MDR induced by MDP. In the present study, we tested the effect of the immunosuppressive agent cyclosporine A (CsA) on MDR. The MDR was significantly suppressed in guinea pigs given 20 or 100 mg/kg of CsA, although 5 mg/kg of CsA had no effect. The number of macrophages elicited by liquid paraffin was significantly reduced in guinea pigs given with 20 or 100 mg/kg of CsA, but not in those given 5 mg/kg of CsA. These results indicate that CsA could directly affect macrophages in vivo, through a relatively high dose was required. Cyclosporine A (CsA), a cyclic peptide of 11 amino acids, is a fungal metabolite with potent immunosuppressive properties. Numerous experimental and clinical trials have demonstrated its effectiveness in organ transplantation. It has been suggested that primary target cells of CsA were T-lymphocytes, and macrophages were not directly affected. However, recent studies in an in vitro system have shown that some functions of macrophage are affected by CsA. These include chemotaxis (Drath & Kahan, 1983), interleukin-1 generation (Bunjes et al., 1981), prostaglandin E production (Whisler et al., 1984) and procoagulant activity (Carlsen et al., 1985). However, the effect of CsA on macrophages has not been elucidated in vivo. The macrophage disappearance reaction (MDR) is an in vivo manifestation of cell-mediated immunity and/or delayed type hypersensitivity (Sonozaki et al., 1975). Furthermore, our previous study demonstrated a possibility that MDR was an in vivo manifestation of macrophage activation (Ochiya et al., 1982). Muramyl dipeptide (MDP; N-acetyl-muramyl-L-alanyl-D-isoglutamine), a synthetic analogue of water soluble components of bacterial cell wall peptidoglycans, is known to have the ability to activate macrophages (Nagao et al., 1979). In the present study, attempts were made to induce MDR by MDP and the effect of CsA on the MDR was studied.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"5 2","pages":"39-48"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial membrane fluidity changes in renal ischemia. 肾缺血时线粒体膜流动性的改变。

Journal of Experimental Pathology Pub Date : 1990-01-01

C E Irazu, P R Rajagopalan, J K Orak, C T Fitts, I Singh

引用次数: 0

Effects of proteolytic enzymes on mammary tumor growth: in vitro and in vivo suppression and cellular death of tumor cells. 蛋白水解酶对乳腺肿瘤生长的影响:体外和体内肿瘤细胞的抑制和细胞死亡。

Journal of Experimental Pathology Pub Date : 1990-01-01

C A Belzer, J F Cavon

{"title":"Effects of proteolytic enzymes on mammary tumor growth: in vitro and in vivo suppression and cellular death of tumor cells.","authors":"C A Belzer, J F Cavon","doi":"","DOIUrl":"","url":null,"abstract":"Proteolytic enzymes may have potential value in the prophylaxis of malignant tumor development. C3H/HEJ mice, used for their ability to produce spontaneous mammary tumors, were injected intraperitoneally (IP) with proteolytic enzyme hydrolysate at a dosage range of 0.038 to 0.462 mg/gm body weight. The injections were given every other day, once a day for six months. The pathology results showed suppression of growth, and necrosis (and in some cases encapsulation) of the mammary tumors in C3H/HEJ mice. Concurrently, SP 2/0-AG 14 cells grown in the presence of 0.25 mg enzyme/ml to 3.75 mg enzyme/ml of proteolytic enzymes, showed little cellular deterioration when the dosage range remained below 1 mg enzyme/ml. When dosage ranges were greater than 1 mg enzyme/ml, cellular necrosis occurred within three days of the addition of the proteolytic enzymes. These results demonstrate that the proteolytic enzymes used in these experiments were beneficial in preventing tumor development and prolonging survival of C3H/HEJ mice when used in the appropriate concentration range. A portion of these results were presented elsewhere (2nd Int. Biotechnol. Expo; Oct. 1989; San Francisco).","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"5 3","pages":"97-110"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13250546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical detection of c-myc oncoprotein in paraffin embedded tissues. 石蜡包埋组织c-myc癌蛋白的免疫组化检测。

Journal of Experimental Pathology Pub Date : 1990-01-01

K Pavelic, Z P Pavelic, D Denton, J Reising, M Khalily, H D Preisler

{"title":"Immunohistochemical detection of c-myc oncoprotein in paraffin embedded tissues.","authors":"K Pavelic, Z P Pavelic, D Denton, J Reising, M Khalily, H D Preisler","doi":"","DOIUrl":"","url":null,"abstract":"In almost all studies using paraffin embedded tissue, c-myc protein has been found in the cytoplasm of cells. Since the protein is normally localized in the nucleus it is difficult to determine which histochemical observations are real and which are artefactual. The study designed here evaluated several different methods of fixation prior to paraffin embedding in an attempt to identify which would prevent the diffuse of the protein out of the nucleus. Using various fixation procedures (formalin, paraformaldehyde, B-5, Zamboni and AMeX) we found that fixation in cold acetone (-20 degrees C) overnight followed by 2x15 min fixation in acetone at +4 degrees c and at room temperature, cleared in methyl benzoate and xylene (AMeX procedure) gives reproducible nuclear staining when a variety of normal and tumor tissues are treated with an anti c-myc protein antibody. This method was then compared to frozen sections. While there was no cytoplasmic staining in same tissue specimens in both AMeX processed and frozen sections, the tissue architecture was much better preserved in AMeX processed samples. Our data strongly suggest that AMeX fixation, originally developed for T and B lymphocyte antigens, should be used for immunolocalization of c-myc oncoprotein in paraffin embedded tissues.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"5 4","pages":"143-53"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13256714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue response to subtotal retinectomy in a rabbit model. 兔视网膜次全切除术后的组织反应。

Journal of Experimental Pathology Pub Date : 1990-01-01

R L Peiffer, I K Orgel, R M Lewen, D E Eifrig

引用次数: 0

Lipid peroxidation and oxidation of lignoceric acid in kidneys from thioridazine treated rats. 硫硝嗪处理大鼠肾脏脂质过氧化和木质素酸氧化。

Journal of Experimental Pathology Pub Date : 1990-01-01

G S Dhaunsi, A K Singh, J Orak, I Singh

引用次数: 0

Histological alterations in mice induced by Pseudomonas aeruginosa slime glycolipoprotein. 铜绿假单胞菌黏液糖脂蛋白诱导小鼠组织学改变。

Journal of Experimental Pathology Pub Date : 1990-01-01

H I Papadaki, G Dimitracopoulos, D Bonicos, G Petrou, E D Anastassiou

引用次数: 0

The role of interferon and tumor necrosis factor in the pathogenesis of AIDS. 干扰素和肿瘤坏死因子在艾滋病发病中的作用。

Journal of Experimental Pathology Pub Date : 1990-01-01

A S Lau, B R Williams

{"title":"The role of interferon and tumor necrosis factor in the pathogenesis of AIDS.","authors":"A S Lau, B R Williams","doi":"","DOIUrl":"","url":null,"abstract":"Cytokines including interferon (IFN) and tumor necrosis factor (TNF) are potent modulators of immune processes. They are synthesized in response to microbial infections and inflammation. TNF and IFN interact with other cytokines to elicit differentiation and cellular responses of specific target cells. In view of their multiple biological effects, we have postulated that dysregulation of IFN and TNF may contribute to the pathogenesis of HIV infection. Here, we review data showing that the expression of IFN-alpha receptors is down-regulated in patients with AIDS. As a consequence, HIV-infected cultured cells and cells from AIDS patients show hyporesponsiveness to IFN action. This could contribute to mechanisms by which HIV evades the antiviral activity of IFN-alpha in HIV-infected cells and raise the question of the usefulness of IFN-alpha in the treatment of end-stage AIDS. TNF is a major mediator of inflammation and sepsis and also is capable of inducing the replication of HIV. TNF synthesis and its receptor expression are upregulated by the acid-labile IFN-alpha subtype present in the sera of HIV-infected individuals. In addition, the acid-labile IFN present in AIDS sera may contribute to the pathophysiological changes in sepsis by rendering the cells from AIDS patients hypersensitive to endotoxin stimulation resulting in further synthesis of TNF. Thus aberrant regulation of these cytokines and their cognate receptors are likely contributing factors to the pathogenesis of AIDS.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"5 3","pages":"111-22"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12873661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cyclophosphamide on the Peyer's patches in MRL1/1 and NZB/WF1 mice. 环磷酰胺对MRL1/1和NZB/WF1小鼠Peyer’s斑块的影响。

Journal of Experimental Pathology Pub Date : 1990-01-01

H Yoshioka, T Sugiyama, T Kita

引用次数: 0