Journal of Experimental Pathology最新文献_第6页

Histopathological lesions produced by P. aeruginosa lipopolysaccharide in rats. 铜绿假单胞菌脂多糖对大鼠的组织病理损伤。

Journal of Experimental Pathology Pub Date : 1989-01-01

G N Tzanakakis, D K Veronikis, E D Anastasiou, K S McCully, G Dimitracopoulos

{"title":"Histopathological lesions produced by P. aeruginosa lipopolysaccharide in rats.","authors":"G N Tzanakakis, D K Veronikis, E D Anastasiou, K S McCully, G Dimitracopoulos","doi":"","DOIUrl":"","url":null,"abstract":"The lipopolysaccharide (LPS) of Pseudomonas aeruginosa is considered to be less toxic than the LPS from the Enterobacteriaceae family. The present study was undertaken to determine the time course of lesions produced in the lungs, spleen, liver, kidneys and bone marrow from 1 to 168 hours following a single (1.5mg) intravenous injection of P. aeruginosa LPS--1R (S-form), and its mutant strains, 557 (R-form) and 605 (more-R-form)--in rats. The lesions consisted of atelectasis, infiltration by polymorphonuclear leukocytes, intraalveolar hemorrhage and perivascular edema of the lung, hyperplasia of the white pulp of the spleen, necrosis of hepatic parenchyma, vacuolization of cells of the adrenal zone fasciculata, and hyperplasia of bone marrow. Immunoperoxidase stain for the R-form and the more-R forms of LPS of P. aeruginosa showed similar distribution kinetics within hepatocytes and Kupffer cells, while the S-form required 24 hours to become evident within hepatocytes. The data show that all 3 LPS preparations are toxic, distributed differently within hepatocytes, as demonstrated by immunoperoxidase stains, and exhibit different time courses and severity of lesions within the affected organs.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 4","pages":"199-211"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13661563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between c-myc gene protein, nucleic acids and hepatitis B virus expression in hepatoma cell lines and their corresponding tumors in nude mice. 裸鼠肝癌细胞系及相应肿瘤中c-myc基因蛋白及核酸与乙型肝炎病毒表达的关系

Journal of Experimental Pathology Pub Date : 1989-01-01

W R Zhai, F Paronetto

引用次数: 0

Sequence specific inhibition of in vitro translation of mutated or normal ras p21. 突变或正常ras p21体外翻译的序列特异性抑制。

Journal of Experimental Pathology Pub Date : 1989-01-01

Z P Yu, D F Chen, R J Black, K Blake, P O Ts'o, P Miller, E H Chang

{"title":"Sequence specific inhibition of in vitro translation of mutated or normal ras p21.","authors":"Z P Yu, D F Chen, R J Black, K Blake, P O Ts'o, P Miller, E H Chang","doi":"","DOIUrl":"","url":null,"abstract":"Antisense methylphosphonate-modified oligomers (ONMP) complementary to 8 nucleotides spanning the twelfth amino acid codon of human c-Ha-ras have been synthesized to explore their inhibitory effect on ras p21 translation. The ONMP Ras 0, perfectly complementary to the specific target region in normal c-Ha-ras, inhibits cell-free translation of p21 from a normal c-Ha-ras mRNA template in a dose-dependent manner. At 200uM Ras 0, p21 translation is inhibited by almost 90% in a rabbit reticulocyte lysate; at 100uM, p21 is reduced by over 60%. Ras I and Ras II contain, internally, a single and double base mismatch, respectively. The inhibitory activity of these ONMPs is reduced in proportion to the number of mismatches. When the target mRNA encodes the activated c-Ha-ras differing by a single nucleotide at the twelfth amino acid codon from normal c-Ha-ras, the magnitude of the inhibitory effect of Ras I increased significantly because Ras I is now perfectly complementary to its target mRNA. In turn, Ras 0, now only partially complementary, is considerably less effective at the same concentrations. Therefore, the inhibitory effect is exquisitely sensitive to the extent of the sequence complementarity between the antisense ONMP and the targeted mRNA.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 2","pages":"97-108"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13811688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of IL-1 and tumor necrosis factor by endothelial cells: role in stimulating fibroblast PGE2 synthesis. 内皮细胞表达IL-1和肿瘤坏死因子:在刺激成纤维细胞PGE2合成中的作用。

Journal of Experimental Pathology Pub Date : 1989-01-01

W R Shanahan, W W Hancock, J H Korn

引用次数: 0

Visualization of vascular platelet aggregation by plastic embedding and light microscopy. 用塑料包埋和光学显微镜观察血管血小板聚集。

Journal of Experimental Pathology Pub Date : 1989-01-01

S Berliner, Z Fishelson, M Ben-Bassat, B Frisch

{"title":"Visualization of vascular platelet aggregation by plastic embedding and light microscopy.","authors":"S Berliner, Z Fishelson, M Ben-Bassat, B Frisch","doi":"","DOIUrl":"","url":null,"abstract":"Previous studies have indicated that platelets play a role in inflammation and microvascular damage but routine histologic preparations do not permit clear visualization of the platelets in tissues. Plastic embedding was used in this study to demonstrate platelet aggregates in the pulmonary vasculature of mice exposed to complement activation. The degree of platelet aggregation in the excised lungs was graded semiquantitatively in a total of 75 mice. Control Balb/c mice had a mean aggregation score of 0.16 while mice which received 0.3 ml zymosan-activated plasma (ZAP) intravenously (iv) had a score of 2.2. Injection of 0.3 or 0.4 ml of ZAP to which epsilon-aminocaproic acid was added prior to incubation with zymosan resulted in a score of 2.6 or 4.7 respectively. Balb/c (C5 sufficient) and AKR/J (C5 deficient) mice injected iv with 1 mg zymosan had scores of 2.9 and 3.2, respectively. Cobra venom factor (CVF) injected iv to Balb/c mice induced a dose-dependent aggregation. Taken together, these results confirm that complement activation products can mediate intrapulmonary platelet aggregation and they also demonstrate the suitability of plastic embedding for visualization of intravascular platelet aggregates under light microscopy.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 2","pages":"79-85"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13909115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Journal of Experimental Pathology Pub Date : 1989-01-01

R P Nayyar, R Oslapas, E Paloyan

{"title":"Age related correlation between serum TSH and thyroid C cell hyperplasia in Long-Evans rats.","authors":"R P Nayyar, R Oslapas, E Paloyan","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of the present investigation was to determine whether there is a correlation between the serum levels of thyroid stimulating hormone (TSH), parafollicular cell (C) population in the thyroid and calcitonin (CT) secretion in aging Long-Evans (L-E) rats. Serum TSH and CT values were determined in 50 male rats at ages 2, 6, 12, 18 and 24 months and 50 female rats at 18, 21 and 24 months. The animals were sacrificed at the end of the experiment to evaluate thyroid C cell pathology. Male rats showed an increase in the serum TSH values at 6 and 12 months of age, a decline at 18 months and a significant increase from 18 to 24 months of age. In female rats, a significant increase in serum TSH concentration was also noted from 18 to 24 months of age. In both sexes, 24 month old animals with thyroid C cell hyperplasia (CH+ group) had a significantly higher level of serum TSH as compared to animals with normal distribution of C cells (CH- group). Concentration of serum CT showed a progressive increase with age in both sexes. Male rats with thyroid C cell pathology had significantly higher levels of serum CT at 24 months of age as compared to rats with normal C cell distribution. In female rats, however, serum CT concentrations in two groups were not statistically significant. We conclude from these studies that in aged L-E rats, serum TSH concentration has an influence on thyroid C cell population.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 2","pages":"87-95"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13909116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microvascular endothelium: a major target site of endotoxin induced injury in 10 day old rat. 微血管内皮:10日龄大鼠内毒素损伤的主要靶部位。

Journal of Experimental Pathology Pub Date : 1989-01-01

R P Nayyar, R M Hurley, M Goto, W P Zeller

{"title":"Microvascular endothelium: a major target site of endotoxin induced injury in 10 day old rat.","authors":"R P Nayyar, R M Hurley, M Goto, W P Zeller","doi":"","DOIUrl":"","url":null,"abstract":"We have studied the effects of two endotoxins, S enteritidis and E coli on liver, pancreas, intestine, lung and kidney of 10 day old Sprague Dawley rat at light and electron microscope levels. One group of experimental animals (N = 31) received a single intraperitoneal injection of 0.1 mg/kg of S enteritidis endotoxin. The second experimental group (N = 34) received multiple intraperitoneal injections of 5 to 10 mg/kg of E coli endotoxin at 15 minute intervals over a period of 75 minutes. Within four hours of injections, histopathologic evaluation of all the tissues revealed interstitial edema, widening of blood capillary lumina, accumulation of polymorphonuclear leukocytes and platelets in capillary lumina and marked swelling, vacuolization and focal desquamation of endothelial cells in microvasculature. Hepatocytes showed loss of glycogen, vacuolization and degeneration in the centro-portal region. Islet cells of pancreas also revealed swelling and vacuolization. In the small intestine, hemorrhagic pools of blood were frequently seen in lamina propria and the apical portion of villi showed degeneration and breakdown. Lungs showed focal hemorrhage, collapse of alveolar architecture and swelling of endothelium in larger artereoles. Based on these studies, we suggest that endothelial cells of microvasculature in various tissues of 10 day old rat are extremely sensitive to endotoxins, irrespective of source, and cells derived from reticuloendothelial system may play an important role in the endotoxicosis.","PeriodicalId":73745,"journal":{"name":"Journal of Experimental Pathology","volume":"4 2","pages":"57-67"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13811687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C-type virus particles in placentas of rhesus monkeys after maternal treatment with recombinant leukocyte A interferon. 重组白细胞A干扰素治疗后恒河猴胎盘中的c型病毒颗粒。

Journal of Experimental Pathology Pub Date : 1989-01-01

D Feldman, T Valentine, W H Niemann, R M Hoar, M Cukierski, A Hendrickx

引用次数: 0

Cytokines and oncogenes. 细胞因子和致癌基因。

Journal of Experimental Pathology Pub Date : 1989-01-01

R J Black

引用次数: 0

Electron microscopic characterisation of retrovirus and retrovirus-like particles induced by demethylating agents (5-azacytidine and 5-azadeoxycytidine) in Syrian hamster (Mesocricetus auratus) cells. 叙利亚仓鼠(Mesocricetus auratus)细胞中去甲基化剂(5-氮杂胞苷和5-氮杂脱氧胞苷)诱导的逆转录病毒和逆转录病毒样颗粒的电镜特征

Journal of Experimental Pathology Pub Date : 1989-01-01

J Lasneret, J Lesser, L Dianoux, M Canivet, P Bittoun, J Peries

引用次数: 0