Journal of Experimental Pathology最新文献_第5页

Preclinical toxicity study of streptozocin infused into the internal carotid artery of dogs and baboons. 犬、狒狒颈动脉内注射链脲佐菌素的临床前毒性研究。

Journal of Experimental Pathology Pub Date : 1990-01-01

P M Kanter, G A Bullard, C R West, Z P Pavelic

引用次数: 0

Anatomical and functional effects of estrogen-induced prolactinomas on the rat hypothalamus. 雌激素诱导的大鼠下丘脑泌乳素瘤的解剖和功能影响。

Journal of Experimental Pathology Pub Date : 1989-01-01

N Azad, R Nayyar, J Tentler, M Cernius, N V Emanuele, L Kirsteins, A M Lawrence

{"title":"Anatomical and functional effects of estrogen-induced prolactinomas on the rat hypothalamus.","authors":"N Azad, R Nayyar, J Tentler, M Cernius, N V Emanuele, L Kirsteins, A M Lawrence","doi":"","DOIUrl":"","url":null,"abstract":"Although estrogen-induced prolactinomas have been widely studied, little attention has been accorded to local pressure effects of the tumor on the hypothalamus and portal vasculature. To portray the magnitude of this phenomenon, four groups of 12-13-week-old female Fisher 344 rats were studied. Group 1 was an intact control receiving a subcutaneously (SC) placed placebo pellet; group 2 was an ovariectomized control with a SC placed placebo pellet; group 3 was ovariectomized with a 10 mg SC placed diethylstilbestrol (DES) pellet; and group 4 was ovariectomized receiving both 10 mg DES and 10 mg SC placed bromocriptine pellets. Blood samples were obtained at 4 weeks, and the animals were sacrificed at 8 weeks after pellet implantation at which time blood, pituitary and hypothalami were obtained. At 4 weeks serum prolactin levels were similarly and significantly elevated above the control groups in both the DES and DES/bromocriptine groups. By 8 weeks, however, serum prolactin level(s) in the DES-treated animals had tripled from the 4-week value, while levels in the DES/bromocriptine-treated animals were unchanged from the 4-week values. This finding matched the observation that the DES-treated animals had pituitaries 2.5-fold heavier than the DES/bromocriptine animals. The gross and histologic structure of the hypothalami and portal vessels were markedly disrupted in DES-treated rats and much less so in the DES/bromocriptine-treated group. These findings lead us to speculate that the pathogenesis of DES-induced prolactinomas proceeds in two phases: First, there is an early chemical induction phase in which estrogen directly and indirectly stimulates lactotrope proliferation and, second, a mechanical disinhibition phase, where tumor-induced destruction of the hypothalamus and portal vessels unleashes the pituitary from the dopaminergic restraining effects of the hypothalamus.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 4","pages":"237-49"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13755462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Matrix vesicles and calcification of rachitic rat osteoid. 棘突大鼠类骨的基质囊泡与钙化。

Journal of Experimental Pathology Pub Date : 1989-01-01

T F Johnson, D C Morris, H C Anderson

{"title":"Matrix vesicles and calcification of rachitic rat osteoid.","authors":"T F Johnson, D C Morris, H C Anderson","doi":"","DOIUrl":"","url":null,"abstract":"Tibiae from rachitic weanling rats were studied during healing to examine the mechanism of bone mineralization. Rickets was induced by feeding the animals a low phosphate, low vitamin D diet for five weeks. Calcification was reinstituted in three ways; group I animals received 1.0 ml of NaH2PO4 i.p. and the rachitogenic diet in a darkened room; group II animals were placed in a lighted room and given standard laboratory chow; group III animals were placed in lighted room, given standard laboratory chow and received 1.0 ml of 0.1M NaH2PO4 i.p. Group I healed slowly while II and III were found to heal rapidly and at nearly identical rates. Groups II and III revealed a significantly elevated serum alkaline phosphatase activity and became hyperphosphatemic as the rickets healed suggesting a more vigorous recovery. Ultrastructurally, numerous matrix vesicles were noted in unhealed rachitic bone matrix and these structures acquired mineral upon initiation of healing. Vesicle-associated mineral aggregates increased in size penetrating through and beyond the vesicle membrane and were incorporated into the advancing mineralization front. By 48 hrs. post-healing initiation, the osteoid borders were almost completely mineralized in groups II and III. These observations suggest that in addition to pre-existing mineral, matrix vesicles can also serve as nucleating sites in the osteoid of post-fetal bone.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 3","pages":"123-32"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13909118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of fatty acids and their derivatives on mitochondrial structures. 脂肪酸及其衍生物对线粒体结构的影响。

Journal of Experimental Pathology Pub Date : 1989-01-01

A K Singh, Y Yoshida, A J Garvin, I Singh

{"title":"Effect of fatty acids and their derivatives on mitochondrial structures.","authors":"A K Singh, Y Yoshida, A J Garvin, I Singh","doi":"","DOIUrl":"","url":null,"abstract":"Reye's Syndrome (RS) is characterized by encephalopathy, fatty degeneration of viscera and high levels of free fatty acids which are implicated in cellular toxicity. We have examined the effects of octanoic acid (C8:0), palmitic acid (C16:0) and oleic acid (C18:1) on rat liver mitochondrial swelling by spectroscopic and microscopic techniques. Of the fatty acids tested oleic acid had a greater effect than palmitic acid while octanoic acid had no effect. Acyl-CoA derivatives produced greater mitochondrial swelling than either acyl-carnitine or free fatty acids. However, identical amounts of palmitoyl-CoA and oleoyl-CoA were required to produce the same degree of swelling. Addition of carnitine (2mM) to oleoyl-CoA reduced the mitochondrial swelling significantly thus suggesting that the toxicity of the fatty acids may be reduced by conversion to their carnitine derivatives. Twice the concentration of oleoyl-carnitine was required to produce half the maximum swelling as compared to oleoyl-CoA. Ultrastructural profiles of mitochondria treated with oleic acid, oleoyl-CoA and oleoyl-carnitine demonstrated greater swelling with oleoyl-CoA than with oleic acid or oleoyl-carnitine. These results suggest that carnitine may protect the mitochondria from damage by the fatty acids and their acyl-CoA derivatives in Reye's Syndrome.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 1","pages":"9-15"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13916659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staining of surface antigen on migrating lymphocytes in the chemotaxis membrane. I: Complement C5 attracts helper/inducer T cell much more than suppressor/cytotoxic T cell and NK cell. 趋化膜中移行淋巴细胞表面抗原染色。补体C5对辅助/诱导剂T细胞的吸引作用远大于抑制/细胞毒性T细胞和NK细胞。

Journal of Experimental Pathology Pub Date : 1989-01-01

Y Hayashi, A Imaizumi, T Yoshida, M Torisu

{"title":"Staining of surface antigen on migrating lymphocytes in the chemotaxis membrane. I: Complement C5 attracts helper/inducer T cell much more than suppressor/cytotoxic T cell and NK cell.","authors":"Y Hayashi, A Imaizumi, T Yoshida, M Torisu","doi":"","DOIUrl":"","url":null,"abstract":"We have measured various chemotactic activities for neutrophils (1-3), lymphocytes (4), macrophages (5) and eosinophils (6-11) by the modified Boyden chamber method. In the conventional method, it is necessary to purify the indicator cells in upper chamber because it is impossible to distinguish inflammatory cells on the chemotactic membrane. For example, when we estimate T lymphocyte chemotaxis, we collected nylon-wool nonadherent peripheral blood lymphocytes (PBL) and confirmed their purity by sheep erythrocytes rosette formation assay. Pohajdak et al (12) evaluated NK cell chemotaxis by using large granular lymphocytes (LGL) for indicator cells. LGL fractionation was confirmed by monoclonal antibodies, HNK-1, OKT11, OKT3, OKT4, OKT8, OKM1, and MO2. When we arranged the study to evaluate chemotaxis of lymphocyte subsets which were divided by surface antigens, the conventional method needed a large quantity of PBL for the indicator cells. Moreover, one or two lymphocyte subsets had to be obtained from an identical PBL donor. This was time consuming. So, we tried to identify migrating lymphocytes in the chemotaxis membrane by their surface antigens. In this communication, we report that the staining of surface antigens on migrating lymphocytes in the chemotaxis membrane is effective and useful for evaluation of lymphocyte subset chemotaxis. It prompted us to report the new findings about modification of lymphocyte subset chemotaxis by C5a.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 3","pages":"181-92"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13926687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of Kupffer cells in initiating liver injury during endotoxemia. 库普弗细胞在内毒素血症中引发肝损伤的潜在作用。

Journal of Experimental Pathology Pub Date : 1989-01-01

M Katoh, M Torisu

引用次数: 0

In vivo detection of retroviral particles in mice with hybridoma induced ascites. 杂交瘤诱导腹水小鼠体内逆转录病毒颗粒的检测。

Journal of Experimental Pathology Pub Date : 1989-01-01

A H Bartal, R M Ludatscher, C Lichtig

引用次数: 0

ras oncogene p21 expression in hepatocellular carcinoma. Ras癌基因p21在肝细胞癌中的表达。

Journal of Experimental Pathology Pub Date : 1989-01-01

J Jagirdar, A Nonomura, J Patil, A Thor, F Paronetto

引用次数: 0

Ethanol treatment inhibits the development of diethylnitrosamine-induced tumors in rats. 乙醇处理抑制二乙基亚硝胺诱导的大鼠肿瘤的发展。

Journal of Experimental Pathology Pub Date : 1989-01-01

J Mandl, G Bánhegyi, T Garzó, K Lapis, F Antoni, Z Schaff

引用次数: 0

Characterization of the antigen-reactive T cell line mediating in vivo delayed type hypersensitivity established by antigen-induced interleukin 2. 抗原反应性T细胞系介导由抗原诱导的白细胞介素2建立的体内延迟型超敏反应的特性。

Journal of Experimental Pathology Pub Date : 1989-01-01

K Kobayashi, S Cohen, T Yoshida

{"title":"Characterization of the antigen-reactive T cell line mediating in vivo delayed type hypersensitivity established by antigen-induced interleukin 2.","authors":"K Kobayashi, S Cohen, T Yoshida","doi":"","DOIUrl":"","url":null,"abstract":"The murine antigen-reactive T cell line has been established by antigen-induced interleukin 2 (IL-2) derived from immunized syngeneic mice. The surface markers of the cells showed Thy 1+, and Lyt 1+2-. The antigen-reactive cells require the presence of IL-2 for continuous proliferation. IL-2 from mouse, rat, or human sources all stimulate the T cell line growth. The T cell mitogen such as concanavalin A and antigen alone do not stimulate cell proliferation of the cells. The cells proliferate antigen-specifically in the presence of both IL-2 and antigen-presenting cells from histocompatible mice. Antigen-specific in vivo delayed type hypersensitivity response is inducible by injection of the T cells into naive syngeneic mice along with IL-2 and antigen. The reactivity of the T cell line found in vitro (proliferation) and in vivo (delayed type hypersensitivity) is highly antigen-specific, because the cells do not respond another antigen used for immunizing mice. The antigen-reactive T cell line produces neither IL-2 nor inhibiting factors such as neutralizing factors against preformed IL-2 activity and IL-2 production inhibiting factors, thus the cells are exclusive IL-2 acceptor. These results suggest that the interaction of antigen-induced IL-2 and IL-2 dependent antigen-reactive T cells may play a significant role in the induction/expression of in vivo delayed type hypersensitivity to specific antigen, because antigen-induced IL-2 is probably only a relevant IL-2 in the immunized mice.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 3","pages":"163-79"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13926686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0