Journal of Experimental Pathology最新文献

Therapeutic Pathology- The Pathology of Tomorrow 治疗病理学--未来的病理学

Journal of Experimental Pathology Pub Date : 2024-03-01 DOI: 10.33696/pathology.5.045

Sushma P Kulkarni

引用次数: 0

Retesting of Neonatal Hearing Screening Before and During the COVID-19 Pandemic: A Longitudinal Study COVID-19 大流行之前和期间的新生儿听力筛查重测：纵向研究

Journal of Experimental Pathology Pub Date : 2024-03-01 DOI: 10.33696/pathology.5.046

Carolina Schmitz Tiezerin, Karina Mary de Paiva, Luciele Kauna Woide, L. Cigana, Marcos José Machado, Patrícia Haas

{"title":"Retesting of Neonatal Hearing Screening Before and During the COVID-19 Pandemic: A Longitudinal Study","authors":"Carolina Schmitz Tiezerin, Karina Mary de Paiva, Luciele Kauna Woide, L. Cigana, Marcos José Machado, Patrícia Haas","doi":"10.33696/pathology.5.046","DOIUrl":"https://doi.org/10.33696/pathology.5.046","url":null,"abstract":"Introduction: Universal Newborn Hearing Screening (UNHS) plays an essential role in the early identification of hearing loss in neonates. Risk factors for hearing impairment may include family history, prematurity, and exposure to ototoxic substances. Coronavirus Disease 2019 (COVID-19) might be a significant contributing factor affecting the structures of the inner ear. Objective: To assess the auditory follow-up process of retesting for UNHS before and during the COVID-19 pandemic among neonates from an Outpatient Auditory Health Service (SASA) in the state of Santa Catarina with SUS (Unified Health System) assistance. Methods: A retrospective longitudinal study analyzed data from neonates attended at a SUS Auditory Health Service (SASA) from January 2018 to December 2022. Information related to UNHS and retest outcomes was assessed. Data were analyzed using Microsoft Excel® and MedCalc® Statistical Software version 22.006, utilizing statistical measures and regression analyses to identify factors associated with UNHS failures and retesting. Results: A failure to retest rate of 2.6% in the right ear and 2.2% in the left ear was observed among evaluated neonates. The average age of mothers of neonates who did not pass the test was 33 years, while the overall average was 27 years. Failure to pass the retest and a longer interval between UNHS and retesting were associated with UNHS Initial Retest Default (IRD). There was an increase in dropout rates for UNHS retesting, and the time interval between UNHS and retesting was extended during the pandemic. Conclusion: Several factors, including the interval between tests, mothers' age, and medical conditions, influenced the retest outcomes. The pandemic led to a significant increase in dropout rates and extended time for retesting.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"27 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140280084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Antimicrobial Potential of Endophytic Fungi and GC-MS Metabolic Profiling of Cephalosporium sp., and Fusarium moniliforme 内生真菌的抗菌潜力评估及头孢菌素和镰刀菌的 GC-MS 代谢谱分析

Journal of Experimental Pathology Pub Date : 2023-11-21 DOI: 10.33696/pathology.4.043

Hafiza Farhat, Faizah Urooj, Nida Sohai̇l, Shahid Ullah, Muhammad Aamer

{"title":"Evaluation of Antimicrobial Potential of Endophytic Fungi and GC-MS Metabolic Profiling of Cephalosporium sp., and Fusarium moniliforme","authors":"Hafiza Farhat, Faizah Urooj, Nida Sohai̇l, Shahid Ullah, Muhammad Aamer","doi":"10.33696/pathology.4.043","DOIUrl":"https://doi.org/10.33696/pathology.4.043","url":null,"abstract":"From last few decades, microbes gained special attention residing inside plant tissues, now called endophyte. Studies proved that these microbes are able to produce biologically active metabolites and effective candidates against various pathogens. Endophytic fungi are a source of natural therapeutic products. Therefore, endophytic Fusarium species are isolated from different plants. These endophytic Fusarium species showed a potential against common laboratory bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhimurium and Bacillus subtilis). Fusarium moniliforme and Cephalosporium sp., were further selected to isolate compounds by GC-MS techniques. The mycelial extract of Cephalosporium sp., and oily fraction of filtrates of F. moniliforme revealed the presence of several therapeutic compounds based on the peak areas, molecular weights, Rt (retention times) and m/z (mass fragmentations). The major bioactive metabolites identified from these fungi are Cholest-22-ene-21-ol, 3,5-dehydro-6-methoxy-pivalate, Salicylamide, 1,2-Benzenedicarboxylic acid, Geranyl isovalerate, and diisooctyl ester, and reported to possess antibacterial, antioxidant, nematicidal, and antifungal potential. These results will lead to further in-depth research into the potential cause of plants endophytes interactions. The wide application of fungal origin bio products offers an effective prospect for discovering novel therapeutic agents in order to combat infectious agents as well as agricultural pests.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"34 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139253291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Understanding and Gaps in Knowledge of Chlamydia trachomatis Infection 目前对沙眼衣原体感染的理解和认识差距

Journal of Experimental Pathology Pub Date : 2023-11-21 DOI: 10.33696/pathology.4.042

G. Bastidas, D. Bastidas, G. Bastidas-Delgado

引用次数: 0

Concurrent PIK3CA and TERT Mutation Promote the Proliferation and Invasion of Thyroid Carcinoma Cells and may be Caused by Up-regulating the Expression of GABPA/GABPB1 PIK3CA和TERT同时突变促进甲状腺癌细胞的增殖和侵袭，可能是通过上调GABPA/GABPB1的表达造成的

Journal of Experimental Pathology Pub Date : 2023-08-22 DOI: 10.33696/pathology.4.041

Huanli Duan, Qiang Ma, Leiming Wang, Shengnan Wang, Yanlei Xiong, Lianghong Teng

{"title":"Concurrent PIK3CA and TERT Mutation Promote the Proliferation and Invasion of Thyroid Carcinoma Cells and may be Caused by Up-regulating the Expression of GABPA/GABPB1","authors":"Huanli Duan, Qiang Ma, Leiming Wang, Shengnan Wang, Yanlei Xiong, Lianghong Teng","doi":"10.33696/pathology.4.041","DOIUrl":"https://doi.org/10.33696/pathology.4.041","url":null,"abstract":"Our previous research demonstrated that TERT and concurrent PIK3CA mutations predict worse overall survival in patients with poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma. However, the molecular mechanism underlying the synergistic oncogenic operations of the two oncogenes is unclear. This study aimed to explore further the effect of TERT and PIK3CA co-mutation on the malignant biological phenotype of thyroid carcinoma and its possible mechanism. PIK3CA E545K mutation plasmid was transfected into thyroid anaplastic cancer cell line (C643) with TERT promoter mutation, then CCK-8 and transwell invasion assays were used to investigate the ability of cell proliferation and invasion, respectively. RT-qPCR and western blot were performed to detect the expression of PIK3CA, TERT, GABPA and GABPB1. GABPA/GABPB1 siRNA plasmid was transfected with C643 cells, then the ability of cell proliferation and invasion were identified. We also detected the expression of PIK3CA and TERT. C643 cells carry TERT promoter mutation C228T. Concurrent PIK3CA E545K and TERT mutation markedly enhanced the proliferation and invasion of C643 cell in vitro, with significantly increased mRNA/protein expression of PIK3CA, TERT, GABPA and GABPB1. Knocking down GABPA markedly inhibited cell proliferation. Knocking down of GABPB1 significantly decreased the proliferation and invasion of C643 cells, with much lower expression of PIK3CA and TERT. TERT and PIK3CA co-mutations promote the proliferation and invasion of thyroid anaplastic carcinoma cells and may be caused by up-regulating the expression of GABPA and GABPB1.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"134 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

News About the Extracellular Vesicles from Mesenchymal Stem Cells: Functions, Therapy and Protection from COVID-19. 来自间充质干细胞的细胞外囊泡:功能、治疗和对COVID-19的保护

Journal of Experimental Pathology Pub Date : 2021-01-01 DOI: 10.33696/pathology.2.015

Jacopo Meldolesi

{"title":"News About the Extracellular Vesicles from Mesenchymal Stem Cells: Functions, Therapy and Protection from COVID-19.","authors":"Jacopo Meldolesi","doi":"10.33696/pathology.2.015","DOIUrl":"https://doi.org/10.33696/pathology.2.015","url":null,"abstract":"This is a Commentary of a review about extracellular vesicles of immune cells published two years ago in Clinical and Experimental Immunology, a prestigious journal of the field. The aim is to establish whether, and to what extent, results in scientific area of the review have been extended and strengthened by innovative findings of considerable interest. The analysis of the recently published results has revealed that in various areas of the review developments have occurred. However, innovative findings have been only about the extracellular vesicles secreted by mesenchymal stem cells, usually indicated as MSC-EVs. Based on these findings, the Commentary has been focused on recent MSC-EVs findings presented in three Sections dealing with 1. recently appeared, relevant functions of the latter vesicles; 2. therapeutic processes developed according well known criteria, however innovative in many respects; and 3. protection of COVID-19 disease patients from organ lesions induced by the specific virus, SARS-CoV-2, during the disease. As everybody knows, the COVID-19 pandemic started at the end of 2019, thus after the publication of the aforementioned review. Data of Section 3 are therefore innovative, of great potential interest also at the clinical level, applied by translational medicine to various organs, from lung to brain, heart, kidney, immune and other cells. In view of its relevance, the author expects that research and medical use of MSC-EV, active at present, will be further developed, acquiring additional relevance in the near future.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"2 1","pages":"47-52"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10292786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mechanistic and Translational Advances Using iPSC-Derived Blood Cells. 利用 iPSC 衍生血细胞的机制和转化进展。

Journal of Experimental Pathology Pub Date : 2020-01-01 DOI: 10.33696/pathology.1.010

Christopher S Thom, Stella T Chou, Deborah L French

引用次数: 0

Succinate Accumulation Links Mitochondrial MnSOD Depletion to Aberrant Nuclear DNA Methylation and Altered Cell Fate. 琥珀酸积累将线粒体MnSOD消耗与异常核DNA甲基化和细胞命运改变联系起来。

Journal of Experimental Pathology Pub Date : 2020-01-01 Epub Date: 2020-12-09

Kimberly L Cramer-Morales, Collin D Heer, Kranti A Mapuskar, Frederick E Domann

{"title":"Succinate Accumulation Links Mitochondrial MnSOD Depletion to Aberrant Nuclear DNA Methylation and Altered Cell Fate.","authors":"Kimberly L Cramer-Morales, Collin D Heer, Kranti A Mapuskar, Frederick E Domann","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies showed that human cell line HEK293 lacking mitochondrial superoxide dismutase (MnSOD) exhibited decreased succinate dehydrogenase (SDH) activity, and mice lacking MnSOD displayed significant reductions in SDH and aconitase activities. Since MnSOD has significant effects on SDH activity, and succinate is a key regulator of TET enzymes needed for proper differentiation, we hypothesized that SOD2 loss would lead to succinate accumulation, inhibition of TET activity, and impaired erythroid precursor differentiation. To test this hypothesis, we genetically disrupted the SOD2 gene using the CRISPR/Cas9 genetic strategy in a human erythroleukemia cell line (HEL 92.1.7) capable of induced differentiation toward an erythroid phenotype. Cells obtained in this manner displayed significant inhibition of SDH activity and ~10-fold increases in cellular succinate levels compared to their parent cell controls. Furthermore, SOD2 -/- cells exhibited significantly reduced TET enzyme activity concomitant with decreases in genomic 5-hmC and corresponding increases in 5-mC. Finally, when stimulated with δ-aminolevulonic acid (δ-ALA), SOD2 -/- HEL cells failed to properly differentiate toward an erythroid phenotype, likely due to failure to complete the necessary global DNA demethylation program required for erythroid maturation. Together, our findings support the model of an SDH/succinate/TET axis and a role for succinate as a retrograde signaling molecule of mitochondrial origin that significantly perturbs nuclear epigenetic reprogramming and introduce MnSOD as a governor of the SDH/succinate/TET axis.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"1 2","pages":"60-70"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876477/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25372406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-HHV-6 antibodies in normal population and in cancer patients in India. 印度正常人群和癌症患者的抗hhv -6抗体

Journal of Experimental Pathology Pub Date : 1992-01-01

K R Shanavas, V Kala, D M Vasudevan, T Vijayakumar, M Yadav

引用次数: 0

Aortic lysosomal hydrolases and pathological alterations in hypertensive and/or atherogenic diet fed rhesus monkeys. 高血压和/或动脉粥样硬化性饮食喂养恒河猴的主动脉溶酶体水解酶和病理改变。

Journal of Experimental Pathology Pub Date : 1992-01-01

G S Dhaunsi, S Majumdar, R N Chakravarti

引用次数: 0